| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 481,400 | C→T | 100% | A1002T (GCA→ACA) | acrB ← | multidrug efflux pump RND permease AcrB |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 481,400 | 0 | C | T | 98.8% | 345.0 / ‑4.1 | 83 | A1002T (GCA→ACA) | acrB | multidrug efflux pump RND permease AcrB |
| Reads supporting (aligned to +/- strand): ref base C (1/0); new base T (44/38); total (45/38) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.76e-01 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
AAGCGGCGGCGAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAACCAGCGGCATAACGCCGAGGATAAACGCCAGCGAGGTCATCAGGATCGGACGTAAACG > NC_000913/481306‑481493 | aaGCGGCGGCGAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGTGTTc < 1:996754/99‑1 (MQ=255)aaGCGGCGGCGAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGTGTTc > 2:1031397/1‑99 (MQ=255)aaGCGGCGGCGAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGTGTTc > 1:1353585/1‑99 (MQ=255)aaGCGGCGGCGAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGTGTTc < 2:1455472/99‑1 (MQ=255)aaGCGGCGGCGAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGTGTTc < 2:2361516/99‑1 (MQ=255)aaGCGGCGGCGAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTc > 1:236656/1‑99 (MQ=255) gcggcggcgAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGTGTTCTg > 2:1437361/1‑99 (MQ=255) ggcgAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGTGTTCTgcgcgc > 1:489943/1‑99 (MQ=255) ggcgAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGTGTTCTgcgcgc < 2:530564/99‑1 (MQ=255) gcgAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGTGTTCTGCGCGcc < 1:1219004/99‑1 (MQ=255) gcgAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGTGTTCTGCGCGcc > 1:582721/1‑99 (MQ=255) aaCCACCACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGTGTTCTGCGCGCCGGa > 2:1843754/1‑99 (MQ=255) ccaccaCAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGTGTTCTGCGCGCCGGAAc > 1:809401/1‑99 (MQ=255) accacAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGTGTTCTGCGCGCCGGAACCa < 1:1537840/99‑1 (MQ=255) ccacAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGTGTTCTGCGCGCCGGAACCAg < 2:1609141/99‑1 (MQ=255) acaAAGAATACCGGAACGAAGAAGTTTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGTGTTCTGCGCGCCGGAACCAGCa < 1:476771/99‑1 (MQ=255) acaAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGTGTTCTGCGCGCCGGAACCAGCa < 1:625686/99‑1 (MQ=255) aaGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGTGTTCTGCGCGCCGGAACCAGCACCa < 1:2333399/99‑1 (MQ=255) aaGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGTGTTCTGCGCGCCGGAACCAGCACCa > 1:1565121/1‑99 (MQ=255) aaGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGTGTTCTGCGCGCCGGAACCAGCACCa > 1:1995231/1‑99 (MQ=255) aGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGTGTTCTGCGCGCCGGAACCAGCACCAg > 2:2345423/1‑99 (MQ=255) gAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGTGTTCTGCGCGCCGGAACCAGCACCAGt < 2:1927834/99‑1 (MQ=255) ccGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGTGTTCTGCGCGCCGGAACCAGCACCAGTACTGa > 1:1680662/1‑99 (MQ=255) ccGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGTGTTCTGCGCGCCGGAACCAGCACCAGTACTGa < 2:403730/99‑1 (MQ=255) ccGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGTGTTCTGCGCGCCGGAACCAGCACCAGTACTGa < 2:1535153/99‑1 (MQ=255) ccGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGTGTTCTGCGCGCCGGAACCAGCACCAGTACTGa > 1:2139009/1‑99 (MQ=255) cGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGTGTTCTGCGCGCCGGAACCAGCACCAGTACTGAt > 2:1939941/1‑99 (MQ=255) gAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGTGTTCTGCGCGCCGGAACCAGCACCAGTACTGATaa < 2:541647/99‑1 (MQ=255) cGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGTGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAACCa < 1:2177522/99‑1 (MQ=255) gaagaaGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGTGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAACCAg > 2:1967784/1‑99 (MQ=255) gaagaaGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGTGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAACCAg < 1:71822/99‑1 (MQ=255) gaagaaGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGTGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAACCAg < 2:559333/99‑1 (MQ=255) gaaggTTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGTGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAACCAGCgg > 2:2304444/1‑99 (MQ=255) aagaTTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGTGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAACCAGCGGc < 2:712941/99‑1 (MQ=255) aagaTTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGTGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAACCAGCGGc > 2:1309290/1‑99 (MQ=255) agaTTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGTGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAACCAGCGGCa > 1:667340/1‑99 (MQ=255) agaTTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGTGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAACCAGCGGCa > 1:748527/1‑99 (MQ=255) agaTTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGTGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAACCAGCGGCa > 2:1468739/1‑99 (MQ=255) ttGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGTGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAACCAGCGGCATaa < 2:110772/99‑1 (MQ=255) ttGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGTGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAACCAGCGGCATaa > 2:1358243/1‑99 (MQ=255) tGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGTGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAACCAGCGGCATAAc < 2:765898/99‑1 (MQ=255) tGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGTGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAACCAGCGGCATAAc > 1:1494830/1‑99 (MQ=255) ccAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGTGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAACCAGCGGCATAACGc > 1:1673830/1‑99 (MQ=255) ccAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGTGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAACCAGCGGCATAACGc > 1:2313423/1‑99 (MQ=255) gTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGTGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAACCAGCGGCATAACGCCGa > 2:1497580/1‑99 (MQ=255) tACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGTGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAACCAGCGGCATAACGCCGAg > 1:918135/1‑99 (MQ=255) tACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGTGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAACCAGCGGCATAACGCCGAg > 2:977977/1‑99 (MQ=255) tACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGTGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAACCAGCGGCATAACGCCGAg > 2:398420/1‑99 (MQ=255) ccGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGTGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAACCAGCGGCATAACGCCGAGGa < 2:292790/99‑1 (MQ=255) ccGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGTGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAACCAGCGGCATAACGCCGAGGa > 2:55554/1‑99 (MQ=255) cGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGTGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAACCAGCGGCATAACGCCGAGGAt < 2:2051988/99‑1 (MQ=255) gTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGTGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAACCAGCGGCATAACGCCGAGGATa < 1:1794242/99‑1 (MQ=255) cGGTCACCATCCCGCCCATTACACCGGTACCTACTGTGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAACCAGCGGCATAACGCCGAGGATAAACg < 2:1160522/99‑1 (MQ=255) ggTCACCATCCCGCCCATTACACCGGTACCTACTGTGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAACCAGCGGCATAACGCCGAGGATAAACGc < 2:518561/99‑1 (MQ=255) ggTCACCATCCCGCCCATTACACCGGTACCTACTGTGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAACCAGCGGCATAACGCCGAGGATAAACGc > 2:626933/1‑99 (MQ=255) gTCACCATCCCGCCCATTACACCGGTACCTACTGTGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAACCAGCGGCATAACGCCGAGGATAAACGcc > 2:1378925/1‑99 (MQ=255) gTCACCATCCCGCCCATTACACCGGTACCTACTGTGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAACCAGCGGCATAACGCCGAGGATAAACGcc > 1:1236108/1‑99 (MQ=255) aCCATCCCGCCCATTACACCGGTACCTACTGTGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAACCAGCGGCATAACGCCGAGGATAAACGCCAGc > 2:1873124/1‑99 (MQ=255) ccATCCCGCCCATTACACCGGTACCTACTGTGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAACCAGCGGCATAACGCCGAGGATAAACGCCAGCg > 1:832747/1‑99 (MQ=255) aTCCCGCCCATTACACCGGTACCTACTGTGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAACCAGCGGCATAACGCCGAGGATAAACGCCAGCGAg > 2:2103543/1‑99 (MQ=255) aTCCCGCCCATTACACCGGTACCTACTGTGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAACCAGCGGCATAACGCCGAGGATAAACGCCAGCGAg > 1:674431/1‑99 (MQ=255) tCCCGCCCATTACACCGGTACCTACTGTGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAACCAGCGGCATAACGCCGAGGATAAACGCCAGCGAgg > 2:1772853/1‑99 (MQ=255) tCCCGCCCATTACACCGGTACCTACTGTGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAACCAGCGGCATAACGCCGAGGATAAACGCCAGCGAgg > 2:1847093/1‑99 (MQ=255) tCCCGCCCATTACACCGGTACCTACTGTGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAACCAGCGGCATAACGCCGAGGATAAACGCCAGCGAgg < 1:1882398/99‑1 (MQ=255) cGCCCATTACACCGGTACCTACTGTGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAACCAGCGGCATAACGCCGAGGATAAACGCCAGCGAGGtca < 1:2006348/99‑1 (MQ=255) gCCCATTACACCGGTACCTTCTGTGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAACCAGCGGCATAACGCCGAGGATAAACGCCAGCGAGGtcat < 2:914059/99‑1 (MQ=255) gCCCATTACACCGGTACCTACTGTGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAACCAGCGGCATAACGCCGAGGATAAACGCCAGCGAGGtcat < 2:1687289/99‑1 (MQ=255) gCCCATTACACCGGTACCTACTGTGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAACCAGCGGCATAACGCCGAGGATAAACGCCAGCGAGGtcat < 2:1561022/99‑1 (MQ=255) cccATTACACCGGTACCTACTGTGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAACCAGCGGCATAACGCCGAGGATAAACGCCAGCGAGGtcatc > 2:1206884/1‑99 (MQ=255) ccATTACACCGGTACCTACTGTGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAACCAGCGGCATAACGCCGAGGATAAACGCCAGCGAGGtcatca > 1:240392/1‑99 (MQ=255) cATTACACCGGTACCTACTGTGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAACCAGCGGCATAACGCCGAGGATAAACGCCAGCGAGGTCATCAg < 2:884021/99‑1 (MQ=255) aTTACACCGGTACCTACTGTGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAACCAGCGGCATAACGCCGAGGATAAACGCCAGCGAGGTCATCAgg > 1:1068827/1‑99 (MQ=255) ttACACCGGTACCTACTGTGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAACCAGCGGCATAACGCCGAGGATAAACGCCAGCGAGGTCATCAGGa > 1:74845/1‑99 (MQ=255) tACACCGGTACCTACTGTGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAACCAGCGGCATAACGCCGAGGATAAACGCCAGCGAGGTCATCAGGAt < 1:2310805/99‑1 (MQ=255) acacCGGTACCTACTGTGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAACCAGCGGCATAACGCCGAGGATAAACGCCAGCGAGGTCATCAGGATc < 2:1426466/99‑1 (MQ=255) cacCGGTACCTACTGTGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAACCAGCGGCATAACGCCGAGGATAAACGCCAGCGAGGTCATCAGGATCg > 2:1615003/1‑99 (MQ=255) ccGGTACCTACTGTGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAACCAGCGGCATAACGCCGAGGATAAACGCCAGCGAGGTCATCAGGATCGGa < 2:24487/99‑1 (MQ=255) ccGGTACCTACTGTGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAACCAGCGGCATAACGCCGAGGATAAACGCCAGCGAGGTCATCAGGATCGGa < 1:2304614/99‑1 (MQ=255) cGGTACCTACTGTGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAACCAGCGGCATAACGCCGAGGATAAACGCCAGCGAGGTCATCAGGATCGGAc < 1:1637425/99‑1 (MQ=255) gTACCTACTGTGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAACCAGCGGCATAACGCCGAGGATAAACGCCAGCGAGGTCATCAGGATCGGACGt > 1:940990/1‑99 (MQ=255) ccTACTGTGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAACCAGCGGCATAACGCCGAGGATAAACGCCAGCGAGGTCATCAGGATCGGACGTaaa < 1:694442/99‑1 (MQ=255) cTACTGTGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAACCAGCGGCATAACGCCGAGGATAAACGCCAGCGAGGTCATCAGGATCGGACGTAAAc < 2:1873989/99‑1 (MQ=255) tACTGTGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAACCAGCGGCATAACGCCGAGGATAAACGCCAGCGAGGTCATCAGGATCGGACGTAAACg > 2:416062/1‑99 (MQ=255) | AAGCGGCGGCGAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAACCAGCGGCATAACGCCGAGGATAAACGCCAGCGAGGTCATCAGGATCGGACGTAAACG > NC_000913/481306‑481493 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |