| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,374,036 | 0 | T | G | 27.8% | 28.1 / 13.1 | 18 | L272R (CTC→CGC) | ycjO | putative ABC transporter membrane subunit YcjO |
| Reads supporting (aligned to +/- strand): ref base T (4/9); new base G (0/5); total (4/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.78e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.15e-01 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TATCTCTACAAAACCGCCTTTGCCTTTAATGATTTAGGAAAAGCGGCGGCGATCTCGGTGGTGCTCTTCATCATTATTTTCGCTGTCATTCTGCTGACCAGGAAACGGGTGAACCTCAATGGCAACAAA > NC_000913/1373972‑1374100 | tatCTCTACAAAACCGCCTTTGCCTTTAATGATTTAGGAAAAGCGGCGGCGATCTCGGTGGTGct < 1:1248927/65‑1 (MQ=255) tctcTACAAAACCGCCTTTGCCTTTAATGATTTAGGAAAAGCGGCGGCGATCTCGGTGGTGctct > 1:1601188/1‑65 (MQ=255) tctcTACAAAACCGCCTTTGCCTTTAATGATTTAGGAAAAGCGGCGGCGATCTCGGTGGGGgtct > 1:2810777/1‑65 (MQ=255) tctcTACAAAACCGCCTTTGCCTTTAATGATTTAGGAAAAGCGGCGGCGATCTCGGTGGGGctct > 1:1380827/1‑65 (MQ=255) tctcTACAAAACCGCCTTTGCCTTTAATGATTTAGGAAAAGCGGCGGCGAGCTCGGTGGTGctct > 1:2537332/1‑65 (MQ=255) tctACAAAACCGCCTTTGCCTTTAATGATTTAGGAAAAGCGGCGGCGGTCTCGGGGGGGCTTTtc > 1:1703201/1‑65 (MQ=255) tctACAAAACCGCCTTTGCCTTTAATGATTTAGGAAAAGCGGCGGCGATCTCGGTGGTGCTCTtc < 1:2800701/65‑1 (MQ=255) ctACCAAACCGCCTTTGCCTTTAATGATTTAGGAAAAGCGGCGGCGATCTCGGTGGTGCTCTtca < 1:1317849/65‑1 (MQ=255) aCCGCCTTTGCCTTTAATGATTTAGGAAAAGCGGCGGCGGTCTCGGTGGTGCTCTTCATCattat > 1:1172573/1‑65 (MQ=255) aCCGCCTTTGCCTTTAATGATTTAGGAAAAGCGGCGGCGGGCTCGGTGGGGCTCTTCATCagtat > 1:3028620/1‑65 (MQ=255) aCCGCCTTTGCCTTTAATGATTTAGGAAAAGCGGCGGCGGAATCGGTGGCTTTTTTCATCatttt > 1:658114/1‑63 (MQ=255) aCCGCCTTTGCCTTTAATGATTTAGGAAAAGCGGCGGCGATCTCGGTGGGGCTCTTCATCattat > 1:756964/1‑65 (MQ=255) ccGCCTTTGCCTTTAATGATTTAGGAAAAGCGGCGGCGATCTCGGTGGTGCTCTTCATCattatt < 1:551574/65‑1 (MQ=255) ctttgcctttAATGATTTAGGAAAAGCGGCGGCGATCTCGGTGGTGCTCTTCATCATTATTTTCg > 1:1875703/1‑65 (MQ=255) gcctttAATGATTTGGGAGAAGGGGTGTAGATCTCGGTGGT‑CGCCGTATCATTATTTTCGCTGTc < 1:783282/65‑1 (MQ=255) gcctttAATGATTTAGGAAAAGCGGCGGCGATCTCGGTGGTGCTCTTCATCATTATTTTCGCTGt < 1:1572020/65‑1 (MQ=255) tAGGAAAAGCGGCGGCGATCTCGGTGGTGCTCTTCATCATTATTTTCGCTGTCATTCTGCTGAcc < 1:329671/65‑1 (MQ=255) ggcgcgtcgATCTCGGTGGT‑CGCCGTATCATTATTTTCGCTGTCATTCTGCTGACCAGGAAACgg < 1:2376886/62‑1 (MQ=255) gcggcggcgATCTCGGTGGTGCTCTTCATCATTATTTTCGCTGTCATTCTGCTGACCAGGAAACg < 1:963523/65‑1 (MQ=255) ggcggcgATCTCGGTGGTGCTCTTCATCATTATTTTCGCTGTCATTCTGCTGACCAGGAAACggg < 1:1567794/65‑1 (MQ=255) tagATCTCGGTGGT‑CGCCGTATCATTATTTTCGCTGTCATTCTGCTGACCAGGAAACGGGTGAAc < 1:2428665/63‑1 (MQ=255) cgATCTCGGTGGT‑CGCCGTATCATTATTTTCGCTGTCATTCTGCTGACCAGGAAACGGGTGAAcc < 1:2703502/65‑1 (MQ=255) agATCTCGGTGGT‑CGCCGTATCATTATTTTCGCTGTCATTCTGCTGACCAGGAAACGGGTGAAcc < 1:1765329/64‑1 (MQ=255) gATCTCGGTGGTGCTCTTCATCATTATTTTCGCTGTCATTCTGCTGACCAGGAAACGGGTGAAcc < 1:2741376/65‑1 (MQ=255) gtggtgCTCTTCATCATTATTTTCGCTGTCATTCTGCTGACCAGGAAACGGGTGAACCTCAATgg > 1:22714/1‑65 (MQ=255) gtggtgCTCTTCATCATTATTTTCGCTGTCATTCTGCTGACCAGGAAACGGGTGAACCTCAATgg > 1:360425/1‑65 (MQ=255) gtggtgCTCTTCATCATTATTTTCGCTGTCATTCTGCTGACCAGGAAACGGGTGAACCTCAATgg > 1:1811831/1‑65 (MQ=255) ggtgCTCTTCATCATTATTTTCGCTGTCATTCTGCTGACCAGGAAACGGGTGAACCTCAATGGca < 1:2540856/65‑1 (MQ=255) gCTCTTCATCATTATTTTCGCTGTCATTCTGCTGACCAGGAAACGGGTGAACCTCAATGGcaaca > 1:2049585/1‑65 (MQ=255) tctTCATCATTATTTTCGCTGTCATTCTGCTGACCAGGAAACGGGTGAACCTCAATGGCAACaaa > 1:1110655/1‑65 (MQ=255) tctTCATCATTATTTTCGCTGTCATTCTGCTGACCAGGAAACGGGTGAACCTCAATGGCAACaaa > 1:2107156/1‑65 (MQ=255) tctTCATCATTATTTTCGCTGTCATTCTGCTGACCAGGAAACGGGTGAACCTCAATGGCAACaaa > 1:2043374/1‑65 (MQ=255) tctTCATCATTATTTTCGCTGTCATTCTGCTGACCAGGAAACGGGTGAACCTCAATGGCAACaaa > 1:926678/1‑65 (MQ=255) | TATCTCTACAAAACCGCCTTTGCCTTTAATGATTTAGGAAAAGCGGCGGCGATCTCGGTGGTGCTCTTCATCATTATTTTCGCTGTCATTCTGCTGACCAGGAAACGGGTGAACCTCAATGGCAACAAA > NC_000913/1373972‑1374100 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |