| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,232,468 | 0 | A | G | 54.5% | ‑4.2 / 20.8 | 22 | intergenic (+14/+32) | umuC/dsbB | DNA polymerase V catalytic protein/protein thiol:quinone oxidoreductase DsbB |
| Reads supporting (aligned to +/- strand): ref base A (5/5); new base G (12/0); total (17/5) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 9.57e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.26e-02 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGC > NC_000913/1232405‑1232529 | tttCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGaa > 1:1936104/1‑65 (MQ=255) ttCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGgaa > 1:1713666/1‑65 (MQ=255) accacGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGaaaaaa < 1:1441746/65‑1 (MQ=255) ccacGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGaaaaaaa > 1:1215622/1‑64 (MQ=255) ttATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTc < 1:546499/65‑1 (MQ=255) aCAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGGAGAAAGCccgcccgc > 1:2542295/1‑65 (MQ=255) aCAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGGAAAAAGCGCTCCCGc > 1:2315426/1‑65 (MQ=255) aCAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGGAAAAAGCGCGCCCGc > 1:981221/1‑65 (MQ=255) aCAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGGAAAAAGCGCCCCCGc > 1:2507495/1‑65 (MQ=255) aCAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGGAAAAAGCACTccccc > 1:1570140/1‑63 (MQ=255) aCAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGGAAAAAACGCcccccc > 1:646889/1‑63 (MQ=255) aCAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGGAAAAAACGCTccccc > 1:2087063/1‑63 (MQ=255) aCAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGGAAAAAACGCTCCCGc > 1:1525481/1‑65 (MQ=255) aCAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGGAAAAAACGCTCCCGc > 1:1774880/1‑65 (MQ=255) aCAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGGAAAAAACGCTCCCGc > 1:1099643/1‑65 (MQ=255) aCAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGGAAAAAACGCGccccc > 1:2409381/1‑63 (MQ=255) aCAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGGAAAAAACGCCACCGc > 1:2769184/1‑65 (MQ=255) aCAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGc > 1:2326394/1‑65 (MQ=255) aCAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGAGCCCCCGc > 1:2336292/1‑65 (MQ=255) aCAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAACGCcccccc > 1:683129/1‑63 (MQ=255) aCAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAACGCTCCCGc > 1:1663057/1‑65 (MQ=255) aCAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAACGCTCCCGc > 1:152958/1‑65 (MQ=255) aCAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAACGCTCCCGc > 1:1287289/1‑65 (MQ=255) gcgAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGTTGCCGCTGGaa > 1:1220114/1‑64 (MQ=255) gTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGGAAAAAACGCCCCCGCAGGAGGGCCg > 1:1192516/1‑65 (MQ=255) tGATTTACTGCGGGTCAAACAAATATAGCGGCAGAAAAAAAGCGCTCCCGCAGGAGCGCCGAATg < 1:647497/65‑1 (MQ=255) gATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATgg < 1:2524130/65‑1 (MQ=255) tttACTGAGGGTCAAATAAATATAGCGGCAGGGAAAAAACGCTCCCGCAGGAGCGCCGAATGGac > 1:1472723/1‑64 (MQ=255) tttACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGAt < 1:1452448/65‑1 (MQ=255) aCTGAGGGTCAAATAAATATAGCGGCAGGGAAAAAACGCTCCCGCAGGAGCGCCGAATGGATTAg > 1:579331/1‑65 (MQ=255) tGAGGGTCAAATAAATATAGCGGCAGGGAAAAAACGCCCCCGCACGAGCGCCCAATGGATTAGCg > 1:1703879/1‑65 (MQ=255) gTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCCAGAGCGCCGAAAGGCTTAACGAccca > 1:94762/1‑63 (MQ=255) gTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGa < 1:551963/65‑1 (MQ=255) gTCAAATAAATATAGCGGCAGGAAAAAAGCGCCCCCGCAGGAGCGCCGAATGGATTAGCGACCGa > 1:1228069/1‑65 (MQ=255) ataaataTAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGa < 1:1232401/65‑1 (MQ=255) ataaataTAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGa < 1:96787/65‑1 (MQ=255) ataaataTAGCGGCAGGAAAAAAACGCTCCCGCACGAGCGCCGACAGGATTAGCGACCGAACAGa > 1:2073364/1‑65 (MQ=255) ataaataGAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGa < 1:328349/65‑1 (MQ=255) taaataTAGCGGCAGGGAAAAAGCGCTCCCGCCGGAGCGCCGAATGGATTAGCGACCCAACacaa > 1:480979/1‑62 (MQ=255) taaataTAGCGGCAGGGAAAAAACGCTGCCGCCGGAGCGCCGAATGGATTAGCGACCGAACAGAt > 1:424191/1‑65 (MQ=255) taaataTAGCGGCAGGAAAAAAGCGCTCCCGCCGGGGCGCCGAATGGATTAACGAACGAACAGAt > 1:1919928/1‑65 (MQ=255) aGGAAAATAGCGCTCCCGCAGGAGCGGCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGc < 1:1331220/65‑1 (MQ=255) | TTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGC > NC_000913/1232405‑1232529 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |