Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	49,765	C→T	intergenic (+134/‑58)	kefC → / → folA	K(+) : H(+) antiporter KefC/dihydrofolate reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	49,765	0	C	T	100.0%	167.2 / NA	45	intergenic (+134/‑58)	kefC/folA	K(+) : H(+) antiporter KefC/dihydrofolate reductase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (21/24);  total (21/24)

GGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGA  >  NC_000913/49703‑49826                                                               |                                                              ggAGAGAGCGTGGACTCGCCAGCCGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTtga                                                             >  1:1071800/1‑65 (MQ=255) ggAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCTGTctc                                                             >  1:972790/1‑63 (MQ=255) ggAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTtga                                                             >  1:541761/1‑65 (MQ=255) ggAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTtga                                                             >  1:1575082/1‑65 (MQ=255) ggAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTtga                                                             >  1:1658183/1‑65 (MQ=255) ggAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTtga                                                             >  1:1656146/1‑65 (MQ=255) ggAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTtga                                                             >  1:370292/1‑65 (MQ=255)     agagCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCAGCAGTTgacgac                                                         >  1:2611624/1‑65 (MQ=255)       agCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGACGACgg                                                       <  1:1633679/65‑1 (MQ=255)       agCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGACGACgg                                                       <  1:1952107/65‑1 (MQ=255)       agCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGACGACgg                                                       <  1:85085/65‑1 (MQ=255)       agCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGACGACgg                                                       <  1:1315892/65‑1 (MQ=255)       agCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGACGACgg                                                       <  1:2740627/65‑1 (MQ=255)        gCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGACGACGGt                                                      <  1:2750657/65‑1 (MQ=255)        gCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGACGACGGt                                                      <  1:2315939/65‑1 (MQ=255)        gCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGACGACGGt                                                      >  1:519278/1‑65 (MQ=255)        gCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGACGACGGt                                                      <  1:524339/65‑1 (MQ=255)        gCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGACGACGGt                                                      <  1:1724934/65‑1 (MQ=255)        gCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGACGACGGt                                                      <  1:83973/65‑1 (MQ=255)         cGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGACGACGGtt                                                     >  1:3050846/1‑65 (MQ=255)              aCTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGACGACGGTTTACgt                                                >  1:2004577/1‑64 (MQ=255)              aCTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGACGACGGTTTACGc                                                >  1:1790845/1‑65 (MQ=255)              aCTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGACGACGGTTTACGc                                                >  1:2000158/1‑65 (MQ=255)              aCTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGACGACGGTTTACGc                                                >  1:521686/1‑65 (MQ=255)              aCTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGACGACGGTTTACGc                                                <  1:2020059/65‑1 (MQ=255)              aCTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGACGACGGTTTACGc                                                >  1:2216809/1‑65 (MQ=255)              aCTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGACGACGGTTTACGc                                                <  1:3640750/65‑1 (MQ=255)              aCTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGACGACGGTTTACGc                                                >  1:3628480/1‑65 (MQ=255)              aCTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGACGACGGTTTACGc                                                >  1:2572078/1‑65 (MQ=255)               cTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGACGACGGTTTACGCt                                               >  1:1638927/1‑65 (MQ=255)                  gCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGACGACGGTTTACGCTTTa                                            <  1:109332/65‑1 (MQ=255)                  gCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGACAACGGTTTACGCTTTa                                            <  1:37439/65‑1 (MQ=255)                   ccAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGACGACGGTTTACGCTTTAc                                           <  1:1777509/65‑1 (MQ=255)                   ccAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGACGACGGTTTACGCTTTAc                                           <  1:1328646/65‑1 (MQ=255)                   ccAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGACGACGGTTTACGCTTTAc                                           >  1:2327506/1‑65 (MQ=255)                   ccAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGACGACGGTTTACGCTTTAc                                           >  1:2229531/1‑65 (MQ=255)                    cagcagAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGACGACGGTTTACGCTTTACg                                          >  1:985689/1‑65 (MQ=255)                     agcagAATATAAAATTTTCCTCAACGTCATCCTCGCACCAGTTGACGACGGTTTACGCTTTACGt                                         <  1:1798510/65‑1 (MQ=255)                     agcagAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGACGACGGTTTACGCTTTACGt                                         <  1:49837/65‑1 (MQ=255)                     agcagAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGACGACGGTTTACGCTTTACGt                                         <  1:3310192/65‑1 (MQ=255)                     agcagAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGACGACGGTTTACGCTTTACGt                                         <  1:2707645/65‑1 (MQ=255)                        agAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGACGACGGTTTACGCTTTACGtata                                      >  1:2456714/1‑65 (MQ=255)                        agAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGACGACGGCTTACGCTTTACGtata                                      >  1:2008092/1‑65 (MQ=255)                             ataAAATTTTCCTCAACATCATCCTCGCACCAGTTGACGACGGTTTACGCTTTACGTATAGTGGc                                 >  1:2981297/1‑65 (MQ=255)                             ataAAATTTTCCTCAACATCATCCTCGCACCAGTTGACGACGGTTTACGCTTTACGTATAGTGGc                                 >  1:1792073/1‑65 (MQ=255)                             ataAAATTTTCCTCAACATCATCCTCGCACCAGTTGACGACGGTTTACGCTTTACGTATAGTGGc                                 >  1:1325245/1‑65 (MQ=255)                                  aTTTTCCTCAACATCATCCTCGCACCAGTTGACGACGGTTTACGCTTTACGTATAGTGGCGACaa                            >  1:303756/1‑65 (MQ=255)                                  aTTTTCCTCAACATCATCCTCGCACCAGTTGACGACGGTTTACGCTTTACGTATAGTGGCGACaa                            >  1:2570901/1‑65 (MQ=255)                                  aTTTTCCTCAACATCATCCTCGCACCAGTTGACGACGGTTTACGCTTTACGTATAGTGGCGACaa                            >  1:2337846/1‑65 (MQ=255)                                  aTTTTCCTCAACATCATCCTCGCACCAGTTGACGACGGTTTACGCTTTACGTATAGTGGCGACaa                            >  1:2828421/1‑65 (MQ=255)                                    tttCCTCAACATCATCCTCGCACCAGTTGACGACGGTTTACGCTTTACGTATAGTGGCGACAAtt                          >  1:2259955/1‑65 (MQ=255)                                                catcCTCGCACCAGTTGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCggg              <  1:3213944/65‑1 (MQ=255)                                                   ccTCGCACCAGTTGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGaaa           >  1:2356903/1‑65 (MQ=255)                                                   ccTCGCACCAGTTGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGaaa           >  1:375864/1‑65 (MQ=255)                                                       gCACCAGTTGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAAtctc       <  1:365628/65‑1 (MQ=255)                                                       gCACCAGTTGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAAtctc       <  1:2784463/65‑1 (MQ=255)                                                           cAGTTGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATg   <  1:2796022/65‑1 (MQ=255)                                                           cAGTTGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATg   <  1:1590477/65‑1 (MQ=255)                                                           cAGTTGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATg   <  1:1687274/65‑1 (MQ=255)                                                            aGTTGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGa  <  1:1188161/65‑1 (MQ=255)                                                               |                                                              GGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGA  >  NC_000913/49703‑49826

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑