Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	49,910	T→A	W30R (TGG→AGG)	folA →	dihydrofolate reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	49,910	0	T	A	100.0%	79.5 / NA	23	W30R (TGG→AGG)	folA	dihydrofolate reductase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (14/9);  total (14/9)

GTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGG  >  NC_000913/49850‑49964                                                             |                                                       gTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTACCGATCTCGCCAGGtt                                                    >  1:269506/1‑65 (MQ=255)    gATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTACCGATCTCGCCAGGTTTaa                                                 <  1:12390/65‑1 (MQ=255)     aTCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCGCGCCAGGGTTcaa                                                >  1:2450573/1‑65 (MQ=255)     aTCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCCACCGCTCTCGCCAGGGTTaaa                                                >  1:2807118/1‑65 (MQ=255)     aTCGCGTTATCGGCATGGAAAAAGCCATGCCGGGGAACCCGCCTACCGATCTCGCCAGGTTTaaa                                                >  1:1231428/1‑65 (MQ=255)      tCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTACCGATCTCGCCAGGTTTAAAc                                               >  1:2312358/1‑65 (MQ=255)        gcgTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTACCGATCTCGCCAGGTTTAAACGc                                             >  1:449771/1‑65 (MQ=255)            tATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTACCGATCTCGCCAGGTTTAAACGCAaca                                         >  1:3005932/1‑65 (MQ=255)             aTCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTACCGATCTCGCCAGGTTTAAACGCAacac                                        <  1:300934/65‑1 (MQ=255)             aTCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTACCGATCTCGCCAGGTTTAAACGCAacac                                        <  1:369262/65‑1 (MQ=255)             aTCGGCATGGAAAACGCCATGCCGGGGAACCTGCCTGCCGATCGCGCCAGGTTTAAACGCAacac                                        <  1:554746/65‑1 (MQ=255)              tccgCATGGAAAACGCCATGCCGTGGAACCTGCCTACCGATCTCGCCAGGTTTAAACGCAACAcc                                       <  1:217777/62‑1 (MQ=255)              tCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTACCGATCTCGCCAGGTTTAAACGCAACAcc                                       <  1:1684096/65‑1 (MQ=255)              tCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTACCGATCTCGCCAGGTTTAAACGCAACAcc                                       <  1:2766016/65‑1 (MQ=255)                 gCATGGAAAACGCCATGCCGTGGACCCTGCCTACCGATCTCGCCAGGTTTAAACGCAACACCTta                                    <  1:1617691/65‑1 (MQ=255)                  cATGGAAAACGCCATGCCGTGGAACCTGCCTACCGATCTCGCCAGGTTTAAACGCAACACCTtaa                                   <  1:698083/65‑1 (MQ=255)                       aaaaCGCCATGCCGTGGAACCTGCCTACCGATCTCGCCAGGTTTAAACGCAACACCTtaaataaa                              >  1:297565/1‑65 (MQ=255)                           cGCCATGCCGTGGAACCTGCCTACCGATCTCGCCAGGTTTAAACGCAACACCTTAAATAAACCCg                          >  1:1050943/1‑65 (MQ=255)                            gCCATGCCGTGGAACCTGCCTACCGATCTCGCCAGGTTTAAACGCAACACCTTAAATAAACCCGt                         >  1:1522890/1‑65 (MQ=255)                            gCCATGCCGTGGAACCTGCCTACCGATCTCGCCAGGTTTAAACGCAACACCTTAAATAAACCCGt                         >  1:851175/1‑65 (MQ=255)                                  ccGTGGAACCTGCCTACCGATCTCGCCAGGTTTAAACGCAACACCTTAAATAAACCCGTGCTTAt                   >  1:1426974/1‑65 (MQ=255)                                        aaCCTGCCTACCGATCTCGCCAGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGgccg             >  1:2275479/1‑65 (MQ=255)                                         aCCTGCCTACCGATCTCGCCAGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGgccgc            >  1:695492/1‑65 (MQ=255)                                         aCCTGCCTACCGATCTCGCCAGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGgccgc            >  1:2452010/1‑65 (MQ=255)                                         aCCTGCCTACCGATCTCGCCAGGTTTAAACGCAACACCCTAAATAAACCCGTGATTATGGgccgc            >  1:1920070/1‑65 (MQ=255)                                           ctgcctacCGATCTCGCCAGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGgccgcta          >  1:1124728/1‑63 (MQ=255)                                                 accGATCTCGCCAGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTg    >  1:561566/2‑65 (MQ=255)                                                   cGATCTCGCCAGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTggg  >  1:2609643/1‑65 (MQ=255)                                                             |                                                       GTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGG  >  NC_000913/49850‑49964

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑