| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 485,614 | G→T | N2K (AAC→AAA) | acrA ← | multidrug efflux pump membrane fusion lipoprotein AcrA |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 485,614 | 0 | G | T | 98.6% | 256.9 / ‑2.8 | 73 | coding (6/1194 nt) | acrA | multidrug efflux pump membrane fusion lipoprotein AcrA |
| Reads supporting (aligned to +/- strand): ref base G (0/0); major base T (55/17); minor base . (0/1); total (55/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.47e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.32e-01 | |||||||||||
GTTAGGGCTAAGCTGCCTGAGAGCATCAGAACGACCGCCAGAGGCGTAAACCCTCTG‑TTTTTGTTCATATGTAAACCTCGAGTGTCCGATTTCAAATTGGTCAATGGTCAAAAGTTAATAAAC > NC_000913/485552‑485674 | gTTAGGGCTAAGCTGCCTGAGAGCATCAGAACGACCGCCAGAGGCGTAAACCCTCTG‑tttttttt < 1:2384618/65‑1 (MQ=255) ttAGGGCTAAGCTGCCTGAGAGCATCAGAACGACCGCCAGAGGCGTAAACCCTCTG‑TTTTTTTTc > 1:726457/1‑65 (MQ=255) gggCTAAGCTGCCTGAGAGCATCAGAACGACCGCCAGAGGCGTAAACCCTCTG‑TTTTTTTTCata < 1:1043241/65‑1 (MQ=255) ggCTAAGCTGCCTGAGGGCATCAGAACGACCGCCAGAGGCGTAAACCCTCTG‑TTTTTTTTCatat > 1:607036/1‑65 (MQ=255) ggCTAAGCTGCCTGAGAGCATCAGAACGACCGCCAGAGGCGTAAACCCTCTG‑TTTTTTTTCatat > 1:335319/1‑65 (MQ=255) ggCTAAGCTGCCTGAGAGCATCAGAACGACCGCCAGAGGCGTAAACCCTCTG‑TTTTTTTTCatat > 1:3242143/1‑65 (MQ=255) ggCTAAGCTGCCTGAGAGCATCAGAACGACCGCCAGAGGCGTAAACCCTCTG‑TTTTTTTTCatat < 1:464574/65‑1 (MQ=255) ggCTAAGCTGCCTGAGAGCATCAGAACGACCACCAGAGGCGTAAACCCTCTG‑TTTTTTTTCatat > 1:3341834/1‑65 (MQ=255) cTAAGCTGCCTGAGAGCATCAGAACGACCGCCAGAGGCGTAAACCCTCTG‑TTTTTTTTCATATGt > 1:423717/1‑65 (MQ=255) cTAAGCTGCCTGAGAGCATCAGAACGACCGCCAGAGGCGTAAACCCTCTG‑TTTTTTTTCATATGt > 1:1862953/1‑65 (MQ=255) aaGCTGCCTGAGAGCATCAGAACGACCGCCAGAGGCGTAAACCCTCTG‑TTTTT‑TTCATATGTaaa < 1:3873217/65‑1 (MQ=255) aGCTGCCTGAGAGCATCAGAACGACCGCCAGAGGCGTAAACCCTCTG‑TTTTTTTTCATATGTaaa < 1:2965940/65‑1 (MQ=255) aGCTGCCTGAGAGCATCAGAACGACCGCCAGAGGCGTAAACCCTCTG‑TTTTTTTTCATATGTaaa < 1:3880955/65‑1 (MQ=255) gCTGCCTGAGAGCATCAGAACGACCGCCAGAGGCGTAAACCCTCTG‑TTTTTTTTCATATGTacac > 1:2403983/1‑65 (MQ=255) gCTGCCTGAGAGCATCAGAACGACCGCCAGAGGCGTAAACCCTCTG‑TTTTTTTTCATATGTAAAc > 1:643300/1‑65 (MQ=255) gCTGCCTGAGAGCATCAGAACGACCGCCAGAGGCGTAAACCCTCTG‑TTTTTTTTCATATGTAAAc > 1:1107044/1‑65 (MQ=255) gCTGCCTGAGAGCATCAGAACGACCGCCAGAGGCGTAAACCCTCTG‑TTTTTTTTCATATGTAAAc > 1:1975004/1‑65 (MQ=255) gCTGCCTGAGAGCATCAGAACGACCGCCAGAGGCGTAAACCCTCTG‑TTTTTTTTCATATGTAAAc > 1:1924692/1‑65 (MQ=255) gCTGCCTGAGAGCATCAGAACGACCGCCAGAGGCGTAAACCCTCTG‑TTTTTTTTCATATGTAAAc > 1:3189791/1‑65 (MQ=255) gCTGCCTGAGAGCATCAGAACGACCGCCAGAGGCGTAAACCCTCTG‑TTTTTTTTCATATGTAAAc > 1:2877814/1‑65 (MQ=255) cTGCCTGAGAGCATCAGAACGACCGCCAGAGGCGTAAACCCTCTG‑TTTTTTTTCATATGTAAAcc > 1:2875936/1‑65 (MQ=255) ccTGAGAGCATCAGAACGACCGCCAGAGGCGTAAACCCTCTG‑TTTTTTTTCATATGTAAACCTCg < 1:1693897/65‑1 (MQ=255) cTGAGAGCATCAGAACGACCGCCAGAGGCGTAAACCCTCTG‑TTTTTTTTCATATGTAAACCTCGa > 1:2022171/1‑65 (MQ=255) tGAGAGCATCAGAACGACCGCCAGAGGCGTAAACCCTCTG‑TTTTTTTTCATATGTCAACCTCGAg > 1:1842660/1‑65 (MQ=255) tGAGAGCATCAGAACGACCGCCAGAGGCGTAAACCCTCTG‑TTTTTTTTCATATGTAAACCTCGAg > 1:3808725/1‑65 (MQ=255) agagCATCAGAACGACCGCCAGAGGCGTAAACCCTCTG‑TTTTTTTTCATATGTAAACCTCGAgtg > 1:1954030/1‑65 (MQ=255) cgCATCAGAACGACCGCCAGAGGCGTAAACCCTCTG‑TTTTTTTTCATATGTAAACCTCGAGTGTc > 1:1842609/2‑65 (MQ=255) agCATCAGAACGACCGCCAGAGGCGTAAACCCTCTG‑TTTTTTTTCATATGTAAACCTCGAGTGTc > 1:874504/1‑65 (MQ=255) agCATCAGAACGACCGCCAGAGGCGTAAACCCTCTG‑TTTTTTTTCATATGTAAACCTCGAGTGTc > 1:3060322/1‑65 (MQ=255) agCATCAGAACGACCGCCAGAGGCGTAAACCCTCTG‑TTTTTTTTCATATGTAAACCTCGAGTGTc > 1:3458874/1‑65 (MQ=255) agCATCAGAACGACCGCCAGAGGCGGGAACCCTCTGTTTTTTTTTCATATATAAAACTCGCgttc > 1:2147598/1‑63 (MQ=255) gCATCAGAACGACCGCCAGAGGCGTAAACCCTCTG‑TTTTTTTTCATATGTAAACCTCGAGTGTcc > 1:1968156/1‑65 (MQ=255) tCAGAACGACCGCCAGAGGCGTAAACCCTCTG‑TTTTTTTTCATATGTAAACCTCGAGTGTCCGAt < 1:2601977/65‑1 (MQ=255) tCAGAACGACCGCCAGAGGCGTAAACCCTCTG‑TTTTTTTTCATATGTAAACCTCGAGTGTCCGAt < 1:2143939/65‑1 (MQ=255) tCAGAACGACCGCCAGAGGCGTAAACCCTCTG‑TTTTTTTTCATATGTAAACCTCGAGTGTCCGAt < 1:1040895/65‑1 (MQ=255) tCAGAACGACCGCCAGAGGCGTAAACCCTCTG‑TTTTTTTTCATATGTAAACCTCGAGTGTCCGAt > 1:429448/1‑65 (MQ=255) tCAGAACGACCGCCAGAGGCGTAAACCCTCTG‑TTTTTTTTCATATGTAAACCTCGAGTGTCCGAt < 1:907543/65‑1 (MQ=255) tCAGAACGACCGCCAGAGGCGTAAACCCTCTG‑TTTTTTTTCATATGTAAACCTCGAGTGTCCGAt < 1:1268830/65‑1 (MQ=255) tCAAAACGGCCCCCAGGGGCGTAAACCCTCTG‑TTTTTTTTCATATGTAAACCGCGAGTGTCCGAt < 1:1506580/65‑1 (MQ=255) aaCGACCGCCAGAGGCGTAAACCCTCTG‑TTTTTTTTCATATGTAAACCTCGAGTGTCCGATTTCa > 1:3534181/1‑65 (MQ=255) aCGACCGCCAGAGGCGTAAACCCTCTG‑TTTTTTTTCATATGTAAACCTCGAGTGTCCGATTTaaa > 1:2960067/1‑65 (MQ=255) aCGACCGCCAGAGGCGTAAACCCTCTG‑TTTTTTTTCATATGTAAACCTCGAGTGTCCGATTTCaa > 1:3034545/1‑65 (MQ=255) aCGACCGCCAGAGGCGTAAACCCTCTG‑TTTTTTTTCATATGTAAACCTCGAGTGTCCGATTTCaa > 1:2999805/1‑65 (MQ=255) aCGACCGCCAGAGGCGTAAACCCTCTG‑TTTTTTTTCATATGTAAACCTCGAGTGTCCGATTTCaa > 1:1469541/1‑65 (MQ=255) cGCCAGAGGCGTAAACCCTCTG‑TTTTTTTTCATATGTAAACCTCGAGTGTCCGATTTCAAATtgg < 1:3910161/65‑1 (MQ=255) cGCCAGAGGCGTAAACCCTCTG‑TTTTTTTTCATATGTAAACCTCGAGTGTCCGATTTCAAATtgg > 1:507807/1‑65 (MQ=255) cGCCAGAGGCGTAAACCCTCTG‑TTTTTTTTCATATGTAAACCTCGAGTGTCCGATTTCAAATtgg < 1:2758178/65‑1 (MQ=255) gagGCGTAAACCGTCTG‑TTTTTTTTCATATGTAAACCTCGAGTGTCCGTTTTGAAATtggtgaat > 1:256540/1‑65 (MQ=255) gagGCGTAAACCCTCTG‑TTTTTTTTCATATGTAAACCTCGAGTGTCCGATTTCAAATtggtcaat > 1:2390620/1‑65 (MQ=255) gagGCGTAAACCCTCTG‑TTTTTTTTCATATGTAAACCTCGAGTGTCCGATTTCAAATtggtcaat > 1:933400/1‑65 (MQ=255) ggCGTAACCCCTCTG‑TTTTTTTTCATATGTAAGCCTCGAGTGTCCGATTTCAAATtggtcaatgg < 1:1638066/65‑1 (MQ=255) ggCGTAAACCCTCTG‑TTTTTTTTCATATGTAAACCTCGAGTGTCCGATTTCAAATtggtcaatgg > 1:2625667/1‑65 (MQ=255) ggCGTAAACCCTCTG‑TTTTTTTTCATATGTAAACCTCGAGTGTCCGATTTCAAATtggtcaatgg > 1:1814128/1‑65 (MQ=255) ggCGTAAACCCTCTG‑TTTTTTTTCATATGTAAACCTCGAGTGTCCGATTTCAAATtggtcaatgg > 1:2521635/1‑65 (MQ=255) ggCGTAAACCCTCTG‑TTTTTTTTCATATGTAAACCTCGAGTGTCCGATTTCAAATtggtcaatgg > 1:429310/1‑65 (MQ=255) cGTAAACCCTCTG‑TTTTTTTTCATATGTAAACCTCGAGTGTCCGATTTCAAATtggtcaatggtc > 1:15071/1‑65 (MQ=255) cGTAAACCCTCTG‑TTTTTTTTCATATGTAAACCTCGAGTGTCCGATTTCAAATtggtcaatggtc > 1:1614017/1‑65 (MQ=255) cGTAAACCCTCTG‑TTTTTTTTCATATGTAAACCTCGAGTGTCCGATTTCAAATtggtcaatggtc > 1:3353630/1‑65 (MQ=255) aCCCTCTG‑TTTTTTTTCATATGTAAACCTCGAGTGTCCGATTTCAAATTGGTCAATGGTCAAAAg > 1:3453903/1‑65 (MQ=255) aCCCTCTG‑TTTTTTTTCATATGTAAACCTCGAGTGTCCGATTTCAAATTGGTCAATGGTCAAAAg > 1:3435389/1‑65 (MQ=255) aCCCTCTG‑TTTTTTTTCATATGTAAACCTCGAGTGTCCGATTTCAAATTGGTCAATGGTCAAAAg > 1:814836/1‑65 (MQ=255) aCCCTCTG‑TTTTTTTTCATATGTAAACCTCGAGTGTCCGATTTCAAATTGGTCAATGGTCAAAAg > 1:946678/1‑65 (MQ=255) aCCCTCTG‑TTTTTTTTCATATGTAAACCTCGAGTGTCCGATTTCAAATTGGTCAATGGTCAAAAg > 1:3267379/1‑65 (MQ=255) aCCCTCTG‑TTTTTTTTCATATGTAAACCTCGAGTGTCCGATTTCAAATTGGTCAATGGTCAAAAg > 1:1296673/1‑65 (MQ=255) aCCCTCTG‑TTTTTTTTCATATGTAAACCTCGAGTGTCCGATTTCAAATTGGTCAATGGTCAAAAg > 1:1487975/1‑65 (MQ=255) aCCCTCTG‑TTTTTTTTCATATGTAAACCTCGAGTGTCCGATTTCAAATTGGTCAATGGTCAAAAg > 1:1797404/1‑65 (MQ=255) aCCCTCTG‑TTTTTTTTCATATGTAAACCTCGAGTGTCCGATTTCAAATTGGTCAATGGTCAAAAg > 1:1885318/1‑65 (MQ=255) aCCCTCTG‑TTTTTTTTCATATGTAAACCTCGAGTGTCCGATTTCAAATTGGTCAATGGTCAAAAg > 1:2095348/1‑65 (MQ=255) aCCCTCTG‑TTTTTTTTCATATGTAAACCTCGAGTGTCCGATTTCAAATTGGTCAATGGTCAAAAg > 1:248377/1‑65 (MQ=255) aCCCTCTG‑TTTTTTTTCATATGTAAACCTCGAGTGTCCGATTTCAAATTGGTCAATGGTCAAAAg > 1:2614913/1‑65 (MQ=255) aCCCTCTG‑TTTTTTTTCATATGTAAACCTCGAGTGTCCGATTTCAAATTGGTCAATGGTCAAAAg > 1:2743516/1‑65 (MQ=255) aCCCTCTG‑TTTTTTTTCATATGTAAACCTCGAGTGTCCGATTTCAAATTGGTCAATGGTCAAAAg > 1:2982790/1‑65 (MQ=255) aCCCTCTG‑TTTTTTTTCATATGTAAACCTCGAGTGTCCGATTTCAAATTGGTCAATGGTCAAAAg > 1:3328417/1‑65 (MQ=255) aCCCTCTG‑TTTTTTTTCATATGTAAACCTCGAGTGTCCGATTTCAAATTGGTCAATGGTCAAAAg > 1:3142019/1‑65 (MQ=255) aCCCTCTG‑TTTTTTTTCATATGTAAACCTCGAGTGTCCGATTTCAAATTGGCCAATGGTCAAAAg > 1:2615244/1‑65 (MQ=255) aCCCTCTG‑TTTTTTTTCATATGTAAAACTCGAGTGTCCGATTTCAAATTGGTCAATGGTCAAAAg > 1:3903316/1‑65 (MQ=255) aCCCTCTG‑TTTTTTTTCATATATAAACCTCGAGTGTCCGATTTCAAATTGGTCAATGGTCAAAAg > 1:1024592/1‑65 (MQ=255) cccTCTG‑TTTTTTTTCATATGTAAACCTCGAGTGTCCGATTTCAAATTGGTCAATGGTCAAAAGt > 1:1056171/1‑65 (MQ=255) cccTCTG‑TTTTTTTTCATATGTAAACCTCGAGTGTCCGATTTCAAATTGGTCAATGGTCAAAAGt > 1:1182416/1‑65 (MQ=255) cccTCTG‑TTTTTTTTCATATGTAAACCTCGAGTGTCCGATTTCAAATTGGTCAATGGTCAAAAGt > 1:610719/1‑65 (MQ=255) ccTCTG‑TTTTTTTTCATATGTAAACCTCGAGTGTCCGATTTCAAATTGGTCAATGGTCAAAAGtt > 1:2336320/1‑65 (MQ=255) ccTCTG‑TTTTTTTTCATATGTAAACCTCGAGTGTCCGATTTCAAATTGGTCAATGGTCAAAAGtt > 1:1972696/1‑65 (MQ=255) ctctG‑TTTTTTTTCATATGTAAACCTCGAGTGTCCGATTTCAAATTGGTCAATGGTCAAAAGTta < 1:873758/65‑1 (MQ=255) tctG‑TTTTTTTTCATATGTAAACCTCGAGTGTCCGATTTCAAATTGGTCAATGGTCAAAAGTtaa < 1:1618779/65‑1 (MQ=255) tctG‑TTTTTTTTCATATGTAAACCTCGAGTGTCCGATTTCAAATTGGTCAATGGTCAAAAGTtaa < 1:296369/65‑1 (MQ=255) ttttttTCATATGTAAACCTCGAGTGTCCGATTTCAAATTGGTCAATGGTCAAAAGTTAATAAAc > 1:1491943/1‑65 (MQ=255) ttttttTCATATGTAAACCTCGAGTGTCCGATTTCAAATTGGTCAATGGTCAAAAGTTAATAAAc < 1:2806495/65‑1 (MQ=255) ttttttTCATATGTAAACCTCGAGTGTCCGATTTCAAATTGGTCAATGGTCAAAAGTTAATAAAc < 1:1125461/65‑1 (MQ=255) ttttttTCATATGTAAACCTCGAGTGTCCGATTTCAAATTGGTCAATGGTCAAAAGTTAATAAAc < 1:2463162/65‑1 (MQ=255) ttttttTCATATGTAAACCTCGAGTGTCCGATTTCAAATTGGTCAATGGTCAAAAGTTAATAAAc < 1:3550724/65‑1 (MQ=255) | GTTAGGGCTAAGCTGCCTGAGAGCATCAGAACGACCGCCAGAGGCGTAAACCCTCTG‑TTTTTGTTCATATGTAAACCTCGAGTGTCCGATTTCAAATTGGTCAATGGTCAAAAGTTAATAAAC > NC_000913/485552‑485674 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |