| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,374,038 | 0 | T | C | 27.3% | 34.0 / 10.9 | 22 | F273L (TTC→CTC) ‡ | ycjO | putative ABC transporter membrane subunit YcjO |
| Reads supporting (aligned to +/- strand): ref base T (4/12); new base C (0/6); total (4/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.41e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TCTCTACAAAACCGCCTTTGCCTTTAATGATTTAGGAAAAGCGGCGGCGATCTCGGTGGTGCTCTTCATCATTATTTTCGCTGTCATTCTGCTGACCAGGAAACGGGTGAACCTCAATGGCAACAAA > NC_000913/1373974‑1374100 | tctcTACAAAACCGCCTTTGCCTTTAATGATTTAGGAAAAGCGGCGGCGGTCTCGGTGGGGgtct > 1:251688/1‑65 (MQ=255)tctcTACAAAACCGCCTTTGCCTTTAATGATTTAGGAAAAGCGGCGGCGGTCTCGGGGGGGctct > 1:1509983/1‑65 (MQ=255)tctcTACAAAACCGCCTTTGCCTTTAATGATTTAGGAAAAGCGGCGGCGGTCTCGGGGGGGctct > 1:2540643/1‑65 (MQ=255)tctcTACAAAACCGCCTTTGCCTTTAATGATTTAGGAAAAGCGGCGGCGCTCTCGGTGGTGctct > 1:449037/1‑65 (MQ=255)tctcTACAAAACCGCCTTTGCCTTTAATGATTTAGGAAAAGCGGCGGCGATCTCGGTGGTGctct > 1:2547946/1‑65 (MQ=255)tctcTACAAAACCGCCTTTGCCTTTAATGATTTAGGAAAAGCGGCGGCGATCTCGGTGGTGctct > 1:2560289/1‑65 (MQ=255)tctcTACAAAACCGCCTTTGCCTTTAATGATTTAGGAAAAGCGGCGGCGATCTCGGTGGGGgtct > 1:1288710/1‑65 (MQ=255)tctcTACAAAACCGCCTTTGCCTTTAATGATTTAGGAAAAGCGGCGGCGATCTCGGTGGGGgtct > 1:2872590/1‑65 (MQ=255)tctcTACAAAACCGCCTTTGCCTTTAATGATTTAGGAAAAGCGGCGGCGATCTCGGGGGGGgtct > 1:3468581/1‑65 (MQ=255)tctcTACAAAACCGCCTTTGCCTTTAATGATTTAGGAAAAGCGGCGGCGATCTCGGGGGGGctct > 1:2172818/1‑65 (MQ=255)tctcTACAAAACCGCCTTTGCCTTTAATGATTTAGGAAAAGCGGCGGCGATCTCGGGGGGGctct > 1:609204/1‑65 (MQ=255) tctACAAAACCGCCTTTGCCTTTAATGATTTAGGAAAAGCGGCGGCGATCTCGGTGGTGCTCTtc > 1:1783070/1‑65 (MQ=255) tACAAAACCGCCTTTGCCTTTAATGATTTAGGAAAAGCGGCGGCGATCTCGGTGGTGCTCTtcat > 1:517852/1‑65 (MQ=255) aaaaCCGCCTTTGCCTTTAATGATTTAGGAAAAGCGGCGGCGATCTCGGTGGGGCTCTTcatcat > 1:257622/1‑65 (MQ=255) aCCGCCTTTGCCTTTAATGATTTAGGAAAAGCGGCGGCGATCTCGGTGGGGCTCTTCATCattat > 1:3078270/1‑65 (MQ=255) cctttgcctttAATGATTTAGGAAAAGCGGCGGCGATCTCGGTGGTGCTCTTCATCATTATTTTc < 1:2885741/65‑1 (MQ=255) tccctttAATGATGTAGAGGAAGGCGTGTAGATCTCGGTGGT‑CGCCGTATCATTATTTTCGCTGt < 1:775754/63‑1 (MQ=255) cctttAATGATTTAGGAAAAGCGGCGGCGATCTCGGTGGTGCTCTTCATCATTATTTTCGCTGTc < 1:3000231/65‑1 (MQ=255) cctttAATGATTTAGGAAAAGCGGCGGCGATCTCGGTGGTGCTCTTCATCATTATTTTCGCTGTc < 1:913171/65‑1 (MQ=255) cctttAATGATTTAGGAAAAGCGGCGGCGATCTCGGTGGTGCTCTTCATCATTATTTTCGCTGTc < 1:1647721/65‑1 (MQ=255) aTGGTTTGGGAACAGCGGGGTCTATCTCGGTGGT‑CGCCGTATCATTATTTTCGCTGTCATTctgc < 1:3636034/65‑1 (MQ=255) aTGATTTAGGAAAAGCGGCGGCGGTCTCGGTGGGGCTCTTCATCATTATTTTCGCTGTCATTctg > 1:3390791/1‑65 (MQ=255) aTGATTTAGGAAAAGCGGCGGCGATCTCGGGGGTGCTCTTTATCATTATTTTTTCTGTCCTTctg > 1:1372727/1‑65 (MQ=255) tAGGAAAAGCGGCGGCGATCTCGGTGGTGCTCTTCATCATTATTTTCGCTGTCATTCTGCTGAcc < 1:2110860/65‑1 (MQ=255) gggaaaGCCGTGTCGATCTCGGTGGT‑CGCCGTATCATTATTTTCGCTGTCATTCTGCTGACCAgg < 1:3305366/62‑1 (MQ=255) gAAAAGCGGCGGCGATCTCGGTGGTGCTCTTCATCATTATTTTCGCTGTCATTCTGCTGACCAgg < 1:2546199/65‑1 (MQ=255) gAAAAGCGGCGGCGATCTCGGTGGTGCTCTTCATCATTATTTTCGCTGTCATTCTGCTGACCAgg < 1:2724001/65‑1 (MQ=255) gAAAAGCGGCGGCGATCTCGGTGGTGCTCTTCATCATTATTTTCGCTGTCATTCTGCTGACCAgg < 1:2032715/65‑1 (MQ=255) ggggcggcgATCTCGGTGGT‑CGCCGTATCATTATTTTCGCTGTCATTCTGCTGACCAGGAAACgg < 1:1897720/63‑1 (MQ=255) gtagATCTCGGTGGT‑CGCCGTATCATTATTTTCGCTGTCATTCTGCTGACCAGGAAACGGGTGaa < 1:2964375/62‑1 (MQ=255) cgATCTCGGTGGTGCTCTTCATCATTATTTTCGCTGTCATTCTGCTGACCAGGAAACGGGTGaaa > 1:3567092/1‑64 (MQ=255) cgATCTCGGTGGT‑CGCCGTATCATTATTTTCGCTGTGATTCTGCTGACCAGGAAACGGGTGAAcc < 1:2695256/65‑1 (MQ=255) agATCTCGGTGGT‑CGCCGTATCATTATTTTCGCTGTCATTCTGCTGACCAGGAAACGGGTGAAcc < 1:3381143/64‑1 (MQ=255) agATCTCGGTGGT‑CGCCGTATCATTATTTTCGCTGTCATTCTGCTGACCAGGAAACGGGTGAAcc < 1:1968438/64‑1 (MQ=255) gATCTCGGTGGTGCTCTTCATCATTATTTTCGCTGTCATTCTGCTGACCAGGAAACGGGTGAAcc < 1:534383/65‑1 (MQ=255) gATCTCGGTGGTGCTCTTCATCATTATTTTCGCTGTCATTCTGCTGACCAGGAAACGGGTGAAcc < 1:3129776/65‑1 (MQ=255) gATCTCGGTGGTGCTCTTCATCATTATTTTCGCTGTCATTCTGCTGACCAGGAAACGGGTGAAcc < 1:52208/65‑1 (MQ=255) aTCTCGGTGGTGCTCTTCATCATTATTTTCGCTGTCATTCTGCTGACCAGGAAACGGGTGAACCt < 1:1232952/65‑1 (MQ=255) gtggtgCTCTTCATCATTATTTTCGCTGTCATTCTGCTGACCAGGAAACGGGTGAACCTCAATgg > 1:1245228/1‑65 (MQ=255) gtggtgCTCTTCATCATTATTTTCGCTGTCATTCTGCTGACCAGGAAACGGGTGAACCTCAACgg > 1:1256593/1‑65 (MQ=255) ggtgCTCTTCATCATTATTTTCGCTGTCATTCTGCTGACCAGGAAACGGGTGAACCTCAATGGca > 1:11931/1‑65 (MQ=255) gctgCTCTTCATCATTATTTTCGCTGTCATTCTGCTGACCAGGAAACGGGTGAACCTCAATGGca < 1:2352283/63‑1 (MQ=255) ctctTCATCATTATTTTCGCTGTCATTCTGCTGACCAGGAAACGGGTGAACCTCAATGGcaacaa < 1:1489137/65‑1 (MQ=255) tctTCATCATTATTTTCGCTGTCATTCTGCTGACCAGGAAACGGGTGAACCTCAATGGCAACaaa > 1:1585231/1‑65 (MQ=255) tctTCATCATTATTTTCGCTGTCATTCTGCTGACCAGGAAACGGGTGAACCTCAATGGCAACaaa < 1:1821020/65‑1 (MQ=255) | TCTCTACAAAACCGCCTTTGCCTTTAATGATTTAGGAAAAGCGGCGGCGATCTCGGTGGTGCTCTTCATCATTATTTTCGCTGTCATTCTGCTGACCAGGAAACGGGTGAACCTCAATGGCAACAAA > NC_000913/1373974‑1374100 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |