Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,905,761	G→A	G25D (GGT→GAT)	mntP →	Mn(2(+)) exporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,905,761	0	G	A	100.0%	120.1 / NA	34	G25D (GGT→GAT)	mntP	Mn(2(+)) exporter
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (14/20);  total (14/20)

TGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGGTGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGC  >  NC_000913/1905699‑1905821                                                               |                                                             tGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCAGTTTCTGCATCAATCGGTAAATGtc                                                            >  1:1745506/1‑64 (MQ=255)     tcTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGATGCCAc                                                        <  1:1192475/64‑1 (MQ=255)     gcTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGATGCCAc                                                        <  1:1630100/64‑1 (MQ=255)     aCTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGATGCCAc                                                        <  1:1949138/65‑1 (MQ=255)      cTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGATGCCAcc                                                       <  1:1703405/65‑1 (MQ=255)       tGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGATGCCAccc                                                      >  1:756831/1‑65 (MQ=255)       tGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGATGCCAccc                                                      >  1:1208013/1‑65 (MQ=255)       tGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGATGCCAccc                                                      >  1:599868/1‑65 (MQ=255)       tGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGATGCCAccc                                                      <  1:2828927/65‑1 (MQ=255)       tGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGATGCCAccc                                                      >  1:1480759/1‑65 (MQ=255)         ttcttcTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGATGCCACCCTc                                                    <  1:613642/65‑1 (MQ=255)         ttcttcTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGATGCCACCCTc                                                    <  1:1416096/65‑1 (MQ=255)          tcttctTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGATGCCACCCTcc                                                   <  1:1998472/65‑1 (MQ=255)          tcttctTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGATGCCACCCTcc                                                   <  1:2667512/65‑1 (MQ=255)          tcttctTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGATGCCACCCTcc                                                   <  1:2245700/65‑1 (MQ=255)               ttGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGATGCCACCCTCCATaaa                                              <  1:3045376/65‑1 (MQ=255)                        gTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGATGCCACCCTCCATAAACCGAAAttt                                     >  1:687527/1‑65 (MQ=255)                        gTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGATGCCACCCTCCATAAACCGAAAttt                                     >  1:369728/1‑65 (MQ=255)                         tATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGATGCCACCCTCCATAAACCGAAAtttt                                    >  1:3027129/1‑65 (MQ=255)                               gatggatgCATTTGCTGCATCAATCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAg                              >  1:2755531/1‑65 (MQ=255)                                  ggatgCATTTGCTGCATCAATCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCAt                           <  1:1424621/65‑1 (MQ=255)                                   gatgCATTTGCTGCATCAATCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCAtt                          >  1:1986829/1‑65 (MQ=255)                                   gatgCATTTGCTGCATCAATCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCAtt                          >  1:2780434/1‑65 (MQ=255)                                   gatgCATTTGCTGCATCAATCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCAtc                          >  1:1046559/1‑64 (MQ=255)                                        aTTTGCTGCATCAATCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGaa                     <  1:160808/65‑1 (MQ=255)                                         tttGCTGCATCAATCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAAc                    >  1:2488454/1‑65 (MQ=255)                                            gctgcATCAATCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCgg                 >  1:2179691/1‑65 (MQ=255)                                             ctgcATCAATCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGc                <  1:1983990/65‑1 (MQ=255)                                             ctgcATCAATCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGc                <  1:122381/65‑1 (MQ=255)                                             ctgcATCAATCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGc                <  1:411421/65‑1 (MQ=255)                                             ctgcATCAATCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGc                <  1:255184/65‑1 (MQ=255)                                              tgcATCAATCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGcc               >  1:68091/1‑65 (MQ=255)                                                 aTCAATCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGCCTTa            <  1:1779847/65‑1 (MQ=255)                                                 aTCAATCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGCCTTa            <  1:1118819/65‑1 (MQ=255)                                                  tCAATCGGTAAAGATGCCACCCTCCATAAACCGAATTTTTCTGAAGCATTGCGAACCGGCCTTAt           >  1:596023/1‑65 (MQ=255)                                                  tCAATCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGCCTTAt           <  1:1318174/65‑1 (MQ=255)                                                           aaaGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGc  >  1:119224/1‑65 (MQ=255)                                                               |                                                             TGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGGTGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGC  >  NC_000913/1905699‑1905821

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑