Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	481,400	C→T	A1002T (GCA→ACA)	acrB ←	multidrug efflux pump RND permease AcrB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	481,400	0	C	T	100.0%	49.3 / NA	15	A1002T (GCA→ACA)	acrB	multidrug efflux pump RND permease AcrB
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (8/7);  total (8/7)

GGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAACCAGCGGCATAACGCCGAGGATA  >  NC_000913/481336‑481458                                                                 |                                                           ggAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGc                                                            >  1:2281236/1‑65 (MQ=255) ggAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGCACCTACTGc                                                            >  1:296788/1‑65 (MQ=255)   aaCGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTgtgt                                                          >  1:1768812/1‑65 (MQ=255)                  cAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGTGTTCTGCGCGCCGGAAc                                           <  1:2848584/65‑1 (MQ=255)                  cAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGTGTTCTGCGCGCCGGAAc                                           <  1:615620/65‑1 (MQ=255)                    gTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGTGTTCTGAGCGCCGGAACCa                                         >  1:2296008/1‑65 (MQ=255)                      aCCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGTGTTCTGCGCGCCGGCACCAGc                                       >  1:1972763/1‑65 (MQ=255)                      aCCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGTGTTCTGCGCGCCGGAACCAGc                                       >  1:105933/1‑65 (MQ=255)                      aCCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGTGTTCTGCGCGCCGGAACCAGc                                       >  1:1545888/1‑65 (MQ=255)                      aCCGTTGCGGTCACCATCCCGCCCATTACACCGGGACCTACTGTGTTCTGCGCGCCGGAACCAGc                                       >  1:2221789/1‑65 (MQ=255)                         gTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGTGTTCTGCGCGCCGGAACCAGCAcc                                    <  1:1522074/65‑1 (MQ=255)                           tGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGTGTTCTGCGCGCCGGAACCAGCACCAg                                  >  1:2086793/1‑65 (MQ=255)                                 cACCATCCCGCCCATTACACCGGTACCTACTGTGTTCTGCGCGCCGGAACCAGCACCAGTACTGa                            <  1:2721853/65‑1 (MQ=255)                                      tCCCGCCCATTACACCGGTACCTACTGTGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAAcc                       >  1:2987008/1‑65 (MQ=255)                                      tCCCGCCCATTACACCGGTACCTACTGTGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAAcc                       >  1:2632883/1‑65 (MQ=255)                                            ccATTACACCGGTACCTACTGTGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAACCAGCGGc                 <  1:3604252/65‑1 (MQ=255)                                            ccATTACACCGGTACCTACTGTGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAACCAGCGGc                 >  1:984307/1‑65 (MQ=255)                                                  cacCGGTACCTCCTGTGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAACCAGCGGCATAACg           <  1:253187/65‑1 (MQ=255)                                                   acCGGTACCTACTGTGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAACCAGCGGCATAACGc          <  1:1455620/65‑1 (MQ=255)                                                   acCGGTACCTACTGTGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAACCAGCGGAATAACGc          >  1:1841776/1‑65 (MQ=255)                                                        tACCTACTGTGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAACCAGCGGCATAACGCCGAgg     <  1:2142246/65‑1 (MQ=255)                                                        tACCTACTGTGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAACCAGCGGCATAACGCCGAgg     <  1:5942/65‑1 (MQ=255)                                                           cTACTGTGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAACCAGCGGCATAACGCCGAGGATa  >  1:492185/1‑65 (MQ=255)                                                                 |                                                           GGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAACCAGCGGCATAACGCCGAGGATA  >  NC_000913/481336‑481458

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑