| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 2,163,369 | A→C | 100% | Q165P (CAA→CCA) | baeS → | sensory histidine kinase BaeS |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,163,369 | 0 | A | C | 97.2% | 285.3 / ‑0.0 | 72 | Q165P (CAA→CCA) | baeS | sensory histidine kinase BaeS |
| Reads supporting (aligned to +/- strand): ref base A (2/0); new base C (33/37); total (35/37) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.33e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.16e-01 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
TCTGGTCAACGGTGCGGAAGTTGGCGCGGTGATCGCCTCCCCCGTTGAGCGGTTAACGCGCAATACTGATATCAATTTCGATAAACAACAGCGGCAAACCAGCTGGTTGATTGTCGCCCTGGCAACGTTACTCGCGGCACTTGCCACTTTTCTGCTGGCGCGCGGTTTACTGGCACCGGTAAAACGAC > NC_000913/2163274‑2163461 | tCTGGTCAACGGTGCGGAAGTTGGCGCGGTGATCGCCTCCCCCGTTGAGCGGTTAACGCGCAATACTGATATCAATTTCGATAAACAACAGCGGCCAAc < 1:539737/99‑1 (MQ=255) tGGTCAACGGTGCGGAAGTTGGCGCGGTGATCGCCTCCCCCGTTGAGCGGTTAACGCGCAATACTGATATCAATTTCGATAAACAACAGCGGCCAACCa > 2:899165/1‑99 (MQ=255) ggTCAACGGTGCGGAAGTTGGCGCGGTGATCGCCTCCCCCGTTGAGCGGTTAACGCGCAATACTGATATCAATTTCGATAAACAACAGCGGCCAACCAg > 2:245335/1‑99 (MQ=255) aCGGTGCGGAAGTTGGCGCGGTGATCGCCTCCCCCGTTGAGCGGTTAACGCGCAATACTGATATCAATTTCGATAAACAACAGCGGCCAACCAGCTGGt < 2:1639407/99‑1 (MQ=255) gTGCGGAAGTTGGCGCGGTGATCGCCTCCCCCGTTGAGCGGTTAACGCGCAATACTGATATCAATTTCGATAAACAACAGCGGCCAACCAGCTGGTTGa > 2:1980154/1‑99 (MQ=255) gTGCGGAAGTTGGCGCGGTGATCGCCTCCCCCGTTGAGCGGTTAACGCGCAATACTGATATCAATTTCGATAAACAACAGCGGCCAACCAGCTGGTTGa > 1:1968519/1‑99 (MQ=255) gTGCGGAAGTTGGCGCGGTGATCGCCTCCCCCGTTGAGCGGTTAACGCGCAATACTGATATCAATTTCGATAAACAACAGCGGCCAACCAGCTGGTTGa < 2:455437/99‑1 (MQ=255) tGCGGAAGTTGGCGCGGTGATCGCCTCCCCCGTTGAGCGGTTAACGCGCAATACTGATATCAATTTCGATAAACAACAGCGGCCAACCAGCTGGTTGAt < 2:2087983/99‑1 (MQ=255) gCGGAAGTTGGCGCGGTGATCGCCTCCCCCGTTGAGCGGTTAACGCGCAATACTGATATCAATTTCGATAAACAACAGCGGCCAACCAGCTGGTTGAtt < 1:1075694/99‑1 (MQ=255) gCGGAAGTTGGCGCGGTGATCGCCTCCCCCGTTGAGCGGTTAACGCGCAATACTGATATCAATTTCGATAAACAACAGCGGCCAACCAGCTGGTTGAtt < 1:1032526/99‑1 (MQ=255) cGGAAGTTGGCGCGGTGATCGCCTCCCCCGTTGAGCGGTTAACGCGCAATACTGATATCAATTTCGATAAACAACAGCGGCCAACCAGCTGGTTGATTg < 1:1202997/99‑1 (MQ=255) ggAAGTTGGCGCGGTGATCGCCTCCCCCGTTGAGCGGTTAACGCGCAATACTGATATCAATTTCGATAAACAACAGCGGCCAACCAGCTGGTTGATTGt > 2:333411/1‑99 (MQ=255) gAAGTTGGCGCGGTGATCGCCTCCCCCGTTGAGCGGTTAACGCGCAATACTGATATCAATTTCGATAAACAACAGCGGCCAACCAGCTGGTTGATTGTc > 2:626612/1‑99 (MQ=255) aGTTGGCGCGGTGATCGCCTCCCCCGTTGAGCGGTTAACGCGCAATACTGATATCAATTTCGATAAACAACAGCGGCCAACCAGCTGGTTGATTGTCGc > 1:79838/1‑99 (MQ=255) gcgcGGTGATCGCCTCCCCCGTTGAGCGGTTAACGCGCAATACTGATATCAATTTCGATAAACAACAGCGGCCAACCAGCTGGTTGATTGTCGCCCTgg < 1:1443607/99‑1 (MQ=255) gcgGTGATCGCCTCCCCCGTTGAGCGGTTAACGCGCAATACTGATATCAATTTCGATAAACAACAGCGGCCAACCAGCTGGTTGATTGTCGCCCTGGCa > 2:287559/1‑99 (MQ=255) ggTGATCGCCTCCCCCGTTGAGCGGTTAACGCGCAATACTGATATCAATTTCGATAAACAACAGCGGCCAACCAGCTGGTTGATTGTCGCCCTGGCAAc < 2:697300/99‑1 (MQ=255) ggTGATCGCCTCCCCCGTTGAGCGGTTAACGCGCAATACTGATATCAATTTCGATAAACAACAGCGGCCAACCAGCTGGTTGATTGTCGCCCTGGCAAc < 2:1196197/99‑1 (MQ=255) ggTGATCGCCTCCCCCGTTGAGCGGTTAACGCGCAATACTGATATCAATTTCGATAAACAACAGCGGCCAACCAGCTGGTTGATTGTCGCCCTGGCAAc < 1:818381/99‑1 (MQ=255) gTGATCGCCTCCCCCGTTGAGCGGTTAACGCGCAATACTGATATCAATTTCGATAAACAACAGCGGCCAACCAGCTGGTTGATTGTCGCCCTGGCAACg < 1:1134532/99‑1 (MQ=255) tGATCGCCTCCCCCGTTGAGCGGTTAACGCGCAATACTGATATCAATTTCGATAAACAACAGCGGCCAACCAGCTGGTTGATTGTCGCCCTGGCAACGt > 2:2244800/1‑99 (MQ=255) tGATCGCCTCCCCCGTTGAGCGGTTAACGCGCAATACTGATATCAATTTCGATAAACAACAGCGGCCAACCAGCTGGTTGATTGTCGCCCTGGCAACGt < 1:713655/99‑1 (MQ=255) tGATCGCCTCCCCCGTTGAGCGGTTAACGCGCAATACTGATATCAATTTCGATAAACAACAGCGGCCAACCAGCTGGTTGATTGTCGCCCTGGCAACGt > 2:382289/1‑99 (MQ=255) tCGCCTCCCCCGTTGAGCGGTTAACGCGCAATACTGATATCAATTTCGATAAACAACAGCGGCCAACCAGCTGGTTGATTGTCGCCCTGGCAACGTTAc < 2:2146839/99‑1 (MQ=255) cGCCTCCCCCGTTGAGCGGTTAACGCGCAATACTGATATCAATTTCGATAAACAACAGCGGCCAACCAGCTGGTTGATTGTCGCCCTGGCAACGTTACt < 2:1648256/99‑1 (MQ=255) cccccGTTGAGCGGTTAACGCGCAATACTGATATCAATTTCGATAAACAACAGCGGCCAACCAGCTGGTTGATTGTCGCCCTGGCAACGTTACTCGCgg < 2:1606406/99‑1 (MQ=255) ccccGTTGAGCGGTTAACGCGCAATACTGATATCAATTTCGATAAACAACAGCGGCCAACCAGCTGGTTGATTGTCGCCCTGGCAACGTTACTCGCGGc > 2:261651/1‑99 (MQ=255) ccccGTTGAGCGGTTAACGCGCAATACTGATATCAATTTCGATAAACAACAGCGGCCAACCAGCTGGTTGATTGTCGCCCTGGCAACGTTACTCGCGGc > 2:963768/1‑99 (MQ=255) cccGTTGAGCGGTTAACGCGCAATACTGATATCAATTTCGATAAACAACAGCGGCCAACCAGCTGGTTGATTGTCGCCCTGGCAACGTTACTCGCGGCa > 1:1654992/1‑99 (MQ=255) ccGTTGAGCGGTTAACGCGCAATACTGATATCAATTTCGATAAACAACAGCGGCCAACCAGCTGGTTGATTGTCGCCCTGGCAACGTTACTCGCGGCAc < 1:756441/99‑1 (MQ=255) cGTTGAGCGGTTAACGCGCAATACTGATATCAATTTCGATAAACAACAGCGGCCAACCAGCTGGTTGATTGTCGCCCTGGCAACGTTACTCGCGGCACt > 1:1473083/1‑99 (MQ=255) cGTTGAGCGGTTAACGCGCAATACTGATATCAATTTCGATAAACAACAGCGGCCAACCAGCTGGTTGATTGTCGCCCTGGCAACGTTACTCGCGGCACt < 1:2114468/99‑1 (MQ=255) cGTTGAGCGGTTAACGCGCAATACTGATATCAATTTCGATAAACAACAGCGGCCAACCAGCTGGTTGATTGTCGCCCTGGCAACGTTACTCGCGGCACt > 2:2052624/1‑99 (MQ=255) gTTGAGCGGTTAACGCGCAATACTGATATCAATTTCGATAAACAACAGCGGCCAACCAGCTGGTTGATTGTCGCCCTGGCAACGTTACTCGCGGCACtt < 2:205711/99‑1 (MQ=255) gTTGAGCGGTTAACGCGCAATACTGATATCAATTTCGATAAACAACAGCGGCCAACCAGCTGGTTGATTGTCGCCCTGGCAACGTTACTCGCGGCACtt < 2:1889116/99‑1 (MQ=255) gCGGTTAACGCGCAATACTGATATCAATTTCGATAAACAACAGCGGCCAACCAGCTGGTTGATTGTCGCCCTGGCAACGTTACTCGCGGCACTTGCCAc < 1:256484/99‑1 (MQ=255) gTTAACGCGCAATACTGATATCAATTTCGATAAACAACAGCGGCCAACCAGCTGGTTGATTGTCGCCCTGGCAACGTTACTCGCGGCACTTGCCACttt > 1:827193/1‑99 (MQ=255) gTTAACGCGCAATACTGATATCAATTTCGATAAACAACAGCGGCCAACCAGCTGGTTGATTGTCGCCCTGGCAACGTTACTCGCGGCACTTGCCACttt < 1:1407315/99‑1 (MQ=255) gTTAACGCGCAATACTGATATCAATTTCGATAAACAACAGCGGCCAACCAGCTGGTTGATTGTCGCCCTGGCAACGTTACTCGCGGCACTTGCCACttt > 1:2167161/1‑99 (MQ=255) tAACGCGCAATACTGATATCAATTTCGATAAACAACAGCGGCCAACCAGCTGGTTGATTGTCGCCCTGGCAACGTTACTCGCGGCACTTGCCACTTTTc < 2:855930/99‑1 (MQ=255) tAACGCGCAATACTGATATCAATTTCGATAAACAACAGCGGCCAACCAGCTGGTTGATTGTCGCCCTGGCAACGTTACTCGCGGCACTTGCCACTTTTc < 2:2128768/99‑1 (MQ=255) tAACGCGCAATACTGATATCAATTTCGATAAACAACAGCGGCCAACCAGCTGGTTGATTGTCGCCCTGGCAACGTTACTCGCGGCACTTGCCACTTTTc < 2:2116937/99‑1 (MQ=255) aCGCGCAATACTGATATCAATTTCGATAAACAACAGCGGCCAACCAGCTGGTTGATTGTCGCCCTGGCAACGTTACTCGCGGCACTTGCCACTTTTctg > 1:1177091/1‑99 (MQ=255) gcAATACTGATATCAATTTCGATAAACAACAGCGGCCAACCAGCTGGTTGATTGTCGCCCTGGCAACGTTACTCGCGGCACTTGCCACTTTTCTGCTgg < 2:855570/99‑1 (MQ=255) cAATACTGATATCAATTTCGATAAACAACAGCGGCCAACCAGCTGGTTGATTGTCGCCCTGGCAACGTTACTCGCGGCACTTGCCACTTTTCTGCTGgc > 1:1897532/1‑99 (MQ=255) aTACTGATATCAATTTCGATAAACAACAGCGGCAAACCAGCTGGTTGATTGTCGCCCTGGCAACGTTACTCGCGGCACTTGCCACTTTTCTGCTGgcgc > 2:1523339/1‑99 (MQ=255) tACTGATATCAATTTCGATAAACAACAGCGGCCAACCAGCTGGTTGATTGTCGCCCTGGCAACGTTACTCGCGGCACTTGCCACTTTTCTGCTGgcgcg > 2:2203955/1‑99 (MQ=255) aCTGATATCAATTTCGATAAACAACAGCGGCCAACCAGCTGGTTGATTGTCGCCCTGGCAACGTTACTCGCGGCACTTGCCACTTTTCTGCTGgcgcgc > 2:335192/1‑99 (MQ=255) cTGATATCAATTTCGATAAACAACAGCGGCCAACCAGCTGGTTGATTGTCGCCCTGGCAACGTTACTCGCGGCACTTGCCACTTTTCTGCTGGcgcgcg > 2:1859955/1‑99 (MQ=255) cTGATATCAATTTCGATAAACAACAGCGGCCAACCAGCTGGTTGATTGTCGCCCTGGCAACGTTACTCGCGGCACTTGCCACTTTTCTGCTGGcgcgcg > 2:1228239/1‑99 (MQ=255) cTGATATCAATTTCGATAAACAACAGCGGCCAACCAGCTGGTTGATTGACGCCCTGGCAACGTTACTCGCGGCACTTGCCACTTTTCTGCTGGcgcgcg > 2:373291/1‑99 (MQ=255) tGATATCAATTTCGATAAACAACAGCGGCCAACCAGCTGGTTGATTGTCGCCCTGGCAACGTTACTCGCGGCACTTGCCACTTTTCTGCTGGCGCGCgg < 2:1789941/99‑1 (MQ=255) tGATAGCAATTTCGATAAACAACAGCGGCCATCCAGCTGGTTGATTGTCGCCCTGGCAACGTTACTCGCGGCACTTGCCACTTTTCTGCTGGCGCGCgg < 2:2132631/99‑1 (MQ=255) gATATCAATTTCGATAAACAACAGCGGCCAACCAGCTGGTTGATTGTCGCCCTGGCAACGTTACTCGCGGCACTTGCCACTTTTCTGCTGGCGCGCGGt > 2:1980169/1‑99 (MQ=255) atCAATTTCGATAAACAACAGCGGCCAACCAGCTGGTTGATTGTCGCCCTGGCAACGTTACTCGCGGCACTTGCCACTTTTCTGCTGGCGCGCGGTTTa > 1:1716993/1‑99 (MQ=255) tCAATTTCGTTTGACAACAGCGGACAACCCGCTGGTTGATTGTCGCCCTGGAAACGTTACCCGCGGCACTTGCGACTTTTCTGCTGGCGCGCAGTTTAc < 2:1260670/99‑1 (MQ=255) tCAATTTCGATAAACAACAGCGGCCAACCAGCTGGTTGATTGTCGCCCTGGCAACGTTACTCGCGGCACTTGCCACTTTTCTGCTGGCGCGCGGTTTAc < 1:860207/99‑1 (MQ=255) tCAATTTCGATAAACAACAGCGGCCAACCAGCTGGTTGATTGTCGCCCTGGCAACGTTACTCGCGGCACTTGCCACTTTTCTGCTGGCGCGCGGTTTAc < 2:2221682/99‑1 (MQ=255) tCAATTTCGATAAACAACAGCGGCCAACCAGCTGGTTGATTGTCGCCCTGGCAACGTTACTCGCGGCACTTGCCACTTTTCTGCTGGCGCGCGGTTTAc > 1:1303838/1‑99 (MQ=255) cAATTTCGATAAACAACAGCGGCCAACCAGCTGGTTGATTGTCGCCCTGGCAACGTTACTCGCGGCACTTGCCACTTTTCTGCTGGCGCGCGGTTTACt < 1:1711324/99‑1 (MQ=255) aaTTTCGATAAACAACAGCGGCCAACCAGCTGGTTGATTGTCGCCCTGGCAACGTTACTCGCGGCACTTGCCACTTTTCTGCTGGCGCGCGGTTTACTg < 1:1778850/99‑1 (MQ=255) tCGATAAACAACAGCGGCCAACCAGCTGGTTGATTGTCGCCCTGGCAACGTTACTCGCGGCACTTGCCACTTTTCTGCTGGCGCGCGGTTTACTGGCAc > 1:1707134/1‑99 (MQ=255) gATAAACAACAGCGGCCAACCAGCTGGTTGATTGTCGCCCTGGCAACGTTACTCGCGGCACTTGCCACTTTTCTGCTGGCGCGCGGTTTACTGGCACCg > 1:1505952/1‑99 (MQ=255) aTAAACAACAGCGGCCAACCAGCTGGTTGATTGTCGCCCTGGCAACGTTACTCGCGGCACTTGCCACTTTTCTGCTGGCGCGCGGTTTACTGGCACCgg < 2:444256/99‑1 (MQ=255) tAAACAACAGCGGCCAACCAGCTGGTTGATTGTCGCCCTGGCAACGTTACTCGCGGCACTTGCCACTTTTCTGCTGGCGCGCGGTTTACTGGCACCGGt > 2:1702363/1‑99 (MQ=255) aaaCAACAGCGGCAAACCAGCTGGTTGATTGTCGCCCTGGCAACGTTACTCGCGGCACTTGCCACTTTTCTGCTGGCGCGCGGTTTACTGGCACCGGTa > 1:367576/1‑99 (MQ=255) acaacaGCGGCCAACCAGCTGGTTGATTGTCGCCCTGGCAACGTTACTCGCGGCACTTGCCACTTTTCTGCTGGCGCGCGGTTTACTGGCACCGGTaaa > 2:2000537/1‑99 (MQ=255) aacaGCGGCCAACCAGCTGGTTGATTGTCGCCCTGGCAACGTTACTCGCGGCACTTGCCACTTTTCTGCTGGCGCGCGGTTTACTGGCACCGGTAAAAc < 2:1465729/99‑1 (MQ=255) caGCGGCCAACCAGCTGGTTGATTGTCGCCCTGGCAACGTTACTCGCGGCACTTGCCACTTTTCTGCTGGCGCGCGGTTTACTGGCACCGGTAAAACGa < 1:1965811/99‑1 (MQ=255) caGCGGCCAACCAGCTGGTTGATTGTCGCCCTGGCAACGTTACTCGCGGCACTTGCCACTTTTCTGCTGGCGCGCGGTTTACTGGCACCGGTAAAACGa > 1:474108/1‑99 (MQ=255) caGCGGCCAACCAGCTGGTTGATTGTCGCCCTGGCAACGTTACTCGCGGCACTTGCCACTTTTCTGCTGGCGCGCGGTTTACTGGCACCGGTAAAACGa > 1:483484/1‑99 (MQ=255) aGCGGCCAACCAGCTGGTTGATTGTCGCCCTGGCAACGTTACTCGCGGCACTTGCCACTTTTCTGCTGGCGCGCGGTTTACTGGCACCGGTAAAACGAc < 1:447220/99‑1 (MQ=255) | TCTGGTCAACGGTGCGGAAGTTGGCGCGGTGATCGCCTCCCCCGTTGAGCGGTTAACGCGCAATACTGATATCAATTTCGATAAACAACAGCGGCAAACCAGCTGGTTGATTGTCGCCCTGGCAACGTTACTCGCGGCACTTGCCACTTTTCTGCTGGCGCGCGGTTTACTGGCACCGGTAAAACGAC > NC_000913/2163274‑2163461 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |