| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 151,656 | 0 | T | G | 26.1% | 86.4 / 32.5 | 46 | A192A (GCA→GCC) | yadL | putative fimbrial protein YadL |
| Reads supporting (aligned to +/- strand): ref base T (0/34); new base G (12/0); total (12/34) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.57e-11 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.40e-01 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
ATGCATATATAGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAG‑AAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGG > NC_000913/151594‑151713 | aTGCATATATAGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGc < 1:542322/65‑1 (MQ=255) tataGTTCCTGAATCTGTACGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGGGGAG‑gaaaa > 1:831163/1‑64 (MQ=255) ataGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGGGGGG‑aaaaac > 1:1894864/1‑63 (MQ=255) ataGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGGGGAG‑aaaatg > 1:1985850/1‑63 (MQ=255) ataGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGGGCAG‑gaaaat > 1:2795637/1‑63 (MQ=255) taGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGGGGAG‑AAAAAtt > 1:2904914/1‑65 (MQ=255) taGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGGGGAG‑AAAAAtt > 1:2178755/1‑65 (MQ=255) taGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGGGCAG‑AAAACtt > 1:421518/1‑65 (MQ=255) aGTTCCTGAATCTGTAAGTCTGTTCGTTATAGGTTACTGTAAATGTGGCTGGGGGAG‑AAAAATTa > 1:1291065/1‑65 (MQ=255) aGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGGGGGG‑AAAACATa > 1:3121905/1‑65 (MQ=255) aGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGGGGGG‑AAAAATTa > 1:1241187/1‑65 (MQ=255) aGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGGGGCG‑AAAAcgtt > 1:1454553/1‑62 (MQ=255) aGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGGGGAG‑AAAACTTa > 1:3148263/1‑65 (MQ=255) aGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGGGGAG‑AAAAATTa > 1:389933/1‑65 (MQ=255) aGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGGGGAG‑AAAAATTa > 1:891952/1‑65 (MQ=255) aGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGGGCGG‑GAAAATTa > 1:130613/1‑65 (MQ=255) aGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGGGCGG‑AAAACTTa > 1:2597095/1‑65 (MQ=255) aGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGGGCCG‑AGAAATTa > 1:3157829/1‑65 (MQ=255) aGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGGGCAG‑AAAACTTa > 1:312791/1‑65 (MQ=255) aGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGGGCAG‑AAAACATa > 1:223012/1‑65 (MQ=255) aGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGGGCAG‑AAAAATTa > 1:170690/1‑65 (MQ=255) aGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGGGCAG‑AAAAATTa > 1:3450602/1‑65 (MQ=255) ccTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAG‑AAAACTTACCCg < 1:2684273/65‑1 (MQ=255) tGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAG‑AAAACTTACCCGCa < 1:3207302/65‑1 (MQ=255) tGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAG‑AAAACTTACCCGCa < 1:2957732/65‑1 (MQ=255) tGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAG‑AAAACTTACCCGCa < 1:1636184/65‑1 (MQ=255) aaTCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAG‑AAAACTTACCCGCAGt < 1:3447427/65‑1 (MQ=255) tGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAG‑AAAACTTACCCGCAGTGACa < 1:1938527/65‑1 (MQ=255) aaGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAG‑AAAACTTACCCGCAGTGACATTg < 1:2535961/65‑1 (MQ=255) aGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAG‑AAAACTTACCCGCAGTGACATTGt < 1:2902925/65‑1 (MQ=255) aGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAG‑AAAACTTACCCGCAGTGACATTGt < 1:1935926/65‑1 (MQ=255) aGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAG‑AAAACTTACCCGCAGTGACATTGt < 1:1073693/65‑1 (MQ=255) aGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAG‑AAAACTTACCCGCAGTGACATTGt < 1:1855690/65‑1 (MQ=255) tCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGGGCGG‑AAAACTTACCCGCAGTGACATTGttt > 1:2921130/1‑65 (MQ=255) tCTGTTAGTTATAGGCTACTGTAAATGTGGCTGGGGCGGCAAAACTAACCC‑CTATGGCATTGttt > 1:368797/1‑65 (MQ=255) tagttaTAGGTCACTGTAAATGTGGCTGGTGCAG‑AAAACTTACCCGCAGTGACATTGTTTACGGt < 1:1491952/65‑1 (MQ=255) ggTTACTGTAAATGTGGCTGGGGGAG‑GAAAATTACCCCCAGGGACATTGTTTTTGGGTttttttc > 1:1054032/1‑64 (MQ=255) ggTTACTGTAAATGTGGCTGGGGGAG‑AAAACTTACCCGCCGGGACCTTTTTTTCGGGtttttttt > 1:1733518/1‑65 (MQ=255) ggTTACTGTAAATGTGGCTGGGGGAG‑AAAACTTACCCCCAGGGACATTGTTTTTGGTtttttttt > 1:2782509/1‑65 (MQ=255) ggTTACTGTAAATGTGGCTGGGGCGG‑AAAAGATAACACCCGGGACATTGTTTGCGGTTTTAtttt > 1:2915764/1‑65 (MQ=255) gTTACTGTAAATGTGGCTGGGGGAG‑AAAACTTACCCCCAGCGAAATTGTTTTACGtttttttttt > 1:3410956/1‑65 (MQ=255) gTTACTGTAAATGTGGCTGGGGGAG‑AAAAATTACCCGCAGGGACATTGTTTTCGGGTtttttttt > 1:1064893/1‑65 (MQ=255) gTTACTGTAAATGTGGCTGGGGCAG‑AAAACATACCCCCAGTGGCATTGTTTTTGGGTtttttttt > 1:826921/1‑65 (MQ=255) tGTAAATGTGGCTGGGGGAG‑GAAAATTACCCGCGGGGACATTTTTTTCGGTTTTTTTTTTTTTCa > 1:3210227/1‑65 (MQ=255) tGTAAATGTGGCTGGGGGAG‑AAAACTTACCCGCAGGGGCATTGTTTTCGGTTTTTTTTTTTTTCa > 1:932173/1‑65 (MQ=255) gTAAATGTGGCTGGGGCAG‑AAAACTTACCCGCCGTGACATTGTTTTCGGTTTTATTTTTTTCCAc > 1:40576/1‑65 (MQ=255) aaaTGTGGCTGGTGCAG‑AAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGa < 1:404808/65‑1 (MQ=255) aaaTGTGGCTGGTGCAG‑AAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGa < 1:423376/65‑1 (MQ=255) aaaTGTGGCTGGTGCAG‑AAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGa < 1:979576/65‑1 (MQ=255) aaaTGTGGCTGGTGCAG‑AAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGa < 1:2299773/65‑1 (MQ=255) aaaTGTGGCTGGTGCAG‑AAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGa < 1:2301079/65‑1 (MQ=255) aaaTGTGGCTGGTGCAG‑AAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGa < 1:2421753/65‑1 (MQ=255) tgtgGCTGGGGGAG‑AAAACTTACCCGCCGTGGAATTGTTTTCGGGTTTTTTTTTTTTCAccacca > 1:1146172/1‑65 (MQ=255) tgtgGCTGGGGCAG‑AAAACTTACCCCCAGCGGCATTTTTTTTGGGTTTTTTTTTTTTCACGACCa > 1:1249111/1‑65 (MQ=255) ttgCTGGTGCAG‑AAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAgg < 1:2334961/63‑1 (MQ=255) tgGCTGGTGCAG‑AAAGCTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAgg < 1:1822113/65‑1 (MQ=255) tgGCTGGTGCAG‑AAAACTTCCCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAgg < 1:2161811/65‑1 (MQ=255) tgGCTGGTGCAG‑AAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAgg < 1:3195169/65‑1 (MQ=255) tgGCTGGTGCAG‑AAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAgg < 1:2740256/65‑1 (MQ=255) tgGCTGGTGCAG‑AAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAgg < 1:1187589/65‑1 (MQ=255) tgGCTGGTGCAG‑AAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAgg < 1:1490514/65‑1 (MQ=255) tgGCTGGTGCAG‑AAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAgg < 1:72194/65‑1 (MQ=255) tgGCTGGTGCAG‑AAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAgg < 1:63037/65‑1 (MQ=255) tgGCTGGTGCAG‑AAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAgg < 1:2029956/65‑1 (MQ=255) tgGCTGGTGCAG‑AAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAgg < 1:2199038/65‑1 (MQ=255) tgGCTGGTGCAG‑AAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAgg < 1:3113032/65‑1 (MQ=255) tgGCTGGTGCAG‑AAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAgg < 1:3005789/65‑1 (MQ=255) tgGCTGGTGCAG‑AAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAgg < 1:2331017/65‑1 (MQ=255) tgGCTGGTGCAG‑AAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAgg < 1:3468715/65‑1 (MQ=255) tgGCTGGTGCAG‑AAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAgg < 1:2366961/65‑1 (MQ=255) tgGCTGGTGCAG‑AAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAgg < 1:3303848/65‑1 (MQ=255) | ATGCATATATAGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAG‑AAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGG > NC_000913/151594‑151713 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |