Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	50,102	A→C	I94L (ATT→CTT)	folA →	dihydrofolate reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	50,102	0	A	C	100.0%	116.3 / NA	35	I94L (ATT→CTT)	folA	dihydrofolate reductase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (24/11);  total (24/11)

CGTGGGTGAAGTCGGTGGATGAAGCCATCGCGGCGTGTGGTGACGTACCAGAAATCATGGTGATTGGCGGCGGTCGCGTTTATGAACAGTTCTTGCCAAAAGCGCAAAAACTGTATCTGACGC  >  NC_000913/50040‑50162                                                               |                                                             cGTGGGTGAAGTCGGTGGATGAAGCCATCGCGGCGTGTGGTGACGTACCAGAAATCATGGTGCtt                                                            >  1:3328228/1‑65 (MQ=255) cGTGGGTGAAGTCGGTGGATGAAGCCATCGCGGCGTGTGGTGACGTACCAGAAATCATGGTGCtt                                                            >  1:3053308/1‑65 (MQ=255) cGTGGGTGAAGTCGGTGGATGAAGCCATCGCGGCGTGTGGTGACGTACCAGAAATCATGGTGCtt                                                            >  1:3355649/1‑65 (MQ=255) cGTGGGTGAAGTCGGTGGATGAAGCCATCGCGGCGTGTGGTGACGTACCAGAAATCATGGTGCtt                                                            >  1:2065049/1‑65 (MQ=255) cGTGGGTGAAGTCGGGGGATGAAGCCATCGCGGCGTGTGGTGACGTACCAGAAATCATAGTGCtt                                                            >  1:2164250/1‑65 (MQ=255)   tGGGTGAAGTCGGTGGATGAAGCCATCGCGGCGTGTGGTGACGTACCAGAAATCATGGTGCTTgg                                                          >  1:3125216/1‑65 (MQ=255)   tGGGTGAAGTCGGTGGATGAAGCCATCGCGGCGTGTGGTGACGTACCAGAAATCATGGTGCTTgg                                                          >  1:3342911/1‑65 (MQ=255)        gAAGTCGGTGGATGAAGCCATCGCGGCGTGTGGTGACGTACCAGAAATCATGGTGCTTGgcggcg                                                     >  1:3177677/1‑65 (MQ=255)            tCGGTGGATGAAGCCATCGCGGCGTGTGGTGACGTACCAGAAATCATGGTGCTTGGcggcggctg                                                 >  1:2123858/1‑62 (MQ=255)                  gATGAAGCCATCGCGGCGTGTGGTGACGTACCAGAAATCATGGTGCTTGGCGGCGGCCGCGTTTa                                           >  1:2088465/1‑65 (MQ=255)                   aTGAAGCCATCGCGGCGTGTGGTGACGTACCAGAAATCATGGTGCTTGGCGGCGGTCGCGTTTAt                                          >  1:3069182/1‑65 (MQ=255)                     gAAGCCATCGCGGCGTGTGGTGACGTACCAGAAATCATGGTGCTTGGCGGCGGTCGCGTTTATGa                                        <  1:1514394/65‑1 (MQ=255)                     gAAGCCATCGCGGCGTGTGGTGACGTACCAGAAATCATGGTGCTTGGCGGCGGTCGCGTTTATGa                                        <  1:816867/65‑1 (MQ=255)                        gCCATCGCGGCGCGTGGTGACGTACCAGAAATCATGGTGCTTGGCGGCGGTCGCGTTTATGaata                                     >  1:2629220/1‑63 (MQ=38)                         ccATCGCGGCGTGTGGTGACGTACCAGAAATCATGGTGCTTGGCGGCGGTCGCGTTTATGAACAg                                    >  1:1623743/1‑65 (MQ=255)                          cATCGCGGCGTGTGGTGACGTACCAGAAATCATGGTGCTTGGCGGCGGTCGCGTTTATGAACAGt                                   <  1:1752906/65‑1 (MQ=255)                             cgcgGCGTGTGGTGACGTACCAGAAATCATGGTGCTTGGCGGCGGTCGCGTTTATGAACAGTTCt                                <  1:3052655/65‑1 (MQ=255)                                  cgTGTGGTGACGTACCAGAAATCATGGTGCTTGGCGGCGGTCGCGTTTATGAACAGTTCTTGCCa                           >  1:2851583/1‑65 (MQ=255)                                          gACGTACCAGAAATCATGGTGCTTGGCGGCGGTCGCGTTTATGAACAGTTCTTGCCAAAAGCGCa                   >  1:1464903/1‑65 (MQ=255)                                          gACGTACCAGAAATCATGGTGCTTGGCGGCGGTCGCGTTTATGAACAGTTCTTGCCAAAAGCGCa                   >  1:3043795/1‑65 (MQ=255)                                          gACGTACCAGAAATCATGGTGCTTGGCGGCGGTCGCGTTTATGAACAGTTCTTGCCAAAAGCGCa                   <  1:1457513/65‑1 (MQ=255)                                           aCGTACCAGAAATCATGGTGCTTGGCGGCGGTCGCGTTTATGAACAGTTCTTGCCAAAAGCGCaa                  >  1:725323/1‑65 (MQ=255)                                            cGTACCAGAAATCATGGTGCTTGGCGGCGGTCGCGTTTATGAACAGTTCTTGCCAAAAGCGCaaa                 >  1:1517895/1‑65 (MQ=255)                                            cGTACCAGAAATCATGGTGCTTGGCGGCGGTCGCGTTTATGAACAGTTCTTGCCAAAAGCGCaaa                 >  1:591318/1‑65 (MQ=255)                                            cGTACCAGAAATCATGGTGCTTGGCGGCGGTCGCGTTTATGAACAGTTCTTGCCAAAAGCGCaaa                 >  1:2139063/1‑65 (MQ=255)                                            cGTACCAGAAATCATGGTGCTTGGCGGCGGTCGCGTTTATGAACAGTTCTTGCCAAAAGCGCaaa                 >  1:1482336/1‑65 (MQ=255)                                            cGTACCAGAAATCATGGTGCTTGGCGGCGGTCGCGTTTATGAAAAGTTCTTGCCAAAAGCGCaaa                 >  1:1970303/1‑65 (MQ=39)                                              tACCAGAAATCATGGTGCTTGGCGGCGGTCGCGTTTATGAACAGTTCTTGCCAAAAGCGCaaaaa               <  1:2748552/65‑1 (MQ=255)                                              tACCAGAAATCATGGTGCTTGGCGGCGGTCGCGTTTATGAACAGTTCTTGCCAAAAGCGCaaaaa               <  1:51811/65‑1 (MQ=255)                                              tACCAGAAATCATGGTGCTTGGCGGCGGTCGCGTTTATGAACAGTTCTTGCCAAAAGCGCaaaaa               <  1:1058621/65‑1 (MQ=255)                                                ccAGAAATCATGGTGCTTGGCGGCGGTCGCGTTTATGAACAGTTCTTGCCAAAAGCGCAAAAACt             <  1:3298182/65‑1 (MQ=255)                                                 cAGAAATCATGGTGCTTGGCGGCGGTCGCGTTTATGAACAGTTCTTGCCAAAAGCGCAAAAACTg            >  1:2749597/1‑65 (MQ=255)                                                  aGAAATCATGGTGCTTGGCGGCGGTCGCGTTTATGAACAGTTCTTGCCAAAAGCGCAAAAACTGt           <  1:934638/65‑1 (MQ=255)                                                     aaTCATGGTGCTTGGCGGCGGTCGCGTTTATGAACAGTTCTTGCCAAAAGCGCAAAAACTGTATc        >  1:3298629/1‑65 (MQ=255)                                                       tCATGGTGCTTGGCGGCGGTCGCGTTTATGAACAGTTCTTGCCAAAAGCGCAAAAACTGTATCTg      >  1:52867/1‑65 (MQ=255)                                                        cATGGTGCTTGGCGGCGGTCGCGTTTATGAACAGTTCTTGCCAAAAGCGCAAAAACTGTATCTGa     <  1:2764148/65‑1 (MQ=255)                                                           ggTGCTTGGCGGCGGTCGCGTTTATGAACAGTTCTTGCCAAAAGCGCAAAAACTGTATCTGACGc  >  1:34996/1‑65 (MQ=255)                                                           ggTGCTTGGCGGCGGTCGCGTTTATGAACAGTTCTTGCCAAAAGCGCAAAAACTGTATCTGACGc  >  1:1147460/1‑65 (MQ=255)                                                           ggTGCTTGGCGGCGGTCGCGTTCGTGAACAGTTCTTGCCAAAAGCGCAAAAACTGTATCTGACgt  >  1:387920/1‑64 (MQ=255)                                                               |                                                             CGTGGGTGAAGTCGGTGGATGAAGCCATCGCGGCGTGTGGTGACGTACCAGAAATCATGGTGATTGGCGGCGGTCGCGTTTATGAACAGTTCTTGCCAAAAGCGCAAAAACTGTATCTGACGC  >  NC_000913/50040‑50162

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑