Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,163,048 T→C 100% L58P (CTT→CCT)  baeS → sensory histidine kinase BaeS

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,163,0480TC100.0% 317.1 / NA 78L58P (CTT→CCT) baeSsensory histidine kinase BaeS
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (35/43);  total (35/43)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

TATGCACTGGGCGGTGCGTATCAGTTTTGAGCGTGGCTTTATTGATTACATCAAGCATGGTAATGAACAGCGATTACAACTGTTAAGTGATGCGCTTGGCGAGCAGTATGCGCAGCATGGCAACTGGCGCTTCCTGCGCAACAATGATCGCTTTGTCTTTCAGATCCTGCGTTCATTTGAACACGATAATT  >  NC_000913/2162953‑2163143
                                                                                               |                                                                                               
tATGCACTGGGCGGTGCGTATCAGTTTTGAGCGTGGCTTTATTGATTACATCAAGCATGGTAATGAACAGCGATTACAACTGTTAAGTGATGCGCCTgg                                                                                              >  1:2080801/1‑99 (MQ=255)
         ggCGGTGCGTATCAGTTTTGAGCGTGGCTTTATTGATTACATTAAGCATGGTAATGAACAGCGATTACAACTGTTAAGTGATGCGCCTGGCGAGCAGTa                                                                                     <  2:792436/99‑1 (MQ=255)
         ggCGGTGCGTATCAGTTTTGAGCGTGGCTTTATTGATTACATCAAGCATGGTAATGAACAGCGATTACAACTGTTAAGTGATGCGCCTGGCGAGCAGTa                                                                                     <  2:1975169/99‑1 (MQ=255)
         ggCGGTGCGTATCAGTTTTGAGCGTGGCTTTATTGATTACATCAAGCATGGTAATGAACAGCGATTACAACTGTTAAGTGATGCGCCTGGCGAGCAGTa                                                                                     <  2:630375/99‑1 (MQ=255)
         ggCGGTGCGTATCAGTTTTGAGCGTGGCTTTATTGATTACATCAAGCATGGTAATGAACAGCGATTACAACTGTTAAGTGATGCGCCTGGCGAGCAGTa                                                                                     <  1:1689895/99‑1 (MQ=255)
            ggTGCGTATCAGTTTTGAGCGTGGCTTTATTGATTACATCAAGCATGGTAATGAACAGCGATTACAACTGTTAAGTGATGCGCCTGGCGAGCAGTATgc                                                                                  <  1:1426616/99‑1 (MQ=255)
                  tATCAGTTTTGAGCGTGGCTTTATTGATTACATCAAGCATGGTAATGAACAGCGATTACAACTGTTAAGTGATGCGCCTGGCGAGCAGTATGCgcagca                                                                            >  1:1279827/1‑99 (MQ=255)
                  tATCAGTTTTGAGCGTGGCTTTATTGATTACATCAAGCATGGTAATGAACAGCGATTACAACTGTTAAGTGATGCGCCTGGCGAGCAGTATGCgcagca                                                                            <  2:630176/99‑1 (MQ=255)
                  tATCAGTTTTGAGCGTGGCTTTATTGATTACATCAAGCATGGTAATGAACAGCGATTACAACTGTTAAGTGATGCGCCTGGCGAGCAGTATGCgcagca                                                                            <  1:346518/99‑1 (MQ=255)
                   aTCAGTTTTGAGCGTGGCTTTATTGATTACATCAAGCATGGTAATGAACAGCGATTACAACTGTTAAGTGATGCGCCTGGCGAGCAGTATGCGCAGCAt                                                                           <  1:419080/99‑1 (MQ=255)
                     cAGTTTTGAGCGTGGCTTTATTGATTACATCAAGCATGGTAATGAACAGCGATTACAACTGTTAAGTGATGCGCCTGGCGAGCAGTATGCGCAGCATgg                                                                         <  1:1936887/99‑1 (MQ=255)
                      aGTTTTGAGCGTGGCTTTATTGATTACATCAAGCATGGTAATGAACAGCGATTACAACTGTTAAGTGATGCGCCTGGCGAGCAGTATGCGCAGCATGGc                                                                        <  1:510881/99‑1 (MQ=255)
                      aGTTTTGAGCGTGGCTTTATTGATTACATCAAGCATGGTAATGAACAGCGATTACAACTGTTAAGTGATGCGCCTGGCGAGCAGTATGCGCAGCATGGc                                                                        <  1:566579/99‑1 (MQ=255)
                       gTTTTGAGCGTGGCTTTATTGATTACATCAAGCATGGTAATGAACAGCGATTACAACTGTTAAGTGATGCGCCTGGCGAGCAGTATGCGCAGCATGGCa                                                                       >  2:2308824/1‑99 (MQ=255)
                         tttGAGCGTGGCTTTATTGATTACATCAAGCATGGTAATGAACAGCGATTACAACTGTTAAGTGATGCGCCTGGCGAGCAGTATGCGCAGCATGGCAAc                                                                     >  1:2316348/1‑99 (MQ=255)
                          ttGAGCGTGGCTTTATTGATTACATCAAGCATGGTAATGAACAGCGATTACAACTGTTAAGTGATGCGCCTGGCGAGCAGTATGCGCAGCATGGCAACt                                                                    <  1:1126097/99‑1 (MQ=255)
                          ttGAGCGTGGCTTTATTGATTACATCAAGCATGGTAATGAACAGCGATTACAACTGTTAAGTGATGCGCCTGGCGAGCAGTATGCGCAGCATGGCAACt                                                                    <  1:798997/99‑1 (MQ=255)
                            gAGCGTGGCTTTATTGATTACATCAAGCATGGTAATGAACAGCGATTACAACTGTTAAGTGATGCGCCTGGCGAGCAGTATGCGCAGCATGGCAACTgg                                                                  <  2:1213916/99‑1 (MQ=255)
                             aGCGTGGCTTTATTGATTACATCAAGCATGGTAATGAACAGCGATTACAACTGTTAAGTGATGCGCCTGGCGAGCAGTATGCGCAGCATGGCAACTGgc                                                                 >  2:191858/1‑99 (MQ=255)
                              gCGTGGCTTTATTGATTACATCAAGCATGGTAATGAACAGCGATTACAACTGTTAAGTGATGCGCCTGGCGAGCAGTATGCGCAGCATGGCAACTGgcg                                                                >  2:1605539/1‑99 (MQ=255)
                                   gCTTTATTGATTACATCAAGCATGGTAATGAACAGCGATTACAACTGTTAAGTGATGCGCCTGGCGAGCAGTATGCGCAGCATGGCAACTGGCGCTTcc                                                           <  2:1081347/99‑1 (MQ=255)
                                   gCTTTATTGATTACATCAAGCATGGTAATGAACAGCGATTACAACTGTTAAGTGATGCGCCTGGCGAGCAGTATGCGCAGCATGGCAACTGGCGCTTcc                                                           <  1:988939/99‑1 (MQ=255)
                                    cTTTATTGATTACATCAAGCATGGTAATGAACAGCGATTACAACTGTTAAGTGATGCGCCTGGCGAGCAGTATGCGCAGCATGGCAACTGGCGCTTCCt                                                          <  2:1447947/99‑1 (MQ=255)
                                       tATTGATTACATCAAGCATGGTAATGAACAGCGATTACAACTGTTAAGTGATGCGCCTGGCGAGCAGTATGCGCAGCATGGCAACTGGCGCTTCCTgcg                                                       >  1:1910633/1‑99 (MQ=255)
                                            aTTACATCAAGCATGGTAATGAACAGCGATTACAACTGTTAAGTGATGCGCCTGGCGAGCAGTATGCGCAGCATGGCAACTGGCGCTTCCTGCGcaaca                                                  >  2:300359/1‑99 (MQ=255)
                                            aTTACATCAAGCATGGTAATGAACAGCGATTACAACTGTTAAGTGATGCGCCTGGCGAGCAGTATGCGCAGCATGGCAACTGGCGCTTCCTGCGcaaca                                                  >  2:524794/1‑99 (MQ=255)
                                            aTTACATCAAGCATGGTAATGAACAGCGATTACAACTGTTAAGTGATGCGCCTGGCGAGCAGTATGCGCAGCATGGCAACTGGCGCTTCCTGCGcaaca                                                  >  1:719522/1‑99 (MQ=255)
                                             ttACATCAAGCATGGTAATGAACAGCGATTACAACTGTTAAGTGATGCGCCTGGCGAGCAGTATGCGCAGCATGGCAACTGGCGCTTCCTGCGcaacaa                                                 >  2:1966007/1‑99 (MQ=255)
                                             ttACATCAAGCATGGTAATGAACAGCGATTACAACTGTTAAGTGATGCGCCTGGCGAGCAGTATGCGCAGCATGGCAACTGGCGCTTCCTGCGcaacaa                                                 <  2:1918250/99‑1 (MQ=255)
                                             ttACATCAAGCATGGTAATGAACAGCGATTACAACTGTTAAGTGATGCGCCTGGCGAGCAGTATGCGCAGCATGGCAACTGGCGCTTCCTGCGcaacaa                                                 <  1:891580/99‑1 (MQ=255)
                                             ttACATCAAGCATGGTAATGAACAGCGATTACAACTGTTAAGTGATGCGCCTGGCGAGCAGTATGCGCAGCATGGCAACTGGCGCTTCCTGCGcaacaa                                                 <  2:2081447/99‑1 (MQ=255)
                                              tACATCAAGCATGGTAATGAACAGCGATTACAACTGTTAAGTGATGCGCCTGGCGAGCAGTATGCGCAGCATGGCAACTGGCGCTTCCTGCGCAACAAt                                                <  1:31841/99‑1 (MQ=255)
                                                cATCAAGCATGGTAATGAACAGCGATTACAACTGTTAAGTGATGCGCCTGGCGAGCAGTATGCGCAGCATGGCAACTGGCGCTTCCTGCGCAACAATGa                                              <  2:1883266/99‑1 (MQ=255)
                                                cATCAAGCATGGTAATGAACAGCGATTACAACTGTTAAGTGATGCGCCTGGCGAGCAGTATGCGCAGCATGGCAACTGGCGCTTCCTGCGCAACAATGa                                              >  1:84684/1‑99 (MQ=255)
                                                cATCAAGCATGGTAATGAACAGCGATTACAACTGTTAAGTGATGCGCCTGGCGAGCAGTATGCGCAGCATGGCAACTGGCGCTTCCTGCGCAACAATGa                                              >  1:1541773/1‑99 (MQ=255)
                                                  tCAAGCATGGTAATGAACAGCGATTACAACTGTTAAGTGATGCGCCTGGCGAGCAGTATGCGCAGCATGGCAACTGGCGCTTCCTGCGCAACAATGATc                                            >  2:1447268/1‑99 (MQ=255)
                                                   cAAGCATGGTAATGAACAGCGATTACAACTGTTAAGTGATGCGCCTGGCGAGCAGTATGCGCAGCATGGCAACTGGCGCTTCCTGCGCAACAATGATCg                                           <  1:28252/99‑1 (MQ=255)
                                                    aaGCATGGTAATGAACAGCGATTACAACTGTTAAGTGATGCGCCTGGCGAGCAGTATGCGCAGCATGGCAACTGGCGCTTCCTGCGCAACAATGATCGc                                          >  1:191125/1‑99 (MQ=255)
                                                     aGCATGGTAATGAACAGCGATTACAACTGTTAAGTGATGCGCCTGGCGAGCAGTATGCGCAGCATGGCAACTGGCGCTTCCTGCGCAACAATGATCGCt                                         <  2:410135/99‑1 (MQ=255)
                                                      gCATGGTAATGAACAGCGATTACAACTGTTAAGTGATGCGCCTGGCGAGCAGTATGCGCAGCATGGCAACTGGCGCTTCCTGCGCAACAATGATCGCtt                                        <  2:953318/99‑1 (MQ=255)
                                                        aTGGTAATGAACAGCGATTACAACTGTTAAGTGATGCGCCTGGCGAGCAGTATGCGCAGCATGGCAACTGGCGCTTCCTGCGCAACAATGATCGCTTTg                                      <  2:1280206/99‑1 (MQ=255)
                                                        aTGGTAATGAACAGCGATTACAACTGTTAAGTGATGCGCCTGGCGAGCAGTATGCGCAGCATGGCAACTGGCGCTTCCTGCGCAACAATGATCGCTTTg                                      >  2:1685996/1‑99 (MQ=255)
                                                          ggTAATGAACAGCGATTACAACTGTTAAGTGATGCGCCTGGCGAGCAGTATGCGCAGCATGGCAACTGGCGCTTCCTGCGCAACAATGATCGCTTTGTc                                    <  1:168023/99‑1 (MQ=255)
                                                          ggTAATGAACAGCGATTACAACTGTTAAGTGATGCGCCTGGCGAGCAGTATGCGCAGCATGGCAACTGGCGCTTCCTGCGCAACAATGATCGCTTTGTc                                    <  1:1989461/99‑1 (MQ=255)
                                                           gTAATGAACAGCGATTACAACTGTTAAGTGATGCGCCTGGCGAGCAGTATGCGCAGCATGGCAACTGGCGCTTCCTGCGCAACAATGATCGCTTTGTCt                                   >  1:1359112/1‑99 (MQ=255)
                                                                gAACAGCGATTACAACTGTTAAGTGATGCGCCTGGCGAGCAGTATGCGCAGCATGGCAACTGGCGCTTCCTGCGCAACAATGATCGCTTTGTCTTTCAg                              <  1:1937637/99‑1 (MQ=255)
                                                                 aaCAGCGATTACAACTGTTAAGTGATGCGCCTGGCGAGCAGTATGCGCAGCATGGCAACTGGCGCTTCCTGCGCAACAATGATCGCTTTGTCTTTCAGa                             >  1:1236677/1‑99 (MQ=255)
                                                                   cAGCGATTACAACTGTTAAGTGATGCGCCTGGCGAGCAGTATGCGCAGCATGGCAACTGGCGCTTCCTGCGCAACAATGATCGCTTTGTCTTTCAGATc                           <  2:1126601/99‑1 (MQ=255)
                                                                   cAGCGATTACAACTGTTAAGTGATGCGCCTGGCGAGCAGTATGCGCAGCATGGCAACTGGCGCTTCCTGCGCAACAATGATCGCTTTGTCTTTCAGATc                           <  2:1496603/99‑1 (MQ=255)
                                                                    aGCGATTACAACTGTTAAGTGATGCGCCTGGCGAGCAGTATGCGCAGCATGGCAACTGGCGCTTCCTGCGCAACAATGATCGCTTTGTCTTTCAGATcc                          >  1:951169/1‑99 (MQ=255)
                                                                    aGCGATTACAACTGTTAAGTGATGCGCCTGGCGAGCAGTATGCGCAGCATGGCAACTGGCGCTTCCTGCGCAACAATGATCGCTTTGTCTTTCAGATcc                          >  2:622147/1‑99 (MQ=255)
                                                                    aGCGATTACAACTGTTAAGTGATGCGCCTGGCGAGCAGTATGCGCAGCATGGCAACTGGCGCTTCCTGCGCAACAATGATCGCTTTGTCTTTCAGATcc                          >  1:197358/1‑99 (MQ=255)
                                                                    aGCGATTACAACTGTTAAGTGATCCGCCTGGCGAGCAGTATGCGCAGCATGGCAACTGGCGCTTCCTGCGCAACAATGATCGCTTTGTCTTTCAGATcc                          <  2:770660/99‑1 (MQ=255)
                                                                     gCGATTACAACTGTTAAGTGATGCGCCTGGCGAGCAGTATGCGCAGCATGGCAACTGGCGCTTCCTGCGCAACAATGATCGCTTTGTCTTTCAGATCCt                         >  1:1200349/1‑99 (MQ=255)
                                                                     gCGATTACAACTGTTAAGTGATGCGCCTGGCGAGCAGTATGCGCAGCATGGCAACTGGCGCTTCCTGCGCAACAATGATCGCTTTGTCTTTCAGATCCt                         >  1:1480685/1‑99 (MQ=255)
                                                                     gCGATTACAACTGTTAAGTGATGCGCCTGGCGAGCAGTATGCGCAGCATGGCAACTGGCGCTTCCTGCGCAACAATGATCGCTTTGTCTTTCAGATCCt                         >  2:1918507/1‑99 (MQ=255)
                                                                      cGATTACAACTGTTAAGTGATGCGCCTGGCGAGCAGTATGCGCAGCATGGCAACTGGCGCTTCCTGCGCAACAATGATCGCTTTGTCTTTCAGATCCTg                        <  2:321457/99‑1 (MQ=255)
                                                                       gATTACAACTGTTAAGTGATGCGCCTGGCGAGCAGTATGCGCAGCATGGCAACTGGCGCTTCCTGCGCAACAATGATCGCTTTGTCTTTCAGATCCTGc                       <  1:2312036/99‑1 (MQ=255)
                                                                        aTTACAACTGTTAAGTGATGCGCCTGGCGAGCAGTATGCGCAGCATGGCAACTGGCGCTTCCTGCGCAACAATGATCGCTTTGTCTTTCAGATCCTGCg                      >  2:1175563/1‑99 (MQ=255)
                                                                        aTTACAACTGTTAAGTGATGCGCCTGGCGAGCAGTATGCGCAGCATGGCAACTGGCGCTTCCTGCGCAACAATGATCGCTTTGTCTTTCAGATCCTGCg                      >  1:525252/1‑99 (MQ=255)
                                                                         ttACAACTGTTAAGTGATGCGCCTGGCGAGCAGTATGCGCAGCATGGCAACTGGCGCTTCCTGCGCAACAATGATCGCTTTGTCTTTCAGATCCTGCGt                     <  2:1623729/99‑1 (MQ=255)
                                                                            cAACTGTTAAGTGATGCGCCTGGCGAGCAGTATGCGCAGCATGGCAACTGGCGCTTCCTGCGCAACAATGATCGCTTTGTCTTTCAGATCCTGCGTTCa                  >  1:188111/1‑99 (MQ=255)
                                                                            cAACTGTTAAGTGATGCGCCTGGCGAGCAGTATGCGCAGCATGGCAACTGGCGCTTCCTGCGCAACAATGATCGCTTTGTCTTTCAGATCCTGCGTTCa                  <  1:412417/99‑1 (MQ=255)
                                                                             aaCTGTTAAGTGATGCGCCTGGCGAGCAGTATGCGCAGCATGGCAACTGGCGCTTCCTGCGCAACAATGATCGCTTTGTCTTTCAGATCCTGCGTTCAt                 <  1:488646/99‑1 (MQ=255)
                                                                              aCTGTTAAGTGATGCGCCTGGCGAGCAGTATGCGCAGCATGGCAACTGGCGCTTCCTGCGCAACAATGATCGCTTTGTCTTTCAGATCCTGCGTTCAtt                >  2:2171724/1‑99 (MQ=255)
                                                                              aCTGTTAAGTGATGCGCCTGGCGAGCAGTATGCGCAGCATGGCAACTGGCGCTTCCTGCGCAACAATGATCGCTTTGTCTTTCAGATCCTGCGTTCAtt                >  2:240467/1‑99 (MQ=255)
                                                                               cTGTTAAGTGATGCGCCTGGCGAGCAGTATGCGCAGCATGGCAACTGGCGCTTCCTGCGCAACAATGATCGCTTTGTCTTTCAGATCCTGCGTTCAttt               <  2:1013863/99‑1 (MQ=255)
                                                                                tGTTAAGTGATGCGCCTGGCGAGCAGTATGCGCAGCATGGCAACTGGCGCTTCCTGCGCAACAATGATCGCTTTGTCTTTCAGATCCTGCGTTCATTTg              <  1:605183/99‑1 (MQ=255)
                                                                                  ttAAGTGATGCGCCTGGCGAGCAGTATGCGCAGCATGGCAACTGGCGCTTCCTGCGCAACAATGATCGCTTTGTCTTTCAGATCCTGCGTTCATTTGaa            >  2:26872/1‑99 (MQ=255)
                                                                                  ttAAGTGATGCGCCTGGCGAGCAGTATGCGCAGCATGGCAACTGGCGCTTCCTGCGCAACAATGATCGCTTTGTCTTTCAGATCCTGCGTTCATTTGaa            >  2:1450629/1‑99 (MQ=255)
                                                                                    aaGTGATGCGCCTGGCGAGCAGTATGCGCAGCATGGCAACTGGCGCTTCCTGCGCAACAATGATCGCTTTGTCTTTCAGATCCTGCGTTCATTTGAaca          >  2:2089398/1‑99 (MQ=255)
                                                                                       tGATGCGCCTGGCGAGCAGTATGCGCAGCATGGCAACTGGCGCTTCCTGCGCAACAATGATCGCTTTGTCTTTCAGATCCTGCGTTCATTTGAACACGa       <  2:1184381/99‑1 (MQ=255)
                                                                                        gATGCGCCTGGCGAGCAGTATGCGCAGCATGGCAACTGGCGCTTCCTGCGCAACAATGATCGCTTTGTCTTTCAGATCCTGCGTTCATTTGAACACGAt      >  1:1306466/1‑99 (MQ=255)
                                                                                         aTGCGCCTGGCGAGCAGTATGCGCAGCATGGCAACTGGCGCTTCCTGCGCAACAATGATCGCTTTGTCTTTCAGATCCTGCGTTCATTTGAACACGATa     >  1:681286/1‑99 (MQ=255)
                                                                                          tGCGCCTGGCGAGCAGTATGCGCAGCATGGCAACTGGCGCTTCCTGCGCAACAATGATCGCTTTGTCTTTCAGATCCTGCGTTCATTTGAACACGATaa    <  2:334424/99‑1 (MQ=255)
                                                                                           gcgcCTGGCGAGCAGTATGCGCAGCATGGCAACTGGCGCTTCCTGCGCAACAATGATCGCTTTGTCTTTCAGATCCTGCGTTCATTTGAACACGATAAt   <  2:1750868/99‑1 (MQ=255)
                                                                                           gcgcCTGGCGAGCAGTATGCGCAGCATGGCAACTGGCGCTTCCTGCGCAACAATGATCGCTTTGTCTTTCAGATCCTGCGTTCATTTGAACACGATAAt   <  2:2159315/99‑1 (MQ=255)
                                                                                            cgcCTGGCGAGCAGTATGCGCAGCATGGCAACTGGCGCTTCCTGCGCAACAATGATCGCTTTGTCTTTCAGATCCTGCGTTCATTTGAACACGATAAtt  >  2:742143/1‑99 (MQ=255)
                                                                                               |                                                                                               
TATGCACTGGGCGGTGCGTATCAGTTTTGAGCGTGGCTTTATTGATTACATCAAGCATGGTAATGAACAGCGATTACAACTGTTAAGTGATGCGCTTGGCGAGCAGTATGCGCAGCATGGCAACTGGCGCTTCCTGCGCAACAATGATCGCTTTGTCTTTCAGATCCTGCGTTCATTTGAACACGATAATT  >  NC_000913/2162953‑2163143

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑