Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 49,791 G→A intergenic (+160/‑32) kefC → / → folA K(+) : H(+) antiporter KefC/dihydrofolate reductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00091349,7910GA100.0% 1793.9 / NA 467intergenic (+160/‑32)kefC/folAK(+) : H(+) antiporter KefC/dihydrofolate reductase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (223/244);  total (223/244)

Only 100 of 522 total aligned reads displayed.

ATATAAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTA  >  NC_000913/49729‑49852
                                                              |                                                             
atatAAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAATg                                                             >  1:3210378/1‑65 (MQ=255)
  ataAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAATGGc                                                           >  1:532235/1‑65 (MQ=255)
     aaaTTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAATGGCGAc                                                        >  1:2842079/1‑65 (MQ=255)
     aaaTTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAATGGCGAc                                                        >  1:3153862/1‑65 (MQ=255)
       aTTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAATGGCGACac                                                      >  1:1492552/1‑64 (MQ=255)
       aTTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAATGGCGACaa                                                      >  1:340860/1‑65 (MQ=255)
       aTTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAATGGCGACaa                                                      >  1:2510575/1‑65 (MQ=255)
       aTTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAATGGCGACaa                                                      >  1:3864758/1‑65 (MQ=255)
       aTTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAAAGGCGACaa                                                      >  1:3821320/1‑65 (MQ=255)
       aTTTTCCTCAACATCATCCTCGCACCACTCGACGACGGTTTACGCTTTACGTATAATGGCGACag                                                      >  1:583067/1‑64 (MQ=255)
                     catcCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAATGGCGACAATTTTTTTTATCggg                                        <  1:329719/65‑1 (MQ=255)
                     catcCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAATGGCGACAATTTTTTTTATCggg                                        <  1:1808239/65‑1 (MQ=255)
                        ccTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAATGGCGACAATTTTTTTTATCGGGaac                                     >  1:2367007/1‑64 (MQ=255)
                        ccTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAATGGCGACAATTTTTTTTATCGGGaaa                                     >  1:23841/1‑65 (MQ=255)
                        ccTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAATGGCGACAATTTTTTTTATCGGGaaa                                     >  1:2434681/1‑65 (MQ=255)
                        ccTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAATGGCGACAATTTTTTTTATCGGGaaa                                     >  1:866063/1‑65 (MQ=255)
                        ccTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAATGGCGACAATTTTTTTTATCGGGaaa                                     >  1:93048/1‑65 (MQ=255)
                         cTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAATGGCGACAA‑TTTTTTTATCGGGAAAtc                                   >  1:172798/1‑65 (MQ=255)
                          tCGCACCAGTCGACGACGGTTTACGCTTTACGTATAATGGCGACAATTTTTTTTATCGGGAAAtc                                   <  1:971431/65‑1 (MQ=255)
                          tCGCACCAGTCGACGACGGTTTACGCTTTACGTATAATGGCGACAATTTTTTTTATCGGGAAAtc                                   <  1:1700888/65‑1 (MQ=255)
                          tCGCACCAGTCGACGACGGTTTACGCTTTACGTATAATGGCGACAATTTTTTTTATCGGGAAAtc                                   >  1:1256262/1‑65 (MQ=255)
                          tCGCACCAGTCGACGACAGTTTACGCTTTACGTATAATGGCGACAATTTTTTTTATCGGGAAAtc                                   <  1:2592883/65‑1 (MQ=255)
                           cGCACCAGTCGACGACGGTTTACGCTTTACGTATAATGGCGACAATTTTTTTTATCGGGAAAtct                                  <  1:452796/65‑1 (MQ=255)
                           cGCACCAGTCGACGACGGTTTACGCTTTACGTATAATGGCGACAA‑TTTTTTTATCGGGAAAtctc                                 <  1:3367343/65‑1 (MQ=255)
                            gCACCAGTCGACGACGGTTTACGCTTTACGTATAATGGCGACAATTTTTTTTATCGGGAAAtctc                                 <  1:684205/65‑1 (MQ=255)
                            gCACCAGTCGACGACGGTTTACGCTTTACGTATAATGGCGACAATTTTTTTTATCGGGAAAtctc                                 <  1:1976684/65‑1 (MQ=255)
                            gCACCAGTCGACGACGGTTTACGCTTTACGTATAATGGCGACAATTTTTTTTATCGGGAAAtctc                                 <  1:3250811/65‑1 (MQ=255)
                             cACCAGTCGACGACGGTTTACGCTTTACGTATAATGGCGACAATTTTTTTTATCGGGAAATCTCa                                >  1:849768/1‑65 (MQ=255)
                             cACCAGTCGACGACGGTTTACGCTTTACGTATAATGGCGACAATTTTTTTTATCGGGAAATCTCa                                >  1:658821/1‑65 (MQ=255)
                              aCCAGTCGACGACGGTTTACGCTTTACGTATAATGGCGACAATTTTTTTTATCGGGAAATCTCaa                               >  1:2628958/1‑65 (MQ=255)
                              aCCAGTCGACGACGGTTTACGCTTTACGTATAATGGCGACAATTTTTTTTATCGGGAAATCTCaa                               >  1:3764313/1‑65 (MQ=255)
                                 aGTCGACGACGGTTTACGCTTTACGTATAATGGCGACAATTTTTTTTATCGGGAAATCTCAATGa                            <  1:3034437/65‑1 (MQ=255)
                                 aGTCGACGACGGTTTACGCTTTACGTATAATGGCGACAATTTTTTTTATCGGGAAATCTCAATGa                            <  1:2303269/65‑1 (MQ=255)
                                 aGTCGACGACGGTTTACGCTTTACGTATAATGGCGACAATTTTTTTTATCGGGAAATCTCAATGa                            <  1:291049/65‑1 (MQ=255)
                                  gTCGACGACGGTTTACGCTTTACGTATAATGGCGACACTTTTTTTTATCGGGAAATCTCAATGAt                           >  1:620179/1‑65 (MQ=255)
                                   tCGACGACGGTTTACGCTTTACGTATAATGGCGACAATTTTTTTTATCGGGAAATCTCAATGATc                          <  1:3200184/65‑1 (MQ=255)
                                    cgacgaCGGTTTACGCTTTACGTATAATGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCa                         >  1:2208864/1‑65 (MQ=255)
                                    cgacgaCGGTTTACGCTTTACGTATAATGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCa                         <  1:3515047/65‑1 (MQ=255)
                                    cgacgaCGGTTTACGCTTTACGTATAATGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCa                         >  1:2024692/1‑65 (MQ=255)
                                    cgacgaCGGTTTACGCTTTACGTATAATGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCa                         >  1:2771959/1‑65 (MQ=255)
                                      gcgacgGTTTACGCTTTACGTATAATGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGt                       <  1:364288/64‑1 (MQ=255)
                                      acgacgGTTTACGCTTTACGTATAATGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGt                       <  1:1005570/65‑1 (MQ=255)
                                      acgacgGTTTACGCTTTACGTATAATGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGt                       <  1:2924371/65‑1 (MQ=255)
                                      acgacgGTTTACGCTTTACGTATAATGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGt                       <  1:409530/65‑1 (MQ=255)
                                      acgacgGTTTACGCTTTACGTATAATGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGt                       <  1:3466055/65‑1 (MQ=255)
                                      acgacgGTTTACGCTTTACGTATAATGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGt                       <  1:3556594/65‑1 (MQ=255)
                                      acgacgGTTTACGCTTTACGTATAATGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGt                       >  1:3694872/1‑65 (MQ=255)
                                      acgacgGTTTACGCTTTACGTATAATGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGt                       <  1:3729033/65‑1 (MQ=255)
                                      acgacgGTTTACGCTTTACGTATAATGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGt                       <  1:2880226/65‑1 (MQ=255)
                                      acgacgGTTTACGCTTTACGTATAATGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGt                       <  1:190301/65‑1 (MQ=255)
                                      acgacgGTTTACGCTTTACGTATAATGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGt                       <  1:2136043/65‑1 (MQ=255)
                                      acgacgGTTTACGCTTTACGTATAATGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGt                       <  1:1458943/65‑1 (MQ=255)
                                      acgacgGTTTACGCTTTACGTATAATGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGt                       <  1:2157746/65‑1 (MQ=255)
                                      acgacgGTTTACGCTTTACGTATAATGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGt                       <  1:1523903/65‑1 (MQ=255)
                                      acgacgGTTTACGCTTTACGTATAATGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGt                       <  1:2236884/65‑1 (MQ=255)
                                      acgacgGTTTACGCTTTACGTATAATGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGt                       <  1:2470578/65‑1 (MQ=255)
                                      acgacgGTTTACGCTTTACGTATAATGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGt                       <  1:1169896/65‑1 (MQ=255)
                                        gacgGTTTACGCTTTACGTATAATGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCt                     >  1:3323171/1‑65 (MQ=255)
                                         acgGTTTACGCTTTACGTATAATGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTg                    >  1:903962/1‑65 (MQ=255)
                                         acgGTTTACGCTTTACGTATAATGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTg                    >  1:812562/1‑65 (MQ=255)
                                         acgGTTTACGCTTTACGTATAATGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTg                    >  1:1845859/1‑65 (MQ=255)
                                         acgGTTTACGCTTTACGTATAATGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTg                    <  1:3274968/65‑1 (MQ=255)
                                         acgGTTTACGCTTTACGTATAATGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTg                    >  1:56202/1‑65 (MQ=255)
                                          cgGTTTACGCTTTACGTATAATGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGa                   <  1:3446810/65‑1 (MQ=255)
                                           ggTTTACGCTTTACGTATAATGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGAt                  >  1:1657543/1‑65 (MQ=255)
                                             tttACGCTTTACGTATAATGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTg                >  1:1406346/1‑65 (MQ=255)
                                                 cGCTTTACGTATAATGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTtcggc            >  1:1316655/1‑65 (MQ=255)
                                                 cGCTTTACGTATAATGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTgctgt            >  1:3078628/1‑62 (MQ=255)
                                                 cGCTTTACGTATAATGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTgcggc            <  1:712537/65‑1 (MQ=255)
                                                  gCTTTACGTATCATGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTgcggcg           >  1:2615178/1‑65 (MQ=255)
                                                  gCTTTACGTATAATGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTgcggcg           >  1:2089364/1‑65 (MQ=255)
                                                  gCTTTACGTATAATGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTgcggcg           >  1:394841/1‑65 (MQ=255)
                                                  gCTTTACGTATAATGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTgcggcg           >  1:49647/1‑65 (MQ=255)
                                                  gCTTTACGTATAATGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTgcggcg           >  1:1610417/1‑65 (MQ=255)
                                                  gCTTTACGTATAATGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTgcggcg           >  1:3231572/1‑65 (MQ=255)
                                                  gCTTTACGTATAATGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTgcggcg           >  1:2685215/1‑65 (MQ=255)
                                                  gCTTTACGTATAATGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTgcggcg           >  1:2966500/1‑65 (MQ=255)
                                                  gCTTTACGTATAATGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTgcggcg           >  1:2795311/1‑65 (MQ=255)
                                                   cTTTACGTATAATGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGt          <  1:828167/65‑1 (MQ=255)
                                                   cTTTACGTATAATGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGt          <  1:1147719/65‑1 (MQ=255)
                                                   cTTTACGTATAATGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGt          <  1:3841974/65‑1 (MQ=255)
                                                   cTTTACGTATAATGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGt          <  1:2537989/65‑1 (MQ=255)
                                                   cTTTACGTATAATGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGt          >  1:3100843/1‑65 (MQ=255)
                                                   cTTTACGTATAATGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGt          <  1:1114438/65‑1 (MQ=255)
                                                   cTTTACGTATAATGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGt          <  1:1232910/65‑1 (MQ=255)
                                                       aCGTATAATGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGc      <  1:1131134/65‑1 (MQ=255)
                                                       aCGTATAATGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGc      <  1:1063098/65‑1 (MQ=255)
                                                       aCGTATAATGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGc      <  1:954168/65‑1 (MQ=255)
                                                       aCGTATAATGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGc      <  1:1354665/65‑1 (MQ=255)
                                                       aCGTATAATGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGc      <  1:1562697/65‑1 (MQ=255)
                                                       aCGTATAATGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGc      <  1:1678381/65‑1 (MQ=255)
                                                       aCGTATAATGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGc      <  1:1837100/65‑1 (MQ=255)
                                                       aCGTATAATGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGc      <  1:205994/65‑1 (MQ=255)
                                                       aCGTATAATGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGc      <  1:3040305/65‑1 (MQ=255)
                                                       aCGTATAATGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGc      <  1:2998755/65‑1 (MQ=255)
                                                       aCGTATAATGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGc      <  1:2709341/65‑1 (MQ=255)
                                                        cGTATAATGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCg     >  1:751177/1‑65 (MQ=255)
                                                        cGTATAATGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCg     >  1:3706633/1‑65 (MQ=255)
                                                           ataATGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTa  <  1:3624003/65‑1 (MQ=255)
                                                           ataATGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTa  <  1:227966/65‑1 (MQ=255)
                                                              |                                                             
ATATAAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTA  >  NC_000913/49729‑49852

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑