| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 4,390,754 | G→T | A252A (GCC→GCA) | rsgA ← | ribosome small subunit‑dependent GTPase A |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,390,754 | 0 | G | T | 95.5% | 72.0 / NA | 22 | A252A (GCC→GCA) | rsgA | ribosome small subunit‑dependent GTPase A |
| Reads supporting (aligned to +/- strand): ref base G (0/1); new base T (14/7); total (14/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.64e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ACTCACGCACTCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAG‑TGATACAGCCGAGCGGCGGTCGTGGTGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGATCTC > NC_000913/4390693‑4390813 | aCTCACGCACTCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAG‑TGATACAGCCGAgctgcg > 1:2893389/1‑65 (MQ=255) tCACGCACTCCTGGGGAGTCAATCACTTCCCCGCCGTGCGGGAAG‑TGATACAGCCGAGCTGCGGt < 1:847715/65‑1 (MQ=255) tCACGCACTCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAG‑TGATACAGCCGAGCTGCGGt < 1:1041365/65‑1 (MQ=255) tCACGCACTCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAG‑TGATACAGCCGAGCTGCGGt < 1:886054/65‑1 (MQ=255) tCACGCACTCCTGGGGAGTCAATCACATCACCGCCGTGCCGGAAG‑TGATACAGCCGAGCTGCGGt > 1:3184098/1‑65 (MQ=255) tCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAG‑TGATACAGCCGAGCTGCGGTCGTGgtgt < 1:2771027/65‑1 (MQ=255) ggggAGTCAATCACATCACCGCCGTGCGGGAAG‑TGATACAGCCGAGCTGCGGTCGTGGTGTGCTg > 1:1453432/1‑65 (MQ=255) aGTCAATCACATCACCGCCGTGCGGGAAG‑TGATACAGCCGAGCTGCGGTCGTGGTGTGCTGAccc > 1:1487032/1‑64 (MQ=255) aGTCAATCACATCACCGCCGTGCGGGAAG‑TGATACAGCCGAGCTGCGGTCGTGGTGTGCTGAccc > 1:2688652/1‑64 (MQ=255) aGTCAATCACATCACCGCCGTGCGGGAAG‑TGATACAGCCGAGCTGCGGTCGTGGTGTGCTGACCg > 1:1796862/1‑65 (MQ=255) aGTCAATCACATCACCGCCGTGCGGGAAG‑TGATACAGCCGAGCTGCGGTCGTGGTGTGCTGACCg > 1:817074/1‑65 (MQ=255) aGTCAATCACATCACCGCCGTGCGGGAAG‑TGATACAGCCGAGCTGCGGTCGTGGTGTGCTGACCg > 1:1988799/1‑65 (MQ=255) aGTCAATCACATCACCGCCGTGCGGGAAG‑TGATACAGCCGAGCTGCGGTCGTGGTGTGCTGACCg > 1:291383/1‑65 (MQ=255) aGTCAATCACATCACCGCCGTGCGGGAAG‑TGATACAGCCGAGCTGCGGTCGTGGAGTGCTGACCg > 1:1633186/1‑65 (MQ=255) gccgTGCGGGAAG‑TGATACAGCCGAGCTGCGGTCGTGGTGTGCTGACCGAGCCCCGAGTTGTCag > 1:138826/1‑65 (MQ=255) ccgTGCGGGAAG‑TGATACAGCCGAGCGGCGGTCGTGGTGTGCTGACCGAGCCCCGAGTTGTCaga < 1:1696188/65‑1 (MQ=255) gggAAG‑TGATACAGCCGAGCTGCGGTCGTGGTGTGCTGACCGAGCCCCGAGTTGTCAGAGATATc < 1:2032840/65‑1 (MQ=255) ggAAGTTGATACAGCCGAGCTGCGGTCGTGGTGTGCTGACCGAGCCCCGAGTTGTCAGAGATATc < 1:2660549/65‑1 (MQ=255) g‑TGATACAGCCGAGCTGCGGTCGTGGTGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGCTgt > 1:2997655/1‑64 (MQ=255) aTACAGCCGAGCTGCGGTCGTGGTGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCa < 1:491889/65‑1 (MQ=255) tACAGCCGAGCTGCGGTCGTGGTGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAg < 1:2270926/65‑1 (MQ=255) agtgCCGAGCTGCGGTCGTGGTGGGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAgg < 1:3056225/62‑1 (MQ=255) aCAGCCGAGCTGCGGTCGTGGTGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAgg > 1:2862421/1‑65 (MQ=255) aCAGCCGAGCTGCGGTCGTGGTGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAgg > 1:1973862/1‑65 (MQ=255) aCAGCCGAGCTGCGGTCGTGGGGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGCCAgg > 1:722130/1‑65 (MQ=255) gCCGAGCTGCGGTCGTGGTGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGAtc > 1:2958795/1‑65 (MQ=255) ggAGCTGCGGTCGTGGTGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGAtctc < 1:914625/64‑1 (MQ=255) cGAGCTGCGGTCGTGGTGTGCTGGCCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGAtctc < 1:1824874/65‑1 (MQ=255) cGAGCTGCGGTCGTGGTGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGAtctc > 1:945671/1‑65 (MQ=255) | ACTCACGCACTCCTGGGGAGTCAATCACATCACCGCCGTGCGGGAAG‑TGATACAGCCGAGCGGCGGTCGTGGTGTGCTGACCGAGCCCCGAGTTGTCAGAGATATCGTTGGTCAGGATCTC > NC_000913/4390693‑4390813 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |