Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,905,761	G→A	G25D (GGT→GAT)	mntP →	Mn(2(+)) exporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,905,761	0	G	A	100.0%	126.0 / NA	35	G25D (GGT→GAT)	mntP	Mn(2(+)) exporter
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (20/15);  total (20/15)

GCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGGTGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCC  >  NC_000913/1905700‑1905822                                                              |                                                              gCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGATGc                                                            >  1:913448/1‑65 (MQ=255)    aCTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGATGCCAc                                                         >  1:519405/1‑65 (MQ=255)    aCTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGATGCCAc                                                         >  1:1833359/1‑65 (MQ=255)    aCTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGATGCCAc                                                         <  1:875070/65‑1 (MQ=255)    aCTGTTCTTCTAGCTGTTGGTATGTCGATTGATGCATTTGCTGCATCAATCGGTAAAGATGCCAc                                                         <  1:3772555/65‑1 (MQ=255)         tcttctTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGATGCCACCCTcc                                                    <  1:1468578/65‑1 (MQ=255)         tcttctTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGATGCCACCCTcc                                                    >  1:1653017/1‑65 (MQ=255)         tcttctTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGATGCCACCCTcc                                                    <  1:2665741/65‑1 (MQ=255)           ttcttGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGATGCCACCCTCCAt                                                  >  1:1349476/1‑65 (MQ=255)           ttcttGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGATGCCACCCTCCAt                                                  >  1:3867169/1‑65 (MQ=255)           ttcttGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGATGCCACCCTCCAt                                                  >  1:2697404/1‑65 (MQ=255)            tcttGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGATGCCACCCTCCATa                                                 >  1:25877/1‑65 (MQ=255)                  gTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGATGCCACCCTCCATAAACCGa                                           <  1:61145/65‑1 (MQ=255)                            tCGATGGATGCATTTGCTGCATCAATCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGa                                 >  1:496702/1‑65 (MQ=255)                               atggatgCATTTGCTGCATCAATCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGc                              <  1:2488368/65‑1 (MQ=255)                               atggatgCATTTGCTGCATCAATCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGc                              <  1:505926/65‑1 (MQ=255)                               atggatgCATTTGCTGCATCAATCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGc                              <  1:3949781/65‑1 (MQ=255)                               atggatgCATTTGCTGCATCAATCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGc                              <  1:2144396/65‑1 (MQ=255)                               atggatgCATTTGCGGCATCAATCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGc                              <  1:1977905/65‑1 (MQ=255)                                 ggatgCATTTGCTGCATCAATCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCAt                            <  1:3005035/65‑1 (MQ=255)                                  gatgCATTTGCTGCATCAATCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCAtt                           >  1:1937473/1‑65 (MQ=255)                                  gatgCATTTGCTGCATCAATCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCAtt                           >  1:1045459/1‑65 (MQ=255)                                  gatgCATTTGCTGCATCAATCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCAtt                           >  1:872177/1‑65 (MQ=255)                                  gatgCATTTGCTGCATCAATCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCAtt                           >  1:1089624/1‑65 (MQ=255)                                  gatgCATTTGCTGCATCAATCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTCAAGCAtt                           >  1:3540006/1‑65 (MQ=255)                                     gCATTTGCTGCATCAATCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCg                        >  1:3156966/1‑65 (MQ=255)                                        tttGCTGCATCAATCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAAc                     >  1:3538971/1‑65 (MQ=255)                                         ttGCTGCATCAATCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAAcc                    >  1:1468184/1‑65 (MQ=255)                                         ttGCTGCATCAATCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAAcc                    >  1:1434063/1‑65 (MQ=255)                                           gctgcATCAATCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCgg                  <  1:2811670/65‑1 (MQ=255)                                           gctgcATCAATCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCgg                  <  1:882092/65‑1 (MQ=255)                                           gctgcATCAATCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCgg                  <  1:576734/65‑1 (MQ=255)                                            ctgcATCAATCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGc                 <  1:3718150/65‑1 (MQ=255)                                             ggcATCAATCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGcc                <  1:2517459/64‑1 (MQ=255)                                                       ggTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGCCTTATTTTTgg      >  1:1060890/1‑65 (MQ=255)                                                           aaGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGcc  >  1:523544/1‑65 (MQ=255)                                                           aaGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGcc  >  1:2061116/1‑65 (MQ=255)                                                              |                                                              GCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGGTGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCC  >  NC_000913/1905700‑1905822

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑