Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	49,765	C→T	intergenic (+134/‑58)	kefC → / → folA	K(+) : H(+) antiporter KefC/dihydrofolate reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	49,765	0	C	T	100.0%	154.6 / NA	43	intergenic (+134/‑58)	kefC/folA	K(+) : H(+) antiporter KefC/dihydrofolate reductase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (25/18);  total (25/18)

GGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCA  >  NC_000913/49703‑49829                                                               |                                                                 ggAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTtga                                                                >  1:761475/1‑65 (MQ=255) ggAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTtga                                                                >  1:75713/1‑65 (MQ=255) ggAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTtga                                                                >  1:111947/1‑65 (MQ=255) ggAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTtga                                                                >  1:356905/1‑65 (MQ=255) ggAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCATCGCACCAGTtga                                                                >  1:2712113/1‑65 (MQ=255)   agagagCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTtgacg                                                              >  1:915561/1‑65 (MQ=255)    gagagCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTtgacga                                                             >  1:3131773/1‑65 (MQ=255)    gagagCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTtgacga                                                             >  1:2882972/1‑65 (MQ=255)     agagCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTgacgac                                                            >  1:2043190/1‑65 (MQ=255)     agagCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTgacgac                                                            >  1:2105108/1‑65 (MQ=255)     agagCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTCgacgac                                                            >  1:3014440/1‑65 (MQ=255)       agCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGACGACgg                                                          <  1:568194/65‑1 (MQ=255)       agCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGACGACgg                                                          <  1:2337233/65‑1 (MQ=255)       agCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGACGACgg                                                          >  1:2225384/1‑65 (MQ=255)       agCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGACGACgg                                                          <  1:873470/65‑1 (MQ=255)        gCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGACGACGGt                                                         <  1:3002159/65‑1 (MQ=255)             gACTCGCCAGCAGAATATAAACTTTTCCTCAACATCATCCTCGCACCAGTTGACGACGGTTTACg                                                    >  1:145600/1‑65 (MQ=255)             gACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGACGACGGTTTACg                                                    >  1:1682279/1‑65 (MQ=255)              aCTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGACGACGGTTTACGc                                                   >  1:206793/1‑65 (MQ=255)              aCTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGACGACGGTTTACGc                                                   >  1:18780/1‑65 (MQ=255)              aCTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGACGACGGTTTACGc                                                   >  1:535459/1‑65 (MQ=255)              aCTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGACGACGGTTTACGc                                                   >  1:2466785/1‑65 (MQ=255)              aCTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGACGACGGTTTACGc                                                   <  1:1574605/65‑1 (MQ=255)              aCTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGACGACGGTTTACGc                                                   >  1:1642885/1‑65 (MQ=255)               cTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGACGACGGTTTACGCt                                                  <  1:599693/65‑1 (MQ=255)               cTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGACGACGGTTTACGCt                                                  <  1:475925/65‑1 (MQ=255)                   ccAGCAGAATATAAAATTTTCCTCAACGTCATCCTCGCACCAGTTGACGACGGTTTACGCTTTAc                                              <  1:610579/65‑1 (MQ=255)                   ccAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGACGACGGTTTACGCTTTAc                                              <  1:2581403/65‑1 (MQ=255)                   ccAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGACGACGGTTTACGCTTTAc                                              <  1:353408/65‑1 (MQ=255)                   ccAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGACGACGGTTTACGCTTTAc                                              <  1:3088929/65‑1 (MQ=255)                     agcagAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGACGACGGTTTACGCTTTACGt                                            <  1:2279664/65‑1 (MQ=255)                      gcagAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGACGACGGTTTACGCTTTACGta                                           >  1:1433911/1‑65 (MQ=255)                        agAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGACGACGGTTTACGCTTTACGtata                                         >  1:509293/1‑65 (MQ=255)                        agAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGACGACGGTTTACGCTTTACGtata                                         >  1:2873194/1‑65 (MQ=255)                        agAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGACGACGGTTTACGCTTTACGtata                                         >  1:3103830/1‑65 (MQ=255)                        agAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGACGACGGTTTACGCTTTACGtata                                         >  1:1333124/1‑65 (MQ=255)                        agAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGACGACGGTTTACGCTTTACGtata                                         >  1:2911620/1‑65 (MQ=255)                        agAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGACGACGGTTTACGCTTTACGtata                                         >  1:2339441/1‑65 (MQ=255)                        aaaaTATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGACGACGGTTTACGCTTTACGtata                                         >  1:58680/3‑65 (MQ=255)                           atatAAAATTTTCCTCAACATCATCCTCGCACCAGTTGACGACGGTTTACGCTTTACGTATAGTg                                      >  1:2483257/1‑65 (MQ=255)                            tataAAATTTTCCTCAACATCATCCTCGCACCAGTTGACGACGGTTTACGCTTTACGTATAGTgg                                     <  1:884821/65‑1 (MQ=255)                             ataAAATTTTCCTCAACATCATCCTCGCACCAGTTGACGACGGTTTACGCTTTACGTATAGTGGc                                    >  1:2513786/1‑65 (MQ=255)                             ataAAATTTTCCTCAACATCATCCTCGCACCAGTTGACGACGGTTTACGCTTTACGTATAGTGGc                                    >  1:578511/1‑65 (MQ=255)                                aaaTTTTCCTCAACATCATCCTCGCACCAGTTGACGACGGTTTACGCTTTACGTATAGTGGCGAc                                 >  1:2413725/1‑65 (MQ=255)                                              atcatcCTCGCACCAGTTGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATcc                   <  1:436823/65‑2 (MQ=255)                                                   ccTCGCACCTGTTGACGACGGTTTGCGCTTTCCGTATAGTGGCGACAATTTTTTTTATCGGGaaa              <  1:2241949/65‑1 (MQ=255)                                                   ccTCGCACCAGTTGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGgaa              >  1:1419850/1‑65 (MQ=255)                                                   ccTCGCACCAGTTGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATAGGGaaa              >  1:2807210/1‑65 (MQ=255)                                                    cTCGCACCAGTTGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATTGGGAAAt             >  1:2049364/1‑65 (MQ=255)                                                    cTCGCACCAGTTGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAAt             >  1:1618157/1‑65 (MQ=255)                                                    cTCGCACCAGTTGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTCTTTATCGGGAAAt             >  1:1427543/1‑65 (MQ=255)                                                     tCGCACCAGTTGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAAtc            <  1:896634/65‑1 (MQ=255)                                                     tCGCACCAGTTGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAAtc            <  1:3058827/65‑1 (MQ=255)                                                       gCACCAGTTGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAAtctc          <  1:1177099/65‑1 (MQ=255)                                                          ccAGTTGACGACGGTTTACGCTTTACGTATGGTGGCGACAATTTTTTTTATCGGGAAATCTCAAt       <  1:417565/65‑1 (MQ=255)                                                               cgacgaCGGTTTACGCTTTACGTATAATGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCa  >  1:3033680/1‑65 (MQ=255)                                                               |                                                                 GGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCA  >  NC_000913/49703‑49829

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑