Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,390,754	G→T	A252A (GCC→GCA)	rsgA ←	ribosome small subunit‑dependent GTPase A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,390,754	0	G	T	100.0%	118.7 / NA	33	A252A (GCC→GCA)	rsgA	ribosome small subunit‑dependent GTPase A
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (24/9);  total (24/9)

CTCACGCACTCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGTGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGATCTC  >  NC_000913/4390694‑4390813                                                             |                                                            cTCACGCACTCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGctgcgg                                                         >  1:1870301/1‑65 (MQ=255)  tCACGCACTCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGt                                                        <  1:2489804/65‑1 (MQ=255)  tCACGCACTCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGt                                                        <  1:2040224/65‑1 (MQ=255)  tCACGCACTCCGGGGGAGTCAATCACATCCCCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGt                                                        <  1:2610137/65‑1 (MQ=255)     cGCACTCGCGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGTCgt                                                     <  1:847761/65‑1 (MQ=255)      gCACTCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCTGCTGTctct                                                    >  1:3592763/1‑62 (MQ=255)      gCACTCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGTCgtg                                                    >  1:389192/1‑65 (MQ=255)      gCACTCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGTCgtg                                                    >  1:3660775/1‑65 (MQ=255)        aCTCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGTCgtggt                                                  >  1:3580539/1‑65 (MQ=255)         cTCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGTCgtggtg                                                 <  1:2253204/65‑1 (MQ=255)         cTCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGTCgtggtg                                                 <  1:2898945/65‑1 (MQ=255)          tCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGgtgt                                                <  1:2838965/65‑1 (MQ=255)                  aGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGGTGTGCTGAcct                                        >  1:312761/1‑64 (MQ=255)                  aGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGGTGTGCTGACCg                                        >  1:1474487/1‑65 (MQ=255)                  aGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGGTGTGCTGACCg                                        >  1:3286202/1‑65 (MQ=255)                  aGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGGTGTGCTGACCg                                        >  1:418547/1‑65 (MQ=255)                  aGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGGTGTGCTGACCg                                        >  1:3214343/1‑65 (MQ=255)                  aGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGGTGTGCTGACCg                                        >  1:835833/1‑65 (MQ=255)                  aGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGGTGTGCTGACCg                                        >  1:2509439/1‑65 (MQ=255)                  aGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGGTGTGCTGACCg                                        >  1:1052274/1‑65 (MQ=255)                  aGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGGTGTGCTGACCg                                        >  1:1967947/1‑65 (MQ=255)                  aGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGGTGTGCTGACCg                                        >  1:1580344/1‑65 (MQ=255)                  aGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGGTGGGCTGACCg                                        >  1:1493865/1‑65 (MQ=255)                  aGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGTCGGGGTGTGCTGACCg                                        >  1:3438554/1‑65 (MQ=255)                    tCAATCCCAGCCCCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGGTGTGCTGACCGAg                                      <  1:2813454/65‑1 (MQ=255)                       atcgcatcacCGCGGTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGGTGTGCTGACCGAGccc                                   <  1:3283674/65‑1 (MQ=255)                                  gccgTGCGGGAAGTGATACAGCCGGGCTGCGGTCGTGGGGTGCTGACCGAGCCCCGAGTCGTCag                        >  1:2338004/1‑65 (MQ=255)                                   ccgTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGGTGTGCTGACCGAGCCCCGAGTTGTCaga                       <  1:2325466/65‑1 (MQ=255)                                               ggATAAAGCCGAGCTGCGGTCGTGGTGTGCGGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGt           <  1:2671602/64‑1 (MQ=255)                                                   aCAGCCGAGCTGCGGTCGTGGTGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAgg       >  1:3130976/1‑65 (MQ=255)                                                   aCAGCCGAGCTGCGGTCGTGGTGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAgg       >  1:3344119/1‑65 (MQ=255)                                                   aCAGCCGAGCTGCGGTCGTGGTGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAgg       >  1:634546/1‑65 (MQ=255)                                                      gCCGAGCTGCGGTCGTGGTGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGAtc    >  1:922505/1‑65 (MQ=255)                                                      gCCGAGCTGCGGTCGTGGTGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGAtc    >  1:3782979/1‑65 (MQ=255)                                                      gCCGAGCTGCGGTCGTGGTGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGAtc    >  1:2131713/1‑65 (MQ=255)                                                      gCCGAGCTGCGGTCGTGGGGTGCTGACCGAGCCCCGAGTTGTCAGAGATACGGTTGCTCAGGAtc    >  1:2264825/1‑65 (MQ=255)                                                        cGAGCTGCGGTCGTGGTGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGAtctc  >  1:2808265/1‑65 (MQ=255)                                                             |                                                            CTCACGCACTCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGTGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGATCTC  >  NC_000913/4390694‑4390813

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑