| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,875,838 | 0 | A | C | 53.8% | ‑2.5 / 16.3 | 13 | T56P (ACC→CCC) | nimT | 2‑nitroimidazole exporter |
| Reads supporting (aligned to +/- strand): ref base A (5/1); new base C (0/7); total (5/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.66e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.59e-02 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
ACCGTTACTGGATACGATTCGTTCCGCTTACTCGCTGACGACAGCGCAAACCGGCTTATTGACCACCCTGCCATTATTGGCCTTTGCGCTAATCTCACCTTTGGCTGCCCCGGTAGCGCGACG > NC_000913/1875774‑1875896 | aCCGTTACTGGATACGATTCGTTCCGCTTACTCGCTGACGACAGCGCAAACCGGCTTATTGacca > 1:242388/1‑65 (MQ=255)aCCGTTACTGGATACGATTCGTTCCGCTTAATCGCTGACGAAAGCGCCAAACGGCTTATTGagca > 1:1110916/1‑65 (MQ=255) cGTTACTGGATACGATTCGTTCCGCTTACTCGCTGACGACAGCGCAAACCGGCTTATTGaccacc < 1:1838792/65‑1 (MQ=255) tGGATACGATTCGTTCCGCTTACTCGCTGACGACAGCGCAAACCGGCTTATTGACCACCCTGGCa > 1:2249158/1‑65 (MQ=255) gATGCGTTTCGTCCCGCTTCCTCGCTGCCGACAGCGCAACCCGGCTTATTGACCCCCCTGCCatt < 1:1476177/65‑1 (MQ=255) tcggATTCGTTCCGCTTACTCGCTGACGACAGCGCAAACCGGCTTATTGCCCCCCCTGCCattat < 1:46503/62‑1 (MQ=255) tACGATTCGTTCCGCTTCCTCGCTGACGACAGCGCAACCCGGCTTATTGACCCCCCTGCCattat < 1:950322/65‑1 (MQ=255) aCGATTCGTTCCGCTTACTCGCTGACGACAGGGCAAACCGGCTTATTGACCACCCTGCCattatt > 1:3421531/1‑65 (MQ=255) ttCCGCTTACTCGCTGACGACAGCGCAAACCGGCTTATTGACCACCCTGCCATTATTGGCCTTTg > 1:3289626/1‑65 (MQ=255) tCCACTTCCTCCCTGACAACGGCGCAATCGGGCTTATTGCCCCCCCTGCCATTATTGGCCTTTgc < 1:1677705/65‑1 (MQ=255) ttccTCGCTGACGACAGCGCAAACCGGTTTATTGACCCCCCTGCCATTATTGGCCTTTGCGCTaa < 1:3338815/62‑1 (MQ=255) ttACGCGCTGACGACAGCGCAAACCGGCTTATTAACCACCCTGCCATTATTGGCCTTTGCGCTaa < 1:3423993/65‑1 (MQ=255) tACCCGCTGACGACAGCGCAAACCGGCTTTTTGCCCCCCCTGCCATTATTGGCCTTTGCGCTAAt < 1:851042/65‑1 (MQ=255) tCGCTGACGACAGCGCAAACCGGCTTATTGACCACCCTGCCATTATTGGCCTTTGCGCTAAtctc > 1:2049540/1‑65 (MQ=255) gCTGACGACAGCGCAACCCGGCTTATTGACCCCCCTGCCATTATTGGCCTTTGCGCTAATCTCAc < 1:1151886/65‑1 (MQ=255) gCTGACGACAGCGCAAACCGGCTTATTGCCCACCCTGCCATTATTGGCCTTTGCGCTAATCTCAc < 1:2792210/65‑1 (MQ=255) cTGACGACAGCGCAACCCGGCTTATTGCCCCCCCTGCCATTATTGGCCTTTGCGCTAATCTCAcc < 1:3007554/65‑1 (MQ=255) acAGCGCAAACCGGCTTATTGACCACCCTGCCATTATTGGCCTTTGCGCTAATCTCACCTTTGGc > 1:2204336/1‑65 (MQ=255) acAGCGCAAACCGGCTTATTGACCACCCTGCCATTATTGGCCTTTGCGCTAATCTCACCTTTGGc > 1:1315701/1‑65 (MQ=255) aGCGCAAACCGGCTTATTGCCCCCCCTGCCATTATTGGCCTTTGCGCTAATCTCACCTTTGGCTg < 1:973497/65‑1 (MQ=255) aGCGCAAACCGGCTTATTGCCCCCCCTGCCATTATTGGCCTTTGCGCTAATCTCACCTTTGGCTg < 1:984439/65‑1 (MQ=255) aGCGAAAACCGGCTTTTTGCCCCCCCTGCCATTATTGGCCTTTGCGCTAATCTCACCTTTGGCTg < 1:2931193/65‑1 (MQ=255) aGCCCAACCCGCCTTATTGCCCCCCCTGCCATTATTGGCCTTTGCGCTAATCTCACCTTTGGCTg < 1:967287/65‑1 (MQ=255) gcAAACCGGCTTATTGACCACCCTGCCATTATTGGCCTTTGCGCTAATCTCACCTTTGGCTGccc > 1:2168901/1‑65 (MQ=255) acAAACCGGCTTATTGACCACCCTGCCATTATTGGCCTTTGCGCTAATCTCACCTTTGGCTGcca > 1:2229149/2‑64 (MQ=255) ttGCCCCCCCTGCCATTATTGGCCTTTGCGCTAATCTCACCTTTGGCTGCCCCGGTAGCGCGACg < 1:1006366/65‑1 (MQ=255) | ACCGTTACTGGATACGATTCGTTCCGCTTACTCGCTGACGACAGCGCAAACCGGCTTATTGACCACCCTGCCATTATTGGCCTTTGCGCTAATCTCACCTTTGGCTGCCCCGGTAGCGCGACG > NC_000913/1875774‑1875896 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |