| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 987,014 | 0 | A | C | 27.3% | 41.6 / 12.1 | 22 | intergenic (‑32/+571) | ompF/asnS | outer membrane porin F/asparagine‑‑tRNA ligase |
| Reads supporting (aligned to +/- strand): ref base A (16/0); new base C (0/6); total (16/6) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.34e-05 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GACGATCACTGCCAGAATATTGCGCTTCATCATTATTTATTACCCTCATGGTTTTTTTTA‑TG‑ACACCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAG > NC_000913/986950‑987072 | gACGATCACTGCCAGAATATTGCGCTTCATCATTATTTATTACCCTCATGGTTTTTTTTA‑TG‑aca > 1:2126773/1‑65 (MQ=255)gACGATCACTGCCAGAATATTGCGCTTCATCATTATTTATTACCCTCATGGTTTTTTTTA‑TG‑aca > 1:1613838/1‑65 (MQ=255)gACGATCACTGCCAGAATATTGCGCTTCATCATTATTTATTACCCTCATGGTTTTTTTTA‑TG‑aca > 1:1594882/1‑65 (MQ=255)gACGATCACTGCCAGAATATTGCGCTTCATCATTATTTATTACCCTCATGGTTTTTTTTA‑TG‑aca > 1:1351013/1‑65 (MQ=255) tCACTGCCAGAATATTGCGCTTCATCATTATTTATTACCCTCATGGTTTTTTTTA‑TG‑ACACCTGc > 1:1876699/1‑65 (MQ=255) aCTGCCAGAATATTGCGCTTCATCATTATTTATTACCCTCATGGTTTTTTTTA‑TG‑ACACCTGCCa > 1:3035577/1‑65 (MQ=255) aCTGCCAGAATATTGCGCTTCATCATTATTTATTACCCTCATGGTTTTTTTTA‑TG‑ACACCTGCCa > 1:1726143/1‑65 (MQ=255) tGCCAGAATATTGCGCTTCATCATTATTTATTACCCTCATGGTTTTTTTTA‑TG‑ACACCTGCCACt > 1:1203824/1‑65 (MQ=255) ccAGAATATTGCGCTTCATCATTATTTATTACCCTCATGGTTTTTTTTA‑TG‑ACACCTGCCACTGc > 1:123666/1‑65 (MQ=255) gAATATTGCGCTTTTTAATTTTTTATCGCCCTCATGTTTTTTTTTA‑TC‑ACCCCTGCCACTGCCGt < 1:2210453/65‑1 (MQ=255) aaTATTGCGCTTCATCATTATTTATTACCCTCATGGTTTTTTTTA‑TG‑ACACCTGCCACTGCCGTc > 1:1117301/1‑65 (MQ=255) atTGCGCTTCATCATTATTTATTACCCTCATGGTTTTTTTTA‑TG‑ACACCTGCCACTGCCGTCAAt > 1:2957806/1‑65 (MQ=255) tGCGCTTCATCATTTTTTATTCCCCTCATGTTTTTTTTTA‑TC‑ACCCCTGCCACTGCCGTCAATaa < 1:121897/65‑1 (MQ=255) tGCGCTTCATCATTATTTATTACCCTCATGGTTTTTTTTA‑TG‑ACACCTGCCACTGCCGTCAATaa > 1:2991219/1‑65 (MQ=255) ctcTTCATCATTTTTTATTCCCCGCAGGTTTTTTTTTA‑TG‑CCCCCTGCCACTGCCGTCAATAAGt < 1:1631324/63‑1 (MQ=255) tttattACCCTCATGGTTTTTTTTA‑TG‑ACACCTGCCACTGCCGTCAATAAGTTCTGTCAATaaaa > 1:2503121/1‑65 (MQ=255) ttattaCCCTCATGGTTTTTTTTA‑TG‑ACACCTGCCACTGCCGTCAATAAGTTCTGTCAATaaaaa > 1:2635941/1‑65 (MQ=255) ccTCATGGTTTTTTTTA‑TG‑ACACCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACgg > 1:1675400/1‑65 (MQ=255) ccTCATGGTTTTTTTTA‑TG‑ACACCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACgg > 1:261007/1‑65 (MQ=255) ccTCATGGTTTTTTTTA‑TG‑ACACCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACgg > 1:2313102/1‑65 (MQ=255) ttgTTTTTTTTA‑AGCCCCCCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACt < 1:1081751/63‑1 (MQ=255) ttttttttttT‑GG‑CCCCCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTAt < 1:289736/63‑1 (MQ=255) ttttttttttA‑CG‑CCCCCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTAt < 1:616455/63‑1 (MQ=255) gtttttttttG‑TG‑CCCCCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTAt < 1:161105/63‑1 (MQ=255) gtttttttttA‑CG‑CCCCCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTAt < 1:2046573/63‑1 (MQ=255) ggTTTTTTTTA‑TG‑ACACCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTAt > 1:1344271/1‑65 (MQ=255) ggTTTTTTTTA‑TC‑CCCCCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTAt < 1:1789694/65‑1 (MQ=255) gTTTTTTTTACTC‑GCCCCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTAt < 1:1713060/65‑1 (MQ=255) tttttttA‑TG‑ACCCCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATtga < 1:1908564/65‑1 (MQ=255) ttttA‑TG‑ACACCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATtgatga > 1:222545/1‑65 (MQ=255) ttttA‑TG‑ACACCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATtgatga > 1:103502/1‑65 (MQ=255) tA‑TG‑ACCCCTGCCCCTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGagag < 1:2500267/65‑1 (MQ=255) | GACGATCACTGCCAGAATATTGCGCTTCATCATTATTTATTACCCTCATGGTTTTTTTTA‑TG‑ACACCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAG > NC_000913/986950‑987072 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |