| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 49,765 | C→T | intergenic (+134/‑58) | kefC → / → folA | K(+) : H(+) antiporter KefC/dihydrofolate reductase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 49,765 | 0 | C | T | 98.4% | 222.6 / ‑4.3 | 61 | intergenic (+134/‑58) | kefC/folA | K(+) : H(+) antiporter KefC/dihydrofolate reductase |
| Reads supporting (aligned to +/- strand): ref base C (0/1); new base T (24/36); total (24/37) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.49e-01 | |||||||||||
GGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGA > NC_000913/49703‑49826 | ggAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTtga > 1:628144/1‑65 (MQ=255)ggAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTtga > 1:2352507/1‑65 (MQ=255)ggAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTtga > 1:1484603/1‑65 (MQ=255)ggAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTtga > 1:287621/1‑65 (MQ=255) agagagCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTtgacg > 1:2938853/1‑65 (MQ=255) agagagCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTtgacg > 1:1233110/1‑65 (MQ=255) agagCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCACCATCATCCTCGCATCAGTTgacgac > 1:225383/1‑65 (MQ=255) agagCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTgacgac > 1:548620/1‑65 (MQ=255) ggCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGACGACgg < 1:1504707/64‑1 (MQ=255) agCGTTGCCTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACgg < 1:1388401/65‑1 (MQ=255) agCGTGGACTCGCCGGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGACGACgg > 1:2296072/1‑65 (MQ=255) agCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGACGACgg < 1:1981195/65‑1 (MQ=255) agCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGACGACgg < 1:593699/65‑1 (MQ=255) agCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGACGACgg > 1:1045551/1‑65 (MQ=255) agCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGACGACgg < 1:1947758/65‑1 (MQ=255) agCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGACGACgg > 1:1900701/1‑65 (MQ=255) agCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGACGACgg < 1:2039355/65‑1 (MQ=255) agCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGACGACgg < 1:1837239/65‑1 (MQ=255) agCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGACGACgg > 1:1789909/1‑65 (MQ=255) agCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGACGACgg > 1:1722545/1‑65 (MQ=255) agCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGACGACgg > 1:2439580/1‑65 (MQ=255) agCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGACGACgg < 1:2789043/65‑1 (MQ=255) agCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGACGACgg < 1:680646/65‑1 (MQ=255) agCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGACGACgg < 1:1125585/65‑1 (MQ=255) agCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGACGACgg > 1:856019/1‑65 (MQ=255) agCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGACGACgg > 1:932096/1‑65 (MQ=255) gCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGACGACGGt < 1:1652461/65‑1 (MQ=255) gCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGACGACGGt < 1:1395636/65‑1 (MQ=255) gCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGACGACGGt < 1:311507/65‑1 (MQ=255) gACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGACGACGGTTTACg > 1:1863989/1‑65 (MQ=255) aCTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGACGACGGTTTACGc > 1:1250577/1‑65 (MQ=255) aCTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCATCGCACCAGTTGACGACGGTTTACGc > 1:2944825/1‑65 (MQ=255) cTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGACGACGGTTTACGCt > 1:612155/1‑65 (MQ=255) cTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGACGACGGTTTACGCt < 1:483118/65‑1 (MQ=255) tCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGACGACGGTTTACGCtt < 1:2934301/65‑1 (MQ=255) tCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGACGACGGTTTACGCtt < 1:1651066/65‑1 (MQ=255) tCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGACGACGGTTTACGCtt < 1:756486/65‑1 (MQ=255) ccAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCGGTTGACGACGGTTTACGCTTTAc < 1:184464/65‑1 (MQ=255) ccAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGACGACGGTTTACGCTTTAc < 1:2982949/65‑1 (MQ=255) ccAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGACGACGGTTTACGCTTTAc < 1:2945556/65‑1 (MQ=255) ccAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGACGACGGTTTACGCTTTAc < 1:1493720/65‑1 (MQ=255) ccAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGACGACGGTTTACGCTTTAc < 1:1352712/65‑1 (MQ=255) ccAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGACGACGGTTTACGCTTTAc > 1:2882522/1‑65 (MQ=255) agcagAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGACGACGGTTTACGCTTTACGt < 1:455998/65‑1 (MQ=255) agcagAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGACGACGGTTTACGCTTTACGt < 1:660498/65‑1 (MQ=255) agcagAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGACGACGGTTTACGCTTTACGt < 1:982570/65‑1 (MQ=255) agcagAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGACGACGGTTTACGCTTTACGt < 1:1741813/65‑1 (MQ=255) gcagAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGACGACGGTTTACGCTTTACGta > 1:2823971/1‑65 (MQ=255) agAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGACGACGGTTTACGCTTTACGtata > 1:1099133/1‑65 (MQ=255) agAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGACGACGGTTTACGCTTTACGtata > 1:663729/1‑65 (MQ=255) agAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGACGACGGTTTACGCTTTACGtata > 1:1965607/1‑65 (MQ=255) agAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGACGACGGTTTACGCTTTACGtata > 1:599716/1‑65 (MQ=255) atatAAAATTTTCCTCAACATCATCCTCGCACCAGTTGACGACGGTTTACGCTTTACGTATAGTg < 1:2610730/65‑1 (MQ=255) aaaTTTTCCTCAACATCATCCTCGCACCAGTTGACGACGGTTTACGCTTTACGTATAGTGGCGAc > 1:195201/1‑65 (MQ=255) aTTTTCCTCAACATCATCCTCGCACCAGTTGACGACGGTTTACGCTTTACGTATAGTGGCGACaa > 1:2498722/1‑65 (MQ=255) aTTTTCCTCAACATCATCCTCGCACCAGTTGACGACGGTTTACGCTTTACGTATAGTGGCGACaa > 1:1046929/1‑65 (MQ=255) aTTTTCCTCAACATCATCCTCGCACCAGTTGACGACGGTTTACGCTTTACGTATAGTGGCGACaa > 1:2057059/1‑65 (MQ=255) ttCCTCAACATCATCCTCGCACCAGTTGACGACGGTTTACGCTTTACGTATAGTGGCGACAAttt < 1:435581/65‑1 (MQ=255) atcatcCTCGCACCAGTTGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCg < 1:1682727/65‑1 (MQ=255) ccTCGCACCAGTTGACGACGGTTTACGCTTTACGTATAGTGGCGACCATTTTTTTTATTGGGaaa > 1:731451/1‑65 (MQ=255) ccTCGCACCAGTTGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTTTCGGGaaa > 1:1198135/1‑65 (MQ=255) ccTCGCACCAGTTGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGaaa > 1:1432027/1‑65 (MQ=255) cTCGCACCAGTTGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAAt < 1:2383029/65‑1 (MQ=255) tCGCACCAGTTGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAAtc < 1:208965/65‑1 (MQ=255) tCGCACCAGTTGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAAtc < 1:1222154/65‑1 (MQ=255) tCGCACCAGTTGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAAtc < 1:764244/65‑1 (MQ=255) gCACCAGTTGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAAtctc < 1:837310/65‑1 (MQ=255) ccAGTTGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAAt > 1:2339604/1‑65 (MQ=255) aGTTGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGa < 1:2907834/65‑1 (MQ=255) aGTTGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGa < 1:835776/65‑1 (MQ=255) aGTTGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGa < 1:1021669/65‑1 (MQ=255) | GGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGA > NC_000913/49703‑49826 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |