Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	49,905	T→G	L28R (CTC→CGC)	folA →	dihydrofolate reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	49,905	0	T	G	100.0%	123.8 / NA	36	L28R (CTC→CGC)	folA	dihydrofolate reductase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (29/7);  total (29/7)

GTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGG  >  NC_000913/49843‑49964                                                               |                                                            tttAGCGGTAGACCGCGTTATCGGCATGGAAACCCCCATGCTGTGGAACCTGCCTGCCGATcgcg                                                           <  1:1004693/64‑1 (MQ=255) gTTAGCGGTAGATCGCGTTATCGGCATGGAAACCGCCATGCTGTGGACCCTGCCTGCCGATcgcg                                                           <  1:3866678/65‑1 (MQ=255) gTTAGCGGTAGATCGCGTTAGCGGCATGGAAAACGCCTTGCTGTGGAACCTGCCTGCCGATcgcg                                                           <  1:796317/65‑1 (MQ=255)       gctAGACCGGGTTAGGGGAATGGAAAACGCGTTGCGGTGGAGCCTGCCTGCCGATCGCGCCTGGt                                                     <  1:2693585/63‑1 (MQ=255)        gTAGATCGCGTTATCGGCATGGAAAACGCCATGCTGTGGAACCTGCCTGCCGATCGCGCCTGGtt                                                    >  1:817160/1‑65 (MQ=255)          aGATCGCGTTATCGGCATGGAAAACGCCATGCTGTGGAACCTGCCTGCCGATCGCGCCTGGTTTa                                                  >  1:1223018/1‑65 (MQ=255)            aTCGCGTTATCGGCATGGAAAACGCCATGCTGTGGAACCTGCCTGCCGATCGCGCCTGGTTTaaa                                                >  1:2104965/1‑65 (MQ=255)            aTCGCGTTATCGGCATGGAAAACGCCATGCTGTGGAACCTGCCTGCCGATCGCGCCTGGTTTaaa                                                >  1:3109264/1‑65 (MQ=255)            aTCGCGTTATCGGCATGGAAAACGCCATGCTGTGGAACCTGCCTGCCGATCGCGCCTGGTTTaaa                                                >  1:2865174/1‑65 (MQ=255)            aTCGCGTTATCGGCATGGAAAACGCCATGCTGTGGAACCTGCCTGCCGATCGCGCCTGGTTTaaa                                                >  1:285277/1‑65 (MQ=255)            aTCGCGTTATCGGCATGGAAAACGCCATGCTGTGGAACCTGCCTGCCGATCGCGCCTGGTTTaaa                                                >  1:487093/1‑65 (MQ=255)              cgcgTTATCGGCATGGAAAACGCCATGCTGTGGAACCTGCCTGCCGATCGCGCCTGGTTTAAACg                                              >  1:3237357/1‑65 (MQ=255)                 gTTATCGGCATGGAAAACGCCATGCTGTGGAACCTGCCTGCCGATCGCGCCTGGTTTAAACgtct                                           >  1:2375853/1‑62 (MQ=255)                    aTCGGCATGGAAAACGCCATGCTGTGGACCCTGCCTGCCGATCGCGCCTGGTTTAAACGCAacac                                        <  1:461149/65‑1 (MQ=255)                    aTCGGCATGGAAAACGCCATGCTGTGGAACCTGCCTGCCGATCGCGCCTGGTTTAAACGCAacac                                        <  1:2237580/65‑1 (MQ=255)                     tCGGCATGGAAAACGCCATGCTGTGGAACCTGCCTGCCGATCGCGCCTGGTTTAAACGCAACAcc                                       <  1:3231433/65‑1 (MQ=255)                       ggCATGGAAAACGCCATGCTGTGGAACCTGCCTGCCGATCGCGCCTGGTTTAAACGCAACACCtt                                     >  1:3378340/1‑65 (MQ=255)                         cATGGAAACCGCCATGCTTTGGAACCTGCCTGCCGATCGCGCCTGGTTTAAACGCAACACCTtaa                                   <  1:13646/65‑1 (MQ=255)                         cATGGAAAACGCCATGCTGTGGACCCTGCCTGCCGATCGCGCCTGGTTTAAACGCAACACCTtaa                                   <  1:2793909/65‑1 (MQ=255)                              aaaaCGCCATGCTGTGGAACCTGCCTGCCGATCGCGCCTGGTTTAAACGCAAACCCTtcaataac                              >  1:3683377/1‑64 (MQ=255)                               aaccGCCATGCTGTGGAACCTGCCTGCCGATCGCGCCTGGTTTAAACGCAACACCTTAAATAAAc                             <  1:1322103/62‑1 (MQ=255)                                   gCCATGCTGTGGAACCTGCCTGCCGATCGCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGt                         >  1:1613565/1‑65 (MQ=255)                                   gCCATGCTGTGGAACCTGCCTGCCGATCGCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGt                         >  1:3185976/1‑65 (MQ=255)                                    ccATGCTGTGGAACCTGCCTGCCGATCGCCCCTGGTTTAAACGCAACACCTTACATAAACTCGTg                        >  1:1795755/1‑65 (MQ=255)                                        gctgTGGAACCTGCCTGCCGATCGCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTa                    >  1:3766411/4‑65 (MQ=255)                                        gctgTGGAACCTGCCTGCCGATCGCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTa                    >  1:173269/4‑65 (MQ=255)                                         ctgTGGAACCTGCCTGCCGATCGCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTAt                   >  1:1523883/3‑65 (MQ=255)                                               aaCCTGCCTGCCGATCGCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGgccg             >  1:2785181/1‑65 (MQ=255)                                               aaCCTGCCTGCCGATCGCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGgccg             >  1:3202017/1‑65 (MQ=255)                                               aaCCTGCCTGCCGATCGCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGgccg             >  1:222320/1‑65 (MQ=255)                                               aaCCTGCCTGCCGATCGCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGCGATTATGGgccg             >  1:2533160/1‑65 (MQ=255)                                               aaCCTGCCTGCCGATCGCGCCTGGTTTAAACACAACACCTTAAATAAACCCGTGATTATGGgccg             >  1:951267/1‑65 (MQ=255)                                                aCCTGCCTGCCGATCGCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGgccgc            >  1:3949756/1‑65 (MQ=255)                                                aCCTGCCTGCCGATCGCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGgccgc            >  1:1686477/1‑65 (MQ=255)                                                aCCTGCATGCCGATCGCGCCTGGGTTAAACGCAACACCTTAAATAAACCCGTGATTATGGgccgc            >  1:3008159/1‑65 (MQ=255)                                                 cctgcctgCCGATCGCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGgccgcc           >  1:3228689/1‑65 (MQ=255)                                                  ctgcctgcCGATCGCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCa          >  1:1974720/1‑65 (MQ=255)                                                  ctgcctgcCGATCGCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCa          >  1:3901814/1‑65 (MQ=255)                                                  ctgcctgcCGATCGCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCa          >  1:1571629/1‑65 (MQ=255)                                                      cggccGATCGCGCTTGTTTAAAACGCAACCCTAAAAATAACCCCGTGATTATGGGCCGCCATAcc      <  1:1829757/63‑1 (MQ=255)                                                        gccGATCGCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTg    >  1:2605879/1‑65 (MQ=255)                                                        gccGATCGCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTg    >  1:1896092/1‑65 (MQ=255)                                                          cGATCGCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTggg  >  1:2075513/1‑65 (MQ=255)                                                               |                                                            GTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGG  >  NC_000913/49843‑49964

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑