Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	49,905	T→G	L28R (CTC→CGC)	folA →	dihydrofolate reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	49,905	0	T	G	100.0%	64.9 / NA	20	L28R (CTC→CGC)	folA	dihydrofolate reductase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (13/7);  total (13/7)

GTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTG  >  NC_000913/49843‑49962                                                               |                                                          gTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCTGTGGACCCTGCCTGCCGATcgcg                                                         <  1:478240/65‑1 (MQ=255)      cGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCTGTGGAACCTGCCTGCCGATCGCGCCTgg                                                    <  1:2958332/65‑1 (MQ=255)        gTAGATCGCGTTATCGGCATGGAAAACGCCATGCTGTGGAACCTGCCTGCCGATCGCGCCTGGtt                                                  >  1:1873399/1‑65 (MQ=255)           gATCGCGTTATCGGCATGGAAAACGCCATGGTGTGGAACCTACCTGCCGATCGCGCCTGGTTTaa                                               <  1:49869/65‑1 (MQ=255)            aTCGCGTTATCGGCATGGAAAACGCCATGCTGTGGGACCTGCCTGCCGATCGCGCCCGGTTTaaa                                              >  1:1843244/1‑65 (MQ=255)            aTCGCGTTATCGGCATGGAAAACGCCATGCTGTGGAACCTGCCTGCCGATCGCGCCTGGTTTaaa                                              >  1:1781794/1‑65 (MQ=255)            aTCGCGTTATCGGCATGGAAAACGCCATGCTGTGGAACCTGCCTGCCGATCGCGCCTGGTTTaaa                                              >  1:2054987/1‑65 (MQ=255)            aTCGCGTTATCGGCATGGAAAACGCCATGCTGTGGAACCTGCCTGCCGATCGCGCCTGGTTTaaa                                              >  1:353757/1‑65 (MQ=255)            aTCGCGTTATCGGCATGGAAAACGCCATGCTGTGGAACCTGCCTGCCGATCGCGCCTGGTTTaaa                                              >  1:324641/1‑65 (MQ=255)             tCGCGTTATCGGCATGGAAAACGCCATGCTGTGGAACCTGCCTGCCGATCGCGCCTGGTTTAAAc                                             <  1:2387671/65‑1 (MQ=255)                   tATCGGCATGGAAAACGCCCTGCTGTGGAACCTGCCTGCCGATCGCGCCTGGTTTAAACGCAaca                                       >  1:689458/1‑65 (MQ=255)                    gtCGTCATGGAAAGCTCCTTGGTGTTGAACCGGCCTGCCGATCGCGCCTGGTTTAAACGCAacac                                      <  1:1471133/64‑1 (MQ=255)                    aTCGGCATGGAAAACCCCATGCTGTGGACCCTGCCTGCCGATCGCGCCTGGTTTAAACGCAacac                                      <  1:939878/65‑1 (MQ=255)                       ggCATGGAAAACGCCATGCTGTGGAACCTGCCTGCCGATCGCGCCTGGTTTAAACGCAACACCtt                                   >  1:971769/1‑65 (MQ=255)                         cATGGAAACCGCCATGCTGTGGAACCTGCCTGCCGATCGCGCCTGGTTTAAACGCAACACCTtaa                                 <  1:2204467/65‑1 (MQ=255)                               aaaCGCCAGGCCGTGGAACCTGCCTGCCGACCGCGCCAGGTTTAAACGCAACACCTTAAATAAAc                           <  1:1461728/65‑1 (MQ=255)                                        gctgTGGAACCTGCCTGCCGATCGCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTa                  >  1:2755399/4‑65 (MQ=255)                                        gctgTGGAACCTGCCTGCCGATCGCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTa                  >  1:2176000/4‑65 (MQ=255)                                               aaCCTGCCTGCCGATCGCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGgccg           >  1:1554292/1‑65 (MQ=255)                                               aaCCTGCCTGCCGATCGCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGgccg           >  1:943110/1‑65 (MQ=255)                                                aCCTGCCTGCCGATCGCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGgccgc          >  1:2781491/1‑65 (MQ=255)                                                aCCTGCCTGCCGATCGCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGgccgc          >  1:2332426/1‑65 (MQ=255)                                                 cctgcctgCCGATCGCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGgccgcc         >  1:730810/1‑65 (MQ=255)                                                  ctgcctgcCGATCGCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCa        >  1:2602761/1‑65 (MQ=255)                                                  ctgcctgcCGATCGCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCa        >  1:2006626/1‑65 (MQ=255)                                                  ctgcctgcCGATCGCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCa        >  1:617289/1‑65 (MQ=255)                                                     cctgccGATCGCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATAc     >  1:1154722/1‑65 (MQ=255)                                                        gccGATCGCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTg  >  1:1908852/1‑65 (MQ=255)                                                               |                                                          GTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTG  >  NC_000913/49843‑49962

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑