Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	485,614	G→T	N2K (AAC→AAA)	acrA ←	multidrug efflux pump membrane fusion lipoprotein AcrA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	485,614	0	G	T	100.0%	189.0 / NA	53	N2K (AAC→AAA)	acrA	multidrug efflux pump membrane fusion lipoprotein AcrA
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (38/15);  total (38/15)

TTAGGGCTAAGCTGCCTGAGAGCATCAGAACGACCGCCAGAGGCGTAAACCCTCTGTTTTTGTTCATATGTAAACCTCGAGTGTCCGATTTCAAATTGGTCAATGGTCAAAAGTTAATAAAC  >  NC_000913/485553‑485674                                                              |                                                             ttAGGGCTAAGCTGCCTGAGAGCATCAGAACGACCGCCAGAGGCGTAAACCCTCTGTTTTTTTTc                                                           >  1:2915794/1‑65 (MQ=255) ttAGGGCTAAGCTGCCTGAGAGCATCAGAACGACCGCCAGAGGCGTAAACCCTCTGTTTTTTTTc                                                           >  1:1534872/1‑65 (MQ=255)    gggCTAAGCTGCCTGAGAGCATCAGAACGACCGCCAGAGGCGTAAACCCTCTGTTTTTTTTCata                                                        <  1:2401778/65‑1 (MQ=255)     ggCTAAGCTGCCTGAGAGCATCAGAACGACCGCCAGAGGCGTAAACCCTCTGTTTTTTTTCatat                                                       >  1:2551905/1‑65 (MQ=255)      gCTAAGCTGCCTGAGAGCATCAGAACGACCGCCAGAGGCGTAAACCCTCTGTTTTTTTTCATATg                                                      >  1:2302802/1‑65 (MQ=255)      gCTAAGCTGCCTGAGAGCATCAGAACGACCGCCAGAGGCGTAAACCCTCTGTTTTTTTTCATATg                                                      >  1:1387745/1‑65 (MQ=255)      gCTAAGCTGCCTGAGAGCATCAGAACGACCGCCAGAGGCGTAAACCCTCTGTTTTTTTTCATATg                                                      <  1:2001471/65‑1 (MQ=255)      gCTAAGCTGCCTGAGAGCATCAGAACGACCGCCAGAGGCGTAAACCCTCTGTTTTTTTCCATATg                                                      >  1:576051/1‑65 (MQ=255)       cTAAGCTGCCTGAGAGCATCAGAACGACCGCCAGAGGCGTAAACCCTCTGTTTTTTTTCATATGt                                                     >  1:1217192/1‑65 (MQ=255)       cTAAGCTGCCTGAGAGCATCAGAACGACCGCCAGAGGCGTAAACCCTCTGTTTTTTTTCATATGt                                                     >  1:654015/1‑65 (MQ=255)       cTAAGCTGCCTGAGAGCATCAGAACGACCGCCAGAGGCGTAAACCCTCTGTTTTTTTTCATATGt                                                     >  1:178227/1‑65 (MQ=255)          aGCTGCCTGAGAGCATCAGAACGACCGCCAGAGGCGTAAACCCTCTGTTTTTTTTCATATGTaaa                                                  <  1:2863813/65‑1 (MQ=255)          aGCTGCCTGAGAGCATCAGAACGACCGCCAGAGGCGTAAACCCTCTGTTTTTTTTCATATGTaaa                                                  <  1:360866/65‑1 (MQ=255)          aGCTGCCTGAGAGCATCAGAACGACCGCCAGAGGCGTAAACCCTCTGTTTTTTTTCATATGTaaa                                                  <  1:1423477/65‑1 (MQ=255)          aGCTGCCTGAGAGCATCAGAACGACCGCCAGAGGCGTAAACCCTCTGTTTTTTTTCATATGTaaa                                                  <  1:1288867/65‑1 (MQ=255)          aGCTGCCTGAGAGCATCAGAACGACCGCCAGAGGCGTAAACCCTCTGTTTTTTTTCATATGTaaa                                                  <  1:862403/65‑1 (MQ=255)           gCTGCCTGAGAGCATCAGAACGACCGCCAGAGGCGTAAACCCTCTGTTTTTTTTCATATGTAAAc                                                 >  1:3009235/1‑65 (MQ=255)           gCTGCCTGAGAGCATCAGAACGACCGCCAGAGGCGTAAACCCTCTGTTTTTTTTCATATGTAAAc                                                 >  1:2003317/1‑65 (MQ=255)           gCTGCCTGAGAGCATCAGAACGACCGCCAGAGGCGTAAACCCTCTGTTTTTTTTCATATGTAAAc                                                 >  1:3532681/1‑65 (MQ=255)           gCTGCCTGAGAGCATCAGAACGACCGCCAGAGGCGTAAACCCTCTGTTTTTTTTCATATGTAAAc                                                 >  1:2961294/1‑65 (MQ=255)           gCTGCCTGAGAGCATCAGAACGACCGCCAGAGGCGTAAACCCTCTGTTTTTTTTCATATGTAAAc                                                 >  1:293042/1‑65 (MQ=255)           gCTGCCTGAGAGCATCAGAACGACCGCCAGAGGCGTAAACCCTCTGTTTTTTTTCATATGTAAAc                                                 >  1:2392592/1‑65 (MQ=255)           gCTGCCTGAGAGCATCAGAACGACCGCCAGAGGCGTAAACCCTCTGTTTTTTTTCATACGTAAAc                                                 >  1:804514/1‑65 (MQ=255)           gCTGCCTGAGAGCATCAGAACGACCGCCAGAGGCGCAAACCCTCTGTTTTTTTTCATATGTAAAc                                                 >  1:2875851/1‑65 (MQ=255)            cTGCCTGAGAGCATCAGAACGACCGCCAGAGGCGTAAACCCTCTGGTTTTTTTCCTATGTAAAcc                                                >  1:2622996/1‑65 (MQ=255)                   agagCATCAGAACGACCGCCAGAGGCGTAAACCCTCTGTTTTTTTTCATATGTAAACCTCGAgtg                                         >  1:1659983/1‑65 (MQ=255)                   agagCATCAGAACGACCGCCAGAGGCGTAAACCCTCTGTTTTTTTTCATATGTAAACCTCGAgtg                                         >  1:1969173/1‑65 (MQ=255)                     agCATCAGAACGACCGCCAGAGGCGTAATCCCTCTGTTTTTTTTCATATGTAAACCTCGAGTGTc                                       >  1:2639587/1‑65 (MQ=255)                      gCATCAGAACGACCGCCAGAGCCGTAAACCCTCTGTTTTTTTTCATATGTAAACCTCGAGTGTcc                                      >  1:2974177/1‑65 (MQ=255)                             aaCGACCGCCAGAGGCGTAAACTCTCCGTTTTTTTTCATATGTACACGCCGGGTGTCCGATTTCa                               >  1:3485883/1‑65 (MQ=255)                              aCGACCGCCCGAGGCGTACACCCTCTGTTTTTTTTCATATGTAAACCTCCAGTGTCCGACTTCaa                              >  1:1742547/1‑65 (MQ=255)                              aCGACCGCCAGAGGCGTAAACCCTCTGTTTTTTTTCATATGTAAACCTCGAGTGTCCGATTTCaa                              >  1:137293/1‑65 (MQ=255)                              aCGACCGCCAGAGGCGTAAACCCTCTGTTTTTTTTCATATGTAAACCTCGAGTGTCCGATTTCaa                              >  1:661880/1‑65 (MQ=255)                                   cGCCAGAGGCGTAAACCCTCTGTTTTTTTTCATATGTAAACCTCGAGTGTCCGATTTCAAATtgg                         >  1:1156210/1‑65 (MQ=255)                                    gCCAGAGGCGTAAACCCTCTGTTTTTTTTCATATGTAAACCTCGAGTGTCCGATTTCAAATtggt                        <  1:2176047/65‑1 (MQ=255)                                        gagGCGTAAACCCTCTGTTTTTTTTCATATGTAAACCTCGAGTGTCCGATTTCAAATtggtcaat                    >  1:1013291/1‑65 (MQ=255)                                        gagGCGTAAACCCTCTGTTTTTTTTCATATGTAAACCTCGAGTGTCCGATTTCAAATtggtcaat                    <  1:2035244/65‑1 (MQ=255)                                        gagGCGTAAACCCTCTGTTTTTTTTCATATGTAAACCTCGAGTGTCCGATTTCAAATtggtcaat                    <  1:90145/65‑1 (MQ=255)                                          ggCGTAAACCCTCTGTTTTTTTTCATATGTAAACCTCGAGTGTCCGATTTCAAATtggtcaatgg                  >  1:3631569/1‑65 (MQ=255)                                            cGTAAACCCTCTGTTTTTTTTCATATGTAAACCTCGAGTGTCCGATTTCGAATtggtcaatggtc                >  1:2374593/1‑65 (MQ=255)                                            cGTAAACCCTCTGTTTTTTTTCATATGTAAACCTCGAGTGTCCGATTTCAAATtggtcaatggtc                >  1:3655907/1‑65 (MQ=255)                                            cGTAAACCCTCTGTTTTTTTTCATATGTAAACCTCGAGTGTCCGATTTCAAATtggtcaatggtc                >  1:2940629/1‑65 (MQ=255)                                            cGTAAACCCTCTGTTTTTTTTCATATGTAAACCTCGAGTGTCCGATTTCAAATtggtcaatggtc                >  1:1899272/1‑65 (MQ=255)                                            cGTAAACCCTCTGTTTTTTTTCATATGTAAACCTCGAGTGTCCGATTTCAAATtggtcaatggtc                >  1:3580482/1‑65 (MQ=255)                                               aaaCCCTCTGTTTTTTTTCATATGTAAACCTCGAGTGTCCGATTTCAAATTGGTCAATGGTCaaa             <  1:267560/65‑1 (MQ=255)                                                 aCCCTCTGTTTTTTTTCATATGTAAACCTCGAGTGTCCGATTTCAAATTGGTCAATGGTCAAAAg           >  1:369914/1‑65 (MQ=255)                                                 aCCCTCTGTTTTTTTTCATATGTAAACCTCGAGTGTCCGATTTCAAATTGGTCAATGGTCAAAAg           >  1:444010/1‑65 (MQ=255)                                                 aCCCTCTGTTTTTTTTCATATGTAAACCTCGAGTGTCCGATTTCAAATTGGTCAATGGTCAAAAg           >  1:538688/1‑65 (MQ=255)                                                 aCCCTCTGTTTTTTTTCATATGTAAACCTCGAGTGTCCGATTTCAAATTGGTCAATGGTCAAAAg           >  1:546347/1‑65 (MQ=255)                                                 aCCCTCTGTTTTTTTTCATATGTAAACCTCGAGTGTCCGATTTCAAATTGGTCAATGGTCAAAAg           >  1:931690/1‑65 (MQ=255)                                                 aCCCTCTGTTTTTTTTCATATGTAAACCTCGAGTGTCCGATTTCAAATTGGTCAATGGTCAAAAg           >  1:3105041/1‑65 (MQ=255)                                                 aCCCTCTGTTTTTTTTCATATGTAAACCTCGAGTGTCCGATTTCAAATTGGTCAATGGTCAAAAg           >  1:3102269/1‑65 (MQ=255)                                                 aCCCTCTGTTTTTTTTCATATGTAAACCTCGAGTGTCCGATTTCAAATTGGTCAATGGTCAAAAg           >  1:2886751/1‑65 (MQ=255)                                                 aCCCTCTGTTTTTTTTCATATGTAAACCTCGAGTGTCCGATTTCAAATTGGTCAATGGTCAAAAg           >  1:1064076/1‑65 (MQ=255)                                                 aCCCTCTGTTTTTTTTCATATGTAAACCTCGAGTGTCCGATTTCAAATTGGTCAATGGTCAAAAg           >  1:1173221/1‑65 (MQ=255)                                                 aCCCTCTGTTTTTTTTCATATGTAAACCTCGAGTGTCCGATTTCAAATTGGTCAATGGTCAAAAg           >  1:2608/1‑65 (MQ=255)                                                 aCCCTCTGTTTTTTTTCATATGTAAACCTCGAGTGTCCGATTTCAAATTGGTCAATGGTCAAAAg           >  1:1339874/1‑65 (MQ=255)                                                 aCCCTCTGTTTTTTTTCATATGTAAACCTCGAGTGTCCGATTTCAAATTGGTCAATGGTCAAAAg           >  1:235541/1‑65 (MQ=255)                                                 aCCCTCTGTTTTTTTTCATATGTAAACCTCGAGTGTCCGATTTCAAATTGGTCAATGGTCAAAAg           >  1:2105567/1‑65 (MQ=255)                                                 aCCCTCTGTTTTTTTTCATATGTAAACCTCGAGTGTCCGATTTCAAATTGGTCAATGGTCAAAAg           >  1:1629798/1‑65 (MQ=255)                                                 aCCCTCTGTTTTTTTTCATATGTAAACCTCGAGTGTCCGATTTCAAATTGGTCAATGGTCAAAAg           >  1:1407729/1‑65 (MQ=255)                                                  cccTCTGTTTTTTTTCATATGTAAACCTCGAGTGTCCGATTTCAAATTGGTCAATGGTCAAAAGt          <  1:2853876/65‑1 (MQ=255)                                                  cccTCTGTTTTTTTTCATATGTAAACCTCGAGTGTCCGATTTCAAATTGGTCAATGGTCAAAAGt          >  1:2320208/1‑65 (MQ=255)                                                   ccTCTGTTTTTTTTCATATGTAAACCTCGAGTGTCTGATTTCAAATTGGTCAATGGTCAAAAGtt         >  1:3306827/1‑65 (MQ=255)                                                    ctctGTTTTTTTTCATATGTAAACCTCGAGTGTCCGATTTCAAATTGGTCAATGGTCAAAAGTta        <  1:32095/65‑1 (MQ=255)                                                    ctctGTTTTTTTTCATATGTAAACCTCGAGTGTCCGATTTCAAATTGGTCAATGGTCAAAAGTta        <  1:853122/65‑1 (MQ=255)                                                    ctctGTTTTTTTTCATATGTAAACCTCGAGTGTCCGATTTCAAATTGGTCAATGGTCAAAAGTta        <  1:2854417/65‑1 (MQ=255)                                                          ttttttTCATATGTAAACCTCGAGTGTCCGATTTCAAATTGGTCAATGGTCAAAAGTTAATAAAc  <  1:2602210/65‑1 (MQ=255)                                                          ttttttTCATATGTAAACCTCGAGTGTCCGATTTCAAATTGGTCAATGGTCAAAAGTTAATAAAc  >  1:2770489/1‑65 (MQ=255)                                                              |                                                             TTAGGGCTAAGCTGCCTGAGAGCATCAGAACGACCGCCAGAGGCGTAAACCCTCTGTTTTTGTTCATATGTAAACCTCGAGTGTCCGATTTCAAATTGGTCAATGGTCAAAAGTTAATAAAC  >  NC_000913/485553‑485674

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑