Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,282,3310TG55.6% ‑6.5 / 10.7 9V567G (GTG→GGG) radDputative DNA repair helicase RadD
Reads supporting (aligned to +/- strand):  ref base T (0/4);  new base G (5/0);  total (5/4)
Fisher's exact test for biased strand distribution p-value = 7.94e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.17e-02
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

TGCGACACCCGGATTTTGTCGTCGCCCGCATGAAAGGCCAGTACTGGCAGGTGCGTGAAAAAGTGTTCGATTACGAAGGTCGTTTTCGTCTGGCGCACGAATTACGCGGTTAATG  >  NC_000913/2282280‑2282394
                                                   |                                                               
tGCGACACCCGGATTTTGTCGTCGCCCGCATGAAAGGCCAGTACTGGCAGGTGTGTGGAAAAgag                                                    >  1:2732512/1‑63 (MQ=255)
tGCGACACCCGGATTTTGTCGTCGCCCGCATGAAAGGCCAGTACTGGCAGGGGGGGGaaaaaagg                                                    >  1:1988982/1‑62 (MQ=255)
      acCCGGATTTTGTCGTCGCCCGCATGAAAGGCCAGTACTGGCAGGGGCGTGAAAAAGAGGTCGAt                                              >  1:1283821/1‑65 (MQ=255)
              tttGTCGTCGCCCGCATGAAAGGCCAGTACTGGCAGGGGCGGGGAAAAGAGTTCTATTTCGAAgg                                      >  1:2151861/1‑65 (MQ=255)
              tttGTCGTCGCCCGCATGAAAGGCCAGTACTGGCAGGGGCGGGGAAAAGAGTTCGCTTACGAAgg                                      >  1:2079941/1‑65 (MQ=255)
                             aTGAAAGGCCAGTACTGGCAGGTGCGTGAAAAAGTGTTCGATTACGAAGGTCGTTTTCGTCTGgc                       <  1:93408/65‑1 (MQ=255)
                                  aGGCCAGTACTGGCAGGGGCGGGGAAAAAGGTTCGATTACGAAGGGCGGTTTTGTCTGgcgccag                  >  1:3020978/1‑62 (MQ=255)
                                   ggCCAGTACTGGCAGGGGCGTGAAAAAGGGTTCGATTACGAAGGCCGTTTTCGTCTGGCGCACGa                 >  1:115692/1‑65 (MQ=255)
                                   ggCCAGTACTGGCAGGGGCGGGGAAAAGGGTTTGAGTAAGAAGGCGGTTTTAGTCTGGGGCACgg                 >  1:74873/1‑64 (MQ=255)
                                   ggCCAGTACTGGCAGGGGCGGGGAAAAGAGTTCGATTACGAAGGGCGTTTTTGTCTGGGGCACGa                 >  1:9530/1‑65 (MQ=255)
                                     ccAGTACTGGCAGGGGCGGGAAAAAAAGGTCGATTACGAAGGGCGGTTTTGTCTGGGGCCCGGAt               >  1:321808/1‑65 (MQ=255)
                                             ggCTGGTGCGTGAAAAAGTGTTCGATTACGAAGGTCGTTTTCGTCTGGCGCACGAATTACGCGGt       <  1:1302449/65‑1 (MQ=255)
                                             ggCAGGTGCGTGAAAAAGTGTTCGATTACGAAGGTCGTTTTCGTCTGGCGCACGAATTACGCGGt       <  1:3009256/65‑1 (MQ=255)
                                             ggCAGGTGCGTGAAAAAGTGTTCGATTACGAAGGTCGTTTTCGTCTGGCGCACGAATTACGCGGt       <  1:3159205/65‑1 (MQ=255)
                                             ggCAGGTGCGTGAAAAAGTGTTCGATTACGAAGGTCGTTTTCGTCTGGCGCACGAATTACGCGGt       <  1:673012/65‑1 (MQ=255)
                                             ggCAGGTGCGTGAAAAAGTGTTCGATTACGAAGGTCGTTTTCGTCTGGCGCACGAATTACGCGGt       <  1:2393029/65‑1 (MQ=255)
                                             ggCAGGTGCGTGAAAAAGTGTTCGATTACGAAGGTCGTTTTCGTCTGGCGCACGAATTACGCGGt       <  1:1451319/65‑1 (MQ=255)
                                                  gTGCGTGAAAAAGTGTTCGATTACGAAGGTCGTTTTCGTCTGGCGCACGAATTACGCGGTTAATg  <  1:3072977/65‑1 (MQ=255)
                                                  gTGCGTGAAAAAGTGTTCGATTACGAAGGTCGTTTTCGTCTGGCGCACGAATTACGCGGTTAATg  <  1:356404/65‑1 (MQ=255)
                                                   |                                                               
TGCGACACCCGGATTTTGTCGTCGCCCGCATGAAAGGCCAGTACTGGCAGGTGCGTGAAAAAGTGTTCGATTACGAAGGTCGTTTTCGTCTGGCGCACGAATTACGCGGTTAATG  >  NC_000913/2282280‑2282394

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑