Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,390,754	G→T	A252A (GCC→GCA)	rsgA ←	ribosome small subunit‑dependent GTPase A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,390,754	0	G	T	100.0%	63.7 / NA	19	A252A (GCC→GCA)	rsgA	ribosome small subunit‑dependent GTPase A
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (17/2);  total (17/2)

CTCACGCACTCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGTGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGG  >  NC_000913/4390694‑4390808                                                             |                                                       cTCACGCACTCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAgctgctg                                                    >  1:345725/1‑63 (MQ=255)      gCACTCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGTCgtg                                               >  1:2869925/1‑65 (MQ=255)        aCTCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGTCgtggt                                             >  1:1193018/1‑65 (MQ=255)        aCTCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGTCggggt                                             >  1:1479717/1‑65 (MQ=255)        aCTCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGTCgcggt                                             >  1:1952926/1‑65 (MQ=255)                  aGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGGTGTGCTGActc                                   >  1:410348/1‑63 (MQ=255)                  aGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGGTGTGCTGACCg                                   >  1:870486/1‑65 (MQ=255)                  aGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGGTGTGCTGACCg                                   >  1:364278/1‑65 (MQ=255)                  aGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGGTGTGCTGACCg                                   >  1:2579815/1‑65 (MQ=255)                  aGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGTCGCGGTGGGCTGACCg                                   >  1:2961558/1‑65 (MQ=255)                        tcacatcacCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGGTGTGCTGACCGAGcccc                             >  1:1787881/1‑65 (MQ=255)                          acatcacCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGGTGTGCTGACCGAGCCCCGa                           >  1:2688428/1‑65 (MQ=255)                           catcccCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGGTGTGCTGACCGAGCCCCGAg                          <  1:2734838/65‑1 (MQ=255)                            atcgcCGCCGTGCGGGAAGTGATCCAGCCGATCTGCGGTCGTGGTGTGCTGACCGAGCCCCGAGt                         <  1:1765187/65‑1 (MQ=255)                             tcacCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGGTGTGCTGACCGAGCCCCGAGtt                        >  1:1453100/1‑65 (MQ=255)                             tcacCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGGAGTGCTGACCGACCCCCGAGtt                        >  1:353747/1‑65 (MQ=255)                                  gccgTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGGTGTGCTGACCGAGCCCCGAGTTGTCag                   >  1:366443/1‑65 (MQ=255)                                  gccgTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGGTGTGCTGACCGAGCCCCGAGTTGTCag                   >  1:796566/1‑65 (MQ=255)                                  gccgTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGGTGTGCTGACCGAGCCCCGAGTTGTCag                   >  1:81613/1‑65 (MQ=255)                                  gccgTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGGTGTGCTGACCGAGCCCCGAGCTGTCag                   >  1:2038737/1‑65 (MQ=255)                                     gTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGGTGTGCTGACCGAGCCCCGAGTTGTCAgaga                <  1:1630101/65‑1 (MQ=255)                                       gCGGGAAGTGATACAGCCGAGCTGCGGTCGTGGTGTGCTGACCGAGCCCCGAGTTGCCAGAGata              >  1:130486/1‑65 (MQ=255)                                                 aTACAGCCGAGCTGCGGTCGTGGTGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCa    >  1:1822423/1‑65 (MQ=255)                                                   aCAGCCGAGCTGCGGTCGTGGTGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAgg  >  1:2727649/1‑65 (MQ=255)                                                   aCAGCCGAGCTGCGGTCGTGGTGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAgg  <  1:1498171/65‑1 (MQ=255)                                                   aCAGCCGAGCTGCGGTCGTGGTGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAgg  >  1:874184/1‑65 (MQ=255)                                                   aCAGCCGAGCTGCGGTCGTGGTGTGCTGACCGAGCCCCGAGCTGCCAGAGATATCGTTGGTCatg  >  1:1151265/1‑63 (MQ=255)                                                             |                                                       CTCACGCACTCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGTGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGG  >  NC_000913/4390694‑4390808

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑