| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 310,934 | 0 | A | C | 52.0% | 15.2 / 39.8 | 25 | F135V (TTC→GTC) | ecpR | DNA‑binding transcriptional dual regulator MatA |
| Reads supporting (aligned to +/- strand): ref base A (11/1); new base C (0/13); total (11/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.69e-06 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.92e-04 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
ATGCGGATAATTTCCATTTCCCGGTCCGTGATTTTATCTTTCTTAATATCTTTTCTCAGGAACCTGCCATTAATCACATAGACCAGTTCTTTTCTAATATCACGACTCAGTCCAGCGTATACC > NC_000913/310873‑310995 | aTGCGGATAATTTCCATTTCCCGGTCCGTGATTTTATCTTTCTTAATATCTTTTCTCAGGAACCt > 1:1553510/1‑65 (MQ=255) tGCGGATAATTTCCATTTCCCGGTCCGTGATTTTATCTTTCTTAATATCTTTTCTCAGGAACCTg > 1:3852178/1‑65 (MQ=255) ggATAATTTCCATTTCCCGGTCCGTGATTTTATCTTTCTTAATATCTTTTCTCAGGAACCTGCCa > 1:1543874/1‑65 (MQ=255) aTAATTTCCATTTCCCGGTCCGTGATTTTATCTTTCTTAATATCTTTTCTCAGGAACCTGCCAtt > 1:135434/1‑65 (MQ=255) aTAATTTCCATTTCCCGGTCCGTGATTTTATCTTTCTTAATATCTTTTCTCAGGAACCTGCCAtt > 1:912945/1‑65 (MQ=255) aTAATTTCCATTTCCCGGTCCGTGATTTTATCTTTCTTAATATCTTTTCTCAGGAACCTGCCAtt > 1:3720501/1‑65 (MQ=255) tccatttccCGGTCCGTGATTTTATCTTTCTTAATATCTTTTCTCAGGAACCTGCCATTAATcac > 1:2849482/1‑65 (MQ=255) tccattcccCGGTCCGTGTTTTTTTCTTTCTTAATATCTTTTCTCAGGAACCTGCCATTAATcac < 1:3622553/65‑1 (MQ=255) tccattcccCGGTCCGTGATTTTTTCTTTCTTAATATTTTTTCCCAGGACCCTGCCATTAATcac < 1:3649642/65‑1 (MQ=255) ttccCGGTCCGTGTTTTTTTCTTTCTTAATATCTTTTCTCAGGAACCTGCCATTAATCACATAGa < 1:1905177/65‑1 (MQ=255) ttccCGGTCCGTGTTTTTATCTTTCTTAATATCTTTTCTCAGGACCCTGCCATTAATCACATAGa < 1:2002497/65‑1 (MQ=255) ttccCGGTCCGTGTTTTTATCTTTCTTAATATCTTTTCTCAGGAACCTGCCATTAATCACATAGa < 1:1016968/65‑1 (MQ=255) ttccCGGTCCGTGATTTTATTTTTCTTAATATCTTTTCCCAGGACCCTGCCATTAATCACATAGa < 1:554402/65‑1 (MQ=255) ttccCGGTCCGTGATTTTATCTTTCTTAATATCTTTTCTCAGGACCCTGCCATTAATCACATAGa < 1:3879905/65‑1 (MQ=255) ttccCGGTCCGTGATTTTATCTTTCTTAATATCTTTTCTCAGGACCCTGCCATTAATCACATAGa < 1:195127/65‑1 (MQ=255) ttccCGGTCCGTGATTTTATCTTTCTTAATATCTTTTCTCAGGACCCTGCCATTAATCACATAGa < 1:75573/65‑1 (MQ=255) tccccGGCCCGTGATTTTATCTTTCTTAATATCTTTACCAAGAACCCTGCCATTAATCACATAGa < 1:2452459/63‑1 (MQ=255) ccGGTCCGTGATTTTATCTTTCTTAATATCTTTTCTCAGGAACCTGCCATTAATCACATAGACCa > 1:972720/1‑65 (MQ=255) cGGTCCGTGATTTTATCTTTCTTAATATCTTTTCTCAGGAACCTGCCATTAATCACATAGACCAg > 1:2443919/1‑65 (MQ=255) ggTCCGTGTTTTTATCTTTCTTAATATCTTTTCTCAGGACCCTGCCATTAATCACATAGACCAGt < 1:3323234/65‑1 (MQ=255) ggTCCGTGATTTTATCTTTCTTAATATCTTTTCTCAGGACCCTGCCATTAATCACATAGACCAGt < 1:1230877/65‑1 (MQ=255) ggTCCGTGATTTTATCTTTCTTAATATCTTTTCTCAGGACCCTGCCATTAATCACATAGACCAGt < 1:2353591/65‑1 (MQ=255) cgTCCTTGTTTTTATTTTTCTTAATATTTTTCCGAAGAACCCTGCCATTAATCACATAGACCAGt < 1:965385/64‑1 (MQ=255) ccGTGTTTTTATCTTTCTTAATATCTTTTCTCAGGACCCTGCCATTAATCACATAGACCAGTTCt < 1:908702/65‑1 (MQ=255) aTTTTATCTTTCTTAATATCTTTTCTCAGGAACCTGCCATTAATCACATAGACCAGTTCTTTTCt < 1:6560/65‑1 (MQ=255) aTTTTATCTTTCTAAATATCTTTTCCCAGGACCCTGCCATTAATCACATAGACCAGTTCTTTTCt < 1:3151505/65‑1 (MQ=255) tATCTTTCTTAATTTCTTTTCTCAGGACCCTGCCATTAATCACATAGACCAGTTCTTTTCTAata < 1:843768/65‑1 (MQ=255) tttttcttAATATCTTTTCTCAGGACCCTGCCATTAATCACATAGACCAGTTCTTTTCTAATATc < 1:460018/63‑1 (MQ=255) tctttcttAATTTCTTTTCTCAGGACCCTGCCATTAATCACATAGACCAGTTCTTTTCTAATATc < 1:2942182/65‑1 (MQ=255) tctttcttAATATTTTTTCCCAGGAACCTGCCATTAATCACATAGACCAGTTCTTTTCTAATATc < 1:25094/65‑1 (MQ=255) tctttcttAATATCTTTTCTCAGGAACCTGCCATTAATCACATAGACCAGTTCTTTTCTAATATc < 1:2726533/65‑1 (MQ=255) tttAATATCTTTTCTCAGGCACCTGCCATTAATCACATAGACCAGTTCTTTTCTAATATCACGAc < 1:1592184/64‑1 (MQ=255) cttAATATCTTTTCTCAGGACCCTGCCATTAATCACATAGACCAGTTCTTTTCTAATATCACGAc < 1:1681101/65‑1 (MQ=255) cttAATATCTTTTCTCAGGAACCTGCCATTAATCACATAGACCAGTTCTTTTCTAATATCACGAc < 1:1632195/65‑1 (MQ=255) cttAAAATCTTTTCCCAGGACCCTGCCATTAATCACATAGACCAGTTCTTTTCTAATATCACGAc < 1:2830579/65‑1 (MQ=255) ttAATATCTTTTCTCAGGAACCTGCCATTAATCACATAGACCAGTTCTTTTCTAATATCACGACt > 1:834494/1‑65 (MQ=255) tttCTTTCCTCAGGACCCTGCCATTAATCACATAGACCAGTTCTTTTCTAATATCACGACTCAGt < 1:693839/63‑1 (MQ=255) tttCTTTCCCCAGGACCCTGCCATTAATCACATAGACCAGTTCTTTTCTAATATCACGACTCAGt < 1:1575281/63‑1 (MQ=255) tatTTTTTCTCAGGACCCTGCCATTAATCACATAGACCAGTTCTTTTCTAATATCACGACTCAGt < 1:349940/65‑1 (MQ=255) tatTTTTTCACGGGACCCTGCCATTAATCACATAGACCAGTTCTTTTCTAATATCACGACTCAGt < 1:735459/65‑1 (MQ=255) tatCTTTTCTCAGGACCCTGCCATTAATCACATAGACCAGTTCTTTTCTAATATCACGACTCAGt < 1:3368417/65‑1 (MQ=255) tatCTTTTCTCAGGACCCTGCCATTAATCACATAGACCAGTTCTTTTCTAATATCACGACTCAGt < 1:344257/65‑1 (MQ=255) tatCTTTTCTCAGGACCCTGCCATTAATCACATAGACCAGTTCTTTTCTAATATCACGACTCAGt < 1:2716304/65‑1 (MQ=255) tatCTTTTCTCAGGACCCTGCCATTAATCACATAGACCAGTTCTTTTCTAATATCACGACTCAGt < 1:3694145/65‑1 (MQ=255) tatCTTTTCTCAGGAACCTGCCATTAATCACATAGACCAGTTCTTTTCTAATATCACGACTCAGt > 1:1647726/1‑65 (MQ=255) tatCTTTTCTCAGGAACCTGCCATTAATCACATAGACCAGTTCTTTTCTAATATCACGACTCAGt < 1:1703076/65‑1 (MQ=255) tatCTTTTCTCAGAACCCTGCCATTAATCACATAGACCAGTTCTTTTCTAATATCACGACTCAGt < 1:1951409/65‑1 (MQ=255) tatCTTTTCTCAGAACCCTGCCATTAATCACATAGACCAGTTCTTTTCTAATATCACGACTCAGt < 1:1288830/65‑1 (MQ=255) tatCTTTCCTAAGAACCCTGCCATTAATCACATAGACCAGTTCTTTTCTAATATCACGACTCAGt < 1:204610/65‑1 (MQ=255) catCTTTCCTCGGGAACCTGCCATTAATCACATAGACCAGTTCTTTTCTAATATCACGACTCAGt < 1:459169/64‑1 (MQ=255) attCTTTTCTCAGGACCCTGCCATTAATCACATAGACCAGTTCTTTTCTAATATCACGACTCAGt < 1:3722186/63‑1 (MQ=255) tCTTTTCTCAGGAACCTGCCATTAATCACATAGACCAGTTCTTTTCTAATATCACGACTCAGTcc > 1:226543/1‑65 (MQ=255) ttttCTCAGGACCCTGCCATTAATCACATAGACCAGTTCTTTTCTAATATCACGACTCAGTCCAg < 1:608309/65‑1 (MQ=255) ttttCTCAGAACCCTGCCATTAATCACATAGACCAGTTCTTTTCTAATATCACGACTCAGTCCAg < 1:3045630/65‑1 (MQ=255) ttttCCCAGGACCCTGCCATTAATCACATAGACCAGTTCTTTTCTAATATCACGACTCAGTCCAg < 1:3847329/65‑1 (MQ=255) tttCTCAGGGCCCTGCCATTAATCACATAGACCAGTTCTTTTCTAATATCACGACTCAGTCCAGc < 1:2056201/65‑1 (MQ=255) tttCTCAGGACCCTGCCATTAATCACATAGACCAGTTCTTTTCTAATATCACGACTCAGTCCAGc < 1:3257124/65‑1 (MQ=255) tttCTCAGGACCCTGCCATTAATCACATAGACCAGTTCTTTTCTAATATCACGACTCAGTCCAGc < 1:3374785/65‑1 (MQ=255) tttCCCAGGAACCTGCCATTAATCACATAGACCAGTTCTTTTCTAATATCACGACTCAGTCCAGc < 1:138053/65‑1 (MQ=255) tttCACAGGCCCCTGCCATTAATCACATAGACCAGTTCTTTTCTAATATCACGACTCAGTCCAGc < 1:349204/65‑1 (MQ=255) ttcctcGGGACCCTGCCATTAATCACATAGACCAGTTCTTTTCTAATATCACGACTCAGTCCAGc < 1:3182853/62‑1 (MQ=255) ttCTCAGGAACCTGCCATTAATCACATAGACCAGTTCTTTTCTAATATCACGACTCAGTCCAGCg < 1:219130/65‑1 (MQ=255) tctcAGGACCCTGCCATTAATCACATAGACCAGTTCTTTTCTAATATCACGACTCAGTCCAGCGt < 1:3366778/65‑1 (MQ=255) cAGGACCCTGCCATTAATCACATAGACCAGTTCTTTTCTAATATCACGACTCAGTCCAGCGtata < 1:2167170/65‑1 (MQ=255) cAGGAACCTGCCATTAATCACATAGACCAGTTCTTTTCTAATATCACGACTCAGTCCAGCGtata < 1:2160181/65‑1 (MQ=255) ggAACCTGCCATTAATCACATAGACCAGTTCTTTTCTAATATCACGACTCAGTCCAGCGTATAcc > 1:3210277/1‑65 (MQ=255) | ATGCGGATAATTTCCATTTCCCGGTCCGTGATTTTATCTTTCTTAATATCTTTTCTCAGGAACCTGCCATTAATCACATAGACCAGTTCTTTTCTAATATCACGACTCAGTCCAGCGTATACC > NC_000913/310873‑310995 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |