Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	50,102	A→C	I94L (ATT→CTT)	folA →	dihydrofolate reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	50,102	0	A	C	100.0%	135.8 / NA	41	I94L (ATT→CTT)	folA	dihydrofolate reductase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (29/12);  total (29/12)

CGTGGGTGAAGTCGGTGGATGAAGCCATCGCGGCGTGTGGTGACGTACCAGAAATCATGGTGATTGGCGGCGGTCGCGTTTATGAACAGTTCTTGCCAAAAGCGCAAAAACTGTATCTGACGCA  >  NC_000913/50040‑50163                                                               |                                                              cGTGGGTGAAGTCGGTGGATGAAGCCATCGCGGCGTGTGGTGACGTACCAGAAATCATGGTGCtt                                                             >  1:442624/1‑65 (MQ=255) cGTGGGTGAAGTCGGTGGATGAAGCCATCGCGGCGTGTGGTGACGTACCAGAAATCATGGTGCtt                                                             >  1:699288/1‑65 (MQ=255)  gTGGGTGAAGTCGGTGGATGAAGCCATCGCGGCGTGTGGTGACGTACCAGAAATCATGGTGCTTg                                                            >  1:3040292/1‑65 (MQ=255)         aaGTCGGTGGATGAAGCCATCGCGGCGTGTGGTGACGTACCAGAAATCATGGTGCTTGGcggcgg                                                     <  1:317840/65‑1 (MQ=255)           gTCGGTGGATGAAGCCATCGCGGCGTGTGGTGACGTACCAGAAATCATGGTGCTTGGCGGCGGTc                                                   >  1:1225778/1‑65 (MQ=255)            tCGGTGGATGAAGCCATCGCGGCGTGTGGTGACGTACCAGAAATCATGGTGCTTGGCGGCTGTct                                                  >  1:828129/1‑64 (MQ=39)            tCGGTGGATGAAGCCATCGCGGCGTGTGGTGACGTACCAGAAATCATGGTGCTTGGCGGCGGTcg                                                  >  1:2533715/1‑65 (MQ=255)            tCGGTGGATGAAGCCATCGCGGCGTGTGGTGACGTACAAGAAATCATGGTGCTTGGCGGCGGTcg                                                  >  1:2010118/1‑65 (MQ=255)            tCGGTGGATGAAGCCACCGCGGCGTGTGGTGACGTACCAGAAATCATGGCGCTTGGCGGCGGCcg                                                  >  1:1366500/1‑65 (MQ=37)                  gATGAAGCCATCGCGGCGTGTGGTGACGTACCAGAAATCATGGTGCTTGGCGGCGGTCGCGTTTa                                            >  1:867444/1‑65 (MQ=255)                    tGAAGCCATCGCGGCGTGTGGTGACGTACCAGAAATCATGGTGCTTGGCGGCGGTCGCGTTTATg                                          >  1:2735069/1‑65 (MQ=255)                    tGAAGCCATCGCGGCGTGTGGTGACGTACCAGAAATCATGGTGCTTGGCGGCGGTCGCGTTTATg                                          >  1:1909003/1‑65 (MQ=255)                     gAAGCCATCGCGGCGTGTGGTGACGTACCAGAAATCATGGTGCTTGGCGGCGGTCGCGTTTATGa                                         <  1:661703/65‑1 (MQ=255)                     gAAGCCATCGCGGCGTGTGGTGACGTACCAGAAATCATGGTGCTTGGCGGCGGTCGCGTTTATGa                                         <  1:1904901/65‑1 (MQ=255)                      aaGCCATCGCGGCGTGTGGTGACGTACCAGAAATCATGGTGCTTGGCGGCGGTCGGGTTTATGaa                                        >  1:685928/1‑65 (MQ=255)                        gCCATCGCGGCGTGTGGTGACGTACCAGAAATCATGGTGCTTGGCGGCGGTCGCGTTTATGAACa                                      >  1:151078/1‑65 (MQ=255)                        gCCATCGCGGCGTGTGGTGACGTACCAGAAATCATGGTGCTTGGCGGCGGTCGCGTTTATGAACa                                      <  1:2235537/65‑1 (MQ=255)                         ccATCGCGGCGTGTGGTGACGTACCAGAAATCATGGTGCTTGGCGGCGGTCGCGTTTATGACCAg                                     >  1:1025000/1‑65 (MQ=255)                         ccATCGCGGCGTGTGGTGACGTACCAGAAATCATGGTGCTTGGCGGCGGTCGCGTTTATGAACAg                                     >  1:956078/1‑65 (MQ=255)                         ccATCGCGGCGTGTGGTGACGTACCAGAAATCATGGTGCTTGGCGGCGGTCGCGTTTATGAACAg                                     >  1:3231490/1‑65 (MQ=255)                         ccATCGCGGCGTGTGGTGACGTACCAGAAATCATGGTGCTTGGCGGCGGTCGCGGCTATGAAAAg                                     >  1:1804784/1‑65 (MQ=37)                         ccATCGCGGCGTGTGGTGACGTACCAGAAATCATGGCGCTTGGCGGCGGTCGCGTTTATGAACAg                                     >  1:2535514/1‑65 (MQ=255)                          cATCGCGGCGTGTGGTGACGTACCAGAAATCATGGTGCTTGGCGGCGGTCGCGTTTATGAACAGt                                    <  1:1871153/65‑1 (MQ=255)                          cATCGCGGCGTGTGGTGACGTACCAGAAATCATGGTGCTTGGCGGCGGTCGCGTTTATGAACAGt                                    <  1:1276273/65‑1 (MQ=255)                          cATCGCGGCGTGTGGTGACGTACCAGAAATCATGGTGCTTGGCGGCGGTCGCGTTTATGAACAGt                                    <  1:2229039/65‑1 (MQ=255)                          cATCGCGGCGTGTGGTGACGTACCAGAAATCATGGTGCTTGGCGGCGGTCGCGTTTATGAACAGt                                    <  1:1446832/65‑1 (MQ=255)                             cgcgGCGTGTGGTGACGTACCAGAAATCATGGTGCTTGGCGGCGGTCGCGTTTATGAACAGTTCt                                 <  1:18953/65‑1 (MQ=255)                              gcggcgTGTGGTGACGTACCAGAAATCATGGTGCTTGGCGGCGGTCGCGTTTATGAACAGTTCtt                                >  1:1990762/1‑65 (MQ=255)                                  cgTGTGGTGACGTACCAGAAATCATGGTGCTTGGCGGCGGTCGCGTTTATGGACAGTTCTTGCCa                            >  1:2678892/1‑65 (MQ=39)                                  cgTGTGGTGACGTACCAGAAATCATGGTGCTTGGCGGCGGTCGCGTTTATGAACAGTTCTTGCCa                            >  1:320087/1‑65 (MQ=255)                                     gtggtgACGTACCAGAAATCATGGTGCTTGGCGGCGGTCGCGTTTATGAACAGTTCTTGCCaaaa                         >  1:2550462/1‑65 (MQ=255)                                     gtggtgACGTACCAGAAATCATGGTGCTTGGCGGCGGGCGCGTTTATGAACAGTTCTTGCCaaaa                         >  1:1660290/1‑65 (MQ=39)                                          gACGTACCAGAAATCATGGTGCTTGGCGGCGGTCGCGTTTATGAACAGTTCTTGCCAAAAGCGCa                    >  1:159684/1‑65 (MQ=255)                                          gACGTACCAGAAATCATGGTGCTTGGCGGCGGTCGCGTTTATGAACAGTTCTTGCCAAAAGCGCa                    >  1:2186613/1‑65 (MQ=255)                                          gACGTACCAGAAATCATGGTGCTTGGCGGCGGTCGCGTTTATGAACAGTTCTTGCCAAAAGCGCa                    >  1:280734/1‑65 (MQ=255)                                          gACGTACCAGAAATCATGGTGCTTGGCGGCGGTCGCGTTTATGAACAGTTCTTGCCAAAAGCGCa                    >  1:2882116/1‑65 (MQ=255)                                            cGTACCAGAAATCATGGTGCTTGGCGGCGGTCGCGTTTATGAACAGTTCTTGCCAAAAGCGCaaa                  >  1:1052952/1‑65 (MQ=255)                                            cGTACCAGAAATCATGGTGCTTGGCGGCGGTCGCGTTTATGAACAGTTCTTGCCAAAAGCGCaaa                  >  1:261108/1‑65 (MQ=255)                                            cGTACCAGAAATCATGGTGCTTGGCGGCGGTCGCGTTTATGAACAGTTCTTGCCAAAAGCGCaaa                  >  1:1482098/1‑65 (MQ=255)                                              tACCAGAAATCATGGTGCTTGGCGGCGGTCGCGTTTATGAACAGTTCTTGCCAAAAGCGCaaaaa                <  1:138957/65‑1 (MQ=255)                                              tACCAGAAATCATGGTGCTTGGCGGCGGTCGCGTTTATGAACAGTTCTTGCCAAAAGCGCaaaaa                <  1:1113335/65‑1 (MQ=255)                                               aCCAGAAATCATGGTGCTTGGCGGCGGTCGCGTTTATGAACAGTTCTTGCCAAAAGCGCAAAAAc               >  1:2904703/1‑65 (MQ=255)                                               aCCAGAAATCATGGTGCTTGGCGGCGGTCGCGTTTATGAACAGTTCTTGCCAAAAGCGCAAAAAc               >  1:564281/1‑65 (MQ=255)                                                   gAAATCATGGTGCTTGGCGGCGGTCGCGGTTATGAACAGTTCTTGCCAAAAGCGCAAAAACTGTa           >  1:2343550/1‑65 (MQ=255)                                                        cATGGTGCTTGGCGGCGGTCGCGTTTATGAACAGTTCTTGCCAAAAGCGCAAAAACTGTATCTGa      <  1:2034249/65‑1 (MQ=255)                                                           ggTGCTTGGCGGCGGTCGCGTTTATGAACAGTTCTTGCCAAAAGCGCAAAAACTGTATCTGACGc   >  1:1380060/1‑65 (MQ=255)                                                           ggTGATTGGCGGCGGTCGCGTTTATGAACAGTTCTTGCCAAAAGCGCAAAAACTGTATCTGACGc   <  1:2469887/65‑1 (MQ=255)                                                            gTGCTTGGCGGCGGTCGCGTTTATGAACAGTTCTTGCCAAAAGCGCAAAAACTGTATCTGACGCa  >  1:3152245/1‑65 (MQ=255)                                                               |                                                              CGTGGGTGAAGTCGGTGGATGAAGCCATCGCGGCGTGTGGTGACGTACCAGAAATCATGGTGATTGGCGGCGGTCGCGTTTATGAACAGTTCTTGCCAAAAGCGCAAAAACTGTATCTGACGCA  >  NC_000913/50040‑50163

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑