| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,856,645 | 0 | T | G | 25.8% | 60.3 / 11.7 | 31 | K54N (AAA→AAC) | ygbA | protein YgbA |
| Reads supporting (aligned to +/- strand): ref base T (8/15); new base G (8/0); total (16/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.45e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TCTGTTTCATCTCTTCACGCTTTGCAGGCTGGTAACAGTGCACCGGACACTGCTTACAGGCTGGTTTCTCTTCGCCGAACACACATTTATCCAGCCGCTTTTGCGCGTAAACAAACAACGCCTC > NC_000913/2856581‑2856704 | tCTGTTTCATCTCTTCACGCTTTGCAGGCTGGTAACAGTGCACCGGACACTGCTTACGGGCTGGt > 1:1792378/1‑65 (MQ=255)tCTGTTTCATCTCTTCACGCTTTGCAGGCTGGTAACAGTGCACCGGACACTGCTTACAGGCTGGt > 1:656873/1‑65 (MQ=255)tCTGTTTCATCTCTTCACGCTTTGCAGGCTGGTAACAGTGCACCGGACACTGCTTACAGGCTGGt > 1:2206128/1‑65 (MQ=255) tttCATCTCTTCACGCTTTGCAGGCTGGTAACAGTGCACCGGACACTGCTTACAGGCTGGGTtct > 1:3016799/1‑65 (MQ=255) tctcTTCACGCTTTGCAGGCTGGTAACAGTGCACCGGACACTGCTTACAGGCTGGTTTCTCtttg > 1:2456189/1‑63 (MQ=255) ctctTCACGCTTTGCAGGCTGGTAACAGTGCACCGGACACTGCTTACAGGCTGGTTTCTCTTCGc < 1:1022938/65‑1 (MQ=255) ctctTCACGCTTTGCAGGCTGGTAACAGTGCACCGGACACTGCTTACAGGCTGGGTTTTTTTCGc > 1:1906140/1‑65 (MQ=255) ttCACGCTTTGCAGGCTGGTAACAGTGCACCGGACACTGCTTACAGGCTGGTTTCTCTTCGCCGa < 1:1801188/65‑1 (MQ=255) cACGCTTTGCAGGCTGGTAACAGTGCACCGGACACTGCTTACAGGCTGGTTTCTCTTCGCCGAac < 1:2288583/65‑1 (MQ=255) cACGCTTTGCAGGCTGGTAACAGTGCACCGGACACTGCTTACAGGCTGGTTTCTCTTCGCCGAac < 1:345831/65‑1 (MQ=255) cACGCTTTGCAGGCTGGTAACAGTGCACCGGACACTGCTTACAGGCTGGTTTCTCTTCGCCGAac < 1:1290179/65‑1 (MQ=255) gCTTTGCAGGCTGGTAACAGTGCACCGGACACTGCTTACAGGCTGGGTTTTCTTCGCCGGacaca > 1:1703517/1‑65 (MQ=255) cTTTGCAGGCTGGTAACAGTGCACCGGACACTGCTTACAGGCTGGTTTCTCTTCGCCGAacacac < 1:2736452/65‑1 (MQ=255) tttGCAGGCTGGTAGCAGTGCGCCGGACACTGCTTACAGGCTGGTTTCTCTTCGCCGAAcacaca < 1:1090484/65‑1 (MQ=255) tttGCAGGCTGGTAACAGTGCACCGGACACTGCTTACAGGCTGGTTTCTCTTCGCCGAAcacaca < 1:307258/65‑1 (MQ=255) tttGCAGGCTGGTAACAGTGCACCGGACACTGCTTACAGGCTGGTTTCTCTTCGCCGAAcacaca < 1:2873914/65‑1 (MQ=255) tttGCAGGCTGGTAACAGTGCACCGGACACTGCTTACAGGCTGGTTTCTCTTCGCCGAAcacaca < 1:2744781/65‑1 (MQ=255) tttGCAGGCTGGTAACAGTGCACCGGACACTGCTTACAGGCTGGTTTCTCTTCGCCGAAcacaca < 1:505168/65‑1 (MQ=255) tttGCAGGCTGGTAACAGTGCACCGGACACTGCTTACAGGCTGGTTTCTCTTCGCCGAAcacaca < 1:530147/65‑1 (MQ=255) tttGCAGGCTGGTAACAGTGCACCGGACACTGCTTACAGGCTGGTTTCTCTTCGCCGAAcacaca < 1:2412795/65‑1 (MQ=255) tttGCAGGCTGGTAACAGTGCACCGGACACTGCTTACAGGCTGGTTTCTCTTCGCCGAAcacaca < 1:725950/65‑1 (MQ=255) aCAGTGCACCGGACACTGCTTACAGGCTGGTTTTTCTTCTCCCAACACCCACTTATCCAGCCGCt > 1:3194846/1‑65 (MQ=255) aCAGTGCACCGGACACTGCTTACAGGCTGGGTTTTCTTCGCCGAAACCACATTTATCCAGccccc > 1:2347417/1‑62 (MQ=255) aCAGTGCACCGGACACTGCTTACAGGCTGGGTTCTCTTCCCCGGACACACATTTATCCAGCCCCt > 1:1171416/1‑65 (MQ=255) gTGCACCGGACACTGCTTACAGGCTGGTTTCTCTTCGCCGCACACACCTTTATCCAGCCGCttct > 1:2083592/1‑63 (MQ=255) gTGCACCGGACACTGCTTACAGGCTGGGTTCTCTTTGCCGAACACACATTTATCCAGCCGCtttt > 1:1314066/1‑65 (MQ=255) gTGCACCGGACACTGCTTACAGGCTGGGTTCTCTTTGCCGAACACACATTTATCCAGCCGCtttt > 1:329802/1‑65 (MQ=255) gTGCACCGGACACTGCTTACAGGCTGGGTTCTCTTCGCCGAACACACATTTATCCAGCCGCtttt > 1:1453876/1‑65 (MQ=255) aCCGGACACTGCTTACAGGCTGGTTTCTCTTCGCCGAACACACATTTATCCAACCCCTTTTtcgc > 1:1731742/1‑65 (MQ=255) ggACACTGCTTACAGGCTGGTTTCTCTTCGCCGAACACACCTTTATCCAGCCGCTTTTGCGCGTa > 1:41439/1‑65 (MQ=255) acacTGCTTACAGGCTGGTTTCTCTTCGCCGAACACACATTTATCCAGCCGCTTTTTCGCGTaaa > 1:1978682/1‑65 (MQ=255) acacTGCTTACAGGCTGGTTTCTCTTCGCCGAACACACATTTATCCAGCCGCTTTTGCGCGTaaa > 1:3088638/1‑65 (MQ=255) acacTGCTTACAGGCTGGGTTCTCTTCGCCGAACACACATTTATCCCTCCGCTTTTTCTCGTtaa > 1:593389/1‑65 (MQ=255) acacTGCTTACAGGCTGGGTTCTCTTCGCCGAAAACACATTTATCCAGCCGCTTTTGCGCGTaaa > 1:440037/1‑65 (MQ=255) acacTGCTTACAGGCTGGGTTCTCTTCGCCCAAAACACATTTATTCAGCCGCTTTTGCGCGGaaa > 1:2263902/1‑65 (MQ=255) acTGCTTACAGGCTGGTTTCTCTTCGCCGAACACACATTTATCCAGCCGCTTTTGCGCGTaaaca > 1:2206259/1‑65 (MQ=255) acTGCTTACAGGCTGGTTTCTCTTCCCCGAACACACATTTATCCAGCCGCCTTTGCGCCTaaaca > 1:2242685/1‑65 (MQ=255) acTGCTTACAGGCTGGTTTCCCTTCGCCGAACACACATTTATCCAGCCGCTTTTGCCCGTcaaca > 1:41282/1‑65 (MQ=255) acTGCTTACAGGCTGGGTTCTCTTTGCCGAACACACCTTTATCCAGCCGCTTTTTCGCGTaaaca > 1:2680997/1‑65 (MQ=255) acTGCTTACAGGCTGGGTTCTCTTGTCCGAAACCCCATTTATCCCAGCGCTTTTTCCCGTaaaca > 1:184163/1‑65 (MQ=255) acTGCTTACAGGCTGGGTTCTCTTCGCCGAACACACATTTATCCGGCCGCTTTTGCGCGTaaaca > 1:1193530/1‑65 (MQ=255) acTGCTTACAGGCTGGGTTCTCTTCGCCGAACACACATTTATCCAGCCGCTTTTGCGCGTaaaca > 1:1454680/1‑65 (MQ=255) cTGCTTACAGGCTGGTTTCTCTTCGCCGAACACACATTTATCCAGCCGCTTTTGCGCGTaaacaa > 1:3306684/1‑65 (MQ=255) gCTTACAGGCTGGTTTCTCTTCGCCGAACCCACATTTATCCAGCCGCTTTTGCGCGTaaacaaac > 1:1009162/1‑65 (MQ=255) cAGGCTGGTTTCTCTTCGCCGAACACACATTTATCCAGCCGCTTTTGCGCGTAAACAAACAACGc > 1:460179/1‑65 (MQ=255) cAGGCTGGTTTCTCTTCGCCGAACACACATTTATCCAGCCGCTTTTGCGCGTAAACAAACAACGc > 1:108914/1‑65 (MQ=255) aGGCTGGTTTCTCTTCGCCGAACCCACATTTATCCAGCCGCTTTTGCGCGTAAACAAACAACGcc < 1:3004906/65‑1 (MQ=255) aGGCTGGTTTCTCTTCGCCGAACACACATTTATCCAGCCGCTTTTGCGCGTAAACAAACAACGcc < 1:2119767/65‑1 (MQ=255) gCTGGTTTCTCTTCGCCGAACACACATTTATCCAGCCGCTTTTGCGCGTAAACAAACAACGCCTc < 1:569023/65‑1 (MQ=255) gCTGGTTTCTCTTCGCCGAACACACATTTATCCAGCCGCTTTTGCGCGTAAACAAACAACGCCTc < 1:647425/65‑1 (MQ=255) | TCTGTTTCATCTCTTCACGCTTTGCAGGCTGGTAACAGTGCACCGGACACTGCTTACAGGCTGGTTTCTCTTCGCCGAACACACATTTATCCAGCCGCTTTTGCGCGTAAACAAACAACGCCTC > NC_000913/2856581‑2856704 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |