| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,003,829 | C→A | T114N (ACC→AAC) | xdhC → | putative xanthine dehydrogenase iron‑sulfur‑binding subunit XdhC |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,003,829 | 0 | C | A | 77.8% | 14.2 / 2.1 | 9 | T114N (ACC→AAC) | xdhC | putative xanthine dehydrogenase iron‑sulfur‑binding subunit XdhC |
| Reads supporting (aligned to +/- strand): ref base C (0/2); new base A (7/0); total (7/2) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.78e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.02e-01 | |||||||||||
GCTTATGCGAAATCCGGCGCAGTGCAGTGCGGGTTTTGTACGCCTGGCCTGATTATGGCTACCACGGCAATGCTGGCG‑AAACCACGCGAGAAGCCATTAACCATTACGGAAATTCGTCGCGGACTG > NC_000913/3003768‑3003893 | gCTTATGCGAAATCCGGCGCAGTGCAGTGCGGGTTTTGTACGCCTGGCCTGATTATGGCTAcaaa > 1:812963/1‑62 (MQ=255) cTTATGCGAAATCCGGCGCAGTGCAGTGCGGGTTTTGTACGCCTGGCCTGATTATGGCTACCACg < 1:3800168/65‑1 (MQ=255) gCGAAATCCGGCGCAGTGCAGTGCGGGTTTTGTACGCCTGGCCTGATTATGGCTACCACGGCaca > 1:327375/1‑63 (MQ=255) gCGAAATCCGGCGCAGTGCAGTGCGGGTTTTGTACGCCTGGCCTGATTATGGCTACCACGGCaaa > 1:219534/1‑64 (MQ=255) gCGAAATCCGGCGCAGTGCAGTGCGGGTTTTGTACGCCTGGCCTGATTATGGCTAACACGGCAAt > 1:1299278/1‑65 (MQ=255) gCGAAATCCGGCGCAGTGCAGTGCGGGTTTTGTACGCCTGGCCTGATTATGGCTAAAAAGGCaag > 1:2255654/1‑64 (MQ=255) aaaTCCGGCGCAGTGCAGTGCGGGTTTTGTACGCCTGGCCTGATTATGGCTAACAAGGGAATgac > 1:408073/1‑63 (MQ=255) tCCGGCGCAGTGCAGTGCGGGTTTTGTACGCCTGGCCTGATTATGGCTAACACGGCAATGCtcgg > 1:1510401/1‑62 (MQ=255) gtgcagtgcGGGTTTTGTACGCCTGGCCTGATTATGGCTACCACGGCAATGCTGGCG‑AAACCAcg < 1:95702/65‑1 (MQ=255) gcagtgcGGGTTTTGTACGCCTGGCCTGATTATGGCTAACACAGCAATTCTGGGG‑GAACCAccag > 1:3160234/1‑62 (MQ=255) agtgcGGGTTTTGTACGCCTGGCCTGATTATGGCTACTACAGCAAACGAGGCG‑CAACCACCCgag > 1:1468553/1‑65 (MQ=255) agtgcGGGTTTTGTACGCCTGGCCTGATTATGGCTAACACGGCAAAGCTGGGG‑GAACCACGCgag > 1:388988/1‑65 (MQ=255) agtgcGGGTTTTGTACGCCTGGCCTGATTATGGCTAAAACGGCAAAGGTGGGG‑GAAACAcgcggg > 1:1381562/1‑63 (MQ=255) gcGGGTTTTGTACGCCTGGCCTGATTATGGCTAACACGGGAATGCTGGGG‑AAAACACGCCAGGAg > 1:2368390/1‑65 (MQ=255) gcGGGTTTTGTACGCCTGGCCTGATTATGGCTAACACGGCCATGCTGGCG‑GAACCACGCGGGAAg > 1:2368214/1‑65 (MQ=255) gcGGGTTTTGTACGCCTGGCCTGATTATGGCTAACACCGCAATGCTGGCGTAAACTACCCGGGaa > 1:2763661/1‑65 (MQ=255) gggTTTTGTACGCCTGGCCTGATTATGGCTAACAAGGCAAAGCTGGCG‑GAACCACGCGAGAAGcc > 1:638238/1‑65 (MQ=255) ttttGTACGCCTGGCCTGATTATGGCTAACAACGCCAAGCCGGCG‑GAAACACCCGAGGAGCCact > 1:2979417/1‑63 (MQ=255) tACGCCTGGCCTGATTATGGCTAACACGGCAAGGGTGGCG‑GAAACACGCGAGAAGCCATTAAGCa > 1:844860/1‑65 (MQ=255) tACGCCTGGCCTGATTATGGCTAACACCGCAATGCTGGTG‑AAACCACCCGAGAAAGCAATAAcac > 1:3586940/1‑63 (MQ=255) ctggccttATTAAGCCTCCCACGGCAATGCTGGCG‑AACCCACGCGAGAAGCCATTAACCATTACg < 1:3551300/65‑1 (MQ=255) ttcaTGGCTACCACGGCAATGCTGGCG‑AAACCACGCGAGAAGCCATTAACCATTACGGAAATtcg < 1:2184324/62‑1 (MQ=255) ctcccACGGAAAGGGTGGCG‑AACCCGCGCAAGAAGCCATTAACCATTACGGAAATTCGTCGCGGa < 1:1449900/62‑1 (MQ=255) ccACGGCAATGCTGGCG‑AAACCACGCGAGAAGCCATTAACCATTACGGAAATTCGTCGCGGACTg > 1:787212/1‑65 (MQ=255) | GCTTATGCGAAATCCGGCGCAGTGCAGTGCGGGTTTTGTACGCCTGGCCTGATTATGGCTACCACGGCAATGCTGGCG‑AAACCACGCGAGAAGCCATTAACCATTACGGAAATTCGTCGCGGACTG > NC_000913/3003768‑3003893 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |