Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,390,754	G→T	A252A (GCC→GCA)	rsgA ←	ribosome small subunit‑dependent GTPase A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,390,754	0	G	T	100.0%	120.7 / NA	33	A252A (GCC→GCA)	rsgA	ribosome small subunit‑dependent GTPase A
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (25/8);  total (25/8)

ACTCACGCACTCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGTGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGATCTC  >  NC_000913/4390693‑4390813                                                              |                                                            aCTCACGCACTCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAgctgcg                                                          >  1:685937/1‑65 (MQ=255)   tCACGCACTCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGt                                                        <  1:3521614/65‑1 (MQ=255)   ccACGCACTGCTGGGGAGTCAATCACATCCCCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGt                                                        <  1:1224878/64‑1 (MQ=255)           tCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGgtgt                                                <  1:393562/65‑1 (MQ=255)                   aGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGGTGTGCTGAccc                                        >  1:1009490/1‑64 (MQ=255)                   aGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGGTGTGCTGACCg                                        >  1:1453210/1‑65 (MQ=255)                   aGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGGTGTGCTGACCg                                        >  1:1005183/1‑65 (MQ=255)                   aGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGGTGTGCTGACCg                                        >  1:1597771/1‑65 (MQ=255)                   aGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGGTGTGCTGACCg                                        >  1:901615/1‑65 (MQ=255)                   aGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGGTGTGCTGACCg                                        >  1:2076932/1‑65 (MQ=255)                   aGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGGTGTGCTGACCg                                        >  1:2353389/1‑65 (MQ=255)                   aGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGGCGTGCTGACCg                                        >  1:764866/1‑65 (MQ=255)                   aGTCAATCACATCACCGCCGTGCGGGAAGGGATACAGCCGAGCTGCGGTCGTGGTGTGCTGACCg                                        >  1:91289/1‑65 (MQ=255)                   aGTCAATCACATCACCGCCGTGCGGGAAGCGATACAGCCGAGCTGCGGTCGTGGCGTGCTGACCg                                        >  1:1961605/1‑65 (MQ=255)                     tCAATCCCATCCCCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGGTGTGCTGACCGAg                                      <  1:2952981/65‑1 (MQ=255)                           acatcacCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGGTGTGCTGACCGAGCCCCGa                                >  1:2675611/1‑65 (MQ=255)                              tcacCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGGTGTGCTGACCGAGCCCCGAGtt                             >  1:3242032/1‑65 (MQ=255)                              tcacCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGGTGTGCTGACCGAGCCCCGAGtt                             >  1:3336093/1‑65 (MQ=255)                               cacCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGGTGTGCTGACCGAGCCCCGAGTTg                            <  1:3660230/65‑1 (MQ=255)                                 ccgccgTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGGTGTGCTGACCGAGCCCCGAGTTGTc                          <  1:1577508/65‑1 (MQ=255)                                   gccgTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGGTGTGCTGACCGAGCCCCGAGTTGTCag                        >  1:2628514/1‑65 (MQ=255)                                   gccgTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGGTGTGCTGACCGAGCCCCGAGTTGTCag                        >  1:1839358/1‑65 (MQ=255)                                   gccgTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGGTGTGCTGACCGAGCCCCGAGTTGTCag                        >  1:479736/1‑65 (MQ=255)                                   gccgTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGGTGTGCTGACCGAGCCCCGAGTTGTCag                        >  1:2532924/1‑65 (MQ=255)                                          gggAATTGATACAGCCGAGCTGCGGTCGTGGTGTGCTGACCGAGCCCCGAGTTGTCAGAGATATc                 <  1:3671769/65‑1 (MQ=255)                                          gggAAGTGATACAGCCGAGCTGCGGTCGTGGTGTGCTGACCGAGCCCCGAGTTGTCAGAGATATc                 >  1:3720116/1‑65 (MQ=255)                                              aGTGATACAGCCGAGCTGCGGTCGTGGTGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTg             >  1:3808110/1‑65 (MQ=255)                                              aGTGATACAGCCGAGCTGCGGTCGTGGTGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTg             >  1:401668/1‑65 (MQ=255)                                              aGTGATACAGCCGAGCTGCGGTCGTGGTGTGCTGACCGAGCCCCGAGGTGTCAGAGCTATCGTTg             >  1:3063571/1‑65 (MQ=255)                                              aGTGATACAGCCGAGCTGCGGTCGTGGGGTGCTGACCGAGCCCCGAGTTGTCAGAGACATCGTTg             >  1:2560047/1‑65 (MQ=255)                                               gTGAGACAGCCGAGCTGCGGTCGTGGTGTGCTGCCGGAGCCCCGAGTTGTCAGAGATATCGTTgg            <  1:2879796/65‑1 (MQ=255)                                                  aTACAGCCGAGCTGCGGTCGTGGTGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCa         >  1:1964542/1‑65 (MQ=255)                                                  aTACAGCCGAGCTGCGGTCGTGGTGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCa         >  1:3468558/1‑65 (MQ=255)                                                      aGCCGAGCTGCGGTCGTGGTGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGAt     >  1:1606356/1‑65 (MQ=255)                                                       gCCGAGCTGCGGTCGTGGTGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGAtc    >  1:2156249/1‑65 (MQ=255)                                                       gCCGAGCTGCGGTCGTGGTGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGAtc    >  1:593727/1‑65 (MQ=255)                                                       gCCGAGCTGCGGTCGTGGTGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGAtc    >  1:650518/1‑65 (MQ=255)                                                         cGAGCTGCGGTCGTGGTGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGAtctc  <  1:487934/65‑1 (MQ=255)                                                              |                                                            ACTCACGCACTCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGTGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGATCTC  >  NC_000913/4390693‑4390813

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑