Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	481,400	C→T	A1002T (GCA→ACA)	acrB ←	multidrug efflux pump RND permease AcrB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	481,400	0	C	T	100.0%	112.3 / NA	33	A1002T (GCA→ACA)	acrB	multidrug efflux pump RND permease AcrB
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (22/11);  total (22/11)

AACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAACCAGCGGCATAACGCCGAGGATAAAC  >  NC_000913/481338‑481461                                                               |                                                              aaCGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTgtgt                                                             <  1:3682227/65‑1 (MQ=255)   cGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGGGTTc                                                           >  1:3826686/1‑65 (MQ=255)       gaagaTTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGTGTTCTgcg                                                       >  1:3552522/1‑65 (MQ=255)           aTTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGTGTTCTGCGCGcc                                                   <  1:3092953/65‑1 (MQ=255)              gCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGTGTTCTGCGCGCCGGa                                                >  1:2824876/1‑65 (MQ=255)              gCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGTGTTCTGCGCGCCGGa                                                >  1:1582326/1‑65 (MQ=255)              gCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGTGTTCTGCGCGCCGGa                                                >  1:3578124/1‑65 (MQ=255)                  gTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGTGTTCTGCGCGCCGGAACCa                                            >  1:2600485/1‑65 (MQ=255)                    aCCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGTGTTCTGCGCGCCGGAACCAGc                                          >  1:2887995/1‑65 (MQ=255)                    aCCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGTGTTCTGCGCGCCGGAACCAGc                                          >  1:3085799/1‑65 (MQ=255)                     ccGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGTGTTCTGCGCGCCGGAACCAGCa                                         <  1:2900995/65‑1 (MQ=255)                     ccGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGTGTTCTGCGCGCCGGAACCAGCa                                         <  1:2943605/65‑1 (MQ=255)                     ccGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGTGTTCTGCGCGCCGGAACCAGCa                                         >  1:1328000/1‑65 (MQ=255)                      cGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGTGTTCTGCGCGCCGGAACCAGccc                                        >  1:2931473/1‑63 (MQ=255)                       gTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGTGTTCTGCGCGCCGGAACCAGCAcc                                       <  1:1024046/65‑1 (MQ=255)                             gTCACCATCCCGCCCATTACACCGGTACCTACTGTGTTCTGCGCGCCGGAACCAGCACCAGTACt                                 <  1:1194697/65‑1 (MQ=255)                                aCCATCCCGCCCATTACACCGGTACCTACTGTGTTCTGCGCGCCGGAACCAGCACCAGTACTGAt                              >  1:84519/1‑65 (MQ=255)                                   aTCCCGCCCATTACACCGGTACCTACTGTGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAAc                           >  1:2910116/1‑65 (MQ=255)                                    tCCCGCCCATTACACCGGTACCTACTGTGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAAcc                          <  1:84848/65‑1 (MQ=255)                                        gCCCATTACACCGGTACCTACTGTGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAACCAGCg                      >  1:3499856/1‑65 (MQ=255)                                          ccATTACACCGGTACCTACTGTGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAACCAGCGGc                    <  1:1084628/65‑1 (MQ=255)                                          ccATTACACCGGTACCTACTGTGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAACCAGCGGc                    <  1:1186481/65‑1 (MQ=255)                                          ccATTACACCGGTACCTACTGTGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAACCAGCGGc                    <  1:495138/65‑1 (MQ=255)                                          ccATTACACCGGTACCTACTGTGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAACCAGCGGc                    <  1:1766288/65‑1 (MQ=255)                                          ccATCACCCCGGTACCTACTGTGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAACCAGCGGc                    <  1:1718814/65‑1 (MQ=18)                                                cacCGGTACCTACTGTGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAACCAGCGGCATAACg              >  1:2615030/1‑65 (MQ=255)                                                cacCGGTACCTACTGTGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAACCAGCGGCATAACg              >  1:3257832/1‑65 (MQ=255)                                                  ccGGTACCTACTGTGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAACCAGCGGCATAACGcc            >  1:553164/1‑65 (MQ=255)                                                  ccGGTACCTACTGTGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAACCAGCGGCATAACGcc            >  1:739310/1‑65 (MQ=255)                                                    ggTACCTACTGTGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAACCAGCGGCATAGCGCAGa          >  1:3899268/1‑65 (MQ=21)                                                    ggTACCTACTGTGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAACCAGCGGCATAACGCCGa          >  1:2395501/1‑65 (MQ=255)                                                      tACCTACTGTGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAACCAGCGGCATAACGCCGAgg        <  1:3612426/65‑1 (MQ=255)                                                      tACCTACTGTGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAACCAGCGGCATAACGCCGAgg        <  1:3056127/65‑1 (MQ=255)                                                       aCCTACTGTGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAACCAGCGGCATAACGCCGAGGa       >  1:1810091/1‑65 (MQ=255)                                                       aCCTACTGTGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAACCAGCGGCATAACGCCGAGGa       >  1:489589/1‑65 (MQ=255)                                                        ccTACTGTGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAACCAGCGGCATAACGCCGAGGAt      <  1:3967239/65‑1 (MQ=255)                                                         cTACTGTGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAACCAGCGGCATAACGCCGAGGATa     >  1:61875/1‑65 (MQ=255)                                                         cTACTGTGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAACCAGCGGCATAACGCCGAGGATa     >  1:2670375/1‑65 (MQ=255)                                                           aCTGTGTTCTGCGCGCCGGAACCAGCACCCGTACTGATAACCAGCGGCATAACGCCGAGGATaaa   >  1:2916763/1‑65 (MQ=255)                                                           aCTGTGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAACCAGCGGCATAACGCCGAGGATaaa   >  1:3643364/1‑65 (MQ=255)                                                           aCTGTGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAACCAGCGGCATAACGCCGAGGATaaa   >  1:795322/1‑65 (MQ=255)                                                           aCTGTGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAACCAGCGGCATAACGCCGAGGATaaa   >  1:2843033/1‑65 (MQ=255)                                                           aCTGTGTTCTGCGCGCCGGAACCAGCACCAGAACTGATAACCAGCGGCATAGCGCGGAGGACaaa   >  1:288507/1‑65 (MQ=21)                                                            cTGTGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAACCAGCGGCATAACGCCGAGGATAAAc  <  1:2626644/65‑1 (MQ=255)                                                               |                                                              AACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAACCAGCGGCATAACGCCGAGGATAAAC  >  NC_000913/481338‑481461

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑