Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	49,905	T→G	L28R (CTC→CGC)	folA →	dihydrofolate reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	49,905	0	T	G	100.0%	73.7 / NA	22	L28R (CTC→CGC)	folA	dihydrofolate reductase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (19/3);  total (19/3)

GTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACC  >  NC_000913/49843‑49960                                                               |                                                        tttAGCGTTAGACCGCTTTATCGGCTTGAAAAACGCCATGCTGGGGACCCTGCCTGCCGATcgcg                                                       <  1:860477/64‑1 (MQ=255)        gTAGATCGCGTTATCGGCATGGAAAACGCCATGCTGTGGAACCTGCCTGCCGATCGCGCCTGGtt                                                >  1:923060/1‑65 (MQ=255)        gTAGATCGCGTTATCGGCATGGAAAACGCCATGCTGTGGAACCTGCCTGCCGATCGCGCCTGGtt                                                >  1:2614479/1‑65 (MQ=255)            aTCGCGTTATCGGCATGGAAAACGCCATGCTGTGGAACCTGCCTGCCGATCGCGCCTGGTTTaaa                                            >  1:3466163/1‑65 (MQ=255)              ctcgTTTTCCGCATGAAAACCGCCATCCTGTGGACCCTGCCTGACGATCGCGCCTGGTTTAAACg                                          <  1:973081/63‑1 (MQ=255)               gcgTTATCGGCATGGAAAACGCCATGCTGTGGAACCTGCCTGCCGATCGCGCCTGGGTTAAAcct                                         >  1:2901870/1‑63 (MQ=255)               gcgTTATCGGCATGGAAAACGCCATGCTGTGGAACCTGCCTGCCGATCGCGCCCGGTTTAAACGc                                         >  1:2979699/1‑65 (MQ=255)                   tATCGGCATGGAAAACGCCATGCTGTGGAACCTGCCTGCCGATCGCGCCTGGTTTAAACGCAaca                                     >  1:3140217/1‑65 (MQ=255)                    aTCGGCATGGAAAACGCCATGCTGTGGAACCTGCCTGCCGATCGCGCCTGGTTTAAACGCAacac                                    <  1:1173521/65‑1 (MQ=255)                                    ccATGCTGTGGACCCTGCCTGCCGATCGCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTg                    <  1:3261204/65‑1 (MQ=255)                                        gctgTGGAACCTGCCTGCCGATCGCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTa                >  1:2783518/4‑65 (MQ=255)                                        gctgTGGAACCTGCCTGCCGATCGCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTa                >  1:436146/4‑65 (MQ=255)                                         ctgTGGAACCTGCCTGCCGATCGCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTAt               >  1:2454342/3‑65 (MQ=255)                                             ggAACCTGCCTGCCGATCGCGCCTGGTTTAACCGCAACACCTTAAATAAACCCGTGATTATGGgc           >  1:1315331/1‑65 (MQ=255)                                             ggAACCTGCCTGCCGATCGCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGgc           >  1:1081467/1‑65 (MQ=255)                                               aaCCTGCCTGCCGATCGCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGgccg         >  1:934544/1‑65 (MQ=255)                                               aaCCTGCCTGCCGATCGCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGgccg         >  1:1052839/1‑65 (MQ=255)                                               aaCCTGCCTGCCGATCGCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGgccg         >  1:1427975/1‑65 (MQ=255)                                               aaCCTGCCTGCCGATCGCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGgccg         >  1:3642011/1‑65 (MQ=255)                                                aCCTGCCTGCCGATCGCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGgccgc        >  1:3337117/1‑65 (MQ=255)                                                aCCTGCCTGCCGATCGCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGgccgc        >  1:212355/1‑65 (MQ=255)                                                aCCTGCCTGCCGATCGCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGgccgc        >  1:2198266/1‑65 (MQ=255)                                                 cctgcctgCCGATCGCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGgccgcc       >  1:3557072/1‑65 (MQ=255)                                                  ctgcctgcCGATCGCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCa      >  1:3649486/1‑65 (MQ=255)                                                  ctgcctgcCGATCGCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCa      >  1:2910395/1‑65 (MQ=255)                                                  ctgcctgcCGATCGCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCa      >  1:2580735/1‑65 (MQ=255)                                                    gcctgccGATCGCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATa    >  1:2709678/1‑65 (MQ=255)                                                      ctgccGATCCCGCCTGTTTTAACCGCAACACCTAAAAAAACCCCGTGATTATGGGCCGCCATAcc  <  1:490975/65‑1 (MQ=255)                                                               |                                                        GTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACC  >  NC_000913/49843‑49960

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑