| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,296,060 | 0 | C | T | 31.8% | 80.3 / 52.0 | 63 | intergenic (+266/+376) | gltP/yjcO | glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO |
| Reads supporting (aligned to +/- strand): ref base C (28/15); new base T (10/10); total (38/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.81e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
AATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGAT > NC_000913/4295997‑4296120 | aaTAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACg < 1:2865099/65‑1 (MQ=35) aTAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGc > 1:1053109/1‑65 (MQ=35) aTAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGc > 1:2079207/1‑65 (MQ=35) aGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCt > 1:942349/1‑65 (MQ=25) aGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCt > 1:1149703/1‑65 (MQ=25) aGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCt > 1:522896/1‑65 (MQ=25) aGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCt > 1:1652022/1‑65 (MQ=25) aGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCt > 1:3113343/1‑65 (MQ=35) aGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCt > 1:1347566/1‑65 (MQ=35) aGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCt > 1:2015307/1‑65 (MQ=35) aGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTCACGCCGCACCCGACATCAACCCCt > 1:2091582/1‑65 (MQ=11) ccTGATTTAGCGTGATTTTGTAGGTCGGATACGGCGTTTACGCCGCCGCCGACGTCAACGCCtta > 1:3272353/1‑63 (MQ=11) ccTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGa > 1:986518/1‑65 (MQ=25) ccTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGa > 1:3067810/1‑65 (MQ=25) ccTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGa > 1:2074543/1‑65 (MQ=25) ccTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGa > 1:2282394/1‑65 (MQ=35) ccTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGa > 1:2273086/1‑65 (MQ=35) ccTGATTTAGCGTGATTTTGTACGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGa > 1:2010353/1‑65 (MQ=255) cTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGAt < 1:2839281/65‑1 (MQ=35) tttAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGa < 1:2992197/65‑1 (MQ=35) tttAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGa > 1:2747094/1‑65 (MQ=35) tttAGCGTGATTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGACATCAAGGCCTGTTACGa > 1:3427209/1‑65 (MQ=11) ttAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGAc > 1:1600846/1‑65 (MQ=35) ttAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGAc > 1:285152/1‑65 (MQ=35) ttAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGAc > 1:2092727/1‑65 (MQ=35) tAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACg > 1:585166/1‑65 (MQ=35) gCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCt < 1:3125339/65‑1 (MQ=35) cGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCtt > 1:2532350/1‑65 (MQ=35) tttGTAGGTCGGATACGGCGTTTACGCCGCCTACTACATCAATGCCTGATGCGACGCTTGCcgcg < 1:1435896/65‑1 (MQ=14) tttGTAGGTCGGATAAGGCGTTTCCGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCcgcg < 1:89606/65‑1 (MQ=255) tttGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCcgcg < 1:1682406/65‑1 (MQ=255) aGGTCGGATAAGGCGTTTACGCTGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTa < 1:1545247/65‑1 (MQ=14) aGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTa > 1:77436/1‑65 (MQ=255) aGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTa < 1:3235083/65‑1 (MQ=255) aGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTa > 1:1877548/1‑65 (MQ=255) gTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATc > 1:2720107/1‑65 (MQ=255) ggATAAGGCTTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAgg < 1:2018000/65‑1 (MQ=255) ggATAAGGCGTTTCCGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAgg < 1:1706062/65‑1 (MQ=255) ggATAAGGCGTTTACGCCGGATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAgg < 1:548456/65‑1 (MQ=255) ggATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAgg < 1:1291270/65‑1 (MQ=255) ggATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAgg < 1:3305903/65‑1 (MQ=255) ggATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAgg < 1:211931/65‑1 (MQ=255) ggATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAgg < 1:2286435/65‑1 (MQ=255) aaGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTa > 1:1920274/1‑65 (MQ=255) aGGCGTTTCCGCCGCATCCGACATCAATGCCTGATGCGCCGCTTGCCGCGTCTTATCAGGCCTat < 1:3418472/65‑2 (MQ=11) aGGCGTTTCCGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTAc < 1:1081520/65‑1 (MQ=255) aGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTat < 1:1558748/65‑2 (MQ=255) aGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTat < 1:1259033/65‑2 (MQ=255) aGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTat < 1:3395654/65‑2 (MQ=255) aGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTat < 1:1986105/65‑2 (MQ=255) aGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTAc < 1:3336785/65‑1 (MQ=255) aGGCCTTTGCGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTAc < 1:1001715/65‑1 (MQ=255) ggCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACg > 1:1590488/1‑65 (MQ=255) gCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTatct < 1:2683891/65‑4 (MQ=255) gCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTatct > 1:3329236/1‑62 (MQ=255) gCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGc > 1:1901301/1‑65 (MQ=255) cttttACCCCCCATCCGACATCAACGCCTGATGCGCCGCTTGCCGCGTCTTATCAGGCCTACGcc < 1:1515859/63‑1 (MQ=14) cGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTAcctc > 1:2102064/1‑62 (MQ=255) cGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGcc > 1:3052309/1‑65 (MQ=255) cGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGcc > 1:1279558/1‑65 (MQ=255) cGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGGCTTATCAAGCCTAAGcc > 1:2881915/1‑65 (MQ=14) cGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCCTGCCGCGTTTTATCAGGCCTACGcc > 1:3289996/1‑65 (MQ=255) tttCCGCCGCACCCGCCATCAACGCCTGATGCGACGCTTGCCGCTTCTTATCAGGCCTACGCCAg < 1:692243/65‑1 (MQ=14) tACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGAc > 1:2356198/1‑65 (MQ=255) aCGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACa > 1:262156/1‑65 (MQ=255) aCGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACa > 1:1492869/1‑65 (MQ=255) gccgcATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAgc > 1:1737266/1‑65 (MQ=40) gccgcATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAgc < 1:1800776/65‑1 (MQ=40) gccgcATCCGACACCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAgc < 1:1182313/65‑1 (MQ=255) gccgcACCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAgc < 1:3085388/65‑1 (MQ=255) cgcATCCGCCATCAACGCCTGATGCGACGCTGGCCGCGTCTTATCAGGCCTACGCCAGACAgcgc < 1:2331444/65‑1 (MQ=255) cgcATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAgcgc < 1:2249885/65‑1 (MQ=40) cgcATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAgcgc < 1:2082608/65‑1 (MQ=40) cccATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAgcgc < 1:756364/63‑1 (MQ=255) gcATCCCACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCa < 1:295115/65‑1 (MQ=255) ccaCATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGc < 1:3340778/63‑1 (MQ=255) cGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGc > 1:820690/1‑65 (MQ=40) aTCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGa < 1:388953/65‑1 (MQ=40) tCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCACTAGCCTgct > 1:78201/1‑63 (MQ=255) tCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGAt > 1:3456210/1‑65 (MQ=39) tCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGAt < 1:3410634/65‑1 (MQ=39) tCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGAt > 1:2223300/1‑65 (MQ=39) tCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGAt < 1:3263641/65‑1 (MQ=39) | AATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGAT > NC_000913/4295997‑4296120 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |