Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	49,884	C→T	P21L (CCG→CTG)	folA →	dihydrofolate reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	49,884	0	C	T	100.0%	30.6 / NA	10	P21L (CCG→CTG)	folA	dihydrofolate reductase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (6/4);  total (6/4)

AATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTG  >  NC_000913/49822‑49942                                                               |                                                           aaTGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCTGt                                                          <  1:1343194/65‑1 (MQ=255)    gATCAGTCTGTTTGTGTCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCTGTGGa                                                       <  1:864286/65‑1 (MQ=255)       cAGTCTGATTGCGGCGTTAGCGGTAGTTCGCGTTATCGGCATGGAAAACGCCATGCTGTGGAAcc                                                    <  1:2424220/65‑1 (MQ=255)                tGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCTGTGGAACCTGCCTGCCg                                           >  1:2682818/1‑65 (MQ=255)                      tttAGCGTTAGACCGCTTTATCGGCTTGAAAAACGCCATGCTGGGGACCCTGCCTGCCGATcgcg                                     <  1:860477/64‑1 (MQ=255)                             gTAGATCGCGTTATCGGCATGGAAAACGCCATGCTGTGGAACCTGCCTGCCGATCGCGCCTGGtt                              >  1:2614479/1‑65 (MQ=255)                             gTAGATCGCGTTATCGGCATGGAAAACGCCATGCTGTGGAACCTGCCTGCCGATCGCGCCTGGtt                              >  1:923060/1‑65 (MQ=255)                                 aTCGCGTTATCGGCATGGAAAACGCCATGCTGTGGAACCTGCCTGCCGATCGCGCCTGGTTTaaa                          >  1:3466163/1‑65 (MQ=255)                                   ctcgTTTTCCGCATGAAAACCGCCATCCTGTGGACCCTGCCTGACGATCGCGCCTGGTTTAAACg                        <  1:973081/63‑1 (MQ=255)                                    gcgTTATCGGCATGGAAAACGCCATGCTGTGGAACCTGCCTGCCGATCGCGCCTGGGTTAAAcct                       >  1:2901870/1‑63 (MQ=255)                                    gcgTTATCGGCATGGAAAACGCCATGCTGTGGAACCTGCCTGCCGATCGCGCCCGGTTTAAACGc                       >  1:2979699/1‑65 (MQ=255)                                        tATCGGCATGGAAAACGCCATGCTGTGGAACCTGCCTGCCGATCGCGCCTGGTTTAAACGCAaca                   >  1:3140217/1‑65 (MQ=255)                                         aTCGGCATGGAAAACGCCATGCTGTGGAACCTGCCTGCCGATCGCGCCTGGTTTAAACGCAacac                  <  1:1173521/65‑1 (MQ=255)                                                         ccATGCTGTGGACCCTGCCTGCCGATCGCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTg  <  1:3261204/65‑1 (MQ=255)                                                               |                                                           AATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTG  >  NC_000913/49822‑49942

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑