| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,087,406 | 0 | A | C | 58.3% | ‑1.8 / 13.6 | 12 | W285G (TGG→GGG) | yeeY | putative DNA‑binding transcriptional regulator YeeY |
| Reads supporting (aligned to +/- strand): ref base A (5/0); new base C (0/7); total (5/7) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.26e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.39e-02 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TTCCGTCATCAGATTTTGCATAAACACCGGCGATCGCACTATTTGCTAAAATTT‑CATCCCGCCACCAGCCACTGGCAAGTCCTGCGGTCTGTTCGTTCCAGCCAATCCAGACGGGTTCAAAG > NC_000913/2087343‑2087464 | ttCCGTCATCAGATTTTGCATAAACACCGGCGATCGCACTATTTGCTAAAATTT‑CATCCCGccac > 1:3523319/1‑65 (MQ=255) tcaGATTTTGCATAAACACCGGCGATCGCACTATTTGCTAAAATTT‑CATCCCGCCACCAGCCACt > 1:3895151/1‑65 (MQ=255) tcaGATTTTGCATAAACACCGGCGATCGCACTATTTGCTAAAATTT‑CATCCCGCCACCAGCCACt > 1:353287/1‑65 (MQ=255) ggtttttGAAAAACCCCCGGCGTTCCCCTTTTTTGCAAAAATTC‑CACCCCGCCCCCAGCCACTgg < 1:3228009/62‑1 (MQ=255) ataTTTGGCAAAAACGCCGGCGATCGCCTTATTTG‑TAAAATTTCCACCCCGCCGCCAGCCACTgg < 1:2044973/63‑1 (MQ=255) ttGCATAACCCCCGGCGATCGCACTTTTTGCAAAATTTT‑CACCCCCCCCCCAGCCACTGGCAAGt < 1:2652387/65‑1 (MQ=255) ttGCATAAACCCGGGGGTTCGCTTTTTTTGCAAAATTTT‑CCCCCCGCCCCCAGCCACTGGCAAGt < 1:2587584/65‑1 (MQ=255) ttGCATAAACCCGGGCGATCGCATTTTTTGCTAAAATTT‑CACCCCCCCCCCAGCCACTGGCAAGt < 1:127711/65‑1 (MQ=255) ttGCATAAACCCCGGCGATCGCATTTTTTGCAAAAATTT‑CACCCCCCCCCCAGCCACTGGCAAGt < 1:260820/65‑1 (MQ=255) tggCATAAACCCCGGCGATCGCACTATTTGCAAAAATTT‑CACCCCGCCCCCAGCCACTGGCAAGt < 1:3710493/63‑1 (MQ=255) gCAAAAACCCCGGCGATCGCATTTTTTCCTAAAATTC‑CACCCCGCCCCCAGCCACTGGCAAGTcc < 1:3448375/65‑1 (MQ=255) ccATAAACCCGGGCGATCGCATTTTTTGCAAAATTTT‑CACCCCGCCCCCAGCCACTGGCAAGTcc < 1:1841341/64‑1 (MQ=255) aaCACCGGCCATCGCACTTTTTTCTAAAATTA‑CTCCCCGCCACCAGCCACTGGCAAGTCCTGCgg < 1:3847793/65‑1 (MQ=255) gggCGATCGCATTTTTTGCAAAAATTT‑CACCCCCCCCCCAGCCACTGGCAAGTCCTGCGGTCTgt < 1:2797121/64‑1 (MQ=255) cGGCGATCGCTCTATTTGCAAAAATTC‑CACCCCCCCCCCAGCCACTGGCAAGTCCTGCGGTCTgt < 1:2451294/65‑1 (MQ=255) gATCGCACTATTTGCTAAAATTT‑CATCCCGCCACCAGCCACTGGCAAGTCCTGCGGTCTgttcgt > 1:729925/1‑65 (MQ=255) gATCGCACTATTTGCTAAAATTT‑CATCCCGCCACCAGCCACTGGCAAGTCCTGCGGTCTgttcgt > 1:144841/1‑65 (MQ=255) tttCCTAAAGTTT‑CCCCCCCCCCCCAGCCACTGGCAGGTCCTGCGGTCTGTTCGTTCCAGCCAAt < 1:2339111/65‑1 (MQ=255) ccccccccccCAGCCACTGGCAGGTCCTGCGGTCTGTTCGTTCCAGCCAATCCAGACGGGTTCaa < 1:1781753/63‑1 (MQ=255) cccGCCACCAGCCACTGGCCAGTCCTGCGGTCTGTTCGTTCCAGCCAATCCAGACGGGTTCAAag > 1:1515746/1‑65 (MQ=255) | TTCCGTCATCAGATTTTGCATAAACACCGGCGATCGCACTATTTGCTAAAATTT‑CATCCCGCCACCAGCCACTGGCAAGTCCTGCGGTCTGTTCGTTCCAGCCAATCCAGACGGGTTCAAAG > NC_000913/2087343‑2087464 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |