Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,390,754	G→T	A252A (GCC→GCA)	rsgA ←	ribosome small subunit‑dependent GTPase A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,390,754	0	G	T	100.0%	103.6 / NA	29	A252A (GCC→GCA)	rsgA	ribosome small subunit‑dependent GTPase A
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (22/7);  total (22/7)

CGCACTCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGTGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGATCTC  >  NC_000913/4390698‑4390813                                                         |                                                            cgcgCTCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGTCgt                                                     <  1:617753/65‑1 (MQ=255) cGCACTCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGTCgt                                                     <  1:3202693/65‑1 (MQ=255)    aCTCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGTCgcggt                                                  >  1:2733606/1‑65 (MQ=255)      tCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGgtgt                                                <  1:1881573/65‑1 (MQ=255)      tCCGGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGgtgt                                                <  1:2567341/65‑1 (MQ=255)      tCCGGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGgtgt                                                <  1:2024623/65‑1 (MQ=255)            ggAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGGTGTGCTGaa                                          >  1:375795/1‑64 (MQ=255)              aGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGGTGTGCTGACCg                                        >  1:2337530/1‑65 (MQ=255)              aGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGGTGTGCTGACCg                                        >  1:2933208/1‑65 (MQ=255)              aGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGGTGTGCTGACCg                                        >  1:642725/1‑65 (MQ=255)              aGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGGTGTGCTGACCg                                        >  1:164856/1‑65 (MQ=255)              aGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGGTGTGCTGACCg                                        >  1:612183/1‑65 (MQ=255)              aGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGGTGTGCTGACCg                                        >  1:2329550/1‑65 (MQ=255)              aGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGGTGTGCTGACCg                                        >  1:1989306/1‑65 (MQ=255)              aGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGTCGGGGTGTGCTGActg                                        >  1:550910/1‑63 (MQ=255)              aGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGCCGTGGTGTGCTGACCg                                        >  1:2390703/1‑65 (MQ=255)                    tcacatcacCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGGTGTGCTGACCGAGcccc                                  >  1:1797241/1‑65 (MQ=255)                      acatcacCGCCGTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGGTGGGCTGACCGAGCCCCGa                                >  1:1029859/1‑65 (MQ=255)                              gccgTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGGTGTGCTGACCGAGCCCCGAGTTGTCag                        >  1:2759685/1‑65 (MQ=255)                              gccgTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGGTGTGCTGACCGAGCCCCGAGTTGTCag                        >  1:3172920/1‑65 (MQ=255)                              gccgTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGGTGTGCTGACCGAGCCCCGAGCTGTCag                        >  1:2402125/1‑65 (MQ=255)                               ccgTGCGGGAAGTGATACAGCCGAGCTGCGGTCGTGGTGTGCTGCCCGAGCCCCGAGTTGTCaga                       <  1:1590260/65‑1 (MQ=255)                                 tggCGGGAAGGGTTGCAGCCGAGCTGCGGTCGTTGTGTGCTGGCCGAGCCCCGAGTTGTCAgaga                     <  1:3457745/63‑1 (MQ=255)                                   gggggAAGTGGTCCAGCCGAGCTGCGGTCGTGGTGTGCTGACCGAGCCCCGAGTTGTCAGAGata                   <  1:2059374/63‑1 (MQ=255)                                         aGTGATACAGCCGAGCTGCGGTCGTGGTGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTg             >  1:2229649/1‑65 (MQ=255)                                               aCAGCCGAGCTGCGGTCGTGGTGTGCTGTCCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAgg       <  1:780523/65‑1 (MQ=255)                                               aCAGCCGAGCTGCGGTCGTGGTGTGCTGACCGAGCCCCGAGTTGTCAGAGATCTCGTTGGTCAgg       >  1:2336989/1‑65 (MQ=255)                                               aCAGCCGAGCTGCGGTCGTGGTGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAgg       <  1:1048995/65‑1 (MQ=255)                                               aCAGCCGAGCTGCGGTCGTGGTGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAgg       >  1:3046745/1‑65 (MQ=255)                                               aCAGCCGAGCTGCGGTCGTGGTGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAgg       >  1:2693117/1‑65 (MQ=255)                                               aCAGCCGAGCTGCGGTCGTGGTGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAgg       <  1:1486037/65‑1 (MQ=255)                                               aCAGCCGAGCTGCGGTCGTGGTGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAgg       <  1:1197832/65‑1 (MQ=255)                                                 aGCCGAGCTGCGGTCGTGGTGTGCTGACCGAGCCCCGCGTTGTCAGAGATATCGTTGGTCAGGac     >  1:1674687/1‑64 (MQ=255)                                                 aGCCGAGCTGCGGTCGTGGTGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGAt     >  1:1927427/1‑65 (MQ=255)                                                  gCCGAGCTGCGGTCGTGGTGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGAtc    >  1:20480/1‑65 (MQ=255)                                                  gCCGAGCTGCGGTCGTGGTGTGCTGACCGAGCCCCGAGTTGGCAGAGATATCGTTGGTCAGGAtc    >  1:1497722/1‑65 (MQ=255)                                                   ccGAGCTGCGGTCGTGGTGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGAtct   >  1:948158/1‑65 (MQ=255)                                                    cGAGCTGCGGTCGTGGTGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGAtctc  >  1:2636260/1‑65 (MQ=255)                                                    cGAGCTGCGGTCGTGGTGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGAtctc  <  1:799470/65‑1 (MQ=255)                                                         |                                                            CGCACTCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAGTGATACAGCCGAGCGGCGGTCGTGGTGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGATCTC  >  NC_000913/4390698‑4390813

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑