| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,302,928 | 0 | T | G | 58.8% | ‑6.0 / 19.0 | 17 | V147G (GTG→GGG) | yhbV | putative peptidase YhbV |
| Reads supporting (aligned to +/- strand): ref base T (1/6); new base G (10/0); total (11/6) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.66e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.50e-02 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TTGCTCAAACAGGGCATGATGCGCTGGTGTATGCCAGTGGAGCTTTCCCGCGACTGGCTGGTGAATCTGCTTAATCAGTGCGATGAGCTGGGCATTCGTAACCAGTTTGAAGTGGAAGTCCTGAG > NC_000913/3302867‑3302991 | ttGCTCAAACAGGGCATGATGCGCTGGTGTATGCCAGTGGAGCTTTCCCGCGACTGGCTGGGGaa > 1:1298085/1‑65 (MQ=255) cATGATGCGCTGGTGTATGCCAGTGGAGCTTTCCCGCGACTGGCTGGGGGAACTGCTTAATCAgg > 1:322582/1‑64 (MQ=255) atgCGCTGGTGTATGCCAGTGGAGCTTTCCCGCGACTGGCTGGGGGATCTGGTTAAACCATGCGa > 1:2989137/1‑65 (MQ=255) gcgcTGGTGTATGCCAGTGGAGCTTTCCCGCGACTGGCTGGGGAATCTGCTTAATCAGTGCGATg > 1:473574/1‑65 (MQ=255) gcTGGTGTATGCCAGTGGAGCTTTCCCGCGACACGATGGAGAGGGTGGGTCATCAGTGCGATggg > 1:3485258/1‑63 (MQ=255) gcTGGGGTATGGCAGTGGAGCTTTCCCGCGACTGGCTGGTGAATCTGCTTAATCAGTGCGATGAg < 1:2147230/65‑1 (MQ=255) cTGGTGTATGCCAGTGGAGCTTTCCCGCGACTGGCTGGGGAATCTGCTTAATCAGTGCGATGAGc > 1:175388/1‑65 (MQ=255) ggTGTATGCCAGTGGAGCTTTCCCGCGACTGGCTGGGGAAACTGCTTAATCAGTGCGATGAggcg > 1:1686948/1‑62 (MQ=255) gtgtATGCCAGTGGAGCTTTCCCGCGACTGGCTGGTGAATCTGCTTAATCAGTGCGATGAGCTgg < 1:2961142/65‑1 (MQ=255) tGCCAGTGGAGCTTTCCCGCGACTGGCTGGTGAATCTGCTTAATCAGTGCGATGAGCTGGGCAtt < 1:3253156/65‑1 (MQ=255) gCCAGTGGAGCTTTCCCGCGACTGGCTGGTGGATTTGCTTTATCAGTGCGATGAGCTGGGCATTc > 1:909243/1‑65 (MQ=255) gCCAGTGGAGCTTTCCCGCGACTGGCTGGTGAATCTGCTTTATCAGTGCGATGGGCTGGGCATTc > 1:881681/1‑65 (MQ=255) gCCAGTGGAGCTTTCCCGCGACTGGCTGGTGAATCTGCTTAATTAATGCGATGAGCTGGGCATTc > 1:818477/1‑65 (MQ=255) gCCAGTGGAGCTTTCCCGCGACTGGCTGGTGAATCTGCTTAATCAGTGCGATGAGCCGGGCATTc > 1:2797272/1‑65 (MQ=255) gCCAGTGGAGCTTTCCCGCGACTGGCTGGTGAATCTGCTTAATCAGAGCGATGAGCCGGGCATTc > 1:2067751/1‑65 (MQ=255) gCCAGTGGAGCTTTCCCGCGACTGGCTGGGGAATTTGTTTAATCAGGGCGATGAGCTGGGGATTc > 1:3173066/1‑65 (MQ=255) gCCAGTGGAGCTTTCCCGCGACTGGCTGGGGAATCTGCTTAATCAGTGCGATGAGCTGGGCATTc > 1:647492/1‑65 (MQ=255) gCCAGTGGAGCTTTCCCGCGACTGGCTGGGGAATATTCTTGATCATGGCGAAGTGACGGGCAttt > 1:1624541/1‑64 (MQ=255) ccAGTGGAGCTTTCCCGCGACTGGCTGGGGGATCTGCTTAATCAGTGCGATGAGGTGGGCGTTCg > 1:527953/1‑65 (MQ=255) ccAGTGGAGCTTTCCCGCGACTGGCTGGGGGAATTGCTTTATTAGTGCGCTGAGCTGGGCAttgg > 1:1745636/1‑63 (MQ=255) ccAGTGGAGCTTTCCCGCGACTGGCTGGGGAATCTGCTTAATCAGTGCGATGAGCTGGGCATTCg > 1:3129083/1‑65 (MQ=255) ggAGCTTTCCCGCGACTGGCTGGGGAAGCCGCTTAATCAGTGCGATGAGCTGGGCATTCGTAAcc > 1:426362/1‑65 (MQ=255) ggAGCTTTCCCGCGACTGGCTGGCGAATCTGCTTAATCAGTGCGATGAGCTGGGCATTCGTAAcc > 1:3137805/1‑65 (MQ=255) aGCTTTCCCGCGACTGGCTGGGGAATCTGCTTAATCAGTGCGATGAGCTGGGCATTCGTAACCAg > 1:568041/1‑65 (MQ=255) aGCTTTCCCGCGACTGGCTGGGGAATCTGCTTAATCAGGGCGATGAGCTGGGGATTCGTAACCAg > 1:2835708/1‑65 (MQ=255) tttCCCGCGACTGGCTGGCGGATCTGCCTAATCAGTGCGATGAGCTGGGGATTCGTAACCAGttt > 1:2889228/1‑65 (MQ=255) gcgACTGGCTGGTGAATCTGCTTAATCAGTGCGATGAGCTGGGCATTCGTAACCAGTTTGAAGTg < 1:698348/65‑1 (MQ=255) ctggctggGGAATCTGCTTAATCAGTGCGATGAGCTGGGCATTCGTAACCAGTTTGAAGTGGAAg > 1:1164002/1‑65 (MQ=255) ggTGAATCTGCTTAGTCAGTGCGATGAGCTGGGCATTCGTAACCAGTTTGAAGTGGAAGTCCTGa < 1:2410536/65‑1 (MQ=255) gTGAATCTGCTTAATCAGTGCGATGAGCTGGGCATTCGTAACCAGTTTGAAGTGGAAGTCCTGAg < 1:2813033/65‑1 (MQ=255) gTGAATCTGCTTAATCAGTGCGATGAGCTGGGCATTCGTAACCAGTTTGAAGTGGAAGTCCTGAg < 1:2118175/65‑1 (MQ=255) | TTGCTCAAACAGGGCATGATGCGCTGGTGTATGCCAGTGGAGCTTTCCCGCGACTGGCTGGTGAATCTGCTTAATCAGTGCGATGAGCTGGGCATTCGTAACCAGTTTGAAGTGGAAGTCCTGAG > NC_000913/3302867‑3302991 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |