Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,905,761	G→A	G25D (GGT→GAT)	mntP →	Mn(2(+)) exporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,905,761	0	G	A	100.0%	107.1 / NA	29	G25D (GGT→GAT)	mntP	Mn(2(+)) exporter
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (19/10);  total (19/10)

GCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGGTGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGC  >  NC_000913/1905700‑1905821                                                              |                                                             gCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGATGc                                                           <  1:895184/65‑1 (MQ=255) gCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGATGc                                                           >  1:7976/1‑65 (MQ=255)  cTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGATGcc                                                          <  1:2408694/65‑1 (MQ=255)    aCTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGATGCCAc                                                        >  1:2584515/1‑65 (MQ=255)    aCTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGATGCCAc                                                        <  1:1679097/65‑1 (MQ=255)    aCTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGATGCCAc                                                        >  1:1857620/1‑65 (MQ=255)    aCTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGATGCCAc                                                        <  1:332732/65‑1 (MQ=255)    aCTGTTCTTCTTGCGTTTGGCATGTCGATGGATGCATTTGCTGCTTTAATCGGTTAAGATGCCAc                                                        >  1:1122633/1‑65 (MQ=255)     cTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGATGCCAcc                                                       <  1:1138118/65‑1 (MQ=255)      tGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATTAATCGGTAAAGATGCCAccc                                                      >  1:1989365/1‑65 (MQ=255)        ttcttcTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGATGCCACCCTc                                                    >  1:2457821/1‑65 (MQ=255)         tcttctTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAGTCGGTAAAGATGCCACCCTcc                                                   >  1:2523694/1‑65 (MQ=255)         tcttctTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGATGCCACCCttc                                                   >  1:2830525/1‑63 (MQ=255)         tcttctTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGATGCCACCCTcc                                                   <  1:2273598/65‑1 (MQ=255)         tcttctTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGATGCCACCCTcc                                                   >  1:224753/1‑65 (MQ=255)         tcttctTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGATGCCACCCTcc                                                   >  1:52989/1‑65 (MQ=255)         tcttctTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGATGCCACCCTcc                                                   >  1:1622577/1‑65 (MQ=255)         tcttctTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGATGCCACCCTcc                                                   <  1:3206246/65‑1 (MQ=255)                       gTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGATGCCACCCTCCATAAACCGAAAttt                                     >  1:2632323/1‑65 (MQ=255)                        tATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGATGCCACCCTCCATAAACCGAAAtttt                                    >  1:261832/1‑65 (MQ=255)                          tGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCt                                  >  1:241774/1‑65 (MQ=255)                               atggatgCATTTGCTGCATCAATCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGc                             <  1:1878947/65‑1 (MQ=255)                                  gatgCATTTGCTGCATCAATCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCAtt                          >  1:3428966/1‑65 (MQ=255)                                      gaTTTCCTGCATCAATCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGa                      <  1:3242408/64‑1 (MQ=255)                                        tttGCTGCATCAATCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAAc                    >  1:2623897/1‑65 (MQ=255)                                           gctgcATCAATCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCgg                 >  1:1316338/1‑65 (MQ=255)                                            ctgcATCAATCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGc                <  1:827662/65‑1 (MQ=255)                                             tgcATCAATCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGcc               >  1:3177753/1‑65 (MQ=255)                                               cATCAATCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGCCtt             >  1:2725345/1‑65 (MQ=255)                                                aTCAATCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGCCTTa            <  1:750993/65‑1 (MQ=255)                                                   aaTCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGCCTTAttt         <  1:2553247/65‑1 (MQ=255)                                                        gTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGCCTTATTTTTGGt    >  1:935682/1‑65 (MQ=255)                                                          aaaGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGc  >  1:3412897/1‑65 (MQ=255)                                                              |                                                             GCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGGTGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGC  >  NC_000913/1905700‑1905821

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑