Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,905,761	G→A	G25D (GGT→GAT)	mntP →	Mn(2(+)) exporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,905,761	0	G	A	100.0%	124.4 / NA	35	G25D (GGT→GAT)	mntP	Mn(2(+)) exporter
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (21/14);  total (21/14)

TGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGGTGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGCCTTAT  >  NC_000913/1905699‑1905812                                                               |                                                    tGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGATg                                                   >  1:466123/1‑65 (MQ=255)  gCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGATGc                                                  <  1:309872/65‑1 (MQ=255)    tACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGATGCCa                                                >  1:2392196/1‑65 (MQ=255)     aCTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGATGCCAc                                               >  1:373705/1‑65 (MQ=255)     aCTGTTCTTCTTGCGTGTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGATGCCAc                                               <  1:778198/65‑1 (MQ=255)     aCTGTGCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGATGCCAc                                               <  1:86463/65‑1 (MQ=255)       tGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGATGCCAccc                                             >  1:1712699/1‑65 (MQ=255)          tcttctTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGATGCCACCCTcc                                          >  1:2653766/1‑65 (MQ=255)          tcttctTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGATGCCACCCTcc                                          <  1:580593/65‑1 (MQ=255)          tcttctTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGATGCCACCCTcc                                          <  1:1928797/65‑1 (MQ=255)          tcttctTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGATGCCACCCTcc                                          <  1:1391071/65‑1 (MQ=255)            ttcttGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGATGCCACCCTCCAt                                        >  1:1623255/1‑65 (MQ=255)               ttGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGATGCCACCCTCCATaaa                                     <  1:1804450/65‑1 (MQ=255)                    tttGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGATGCCACCCCCCATAAACCGaa                                >  1:2008990/1‑65 (MQ=255)                       ggTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGATGCCACCCTCCATAAACCGAAAtt                             >  1:1615632/1‑65 (MQ=255)                        gTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGATGCCACCCTCCATAAACCGAAAttt                            >  1:549905/1‑65 (MQ=255)                         tATGTCGATGGATGCATTTGCTGCATCAAGCGGTAAAGATGCCACCCTCCATAAACCGAAAtttt                           <  1:1819142/65‑1 (MQ=255)                                atggatgCGTTTGCTGCATCAATGGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGc                    <  1:2847779/65‑1 (MQ=255)                                atggatgCATTTCCTGCATCAATCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGc                    <  1:907683/65‑1 (MQ=255)                                 tggatgCATTTGCTGCATCAATCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCa                   >  1:256967/1‑65 (MQ=255)                                 tggatgCATTTGCTGCATCAATCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCa                   >  1:2163804/1‑65 (MQ=255)                                   gatgCATTTGCTGCATCAATCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCAtt                 >  1:2656890/1‑65 (MQ=255)                                   gatgCATTTGCTGCATCAATCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCAtt                 >  1:1104027/1‑65 (MQ=255)                                   gatgCATTTGCTGCATCAATCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCAtt                 >  1:842912/1‑65 (MQ=255)                                       cATTTGCTGCATCAATCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGa             <  1:2022459/65‑1 (MQ=255)                                          ttGCTGCATCAATCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAAcc          >  1:2898517/1‑65 (MQ=255)                                          ttGCTGCATCAATCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAAcc          >  1:812432/1‑65 (MQ=255)                                           tgctgcATCAATCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCg         >  1:1860161/1‑65 (MQ=255)                                            gctgcATCAATCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCgg        >  1:2048509/1‑65 (MQ=255)                                            gctgcATCAATCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCgg        >  1:559339/1‑65 (MQ=255)                                            gctgcATCAATCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCgg        >  1:2728551/1‑65 (MQ=255)                                             ctgcATCAATCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGc       <  1:2924219/65‑1 (MQ=255)                                             ctgcATCAATCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGc       <  1:2551203/65‑1 (MQ=255)                                             ctgcATCAATCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGc       <  1:2552026/65‑1 (MQ=255)                                             ctgcATCAATCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGc       >  1:90397/1‑65 (MQ=255)                                              tgcATCAATCGGTAAAGATGCCACCCTCCACAAACCGAAATTTTCTGAAGCATTGCCAACCGGcc      >  1:2069946/1‑65 (MQ=255)                                                  tCAATCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGCCTTAt  <  1:1939658/65‑1 (MQ=255)                                                               |                                                    TGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGGTGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGCCTTAT  >  NC_000913/1905699‑1905812

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑