Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,655,2770AC34.5% 46.9 / 14.4 29V209G (GTG→GGG) yhiDinner membrane protein YhiD
Reads supporting (aligned to +/- strand):  ref base A (16/3);  new base C (0/10);  total (16/13)
Fisher's exact test for biased strand distribution p-value = 1.43e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

AATTTCAGGCTTACGGTGAGTCTGGCTACGCTGCCACACAGATTAGCTAATTGAAACGCCTTTCACCCCTGCCATACCTTTTAATAATCGCAACAGGTCTTCTATCGACGTTGTGGCATGAAGCTGGA  >  NC_000913/3655213‑3655340
                                                                |                                                               
aaTTTCAGGCTTACGGTGAGTCTGGCTACGCTGCCACACAGATTAGCTAATTGAAACGCCTTTCa                                                                 <  1:2687413/65‑1 (MQ=255)
 aTTTCAGGCTTACGGTGAGTCTGGCTACGCTGCCACACAGATTAGCTAATTGAAACGCCTTTCAc                                                                >  1:3165395/1‑65 (MQ=255)
  ttccAGGCTTACGGTGAGTCGGGCTCCGCTGCCACACAGATTAGCTAATTGAAACGCCTTTCAcc                                                               <  1:1634120/62‑1 (MQ=255)
     cgggCTTACGGTGAGTCTGGCTACGCTGGCACACAGATTAGCTAATTGAAACGCCTTTCACCCCt                                                            <  1:2314298/63‑1 (MQ=255)
             cGGTGAGTCGGCCTCCCCTCCCACACAGATTAGCTAATTGAAACGCCTTTCCCCCCTGCCATAcc                                                    <  1:545866/65‑1 (MQ=255)
              ggTGAGTCTGGCTACGCTGCCCCACAGATTAGCTAATTGAAACCCCCTTCACCCCTGCCATAccc                                                   >  1:1411681/1‑64 (MQ=255)
                tGAGTCTGGCTCCGCTGCCACACAGATTAGCTAATTAAAACGCCTTTCCCCCCTGCCATACCttt                                                 <  1:1447575/65‑1 (MQ=255)
                tGAGTCTGGCTACGCTTCCACACAGATTAGCTAATTGAAACGCCTTTCACCCCTGCCATACCttt                                                 >  1:991558/1‑65 (MQ=255)
                    tCTGGCTACGCTGCCACACAGATTAGCTAATTGAAACGCCTTTCACCCCTGCCATACCTTTtaat                                             >  1:1679235/1‑65 (MQ=255)
                    tCTGGCTACGCTGCCACACAGATTAGCTAATTGAAACGCCTTTCACCCCTGCCATACCTTTtaat                                             >  1:3566443/1‑65 (MQ=255)
                    tCTGGCTACGCTGCCACACAGATTAGCTAATTGAAACGCCTTTCACCCCTGCCATACCTTTtaat                                             >  1:2903122/1‑65 (MQ=255)
                        gCTACGCTGCCACACAGATTAGCTAATTGAAACGCCTTTCACCCCTGCCATACCTTTTAATAATc                                         >  1:1468311/1‑65 (MQ=255)
                         cTACCCTGCCACACAGATTAGCTAATTGAACCGCCTTTCCCCCCTGCCATACCTTTTAATAATCg                                        <  1:1617142/65‑1 (MQ=255)
                              cTGCCACACAGATTAGCTAATTGAAACGCCTTTCACCCCTGCCATACCTTTTAATAATCGCAACa                                   >  1:1581779/1‑65 (MQ=255)
                              cTGCCACACAGATTAGCTAATTGAAACGCCTTTCACCCCTGCCATACCTTTTAATAATCGCAACa                                   >  1:644221/1‑65 (MQ=255)
                              cTGCCACACAGATTAGCTAATTGAAACGCCTTTCACCCCTGCCATACCTTTTAATAATCGCAACa                                   >  1:2599292/1‑65 (MQ=255)
                              cTGCCACACAGATTAGCTAATTAAACCCCCTTTCCCCCCTGCCATACCTTTTAATAATCGCAACa                                   <  1:853663/65‑1 (MQ=255)
                                 cctcactGATTACCTAATTAAACCGCTTTTCACCCCTGCCATACCTTTTAATAATCGCAACAGGt                                <  1:3895459/62‑1 (MQ=255)
                                 ccACACAGATTAGCTAATTGAACCGCCTTTCCCCCCTGCCATACCTTTTAATAATCGCAACAGGt                                <  1:1262192/65‑1 (MQ=255)
                                 ccACACAGATTAGCTAATTGAAACGCCTTTCGCCCCTGCCATACCTTTTAATAATCGCAACAGGt                                <  1:2837307/65‑1 (MQ=255)
                                 ccACACAGATTAGCTAATTGAAACGCCTTTCCCCCCTGCCATACCTTTTAATAATCGCAACAGGt                                <  1:1502214/65‑1 (MQ=255)
                                 ccACACAGATTAGCTAATTGAAACGCCTTTCCCCCCTGCCATACCTTTTAATAATCGCAACAGGt                                <  1:3986332/65‑1 (MQ=255)
                                 ccACACAGATTAGCTAATTGAAACGCCTTTCCCCCCTGCCATACCTTTTAATAATCGCAACAGGt                                <  1:638491/65‑1 (MQ=255)
                                 ccACACAGATTAGCTAATTAAACCCCCTTTCCCCCCTGCCATACCTTTTAATAATCGCAACAGGt                                <  1:3867578/65‑1 (MQ=255)
                                 caacaaaGATTAGCTAATTGAAACGCCTTTCACCCCTGCCATACCTTTTAATAATCGCAACAGGt                                <  1:785402/63‑1 (MQ=255)
                                   ccacaGATTAGCTAATTGAACCGCCTTTCCCCCCTGCCATACCTTTTAATAATCGCAACAGGtct                              <  1:2961028/64‑1 (MQ=255)
                                     aaaGATTAGCTAATTGAACCCCCTTCCCCCCCTGCCATACCTTTTAATAATCGCAACAGGtcttc                            <  1:3319374/63‑1 (MQ=255)
                                      caGATTACCTAATTGAAGCGCCTTTCCCCCCTGCCATACCTTTTAATAATCGCAACAGGtcttct                           <  1:3698741/65‑1 (MQ=255)
                                       aGATTAGCTAATTGAAACGCCTTTCACCCCTGCCATACCTTTTAATAATCGCAACAGGTCTTCTa                          >  1:295574/1‑65 (MQ=255)
                                       aGATTAGCTAATTGAAACGCCTTTCACCCCTGCCATACCTTTTAATAATCGCAACAGGTCTTCTa                          >  1:2528551/1‑65 (MQ=255)
                                        gATTAGCTAATTGAAACCCCTTTCCCCCCTGCCATACCTTTTAATAATCGCAACAGGTCTTCTAt                         <  1:3892020/65‑1 (MQ=255)
                                            aGCTAATTGAAGCCCCTTTCCCCCCTGCCATACCTTTTAATAATCGCAACAGGTCTTCTATCGAc                     <  1:592620/65‑1 (MQ=255)
                                            aGCTAATTGAACCGCCTTCCCCCCCTGCCATACCTTTTAATAATCGCAACAGGTCTTCTATCGAc                     <  1:3311529/65‑1 (MQ=255)
                                            aGCTAATGAAACCGCCTTTCCCCCCTGCCATACCTTTTAATAATCGCAACAGGTCTTCTATCGAc                     <  1:3746016/65‑1 (MQ=255)
                                               tAATTGAACCGCCTTTCCCCCCTGCCATACCTTTTAATAATCGCAACAGGTCTTCTATCGACGtt                  <  1:3736052/65‑1 (MQ=255)
                                               tAATTGAAACGCCTTTCACCCCTGCCATACCTTTTAATAATCGCAACAGGTCTTCTATCGACGtt                  >  1:2685296/1‑65 (MQ=255)
                                               tAATTGAAACGCCTTTCACCCCTGCCATACCTTTTAATAATCGCAACAGGTCTTCTATCGACGtt                  >  1:3687928/1‑65 (MQ=255)
                                               tAATTGAAACGCCTTTCACCCCTGCCATACCTTTTAATAATCGCAACAGGTCTTCTATCGACGtt                  >  1:2176506/1‑65 (MQ=255)
                                                   tGAAACGCCTTTCACCCCTGCCATACCTTTTAATAATCGCAACAGGTCTTCTATCGACGTTGTgg              >  1:3576948/1‑65 (MQ=255)
                                                   tGAAACGCCTTTCACCCCTGCCATACCTTTTAATAATCGCAACAGGTCTTCTATCGACGTTGTgg              >  1:1181874/1‑65 (MQ=255)
                                                       aCGCCTTTCACCCCTGCCATACCTTTTAATAATCGCAACAGGTCTTCTATCGACGTTGTGGCTTg          >  1:3658687/1‑65 (MQ=255)
                                                         gcttttCCCCCCTGCCATACCTTTTAATAATCGCAACAGGTCTTCTATCGACGTTGTGGCATGaa        <  1:2886877/62‑1 (MQ=255)
                                                         gCCTTTCCCCCCTGCCATACCTTTTAATAATCGCAACAGGTCTTCTATCGACGTTGTGGCATGaa        <  1:644022/65‑1 (MQ=255)
                                                         gCCTTTCCCCCCTGCCATACCTTTTAATAATCGCAACAGGTCTTCTATCGACGTTGTGGCATGaa        <  1:297314/65‑1 (MQ=255)
                                                         gCCTTTCACCCCTGCCATACCTTTTAATAATCGCAACAGGTCTTCTATCGACGTTGTGGCATGaa        >  1:98464/1‑65 (MQ=255)
                                                         cccTTTCCCCCCTGCCATACCTTTTAATAATCGCAACAGGTCTTCTATCGACGTTGTGGCATGaa        <  1:33381/64‑1 (MQ=255)
                                                              tCACCCCTGCCATACCTTTTAATAATCGCAACAGGTCTTCTATCGACGTTGTGGCATGAAGCTgg   <  1:1192209/65‑1 (MQ=255)
                                                               cACCCCTGCCATATCTTTTAATAATCGCAACAGGTCTTCTATCGACGTTGTGGCATGAAGCTGGa  <  1:1878452/65‑1 (MQ=255)
                                                                |                                                               
AATTTCAGGCTTACGGTGAGTCTGGCTACGCTGCCACACAGATTAGCTAATTGAAACGCCTTTCACCCCTGCCATACCTTTTAATAATCGCAACAGGTCTTCTATCGACGTTGTGGCATGAAGCTGGA  >  NC_000913/3655213‑3655340

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: