| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 3,535,315 | G→A | 23.3% | P185L (CCG→CTG) | envZ ← | sensory histidine kinase EnvZ |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,535,315 | 0 | G | A | 23.3% | 126.7 / 41.5 | 60 | P185L (CCG→CTG) | envZ | sensory histidine kinase EnvZ |
| Reads supporting (aligned to +/- strand): ref base G (26/20); new base A (7/7); total (33/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.63e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.60e-01 | |||||||||||
GGTAACGGAACGCACCTCCGAAGCGCCATACTCACGCAGCGGCGGCGGAATAATCCCTTTACCAACCTGCAAGGCTGCGTGTTCGAGATCGACCAACGGTCGGTTCTGGATACGAATAAACAGCCACGCCCCGCCTATCGCCAATAGCATAATCGCCAGCGTATAGCGGAACAGCGGAGAGAAATCGCCCTGA > NC_000913/3535218‑3535410 | cggcgtgcgcaagtcgtgacttacccccgccatcagcagcgGCGGCGGAATAATCCCTTTACCAACCTGCAAGGCTGCGTGTTCGAGATCGACCAAcgg < 2:1377999‑M2/58‑1 (MQ=255) ggcgtgcgcaagtcgtgacttacccccgccatcagcagcgGCGGCGGAATAATCCCTTTACCAACCTGCAAGGCTGCGTGTTCGAGATCGACCAAcggt < 2:626538‑M2/59‑1 (MQ=255) gTAACGGAACGCACCTCCGAAGCGCCATACTCACGCAGCGGCGGCGGAATAATCCCTTTACCAACCTGCAAGGCTGCGTGTTCGAGATCGACCAAcagt < 2:1345760/99‑1 (MQ=255) aaCGGAACGCACCTCCGAAGCGCCATACTCACGCAGCGGCGGCGGAATAATCCCTTTACCAACCTGCAAGGCTGCGTGTTCGAGATCGACCAAcagtcg > 1:618307/1‑99 (MQ=255) aCGGAACGCACCTCCGAAGCGCCATACTCACGCAGCGGCGGCGGAATAATCCCTTTACCAACCTGCAAGGCTGCGTGTTCGAGATCGACCAAcggtcgg > 2:25172/1‑99 (MQ=255) aCGGAACGCACCTCCGAAGCGCCATACTCACGCAGCGGCGGCGGAATAATCCCTTTACCAACCTGCAAGGCTGCGTGTTCGAGATCGACCAAcagtcgg > 2:1084864/1‑99 (MQ=255) tgcgcaagtcgtgacttacccccgccatcagcagcgGCGGCGGAATAATCCCTTTACCAACCTGCAAGGCTGCGTGTTCGAGATCGACCAAcggtcggt > 1:2188521‑M2/37‑99 (MQ=255) gAACGCACCTCCGAAGCGCCATACTCACGCAGCGGCGGCGGAATAATCCCTTTACCAACCTGCAAGGCTGCGTGTTCGAGATCGACCAACAGTCGGTTc > 1:1903697/1‑99 (MQ=255) gAACGCACCTCCGAAGCGCCATACTCACGCAGCGGCGGCGGAATAATCCCTTTACCAACCTGCAAGGCTGCGTGTTCGAGATCGACCAACAGTCGGTTc > 2:1470257/1‑99 (MQ=255) caagtcgtgacttacccccgccatcagcagcgGCGGCGGAATAATCCCTTTACCAACCTGCAAGGCTGCGTGTTCGAGATCGACCAACGGTCGGTTCTg > 2:1058668‑M2/33‑99 (MQ=255) aCGCACCTCCGAAGCGCCATACTCACGCAGCGGCGGCGGAATAATCCCTTTACCAACCTGCAAGGCTGCGTGTTCGAGATCGACCAACGGTCGGTTCTg > 2:952478/1‑99 (MQ=255) aagtcgtgacttacccccgccatcagcagcgGCGGCGGAATAATCCCTTTACCAACCTGCAAGGCTGCGTGTTCGAGATCGACCAACGGTCGGTTCTgg > 1:1094054‑M2/32‑99 (MQ=255) aCCTCCGAAGCGCCATACTCACGCAGCGGCGGCGGAATAATCCCTTTACCAACCTGCAAGGCTGCGTGTTCGAGATCGACCAACAGTCGGTTCTGGATa > 1:2263633/1‑99 (MQ=255) gtgacttacccccgccatcagcagcgGCGGCGGAATAATCCCTTTACCAACCTGCAAGGCTGCGTGTTCGAGATCGACCAACGGTCGGTTCTGGATACg > 1:1648369‑M2/27‑99 (MQ=255) tgacttacccccgccatcagcagcgGCGGCGGAATAATCCCTTTACCAACCTGCAAGGCTGCGTGTTCGAGATCGACCAACGGTCGGTTCTGGATACGa > 1:1671600‑M2/26‑99 (MQ=255) tgacttacccccgccatcagcagcgGCGGCGGAATAATCCCTTTACCAACCTGCAAGGCTGCGTGTTCGAGATCGACCAACGGTCGGTTCTGGATACGa > 1:766336‑M2/26‑99 (MQ=255) ccGAAGCGCCATACTCACGCAGCGGCGGCGGAATAATCCCTTTACCAACCTGCAAGGCTGCGTGTTCGAGATCGACCAACGGTCGGTTCTGGATACGaa < 1:1323015/99‑1 (MQ=255) ccGAAGCGCCATACTCACGCAGCGGCGGCGGAATAATCCCTTTACCAACCTGCAAGGCTGCGTGTTCGAGATCGACCAACAGTCGGTTCTGGATACGaa < 1:1640638/99‑1 (MQ=255) aGCGCCATACTCACGCAGCGGCGGCGGAATAATCCCTTTACCAACCTGCAAGGCTGCGTGTTCGAGATCGACCAACGGTCGGTTCTGGATACGAATaaa < 2:2222931/99‑1 (MQ=255) gcCATACTCACGCAGCGGCGGCGGAATAATCCCTTTACCAACCTGCAAGGCTGCGTGTTCGAGATCGACCAACGGTCGGTTCTGGATACGAATAAACAg < 1:712712/99‑1 (MQ=255) ccATACTCACGCAGCGGCGGCGGAATAATCCCTTTACCAACCTGCAAGGCTGCGTGTTCGAGATCGACCAACGGTCGGTTCTGGATACGAATAAACAGc < 1:863318/99‑1 (MQ=255) ccATACTCACGCAGCGGCGGCGGAATAATCCCTTTACCAACCTGCAAGGCTGCGTGTTCGAGATCGACCAACAGTCGGTTCTGGATACGAATAAACAGc < 2:1580210/99‑1 (MQ=255) ccgccatcagcagcgGCGGCGGAATAATCCCTTTACCAACCTGCAAGGCTGCGTGTTCGAGATCGACCAACGGTCGGTTCTGGATACGAATAAACAGcc > 1:1514266‑M2/16‑99 (MQ=255) cATACTCACGCAGCGGCGGCGGAATAATCCCTTTACCAACCTGCAAGGCTGCGTGTTCGAGATCGACCAACGGTCGGTTCTGGATACGAATAAACAGcc > 2:392341/1‑99 (MQ=255) cgccatcagcagcgGCGGCGGAATAATCCCTTTACCAACCTGCAAGGCTGCGTGTTCGAGATCGACCAACGGTCGGTTCTGGATACGAATAAACAGCCa < 2:1016207‑M2/85‑1 (MQ=255) aTACTCACGCAGCGGCGGCGGAATAATCCCTTTACCAACCTGCAAGGCTGCGTGTTCGAGATCGACCAACGGTCGGTTCTGGATACGAATAAACAGCCa < 1:1472021/99‑1 (MQ=255) cagcagcgGCGGCGGAATAATCCCTTTACCAACCTGCAAGGCTGCGTGTTCGAGATCGACCAACGGTCGGTTCTGGATACGAATAAACAGCCACGcccc < 1:527546‑M2/91‑1 (MQ=255) aCGCAGCGGCGGCGGAATAATCCCTTTACCAACCTGCAAGGCTGCGTGTTCGAGATCGACCAACGGTCGGTTCTGGATACGAATAAACAGCCACGcccc < 1:832541/99‑1 (MQ=255) gcagcgGCGGCGGAATAATCCCTTTACCAACCTGCAAGGCTGCGTGTTCGAGATCGACCAACGGTCGGTTCTGGATACGAATAAACAGCCACGCCCCGc > 1:804058‑M2/7‑99 (MQ=255) gcagcgGCGGCGGAATAATCCCTTTACCAACCTGCAAGGCTGCGTGTTCGAGATCGACCAACGGTCGGTTCTGGATACGAATAAACAGCCACGCCCCGc > 1:1459642‑M2/7‑99 (MQ=255) agcgGCGGCGGAATAATCCCTTTACCAACCTGCAAGGCTGCGTGTTCGAGATCGACCAACGGTCGGTTCTGGATACGAATAAACAGCCACGCCCCGCCt < 2:1973261‑M2/95‑1 (MQ=255) cgGCGGCGGAATAATCCCTTTACCAACCTGCAAGGCTGCGTGTTCGAGATCGACCAACGGTCGGTTCTGGATACGAATAAACAGCCACGCCCCGCCTAt < 1:309505‑M2/97‑1 (MQ=255) cgGCGGCGGAATAATCCCTTTACCAACCTGCAAGGCTGCGTGTTCGAGATCGACCAACAGTCGGTTCTGGATACGAATAAACAGCCACGCCCCGCCTAt < 1:1567700‑M2/97‑1 (MQ=255) gGCGGCGGAATAATCCCTTTACCAACCTGCAAGGCTGCGTGTTCGAGATCGACCAACGGTCGGTTCTGGATACGAATAAACAGCCACGCCCCGCCTATc < 1:2306243‑M2/98‑1 (MQ=255) cggcggAATAATCCCTTTACCAACCTGCAAGGCTGCGTGTTCGAGATCGACCAACAGTCGGTTCTGGATACGAATAAACAGCCACGCCCCGCCTATCGc < 2:714794/99‑1 (MQ=255) ggcggAATAATCCCTTTACCAACCTGCAAGGCTGCGTGTTCGAGATCGACCAACGGTCGGTTCTGGATACGAATAAACAGCCACGCCCCGCCTATCGcc > 1:1888417/1‑99 (MQ=255) gcggAATAATCCCTTTACCAACCTGCAAGGCTGCGTGTTCGAGATCGACCAACGGTCGGTTCTGGATACGAATAAACAGCCACGCCCCGCCTATCGCCa > 1:1009026/1‑99 (MQ=255) ggAATAATCCCTTTACCAACCTGCAAGGCTGCGTGTTCGAGATCGACCAACGGTCGGTTCTGGATACGAATAAACAGCCACGCCCCGCCTATCGCCAAt > 2:1692890/1‑99 (MQ=255) ggAATAATCCCTTTACCAACCTGCAAGGCTGCGTGTTCGAGATCGACCAACAGTCGGTTCTGGATACGAATAAACAGCCACGCCCCGCCTATCGCCAAt > 1:1994168/1‑99 (MQ=255) ggAATAATCCCTTTACCAACCTGCAAGGCTGCGTGTTCGAGATCGACCAACAGTCGGTTCTGGATACGAATAAACAGCCACGCCCCGCCTATCGCCAAt > 1:1881012/1‑99 (MQ=255) gAATAATCCCTTTACCAACCTGCAAGGCTGCGTGTTCGAGATCGACCAACGGTCGGTTCTGGATACGAATAAACAGCCACGCCCCGCCTATCGCCAATa > 2:273305/1‑99 (MQ=255) aataatCCCTTTACCAACCTGCAAGGCTGCGTGTTCGAGATCGACCAACGGTCGGTTCTGGATACGAATAAACAGCCACGCCCCGCCTATCGCCAATAg < 1:1864520/99‑1 (MQ=255) aataatCCCTTTACCAACCTGCAAGGCTGCGTGTTCGAGATCGACCAACAGTCGGTTCTGGATACGAATAAACAGCCACGCCCCGCCTATCGCCAATAg < 1:1472310/99‑1 (MQ=255) ataatCCCTTTACCAACCTGCAAGGCTGCGTGTTCGAGATCGACCAACGGTCGGTTCTGGATACGAATAAACAGCCACGCCCCGCCTATCGCCAATAGc > 1:1172035/1‑99 (MQ=255) cccTTTACCAACCTGCAAGGCTGCGTGTTCGAGATCGACCAACGGTCGGTTCTGGATACGAATAAACAGCCACGCCCCGCCTATCGCCAATAGCATAAt < 1:1066642/99‑1 (MQ=255) cccTTTACCAACCTGCAAGGCTGCGTGTTCGAGATCGACCAACGGTCGGTTCTGGATACGAATAAACAGCCACGCCCCGCCTATCGCCAATAGCATAAt < 1:1827442/99‑1 (MQ=255) ccTTTACCAACCTGCAAGGCTGCGTGTTCGAGATCGACCAACGGTCGGTTCTGGATACGAATAAACAGCCACGCCCCGCCTATCGCCAATAGCATAATc > 2:2112436/1‑99 (MQ=255) tACCAACCTGCAAGGCTGCGTGTTCGAGATCGACCAACGGTCGGTTCTGGATACGAATAAACAGCCACGCCCCGCCTATCGCCAATAGCATAATCGCCa < 2:1308732/99‑1 (MQ=255) aCCTGCAAGGCTGCGTGTTCGAGATCGACCAACGGTCGGTTCTGGATACGAATAAACAGCCACGCCCCGCCTATCGCCAATAGCATAATCGCCAGCGta > 1:1213903/1‑99 (MQ=255) aCCTGCAAGGCTGCGTGTTCGAGATCGACCAACAGTCGGTTCTGGATACGAATAAACAGCCACGCCCCGCCTATCGCCAATAGCATAATCGCCAGCGta < 2:1768875/99‑1 (MQ=255) aaGGCTGCGTGTTCGAGATCGACCAACAGTCGGTTCTGGATACGAATAAACAGCCACGCCCCGCCTATCGCCAATAGCATAATCGCCAGCGTATAGCgg > 2:1016936/1‑99 (MQ=255) aGGCTGCGTGTTCGAGATCGACCAACGGTCGGTTCTGGATACGAATAAACAGCCACGCCCCGCCTATCGCCAATAGCATAATCGCCAGCGTATAGCGGa > 2:90826/1‑99 (MQ=255) aGGCTGCGTGTTCGAGATCGACCAACGGTCGGTTCTGGATACGAATAAACAGCCACGCCCCGCCTATCGCCAATAGCATAATCGCCAGCGTATAGCGGa > 1:1506392/1‑99 (MQ=255) ggCTGCGTGTTCGAGATCGACCAACGGTCGGTTCTGGATACGAATAAACAGCCACGCCCCGCCTATCGCCAATAGCATAATCGCCAGCGTATAGCGGaa > 2:1039425/1‑99 (MQ=255) gCGTGTTCGAGATCGACCAACGGTCGGTTCTGGATACGAATAAACAGCCACGCCCCGCCTATCGCCAATAGCATAATCGCCAGCGTATAGCGGAACAGc < 2:604849/99‑1 (MQ=255) gCGTGTTCGAGATCGACCAACGGTCGGTTCTGGATACGAATAAACAGCCACGCCCCGCCTATCGCCAATAGCATAATCGCCAGCGTATAGCGGAACAGc > 2:1835933/1‑99 (MQ=255) cGTGTTCGAGATCGACCAACGGTCGGTTCTGGATACGAATAAACAGCCACGCCCCGCCTATCGCCAATAGCATAATCGCCAGCGTATAGCGGAACAGCg < 2:1236648/99‑1 (MQ=255) cGTGTTCGAGATCGACCAACGGTCGGTTCTGGATACGAATAAACAGCCACGCCCCGCCTATCGCCAATAGCATAATCGCCAGCGTATAGCGGAACAGCg < 2:1023901/99‑1 (MQ=255) tgtTCGAGATCGACCAACGGTCGGTTCTGGATACGAATAAACAGCCACGCCCCGCCTATCGCCAATAGCATAATCGCCAGCGTATAGCGGAACAGCGga > 1:270162/1‑99 (MQ=255) ttCGAGATCGACCAACAGTCGGTTCTGGATACGAATAAACAGCCACGCCCCGCCTATCGCCAATAGCATAATCGCCAGCGTATAGCGGAACAGCGgaga > 1:476861/1‑99 (MQ=255) agaTCGACCAACGGTCGGTTCTGGATACGAATAAACAGCCACGCCCCGCCTATCGCCAATAGCATAATCGCCAGCGTATAGCGGAACAGCGGAGAGaaa > 2:866869/1‑99 (MQ=255) agaTCGACCAACGGTCGGTTCTGGATACGAATAAACAGCCACGCCCCGCCTATCGCCAATAGCATAATCGCCAGCGTATAGCGGAACAGCGGAGAGaaa > 2:1448153/1‑99 (MQ=255) tCGACCAACGGTCGGTTCTGGATACGAATAAACAGCCACGCCCCGCCTATCGCCAATAGCATAATCGCCAGCGTATAGCGGAACAGCGGAGAGAAATCg < 2:1961325/99‑1 (MQ=255) aCCAACGGTCGGTTCTGGATACGAATAAACAGCCACGCCCCGCCTATCGCCAATAGCATAATCGCCAGCGTATAGCGGAACAGCGGAGAGAAATAGccc > 1:905822/1‑99 (MQ=255) ccAACAGTCGGTTCTGGATACGAATAAACAGCCACGCCCCGCCTATCGCCAATAGCATAATCGCCAGCGTATAGCGGAACAGCGGAGAGAAATCGCCCt < 1:2083796/99‑1 (MQ=255) aaCGGTCGGTTCTGGATACGAATAAACAGCCACGCCCCGCCTATCGCCAATAGCATAATCGCCAGCGTATAGCGGAACAGCGGAGAGAAATAGCCCtga < 1:947369/99‑1 (MQ=255) aaCGGTCGGTTCTGGATACGAATAAACAGCCACGCCCCGCCTATCGCCAATAGCATAATCGCCAGCGTATAGCGGAACAGCGGAGAGAAATAGCCCtga > 1:187639/1‑99 (MQ=255) | GGTAACGGAACGCACCTCCGAAGCGCCATACTCACGCAGCGGCGGCGGAATAATCCCTTTACCAACCTGCAAGGCTGCGTGTTCGAGATCGACCAACGGTCGGTTCTGGATACGAATAAACAGCCACGCCCCGCCTATCGCCAATAGCATAATCGCCAGCGTATAGCGGAACAGCGGAGAGAAATCGCCCTGA > NC_000913/3535218‑3535410 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |