Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,390,754	G→T	A252A (GCC→GCA)	rsgA ←	ribosome small subunit‑dependent GTPase A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,390,754	0	G	T	100.0%	71.7 / NA	20	A252A (GCC→GCA)	rsgA	ribosome small subunit‑dependent GTPase A
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (13/7);  total (13/7)

ACTCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGTGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGATCTC  >  NC_000913/4390701‑4390813                                                      |                                                            aCTCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGTCgtggt                                                  >  1:1738482/1‑65 (MQ=255)  cTCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGTCgtggtg                                                 <  1:3573923/65‑1 (MQ=255)   tCCTGGGGAGTCAATCACATCCCCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGgtgt                                                <  1:3608911/65‑1 (MQ=255)   tCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGgtgt                                                <  1:2049296/65‑1 (MQ=255)   tCCGGGGGAGTCAATCACATCGCCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGgtgt                                                <  1:1420803/65‑1 (MQ=255)    ccTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGGtgtg                                               <  1:1868396/65‑1 (MQ=255)           aGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGGTGTGCTGACCg                                        >  1:745613/1‑65 (MQ=255)           aGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGGTGTGCTGACCg                                        >  1:2229631/1‑65 (MQ=255)           aGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGGTGTGCTGACCg                                        >  1:2071679/1‑65 (MQ=255)           aGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGGTGTGCTGACCg                                        >  1:568385/1‑65 (MQ=255)           aGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGGTGTGCTGACCg                                        >  1:1779715/1‑65 (MQ=255)           aGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGGTGTGCTGACCg                                        >  1:646576/1‑65 (MQ=255)           aGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGGTGGGCTGACCg                                        >  1:2821796/1‑65 (MQ=255)           aGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGTCGGGGAGTGCTGACCg                                        >  1:3213919/1‑65 (MQ=255)           aGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGTGTGCTGACCg                                        >  1:1497186/1‑65 (MQ=255)             tCAATCCCACCACGGCCGTGGGGGAAGTGATACAGCCGAGCTGGGGTCGTGGTGTGCTGACCGAg                                      <  1:1348122/65‑1 (MQ=255)              cAATCACATCCCCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGGTGTGCTGACCGAGc                                     <  1:2420198/65‑1 (MQ=255)                 tcacatcacCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGGTGTGCTGACCGAGcccc                                  >  1:273472/1‑65 (MQ=255)                      tcacCGCCGTGCGGGAAGCGATACAGCCGAGCTGCGGTCGTGGTGTGCTGACCGAGCCCCGAGtt                             >  1:3344682/1‑65 (MQ=255)                           gccgTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGGTGTGCTGACCGAGCCCCGAGTTGTCag                        >  1:2444372/1‑65 (MQ=255)                           gccgTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGGTGTGCTGACCGAGCCCCGAGTTGTCag                        >  1:486732/1‑65 (MQ=255)                           gccgTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGGTGTGCTGACCGAGCCCCGAGTTGTCag                        >  1:701356/1‑65 (MQ=255)                           gccgTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGGCGTGCTGACCGAGCCCCGAGTTGCCag                        >  1:167421/1‑65 (MQ=255)                              gTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGGTGTGCTGACCGAGCCCCGAGTTGTCAgaga                     <  1:1991623/65‑1 (MQ=255)                                  cagAAGTGATACAGCCGAGCTGCGGTCGTGGTGTGCTGACCGAGCCCCGAGTTGTCAGAGATATc                 <  1:2877074/63‑1 (MQ=255)                                           tACAGCCGCGCTGCGGGCGTGCTGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAg        <  1:35656/65‑1 (MQ=255)                                            aCAGCCGAGCTGCGGTCGTGGTGTGCTGGCCGAGCTCCGAGTTGTCAGAGATATCGTTGGTCAgg       <  1:3179273/65‑1 (MQ=255)                                            aCAGCCGAGCTGCGGTCGTGGTGTGCTGCCCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAgg       <  1:1369785/65‑1 (MQ=255)                                              aGCCGAGCTGCGGTCGTGGTGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGAt     >  1:1943175/1‑65 (MQ=255)                                                 cGAGCTGCGGTCGTGGTGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGAtctc  >  1:3628259/1‑65 (MQ=255)                                                      |                                                            ACTCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGTGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGATCTC  >  NC_000913/4390701‑4390813

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑