Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,905,761	G→A	G25D (GGT→GAT)	mntP →	Mn(2(+)) exporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,905,761	0	G	A	100.0%	137.4 / NA	39	G25D (GGT→GAT)	mntP	Mn(2(+)) exporter
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (27/12);  total (27/12)

CTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGGTGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGCCTTAT  >  NC_000913/1905701‑1905812                                                             |                                                    cTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGATGcc                                                 <  1:2811309/65‑1 (MQ=255) cTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTCCATCAATCGGTAAAGATGcc                                                 <  1:1550345/65‑1 (MQ=255)   aCTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGATGCCAc                                               <  1:3371568/65‑1 (MQ=255)   aCTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGATGCCAc                                               >  1:2497090/1‑65 (MQ=255)   aCTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGATGCCAc                                               >  1:521978/1‑65 (MQ=255)    cTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGATGCCAcc                                              <  1:3246549/65‑1 (MQ=255)     tGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGATGCCAccc                                             >  1:557138/1‑65 (MQ=255)     tGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGATGCCAccc                                             >  1:1455427/1‑65 (MQ=255)     tGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGATGCCAccc                                             >  1:2598091/1‑65 (MQ=255)     tGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGATGCCAccc                                             >  1:1663454/1‑65 (MQ=255)     tGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGATGCCAccc                                             >  1:1882549/1‑65 (MQ=255)       ttcttcTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGATGCCACCCTc                                           >  1:882292/1‑65 (MQ=255)        tcttctTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGATGCCACCCTcc                                          >  1:1777205/1‑65 (MQ=255)        tcttctTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGATGCCACCCTcc                                          >  1:499457/1‑65 (MQ=255)        tcttctTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGATGCCACCCTcc                                          >  1:1196306/1‑65 (MQ=255)        tcttctTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGATGCCACCCTcc                                          >  1:959333/1‑65 (MQ=255)        tcttctTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGATGCCACCCTcc                                          >  1:7160/1‑65 (MQ=255)              tGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGATGCCACCCTCCATAAAc                                    <  1:2732098/65‑1 (MQ=255)                      gTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGATGCCACCCTCCATAAACCGAAAttt                            >  1:516628/1‑65 (MQ=255)                       tATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGATGCCACCCTCCATAAACCGAAAtttt                           >  1:3581502/1‑65 (MQ=255)                            cGATGGATGCATTTGCTGCATCAATCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGaa                      >  1:1984931/1‑65 (MQ=255)                            cGATGGATGCATTTGCTGCATCAATCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGaa                      >  1:1853774/1‑65 (MQ=255)                             gatggatgCATTTGCTGCATCAATCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGCAg                     >  1:1571221/1‑65 (MQ=255)                              atggatgCATTTGCTGCATCAATCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGc                    <  1:2640641/65‑1 (MQ=255)                              atggatgCATTTGCTGCATCAATCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGc                    <  1:889318/65‑1 (MQ=255)                              atggatgCATTTGCTGCATCAATCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGc                    <  1:3389201/65‑1 (MQ=255)                                 gatgCATTTGCTGCATCAATCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCAtt                 >  1:747490/1‑65 (MQ=255)                                 gatgCATTTGCTGCATCAATCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCAtt                 <  1:504540/65‑1 (MQ=255)                                 gatgCATTTGCTGCATCAATCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCAtt                 >  1:2939578/1‑65 (MQ=255)                                 gatgCATTTGCTGCATCAATCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCAtt                 >  1:972271/1‑65 (MQ=255)                                  atgCATTTGCTGCATCAATCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTg                >  1:3621707/1‑65 (MQ=255)                                     cATTTGCTGCATCAATCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGa             <  1:3039087/65‑1 (MQ=255)                                       tttGCTGCATCAATCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAAc           >  1:1119961/1‑65 (MQ=255)                                        ttGCTGCATCAATCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAAcc          >  1:3287713/1‑65 (MQ=255)                                        ttGCTGCATCAATCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAAcc          >  1:3135287/1‑65 (MQ=255)                                        ttGCTGCATCAATCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAAcc          >  1:2930542/1‑65 (MQ=255)                                         tgctgcATCAATCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCg         >  1:1059583/1‑65 (MQ=255)                                          gctgcATCAATCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCgg        >  1:322231/1‑65 (MQ=255)                                           ctgcATCAATCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGc       <  1:2980880/65‑1 (MQ=255)                                           ctgcATCAATCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGc       <  1:2534724/65‑1 (MQ=255)                                            tgcATCAATCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGGACCGGcc      >  1:1745593/1‑65 (MQ=255)                                            tgcATCAATCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGcc      >  1:1134668/1‑65 (MQ=255)                                                tCAATCGGTAAAGATGCCCCCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGCCTTAt  <  1:181434/65‑1 (MQ=255)                                                             |                                                    CTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGGTGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGCCTTAT  >  NC_000913/1905701‑1905812

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑