| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,374,039 | 0 | T | G | 25.0% | 38.8 / 12.8 | 20 | F273C (TTC→TGC) | ycjO | putative ABC transporter membrane subunit YcjO |
| Reads supporting (aligned to +/- strand): ref base T (8/7); new base G (0/5); total (8/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.47e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.01e-01 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TCTACAAAACCGCCTTTGCCTTTAATGATTTAGGAAAAGCGGCGGCGATCTCGGTGGTGCTCTTCATCATTATTTTCGCTGTCATTCTGCTGACCAGGAAACGGGTGAACCTCAATGGCAACAAA > NC_000913/1373976‑1374100 | tctACAAAACCGCCTTTGCCTTTAATGATTTAGGAAAAGCGGCGGCGGTCTCGGGGGGGCTTTtc > 1:1703201/1‑65 (MQ=255)tctACAAAACCGCCTTTGCCTTTAATGATTTAGGAAAAGCGGCGGCGATCTCGGTGGTGCTCTtc < 1:2800701/65‑1 (MQ=255) ctACCAAACCGCCTTTGCCTTTAATGATTTAGGAAAAGCGGCGGCGATCTCGGTGGTGCTCTtca < 1:1317849/65‑1 (MQ=255) aCCGCCTTTGCCTTTAATGATTTAGGAAAAGCGGCGGCGGTCTCGGTGGTGCTCTTCATCattat > 1:1172573/1‑65 (MQ=255) aCCGCCTTTGCCTTTAATGATTTAGGAAAAGCGGCGGCGGGCTCGGTGGGGCTCTTCATCagtat > 1:3028620/1‑65 (MQ=255) aCCGCCTTTGCCTTTAATGATTTAGGAAAAGCGGCGGCGGAATCGGTGGCTTTTTTCATCatttt > 1:658114/1‑63 (MQ=255) aCCGCCTTTGCCTTTAATGATTTAGGAAAAGCGGCGGCGATCTCGGTGGGGCTCTTCATCattat > 1:756964/1‑65 (MQ=255) ccGCCTTTGCCTTTAATGATTTAGGAAAAGCGGCGGCGATCTCGGTGGTGCTCTTCATCattatt < 1:551574/65‑1 (MQ=255) ctttgcctttAATGATTTAGGAAAAGCGGCGGCGATCTCGGTGGTGCTCTTCATCATTATTTTCg > 1:1875703/1‑65 (MQ=255) gcctttAATGATTTGGGAGAAGGGGTGTAGATCTCGGTGGT‑CGCCGTATCATTATTTTCGCTGTc < 1:783282/65‑1 (MQ=255) gcctttAATGATTTAGGAAAAGCGGCGGCGATCTCGGTGGTGCTCTTCATCATTATTTTCGCTGt < 1:1572020/65‑1 (MQ=255) tAGGAAAAGCGGCGGCGATCTCGGTGGTGCTCTTCATCATTATTTTCGCTGTCATTCTGCTGAcc < 1:329671/65‑1 (MQ=255) ggcgcgtcgATCTCGGTGGT‑CGCCGTATCATTATTTTCGCTGTCATTCTGCTGACCAGGAAACgg < 1:2376886/62‑1 (MQ=255) gcggcggcgATCTCGGTGGTGCTCTTCATCATTATTTTCGCTGTCATTCTGCTGACCAGGAAACg < 1:963523/65‑1 (MQ=255) ggcggcgATCTCGGTGGTGCTCTTCATCATTATTTTCGCTGTCATTCTGCTGACCAGGAAACggg < 1:1567794/65‑1 (MQ=255) tagATCTCGGTGGT‑CGCCGTATCATTATTTTCGCTGTCATTCTGCTGACCAGGAAACGGGTGAAc < 1:2428665/63‑1 (MQ=255) cgATCTCGGTGGT‑CGCCGTATCATTATTTTCGCTGTCATTCTGCTGACCAGGAAACGGGTGAAcc < 1:2703502/65‑1 (MQ=255) agATCTCGGTGGT‑CGCCGTATCATTATTTTCGCTGTCATTCTGCTGACCAGGAAACGGGTGAAcc < 1:1765329/64‑1 (MQ=255) gATCTCGGTGGTGCTCTTCATCATTATTTTCGCTGTCATTCTGCTGACCAGGAAACGGGTGAAcc < 1:2741376/65‑1 (MQ=255) gtggtgCTCTTCATCATTATTTTCGCTGTCATTCTGCTGACCAGGAAACGGGTGAACCTCAATgg > 1:22714/1‑65 (MQ=255) gtggtgCTCTTCATCATTATTTTCGCTGTCATTCTGCTGACCAGGAAACGGGTGAACCTCAATgg > 1:360425/1‑65 (MQ=255) gtggtgCTCTTCATCATTATTTTCGCTGTCATTCTGCTGACCAGGAAACGGGTGAACCTCAATgg > 1:1811831/1‑65 (MQ=255) ggtgCTCTTCATCATTATTTTCGCTGTCATTCTGCTGACCAGGAAACGGGTGAACCTCAATGGca < 1:2540856/65‑1 (MQ=255) gCTCTTCATCATTATTTTCGCTGTCATTCTGCTGACCAGGAAACGGGTGAACCTCAATGGcaaca > 1:2049585/1‑65 (MQ=255) tctTCATCATTATTTTCGCTGTCATTCTGCTGACCAGGAAACGGGTGAACCTCAATGGCAACaaa > 1:2107156/1‑65 (MQ=255) tctTCATCATTATTTTCGCTGTCATTCTGCTGACCAGGAAACGGGTGAACCTCAATGGCAACaaa > 1:2043374/1‑65 (MQ=255) tctTCATCATTATTTTCGCTGTCATTCTGCTGACCAGGAAACGGGTGAACCTCAATGGCAACaaa > 1:926678/1‑65 (MQ=255) tctTCATCATTATTTTCGCTGTCATTCTGCTGACCAGGAAACGGGTGAACCTCAATGGCAACaaa > 1:1110655/1‑65 (MQ=255) | TCTACAAAACCGCCTTTGCCTTTAATGATTTAGGAAAAGCGGCGGCGATCTCGGTGGTGCTCTTCATCATTATTTTCGCTGTCATTCTGCTGACCAGGAAACGGGTGAACCTCAATGGCAACAAA > NC_000913/1373976‑1374100 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |