Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,535,436 C→A 100% V145L (GTA→TTA)  envZ ← sensory histidine kinase EnvZ

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,535,4360CA100.0% 399.0 / NA 92V145L (GTA→TTA) envZsensory histidine kinase EnvZ
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (56/36);  total (56/36)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

GCCACGCCCCGCCTATCGCCAATAGCATAATCGCCAGCGTATAGCGGAACAGCGGAGAGAAATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGACGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCTGCG  >  NC_000913/3535340‑3535531
                                                                                                |                                                                                               
gCCACGCCCCGCCTATCGCCAATAGCATAATCGCCAGCGTATAGCGGAACAGCGGAGAGAAATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTAacc                                                                                               <  2:440520/99‑1 (MQ=255)
gCCACGCCCCGCCTATCGCCAATAGCATAATCGCCAGCGTATAGCGGAACAGCGGAGAGAAATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTAacc                                                                                               <  2:151197/99‑1 (MQ=255)
  cACGCCCCGCCTATCGCCAATAGCATAATCGCCAGCGTATAGCGGAACAGCGGAGAGAAATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTAACCAg                                                                                             >  1:984149/1‑99 (MQ=255)
   aCGCCCCGCCTATCGCCAATAGCATAATCGCCAGCGTATAGCGGAACAGCGGAGAGAAATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTAACCAGa                                                                                            <  2:281476/99‑1 (MQ=255)
   aCGCCCCGCCTATCGCCAATAGCATAATCGCCAGCGTATAGCGGAACAGCGGAGAGAAATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTAACCAGa                                                                                            <  2:279229/99‑1 (MQ=255)
       cccGCCTATCGCCAATAGCATAATCGCCAGCGTATAGCGGAACAGCGGAGAGAAATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTAACCAGATAtt                                                                                        >  1:1821716/1‑99 (MQ=255)
       cacGCCTATCGCCAATAGCATAATCGCCAGCGTATAGCGGAACAGCGGAGAGAAATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTAACCAGATAtt                                                                                        >  2:787488/3‑99 (MQ=255)
         cGCCTATCGCCAATAGCATAATCGCCAGCGTATAGCGGAACAGCGGAGAGAAATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTAACCAGATATTgg                                                                                      <  2:1206736/99‑1 (MQ=255)
         cGCCTATCGCCAATAGCATAATCGCCAGCGTATAGCGGAACAGCGGAGAGAAATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTAACCAGATATTgg                                                                                      <  1:759635/99‑1 (MQ=255)
             tATCGCCAATAGCATAATCGCCAGCGTATAGCGGAACAGCGGAGAGAAATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTAACCAGATATTGGGCGa                                                                                  >  2:1169608/1‑99 (MQ=255)
             tATCGCCAATAGCATAATCGCCAGCGTATAGCGGAACAGCGGAGAGAAATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTAACCAGATATTGGGCGa                                                                                  >  1:1193119/1‑99 (MQ=255)
             tATCGCCAATAGCATAATCGCCAGCGTATAGCGGAACAGCGGAGAGAAATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTAACCAGATATTGGGCGa                                                                                  <  2:844229/99‑1 (MQ=255)
              aTCGCCAATAGCATAATCGCCAGCGTATAGCGGAACAGCGGAGAGAAATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTAACCAGATATTGGGCGAc                                                                                 >  2:1994452/1‑99 (MQ=255)
                   cAATAGCATAATCGCCAGCGTATAGCGGAACAGCGGAGAGAAATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTAACCAGATATTGGGCGACAGCCa                                                                            >  2:1754581/1‑99 (MQ=255)
                   cAATAGCATAATCGCCAGCGTATAGCGGAACAGCGGAGAGAAATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTAACCAGATATTGGGCGACAGCCa                                                                            >  1:46214/1‑99 (MQ=255)
                    aaTAGCATAATCGCCAGCGTATAGCGGAACAGCGGAGAGAAATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTAACCAGATATTGGGCGACAGCCAg                                                                           >  1:1185233/1‑99 (MQ=255)
                     aTAGCATAATCGCCAGCGTATAGCGGAACAGCGGAGAGAAATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTAACCAGATATTGGGCGACAGCCAgg                                                                          <  1:562733/99‑1 (MQ=255)
                     aTAGCATAATCGCCAGCGTATAGCGGAACAGCGGAGAGAAATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTAACCAGATATTGGGCGACAGCCAgg                                                                          >  2:297858/1‑99 (MQ=255)
                      tAGCATAATCGCCAGCGTATAGCGGAACAGCGGAGAGAAATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTAACCAGATATTGGGCGACAGCCAGGt                                                                         <  2:1315723/99‑1 (MQ=255)
                      tAGCATAATCGCCAGCGTATAGCGGAACAGCGGAGAGAAATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTAACCAGATATTGGGCGACAGCCAGGt                                                                         >  1:1842534/1‑99 (MQ=255)
                      tAGCATAATCGCCAGCGTATAGCGGAACAGCGGAGAGAAATCGCCCTGATGAATCTCGGTCAGCGGCACGCGTAACCAGATATTGGGCGACAGCCAGGt                                                                         <  2:1035238/99‑1 (MQ=255)
                       aGCATAATCGCCAGCGTATAGCGGAACAGCGGAGAGAAATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTAACCAGATATTGGGCGACAGCCAGGtt                                                                        <  1:1405222/99‑1 (MQ=255)
                         cATAATCGCCAGCGTATAGCGGAACAGCGGAGAGAAATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTAACCAGATATTGGGCGACAGCCAGGtttt                                                                      <  2:2029410/99‑1 (MQ=255)
                         cATAATCGCCAGCGTATAGCGGAACAGCGGAGAGAAATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTAACCAGATATTGGGCGACAGCCAGGtttt                                                                      <  2:1916267/99‑1 (MQ=255)
                           tAATCGCCAGCGTATAGCGGAACAGCGGAGAGAAATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTAACCAGATATTGGGCGACAGCCAGGTTTTCa                                                                    >  1:1329008/1‑99 (MQ=255)
                           tAATCGCCAGCGTATAGCGGAACAGCGGAGAGAAATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTAACCAGATATTGGGCGACAGCCAGGTTTTCa                                                                    <  2:1330301/99‑1 (MQ=255)
                             aTCGCCAGCGTATAGCGGAACAGCGGAGAGAAATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTAACCAGATATTGGGCGACAGCCAGGTTTTCAGc                                                                  <  2:442413/99‑1 (MQ=255)
                                 ccAGCGTATAGCGGAACAGCGGAGAGAAATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTAACCAGATATTGGGCGACAGCCAGGTTTTCAGCCAga                                                              >  1:1558770/1‑99 (MQ=255)
                                 ccAGCGTATAGCGGAACAGCGGAGAGAAATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTAACCAGATATTGGGCGACAGCCAGGTTTTCAGCCAga                                                              <  1:1427130/99‑1 (MQ=255)
                                 ccAGCGTATAGCGGAACAGCGGAGAGAAATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTAACCAGATATTGGGCGACAGCCAGGTTTTCAGCCAga                                                              >  1:1865751/1‑99 (MQ=255)
                                  cAGCGTATAGCGGAACAGCGGAGAGAAATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTAACCAGATATTGGGCGACAGCCAGGTTTTCAGCCAgac                                                             >  2:1765160/1‑99 (MQ=255)
                                  cAGCGTATAGCGGAACAGCGGAGAGAAATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTAACCAGATATTGGGCGACAGCCAGGTTTTCAGCCAgac                                                             >  1:1121534/1‑99 (MQ=255)
                                  cAGCGTATAGCGGAACAGCGGAGAGAAATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTAACCAGATATTGGGCGACAGCCAGGTTTTCAGCCAgac                                                             <  2:1083164/99‑1 (MQ=255)
                                  cAGCGTATAGCGGAACAGCGGAGAGAAATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTAACCAGATATTGGGCGACAGCCAGGTTTTCAGCCAgac                                                             >  1:68194/1‑99 (MQ=255)
                                  cAGCGTATAGCGGAACAGCGGAGAGAAATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTAACCAGATATTGGGCGACAGCCAGGTTTTCAGCCAgac                                                             >  1:1711961/1‑99 (MQ=255)
                                   aGCGTATAGCGGAACAGCGGAGAGAAATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTAACCAGATATTGGGCGACAGCCATGTCTTCAGCCAgacg                                                            >  2:1420428/1‑99 (MQ=255)
                                       tataGCGGAACAGCGGAGAGAAATCGCCCTGATGAATTTTGGTCAGCGGCACGCGTAACCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGACGACAg                                                        >  1:331080/1‑99 (MQ=255)
                                       tataGCGGAACAGCGGAGAGAAATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTAACCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGACGACAg                                                        >  2:455611/1‑99 (MQ=255)
                                       tataGCGGAACAGCGGAGAGAAATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTAACCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGACGACAg                                                        >  1:179790/1‑99 (MQ=255)
                                         taGCGGAACAGCGGAGAGAAATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTAACCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGACGACAGGc                                                      <  2:719840/99‑1 (MQ=255)
                                           gCGGAACAGCGGAGAGAAATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTAACCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGACGACAGGCGa                                                    >  1:836036/1‑99 (MQ=255)
                                             ggAACAGCGGAGAGAAATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTAACCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGACGACAGGCGAAc                                                  >  1:699645/1‑99 (MQ=255)
                                              gAACAGCGGAGAGAAATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTAACCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGACGACAGGCGAACt                                                 <  1:143333/99‑1 (MQ=255)
                                              gAACAGCGGAGAGAAATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTAACCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGACGACAGGCGAACt                                                 >  2:175009/1‑99 (MQ=255)
                                              gAACAGCGGAGAGAAATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTAACCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGACGACAGGCGAACt                                                 >  2:1509866/1‑99 (MQ=255)
                                              gAACAGCGGAGAGAAATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTAACCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGACGACAGGCGAACt                                                 >  2:268632/1‑99 (MQ=255)
                                               aaCAGCGGAGAGAAATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTAACCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGACGACAGGCGAACtt                                                <  2:906810/99‑1 (MQ=255)
                                               aaCAGCGGAGAGAAATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTAACCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGACGACAGGCGAACtt                                                >  1:701651/1‑99 (MQ=255)
                                               aaCAGCGGAGAGAAATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTAACCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGACGACAGGCGAACtt                                                >  1:95068/1‑99 (MQ=255)
                                                aCAGCGGAGAGAAATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTAACCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGACGACAGGCGAACttt                                               <  1:120641/99‑1 (MQ=255)
                                                aCAGCGGAGAGAAATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTAACCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGACGACAGGCGAACttt                                               >  2:1674013/1‑99 (MQ=255)
                                                aCAGCGGAGAGAAATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTAACCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGACGACAGGCGAACttt                                               >  2:1938892/1‑99 (MQ=255)
                                                aCAGCGGAGAGAAATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTAACCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGACGACAGGCGAACttt                                               >  2:288980/1‑99 (MQ=255)
                                                 cAGCGGAGAGAAATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTAACCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGACGACAGGCGAACtttt                                              >  2:612000/1‑99 (MQ=255)
                                                  aGCGGAGAGAAATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTAACCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGACGACAGGCGAACTTttg                                             <  2:2295327/99‑1 (MQ=255)
                                                   gCGGAGAGAAATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTAACCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGACGACAGGCGAACTTttgt                                            >  1:1431543/1‑99 (MQ=255)
                                                   gCGGAGAGAAATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTAACCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGACGACAGGCGAACTTttgt                                            >  2:611213/1‑99 (MQ=255)
                                                   gCGGAGAGAAATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTAACCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGACGACAGGCGAACTTttgt                                            <  2:1069111/99‑1 (MQ=255)
                                                   gCGGAGAGAAATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTAACCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGACGACAGGCGAACTTttgt                                            >  1:780487/1‑99 (MQ=255)
                                                     ggAGAGAAATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTAACCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGACGACAGGCGAACTTttgttg                                          >  1:2334250/1‑99 (MQ=255)
                                                      gagagaAATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTAACCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGACGACAGGCGAACTTTTGTTGa                                         >  1:1476189/1‑99 (MQ=255)
                                                      gagagaAATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTAACCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGACGACAGGCGAACTTCTGTTGa                                         >  1:506485/1‑99 (MQ=255)
                                                        gagaAATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTAACCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGACGACAGGCGAACTTTTGTTGAcc                                       >  2:518265/1‑99 (MQ=255)
                                                         agaAATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTAACCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGACGACAGGCGAACTTTTGTTGACCt                                      >  2:1019007/1‑99 (MQ=255)
                                                          gaAATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTAACCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGACGACAGGCGAACTTTTGTTGACCTc                                     <  2:272134/99‑1 (MQ=255)
                                                           aaaTCGCCCTGATGAATTTCGGTCAGCGGCACGCGTAACCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGACGACAGGCGAACTTTTGTTGACCTCa                                    <  2:1425358/99‑1 (MQ=255)
                                                            aaTCGCCCTGATGAATTTCGGTCAGCGGCACGCGTAACCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGACGACAGGCGAACTTTTGTTGACCTCaa                                   >  1:96250/1‑99 (MQ=255)
                                                            aaTCGCCCTGATGAATTTCGGTCAGCGGCACGCGTAACCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGACGACAGGCGAACTTTTGTTGACCTCaa                                   >  1:405133/1‑99 (MQ=255)
                                                             aTCGCCCTGATGAATTTCGGTCAGCGGCACGCGTAACCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGACGACAGGCGAACTTTTGTTGACCTCAAc                                  >  2:657173/1‑99 (MQ=255)
                                                             aTCGCCCTGATGAATTTCGGTCAGCGGCACGCGTAACCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGACGACAGGCGAACTTTTGTTGACCTCAAc                                  >  1:396806/1‑99 (MQ=255)
                                                                gCCCTGATGAATTTCGGTCAGCGGCACGCGTAACCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGACGACAGGCGAACTTTTGTTGACCTCAAcgcg                               <  1:1046347/99‑1 (MQ=255)
                                                                 cccTGATGAATTTCGGTCAGCGGCACGCGTAACCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGACGACAGGCGAACTTTTGTTGACCTCAACgcgc                              >  1:1124941/1‑99 (MQ=255)
                                                                  ccTGATGAATTTCGGTCAGCGGCACGCGTAACCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGACGACAGGCGAACTTTTGTTGACCTCAACGCGCa                             <  1:1967031/99‑1 (MQ=255)
                                                                  ccTGATGAATTTCGGTCAGCGGCACGCGTAACCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGACGACAGGCGAACTTTTGTTGACCTCAACGCGCa                             <  2:1303706/99‑1 (MQ=255)
                                                                    tgatgaATTTCGGTCAGCGGCACGCGTAACCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGACGACAGGCGAACTTTTGTTGACCTCAACGCGCACt                           <  1:1966598/99‑1 (MQ=255)
                                                                    tgatgaATTTCGGTCAGCGGCACGCGTAACCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGACGACAGGCGAACTTTTGTTGACCTCAACGCGCACt                           >  2:1059380/1‑99 (MQ=255)
                                                                    tgatgaATTTCGGTCAGCGGCACGCGTAACCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGACGACAGGCGAACTTTTGTTGACCTCAACGCGCACt                           >  2:661284/1‑99 (MQ=255)
                                                                      atgaATTTCGGTCAGCGGCACGCGTAACCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGACGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTc                         <  1:855587/99‑1 (MQ=255)
                                                                       tgaATTTCGGTCAGCGGCACGCGTAACCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGACGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTcc                        >  2:1583009/1‑99 (MQ=255)
                                                                       tgaATTTCGGTCAGCGGCACGCGTAACCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGACGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTcc                        >  2:1518210/1‑99 (MQ=255)
                                                                        gaATTTCGGTCAGCGGCACGCGTAACCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGACGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCg                       <  2:131527/99‑1 (MQ=255)
                                                                        gaATTTCGGTCAGCGGCACGCGTAACCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGACGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCg                       <  2:67480/99‑1 (MQ=255)
                                                                                 tCAGCGGCACGCGTAACCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGACGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGgccgc              <  2:900430/99‑1 (MQ=255)
                                                                                  cAGCGGCACGCGTAACCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGACGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGgccgcc             <  1:1652535/99‑1 (MQ=255)
                                                                                   aGCGGCACGCGTAACCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGACGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGccc            >  1:523387/1‑99 (MQ=255)
                                                                                    gCGGCACGCGTAACCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGACGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCa           >  2:1650970/1‑99 (MQ=255)
                                                                                    gCGGCACGCGTAACCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGACGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCa           >  2:812658/1‑99 (MQ=255)
                                                                                      ggCACGCGTAACCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGACGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGt         <  2:1250193/99‑1 (MQ=255)
                                                                                      ggCACGCGTAACCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGACGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGt         <  2:1650059/99‑1 (MQ=255)
                                                                                         aCGCGTAACCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGACGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTtgc      <  2:1955643/99‑1 (MQ=255)
                                                                                          cgcgTAACCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGACGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTtgct     <  2:1214317/99‑1 (MQ=255)
                                                                                          cgcgTAACCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGACGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTtgct     >  2:326824/1‑99 (MQ=255)
                                                                                          cgcgTAACCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGACGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTtgct     >  2:2100683/1‑99 (MQ=255)
                                                                                             gTAACCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGACGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCTgcg  >  2:1553683/1‑99 (MQ=255)
                                                                                                |                                                                                               
GCCACGCCCCGCCTATCGCCAATAGCATAATCGCCAGCGTATAGCGGAACAGCGGAGAGAAATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGACGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCTGCG  >  NC_000913/3535340‑3535531

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑