Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,560,455	+G	pseudogene (151/758 nt)	glpR ←	DNA‑binding transcriptional repressor GlpR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,560,455	1	.	G	100.0%	60.7 / NA	19	pseudogene (151/758 nt)	glpR	DNA‑binding transcriptional repressor GlpR
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base G (14/5);  total (14/5)

CTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑C‑CAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCT  >  NC_000913/3560394‑3560512                                                                |                                                          cTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑cgca                                                         >  1:774712/1‑62 (MQ=255)    gCGGTCGTGCCCCGGGGTGTTAACCGAACGGGAAGGCAGGGCCGCCCCGCCATGATGG‑CGCAGGa                                                      <  1:3067235/65‑1 (MQ=255)             ccACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGGATCAGGtttt                                             >  1:3467713/1‑65 (MQ=255)              cACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGGATCAGGTTTTg                                            >  1:1329324/1‑65 (MQ=255)                   cGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCg                                       >  1:1089204/1‑65 (MQ=255)                       ttAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAg                                   <  1:2115425/65‑1 (MQ=255)                        tAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGc                                  <  1:746171/65‑1 (MQ=255)                        tAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGc                                  >  1:2527409/1‑65 (MQ=255)                         atccGAACTGGAAGGCGGCGCCGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCt                                 <  1:533242/63‑1 (MQ=38)                         aaCCGAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCt                                 >  1:3163335/1‑65 (MQ=255)                           ccGAACTGGAAGGCAGCGCCGCGCCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCa                               >  1:730875/1‑65 (MQ=39)                           ccGAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCa                               >  1:3103616/1‑65 (MQ=255)                           ccGAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCa                               >  1:679302/1‑65 (MQ=255)                           ccGAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCa                               <  1:605959/65‑1 (MQ=255)                               aCTGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGa                           >  1:3233913/1‑65 (MQ=255)                                      ggCAGCGCCGCACCGCCATGATGGCC‑CAGGATCAGGCTTGGATCCGCCAGCTCATTGAGGTcgcg                    >  1:156305/1‑65 (MQ=255)                                       gCAGCGCCGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCgcgg                   >  1:2271694/1‑65 (MQ=255)                                       gCAGCGCCGCACCGCCATGATGG‑CGCAGGACCAGGCTTTGCTCCGCCAGCTCATTGAGGTCgcgg                   >  1:2969914/1‑65 (MQ=255)                                            gccgcACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAAt              >  1:1584594/1‑65 (MQ=255)                                                 aCCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCt         >  1:2243534/1‑65 (MQ=255)                                                      cATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCggg    >  1:2392429/1‑65 (MQ=255)                                                        tgatgG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCt  >  1:3027524/1‑65 (MQ=255)                                                                |                                                          CTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑C‑CAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCT  >  NC_000913/3560394‑3560512

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑