Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	49,884	C→T	P21L (CCG→CTG)	folA →	dihydrofolate reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	49,884	0	C	T	100.0%	51.7 / NA	16	P21L (CCG→CTG)	folA	dihydrofolate reductase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (9/7);  total (9/7)

AATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAAC  >  NC_000913/49822‑49937                                                               |                                                      aaTGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCTGt                                                     <  1:198840/65‑1 (MQ=255)  aTGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCtgtg                                                    <  1:2022194/65‑1 (MQ=255)    gATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCTGTGGa                                                  <  1:856970/65‑1 (MQ=255)    gATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCTGTGGa                                                  >  1:1550616/1‑65 (MQ=255)    gATCAGTCTGATTGCGGCGTGAGCGGTAGATCGCGTTATCGGGATGTAAAACGCCATGCTGTGGa                                                  <  1:83341/65‑1 (MQ=255)      tCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCTGTGGAAc                                                <  1:705481/65‑1 (MQ=255)       cAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGGAAACGCCATGCCGTGGGAcc                                               >  1:240005/1‑65 (MQ=255)         gTCTGATGGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCTGTGGAAcctg                                             <  1:2848713/65‑1 (MQ=255)         gTCGGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCTGTGGAAcctg                                             <  1:242932/65‑1 (MQ=255)           cTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCCTGCTGGGGAAcgtgcc                                           >  1:786605/1‑65 (MQ=255)                tGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCTGTGGAACCTGCCTGCCg                                      >  1:72285/1‑65 (MQ=255)                 gcggcgTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATTCTGCGGAACCTCCCTGCCGa                                     >  1:731953/1‑65 (MQ=255)                      gTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCTGTGGACCCTGCCTGCCGATcgcg                                <  1:478240/65‑1 (MQ=255)                           cGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCTGTGGAACCTGCCTGCCGATCGCGCCTgg                           <  1:2958332/65‑1 (MQ=255)                             gTAGATCGCGTTATCGGCATGGAAAACGCCATGCTGTGGAACCTGCCTGCCGATCGCGCCTGGtt                         >  1:1873399/1‑65 (MQ=255)                                gATCGCGTTATCGGCATGGAAAACGCCATGGTGTGGAACCTACCTGCCGATCGCGCCTGGTTTaa                      <  1:49869/65‑1 (MQ=255)                                 aTCGCGTTATCGGCATGGAAAACGCCATGCTGTGGGACCTGCCTGCCGATCGCGCCCGGTTTaaa                     >  1:1843244/1‑65 (MQ=255)                                 aTCGCGTTATCGGCATGGAAAACGCCATGCTGTGGAACCTGCCTGCCGATCGCGCCTGGTTTaaa                     >  1:2054987/1‑65 (MQ=255)                                 aTCGCGTTATCGGCATGGAAAACGCCATGCTGTGGAACCTGCCTGCCGATCGCGCCTGGTTTaaa                     >  1:324641/1‑65 (MQ=255)                                 aTCGCGTTATCGGCATGGAAAACGCCATGCTGTGGAACCTGCCTGCCGATCGCGCCTGGTTTaaa                     >  1:353757/1‑65 (MQ=255)                                 aTCGCGTTATCGGCATGGAAAACGCCATGCTGTGGAACCTGCCTGCCGATCGCGCCTGGTTTaaa                     >  1:1781794/1‑65 (MQ=255)                                  tCGCGTTATCGGCATGGAAAACGCCATGCTGTGGAACCTGCCTGCCGATCGCGCCTGGTTTAAAc                    <  1:2387671/65‑1 (MQ=255)                                        tATCGGCATGGAAAACGCCCTGCTGTGGAACCTGCCTGCCGATCGCGCCTGGTTTAAACGCAaca              >  1:689458/1‑65 (MQ=255)                                         gtCGTCATGGAAAGCTCCTTGGTGTTGAACCGGCCTGCCGATCGCGCCTGGTTTAAACGCAacac             <  1:1471133/64‑1 (MQ=255)                                         aTCGGCATGGAAAACCCCATGCTGTGGACCCTGCCTGCCGATCGCGCCTGGTTTAAACGCAacac             <  1:939878/65‑1 (MQ=255)                                            ggCATGGAAAACGCCATGCTGTGGAACCTGCCTGCCGATCGCGCCTGGTTTAAACGCAACACCtt          >  1:971769/1‑65 (MQ=255)                                              cATGGAAACCGCCATGCTGTGGAACCTGCCTGCCGATCGCGCCTGGTTTAAACGCAACACCTtaa        <  1:2204467/65‑1 (MQ=255)                                                    aaaCGCCAGGCCGTGGAACCTGCCTGCCGACCGCGCCAGGTTTAAACGCAACACCTTAAATAAAc  <  1:1461728/65‑1 (MQ=255)                                                               |                                                      AATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAAC  >  NC_000913/49822‑49937

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑