Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 486,374 G→T 12.2% C205F (TGC→TTC)  acrR → DNA‑binding transcriptional repressor AcrR

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913486,3740GT12.2% 570.2 / 53.8 155C205F (TGC→TTC) acrRDNA‑binding transcriptional repressor AcrR
Reads supporting (aligned to +/- strand):  ref base G (63/73);  new base T (9/10);  total (72/83)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 162 total aligned reads displayed.

CGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCTTTTGATCTTAAAAAAGAAGCCCGCGATTACGTTGCCATCTTACTGGAGATGTATCTCCTGTGCCCCACGCTTCGTAATCCTGCCACTAACGAATAACCCTGAATCTGACTCCAGGATTTTTCCTGGACATTTTCGTCGTTGCTATTCTGGTTCACTGCGT  >  NC_000913/486276‑486472
                                                                                                  |                                                                                                  
  gCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCTTTTGATCTTAAAAAAGAAGCCCGCGATTACGTTGCCATCTTACTGGAGATGTATCTCCTGTGcc                                                                                                  >  2:3046735/1‑99 (MQ=255)
  gCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCTTTTGATCTTAAAAAAGAAGCCCGCGATTACGTTGCCATCTTACTGGAGATGTATCTCCTGTGcc                                                                                                  <  2:115482/99‑1 (MQ=255)
   ccTGATGGAAAACTGGCTCTTTGCCCCGCAATCTTTTGATCTTAAAAAAGAAGCCCGCGATTACGTTGCCATCTTACTGGAGATGTATCTCCTGTTccc                                                                                                 <  1:3069459/99‑1 (MQ=255)
     tGATGGAAAACTGGCTCTTTGCCCCGCAATCTTTTGATCTTAAAAAAGAAGCCCGCGATTACGTTGCCATCTTACTGGAGATGTATCTCCTGTTCCCCa                                                                                               <  2:277169/99‑1 (MQ=255)
     tGATGGAAAACTGGCTCTTTGCCCCGCAATCTTTTGATCTTAAAAAAGAAGCCCGCGATTACGTTGCCATCTTACTGGAGATGTATCTCCTGTGCCCCa                                                                                               <  2:2918997/99‑1 (MQ=255)
        tGGAAAACTGGCTCTTTGCCCCGCAATCTTTTGATCTTAAAAAAGAAGCCCGCGATTACGTTGCCATCTTACTGGAGATGTATCTCCTGTGCCCCACGc                                                                                            >  1:2771950/1‑99 (MQ=255)
          gAAAACTGGCTCTTTGCCCCGCAATCTTTTGATCTTAAAAAAGAAGCCCGCGATTACGTTGCCATCTTACTGGAGATGTATCTCCTGTGCCCCACGCtt                                                                                          >  2:769905/1‑99 (MQ=255)
          gAAAACTGGCTCTTTGCCCCGCAATCTTTTGATCTTAAAAAAGAAGCCCGCGATTACGTTGCCATCTTACTGGAGATGTATCTCCTGTGCCCCACGCtt                                                                                          <  2:273722/99‑1 (MQ=255)
           aaaaCTGGCTCTTTGCCCCGCAATCTTTTGATCTTAAAAAAGAAGCCCGCGATTACGTTGCCATCTTACTGGAGATGTATCTCCTGTGCCCCACGCTTc                                                                                         >  1:873829/1‑99 (MQ=255)
           aaaaCTGGCTCTTTGCCCCGCAATCTTTTGATCTTAAAAAAGAAGCCCGCGATTACGTTGCCATCTTACTGGAGATGTATCTCCTGTGCCCCACGCTTc                                                                                         >  1:786849/1‑99 (MQ=255)
            aaaCTGGCTCTTTGCCCCGCAATCTTTTGATCTTAAAAAAGAAGCCCGCGATTACGTTGCCATCTTACTGGAGATGTATCTCCTGTGCCCCACGCTTCg                                                                                        <  2:2485511/99‑1 (MQ=255)
             aaCTGGCTCTTTGCCCCGCAATCTTTTGATCTTAAAAAAGAAGCCCGCGATTACGTTGCCATCTTACTGGAGATGTATCTCCTGTGCCCCACGCTTCGt                                                                                       >  2:1255721/1‑99 (MQ=255)
             aaCTGGCTCTTTGCCCCGCAATCTTTTGATCTTAAAAAAGAAGCCCGCGATTACGTTGCCATCTTACTGGAGATGTATCTCCTGTGCCCCACGCTTCGt                                                                                       >  1:1412132/1‑99 (MQ=255)
              aCTGGCTCTTTGCCCCGCAATCTTTTGATCTTAAAAAAGAAGCCCGCGATTACGTTGCCATCTTACTGGAGATGTATCTCCTGTGCCCCACGCTTCGTa                                                                                      <  1:727905/99‑1 (MQ=255)
                tGGCTCTTTGCCCCGCAATCTTTTGATCTTAAAAAAGAAGCCCGCGATTACGTTGCCATCTTACTGGAGATGTATCTCCTGTGCCCCACGCTTCGTAAt                                                                                    <  2:2809561/99‑1 (MQ=255)
                  gCTCTTTGCCCCGCAATCTTTTGATCTTAAAAAAGAAGCCCGCGATTACGTTGCCATCTTACTGGAGATGTATCTCCTGTGCCCCACGCTTCGTAATcc                                                                                  >  2:1743668/1‑99 (MQ=255)
                  gCTCTTTGCCCCGCAATCTTTTGATCTTAAAAAAGAAGCCCGCGATTACGTTGCCATCTTACTGGAGATGTATCTCCTGTGCCCCACGCTTCGTAATcc                                                                                  >  1:1113916/1‑99 (MQ=255)
                   ctctTTGCCCCGCAATCTTTTGATCTTAAAAAAGAAGCCCGCGATTACGTTGCCATCTTACTGGAGATGTATCTCCTGTGCCCCACGCTTCGTAATCCt                                                                                 >  2:2747739/1‑99 (MQ=255)
                     ctTTGCCCCGCAATCTTTTGATCTTAAAAAAGAAGCCCGCGATTACGTTGCCATCTTACTGGAGATGTATCTCCTGTGCCCCACGCTTCGTAATCCTGc                                                                               >  1:625332/1‑99 (MQ=255)
                     ctTTGCCCCGCAATCTTTTGATCTTAAAAAAGAAGCCCGCGATTACGTTGCCATCTTACTGGAGATGTATCTCCTGTGCCCCACGCTTCGTAATCCTGc                                                                               <  1:2032126/99‑1 (MQ=255)
                     ctTTGCCCCGCAATCTTTTGATCTTAAAAAAGAAGCCCGCGATTACGTTGCCATCTTACTGGAGATGTATCTCCTGTGCCCCACGCTTCGTAATCCTGc                                                                               <  1:2404142/99‑1 (MQ=255)
                      tttGCCCCGCAATCTTTTGATCTTAAAAAAGAAGCCCGCGATTACGTTGCCATCTTACTGGAGATGTATCTCCTGTGCCCCACGCTTCGTAATCCTGcc                                                                              <  1:2948081/99‑1 (MQ=255)
                      tttGCCCCGCAATCTTTTGATCTTAAAAAAGAAGCCCGCGATTACGTTGCCATCTTACTGGAGATGTATCTCCTGTGCCCCACGCTTCGTAATCCTGcc                                                                              >  1:324882/1‑99 (MQ=255)
                          ccccGCAATCTTTTGATCTTAAAAAAGAAGCCCGCGATTACGTTGCCATCTTACTGGAGATGTATCTCCTGTGCCCCACGCTTCGTAATCCTGCCACTa                                                                          >  2:685076/1‑99 (MQ=255)
                           cccGCAATCTTTTGATCTTAAAAAAGAAGCCCGCGATTACGTTGCCATCTTACTGGAGATGTATCTCCTGTTCCCCACGCTTCGTAATCCTGCCACTaa                                                                         >  1:1893931/1‑99 (MQ=255)
                               cAATCTTTTGATCTTAAAAAAGAAGCCCGCGATTACGTTGCCATCTTACTGGAGATGTATCTCCTGTGCCCCACGCTTCGTAATCCTGCCACTAACGaa                                                                     <  1:3372612/99‑1 (MQ=255)
                               cAATCTTTTGATCTTAAAAAAGAAGCCCGCGATTACGTTGCCATCTTACTGGAGATGTATCTCCTGTGCCCCACGCTTCGTAATCCTGCCACTAACGaa                                                                     <  1:1688831/99‑1 (MQ=255)
                               cAATCTTTTGATCTTAAAAAAGAAGCCCGCGATTACGTTGCCATCTTACTGGAGATGTATCTCCTGTGCCCCACGCTTCGTAATCCTGCCACTAACGaa                                                                     >  1:663019/1‑99 (MQ=255)
                               cAATCTTTTGATCTTAAAAAAGAAGCCCGCGATTACGTTGCCATCTTACTGGAGATGTATCTCCTGTGCCCCACGCTTCGTAATCCTGCCACTAACGaa                                                                     >  2:1177021/1‑99 (MQ=255)
                                aaTCTTTTGATCTTAAAAAAGAAGCCCGCGATTACGTTGCCATCTTACTGGAGATGTATCTCCTGTGCCCCACGCTTCGTAATCCTGCCACTAACGAAt                                                                    <  2:1334956/99‑1 (MQ=255)
                                 aTCTTTTGATCTTAAAAAAGAAGCCCGCGATTACGTTGCCATCTTACTGGAGATGTATCTCCTGTGCCCCACGCTTCGTAATCCTGCCACTAACGAATa                                                                   >  1:761155/1‑99 (MQ=255)
                                 aTCTTTTGATCTTAAAAAAGAAGCCCGCGATTACGTTGCCATCTTACTGGAGATGTATCTCCTGTGCCCCACGCTTCGTAATCCTGCCACTAACGAATa                                                                   >  2:2383081/1‑99 (MQ=255)
                                  tCTTTTGATCTTAAAAAAGAAGCCCGCGATTACGTTGCCATCTTACTGGAGATGTATCTCCTGTGCCCCACGCTTCGTAATCCTGCCACTAACGAATaa                                                                  >  1:1321592/1‑99 (MQ=255)
                                    ttttGATCTTAAAAAAGAAGCCCGCGATTACGTTGCCATCTTACTGGAGATGTATCTCCTGTGCCCCACGCTTCGTAATCCTGCCACTAACGAATAAcc                                                                >  1:3362637/1‑99 (MQ=255)
                                     tttGATCTTAAAAAAGAAGCCCGCGATTACGTTGCCATCTTACTGGAGATGTATCTCCTGTGCCCCACGCTTCGTAATCCTGCCACTAACGAATAAccc                                                               <  2:763232/99‑1 (MQ=255)
                                      ttGATCTTAAAAAAGAAGCCCGCGATTACGTTGCCATCTTACTGGAGATGTATCTCCTGTGCCCCACGCTTCGTAATCCTGCCACTAACGAATAACCCt                                                              >  2:1409962/1‑99 (MQ=255)
                                      ttGATCTTAAAAAAGAAGCCCGCGATTACGTTGCCATCTTACTGGAGATGTATCTCCTGTGCCCCACGCTTCGTAATCCTGCCACTAACGAATAACCCt                                                              <  2:1925971/99‑1 (MQ=255)
                                         aTCTTAAAAAAGAAGCCCGCGATTACGTTGCCATCTTACTGGAGATGTATCTCCTGTGCCCCACGCTTCGTAATCCTGCCACTAACGAATAACCCTGaa                                                           >  2:1928904/1‑99 (MQ=255)
                                          tCTTAAAAAAGAAGCCCGCGATTACGTTGCCATCTTACTGGAGATGTATCTCCTGTGCCCCACGCTTCGTAATCCTGCCACTAACGAATAACCCTGAAt                                                          <  1:1934038/99‑1 (MQ=255)
                                           cTTAAAAAAGAAGCCCGCGATTACGTTGCCATCTTACTGGAGATGTATCTCCTGTTCCCCACGCTTCGTAATCCTGCCACTAACGAATAACCCTGAATc                                                         >  1:226881/1‑99 (MQ=255)
                                           cTTAAAAAAGAAGCCCGCGATTACGTTGCCATCTTACTGGAGATGTATCTCCTGTGCCCCACGCTTCGTAATCCTGCCACTAACGAATAACCCTGAATc                                                         <  2:1386821/99‑1 (MQ=255)
                                           cTTAAAAAAGAAGCCCGCGATTACGTTGCCATCTTACTGGAGATGTATCTCCTGTGCCCCACGCTTCGTAATCCTGCCACTAACGAATAACCCTGAATc                                                         <  1:1642663/99‑1 (MQ=255)
                                           cTTAAAAAAGAAGCCCGCGATTACGTTGCCATCTTACTGGAGATGTATCTCCTGTGCCCCACGCTTCGTAATCCTGCCACTAACGAATAACCCTGAATc                                                         >  1:1582606/1‑99 (MQ=255)
                                            ttAAAAAAGAAGCCCGCGATTACGTTGCCATCTTACTGGAGATGTATCTCCTGTGCCCCACGCTTCGTAATCCTGCCACTAACGAATAACCCTGAATCt                                                        <  2:1631539/99‑1 (MQ=255)
                                              aaaaaaGAAGCCCGCGATTACGTTGCCATCTTACTGGAGATGTATCTCCTGTTCCCCACGCTTCGTAATCCTGCCACTAACGAATAACCCTGAATCTGa                                                      <  1:2909385/99‑1 (MQ=255)
                                              aaaaaaGAAGCCCGCGATTACGTTGCCATCTTACTGGAGATGTATCTCCTGTTCCCCACGCTTCGTAATCCTGCCACTAACGAATAACCCTGAATCTGa                                                      <  2:1744948/99‑1 (MQ=255)
                                               aaaaaGAAGCCCGCGATTACGTTGCCATCTTACTGGAGATGTATCTCCTGTGCCCCACGCTTCGTAATCCTGCCACTAACGAATAACCCTGAATCTGAc                                                     >  1:2783606/1‑99 (MQ=255)
                                                aaaaGAAGCCCGCGATTACGTTGCCATCTTACTGGAGATGTATCTCCTGTGCCCCACGCTTCGTAATCCTGCCACTAACGAATAACCCTGAATCTGACt                                                    >  2:259209/1‑99 (MQ=255)
                                                 aaaGAAGCCCGCGATTACGTTGCCATCTTACTGGAGATGTATCTCCTGTGCCCCACGCTTCGTAATCCTGCCACTAACGAATAACCCTGAATCTGACTc                                                   <  2:2340479/99‑1 (MQ=255)
                                                 aaaGAAGCCCGCGATTACGTTGCCATCTTACTGGAGATGTATCTCCTGTGCCCCACGCTTCGTAATCCTGCCACTAACGAATAACCCTGAATCTGACTc                                                   >  1:2865141/1‑99 (MQ=255)
                                                  aagaagCCCGCGATTACGTTGCCATCTTACTGGAGATGTATCTCCTGTGCCCCACGCTTCGTAATCCTGCCACTAACGAATAACCCTGAATCTGACTcc                                                  >  1:2183270/1‑99 (MQ=255)
                                                   agaagCCCGCGATTACGTTGCCATCTTACTGGAGATGTATCTCCTGTGCCCCACGCTTCGTAATCCTGCCACTAACGAATAACCCTGAATCTGACTCCa                                                 >  2:2906300/1‑99 (MQ=255)
                                                    gaagCCCGCGATTACGTTGCCATCTTACTGGAGATGTATCTCCTGTGCCCCACGCTTCGTAATCCTGCCACTAACGAATAACCCTGAATCTGACTCCAg                                                >  2:130638/1‑99 (MQ=255)
                                                    gaagCCCGCGATTACGTTGCCATCTTACTGGAGATGTATCTCCTGTGCCCCACGCTTCGTAATCCTGCCACTAACGAATAACCCTGAATCTGACTCCAg                                                >  2:1788918/1‑99 (MQ=255)
                                                       gCCCGCGATTACGTTGCCATCTTACTGGAGATGTATCTCCTGTGCCCCACGCTTCGTAATCCTGCCACTAACGAATAACCCTGAATCTGACTCCAGGAt                                             <  1:1007987/99‑1 (MQ=255)
                                                       gCCCGCGATTACGTTGCCATCTTACTGGAGATGTATCTCCTGTGCCCCACGCTTCGTAATCCTGCCACTAACGAATAACCCTGAATCTGACTCCAGGAt                                             <  1:2841694/99‑1 (MQ=255)
                                                       gCCCGCGATTACGTTGCCATCTTACTGGAGATGTATCTCCTGTGCCCCACGCTTCGTAATCCTGCCACTAACGAATAACCCTGAATCTGACTCCAGGAt                                             >  1:2678671/1‑99 (MQ=255)
                                                        cccGCGATTACGTTGCCATCTTACTGGAGATGTATCTCCTGTTCCCCACGCTTCGTAATCCTGCCACTAACGAATAACCCTGAATCTGACTCCAGGAtt                                            <  1:915722/99‑1 (MQ=255)
                                                          cgcgATTACGTTGCCATCTTACTGGAGATGTATCTCCTGTGCCCCACGCTTCGTAATCCTGCCACTAACGAATAACCCTGAATCTGACTCCAGGAtttt                                          <  1:1268255/99‑1 (MQ=255)
                                                          cgcgATTACGTTGCCATCTTACTGGAGATGTATCTCCTGTGCCCCACGCTTCGTAATCCTGCCACTAACGAATAACCCTGAATCTGACTCCAGGAtttt                                          >  2:48527/1‑99 (MQ=255)
                                                            cgATTACGTTGCCATCTTACTGGAGATGTATCTCCTGTGCCCCACGCTTCGTAATCCTGCCACTAACGAATAACCCTGAATCTGACTCCAGGATTTTTc                                        <  1:568449/99‑1 (MQ=255)
                                                            cgATTACGTTGCCATCTTACTGGAGATGTATCTCCTGTGCCCCACGCTTCGTAATCCTGCCACTAACGAATAACCCTGAATCTGACTCCAGGATTTTTc                                        <  2:3247029/99‑1 (MQ=255)
                                                             gATTACGTTGCCATCTTACTGGAGATGTATCTCCTGTGCCCCACGCTTCGTAATCCTGCCACTAACGAATAACCCTGAATCTGACTCCAGGATTTTTcc                                       <  1:2392803/99‑1 (MQ=255)
                                                              aTTACGTTGCCATCTTACTGGAGATGTATCTCCTGTGCCCCACGCTTCGTAATCCTGCCACTAACGAATAACCCTGAATCTGACTCCAGGATTTTTCCt                                      >  2:3011264/1‑99 (MQ=255)
                                                               ttACGTTGCCATCTTACTGGAGATGTATCTCCTGTGCCCCACGCTTCGTAATCCTGCCACTAACGAATAACCCTGAATCTGACTCCAGGATTTTTCCTg                                     >  2:54047/1‑99 (MQ=255)
                                                                 aCGTTGCCATCTTACTGGAGATGTATCTCCTGTTCCCCACGCTTCGTAATCCTGCCACTAACGAATAACCCTGAATCTGACTCCAGGATTTTTCCTGGa                                   >  2:3082355/1‑99 (MQ=255)
                                                                 aCGTTGCCATCTTACTGGAGATGTATCTCCTGTGCCCCACGCTTCGTAATCCTGCCACTAACGAATAACCCTGAATCTGACTCCAGGATTTTTCCTGGa                                   <  2:2888473/99‑1 (MQ=255)
                                                                  cGTTGCCATCTTACTGGAGATGTATCTCCTGTGCCCCACGCTTCGTAATCCTGCCACTAACGAATAACCCTGAATCTGACTCCAGGATTTTTCCTGGAc                                  <  1:3071602/99‑1 (MQ=255)
                                                                  cGTTGCCATCTTACTGGAGATGTATCTCCTGTGCCCCACGCTTCGTAATCCTGCCACTAACGAATAACCCTGAATCTGACTCCAGGATTTTTCCTGGAc                                  >  1:520887/1‑99 (MQ=255)
                                                                   gTTGCCATCTTACTGGAGATGTATCTCCTGTGCCCCACGCTTCGTAATCCTGCCACTAACGAATAACCCTGAATCTGACTCCAGGATTTTTCCTGGACa                                 <  1:3213611/99‑1 (MQ=255)
                                                                   gTTGCCATCTTACTGGAGATGTATCTCCTGTGCCCCACGCTTCGTAATCCTGCCACTAACGAATAACCCTGAATCTGACTCCAGGATTTTTCCTGGACa                                 >  1:3350217/1‑99 (MQ=255)
                                                                    ttGCCATCTTACTGGAGATGTATCTCCTGTGCCCCACGCTTCGTAATCCTGCCACTAACGAATAACCCTGAATCTGACTCCAGGATTTTTCCTGGACAt                                >  2:2190854/1‑99 (MQ=255)
                                                                       ccATCTTACTGGAGATGTATCTCCTGTGCCCCACGCTTCGTAATCCTGCCACTAACGAATAACCCTGAATCTGACTCCAGGATTTTTCCTGGACAtttt                             <  2:929081/99‑1 (MQ=255)
                                                                          tCTTACTGGAGATGTATCTCCTGTGCCCCACGCTTCGTAATCCTGCCACTAACGAATAACCCTGAATCTGACTCCAGGATTTTTCCTGGACATTTtcgt                          <  2:1615327/99‑1 (MQ=255)
                                                                          tCTTACTGGAGATGTATCTCCTGTGCCCCACGCTTCGTAATCCTGCCACTAACGAATAACCCTGAATCTGACTCCAGGATTTTTCCTGGACATTTtcgt                          >  1:1821073/1‑99 (MQ=255)
                                                                          tCTTACTGGAGATGTATCTCCTGTGCCCCACGCTTCGTAATCCTGCCACTAACGAATAACCCTGAATCTGACTCCAGGATTTTTCCTGGACATTTtcgt                          <  1:2334382/99‑1 (MQ=255)
                                                                            ttACTGGAGATGTATCTCCTGTTCCCCACGCTTCGTAATCCTGCCACTAACGAATAACCCTGAATCTGACTCCAGGATTTTTCCTGGACATTTtcgtcg                        <  2:2764821/99‑1 (MQ=255)
                                                                            ttACTGGAGATGTATCTCCTGTGCCCCACGCTTCGTAATCCTGCCACTAACGAATAACCCTGAATCTGACTCCAGGATTTTTCCTGGACATTTtcgtcg                        <  1:2490359/99‑1 (MQ=255)
                                                                                tGGAGATGTATCTCCTGTGCCCCACGCTTCGTAATCCTGCCACTAACGAATAACCCTGAATCTGACTCCAGGATTTTTCCTGGACATTTTCGTCGTTGc                    <  2:87330/99‑1 (MQ=255)
                                                                                tGGAGATGTATCTCCTGTGCCCCACGCTTCGTAATCCTGCCACTAACGAATAACCCTGAATCTGACTCCAGGATTTTTCCTGGACATTTTCGTCGTTGc                    <  1:2592607/99‑1 (MQ=255)
                                                                                 ggAGATGTATCTCCTGTTCCCCACGCTTCGTAATCCTGCCACTAACGAATAACCCTGAATCTGACTCCAGGATTTTTCCTGGACATTTTCGTCGTTGCt                   <  2:2728960/99‑1 (MQ=255)
                                                                                   agaTGTATCTCCTGTTCCCCACGCTTCGTAATCCTGCCACTAACGAATAACCCTGAATCTGACTCCAGGATTTTTCCTGGACATTTTCGTCGTTGCTAt                 >  1:2685968/1‑99 (MQ=255)
                                                                                   agaTGTATCTCCTGTGCCCCACGCTTCGTAATCCTGCCACTAACGAATAACCCTGAATCTGACTCCAGGATTTTTCCTGGACATTTTCGTCGTTGCTAt                 >  2:2251255/1‑99 (MQ=255)
                                                                                   agaTGTATCTCCTGTGCCCCACGCTTCGTAATCCTGCCACTAACGAATAACCCTGAATCTGACTCCAGGATTTTTCCTGGACATTTTCGTCGTTGCTAt                 <  2:1490201/99‑1 (MQ=255)
                                                                                   agaTGTATCTCCTGTGCCCCACGCTTCGTAATCCTGCCACTAACGAATAACCCTGAATCTGACTCCAGGATTTTTCCTGGACATTTTCGTCGTTGCTAt                 <  2:2631933/99‑1 (MQ=255)
                                                                                    gaTGTATCTCCTGTGCCCCACGCTTCGTAATCCTGCCACTAACGAATAACCCTGAATCTGACTCCAGGATTTTTCCTGGACATTTTCGTCGTTGCTAtt                <  1:60489/99‑1 (MQ=255)
                                                                                    gaTGTATCTCCTGTGCCCCACGCTTCGTAATCCTGCCACTAACGAATAACCCTGAATCTGACTCCAGGATTTTTCCTGGACATTTTCGTCGTTGCTAtt                >  1:2106119/1‑99 (MQ=255)
                                                                                      tGTATCTCCTGTGCCCCACGCTTCGTAATCCTGCCACTAACGAATAACCCTGAATCTGACTCCAGGATTTTTCCTGGACATTTTCGTCGTTGCTATTCt              >  1:1179432/1‑99 (MQ=255)
                                                                                        tATCTCCTGTGCCCCACGCTTCGTAATCCTGCCACTAACGAATAACCCTGAATCTGACTCCAGGATTTTTCCTGGACATTTTCGTCGTTGCTATTCTgg            >  2:2437572/1‑99 (MQ=255)
                                                                                        tATCTCCTGTGCCCCACGCTTCGTAATCCTGCCACTAACGAATAACCCTGAATCTGACTCCAGGATTTTTCCTGGACATTTTCGTCGTTGCTATTCTgg            <  2:1304239/99‑1 (MQ=255)
                                                                                         aTCTCCTGTGCCCCACGCTTCGTAATCCTGCCACTAACGAATAACCCTGAATCTGACTCCAGGATTTTTCCTGGACATTTTCGTCGTTGCTATTCTGGt           <  1:1786683/99‑1 (MQ=255)
                                                                                         aTCTCCTGTGCCCCACGCTTCGTAATCCTGCCACTAACGAATAACCCTGAATCTGACTCCAGGATTTTTCCTGGACATTTTCGTCGTTGCTATTCTGGt           <  1:1568045/99‑1 (MQ=255)
                                                                                           ctcCTGTGCCCCACGCTTCGTAATCCTGCCACTAACGAATAACCCTGAATCTGACTCCAGGATTTTTCCTGGACATTTTCGTCGTTGCTATTCTGGTTc         >  2:41617/1‑99 (MQ=255)
                                                                                           ctcCTGTGCCCCACGCTTCGTAATCCTGCCACTAACGAATAACCCTGAATCTGACTCCAGGATTTTTCCTGGACATTTTCGTCGTTGCTATTCTGGTTc         <  1:905562/99‑1 (MQ=255)
                                                                                            tcCTGTGCCCCACGCTTCGTAATCCTGCCACTAACGAATAACCCTGAATCTGACTCCAGGATTTTTCCTGGACATTTTCGTCGTTGCTATTCTGGTTCa        <  1:1070133/99‑1 (MQ=255)
                                                                                             ccTGTTCCCCACGCTTCGTAATCCTGCCACTAACGAATAACCCTGAATCTGACTCCAGGATTTTTCCTGGACATTTTCGTCGTTGCTATTCTGGTTCAc       <  1:2031030/99‑1 (MQ=255)
                                                                                             ccTGTGCCCCACGCTTCGTAATCCTGCCACTAACGAATAACCCTGAATCTGACTCCAGGATTTTTCCTGGACATTTTCGTCGTTGCTATTCTGGTTCAc       <  1:599314/99‑1 (MQ=255)
                                                                                              cTGTGCCCCACGCTTCGTAATCCTGCCACTAACGAATAACCCTGAATCTGACTCCAGGATTTTTCCTGGACATTTTCGTCGTTGCTATTCTGGTTCACt      <  1:2216227/99‑1 (MQ=255)
                                                                                                gtgCCCCACGCTTCGTAATCCTGCCACTAACGAATAACCCTGAATCTGACTCCAGGATTTTTCCTGGACATTTTCGTCGTTGCTATTCTGGTTCACTGc    <  2:1251880/99‑1 (MQ=255)
                                                                                                  gCCCCACGCTTCGTAATCCTGCCACTAACGAATAACCCTGAATCTGACTCCAGGATTTTTCCTGGACATTTTCGTCGTTGCTATTCTGGTTCACTGcgt  <  2:2992537/99‑1 (MQ=255)
                                                                                                  |                                                                                                  
CGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCTTTTGATCTTAAAAAAGAAGCCCGCGATTACGTTGCCATCTTACTGGAGATGTATCTCCTGTGCCCCACGCTTCGTAATCCTGCCACTAACGAATAACCCTGAATCTGACTCCAGGATTTTTCCTGGACATTTTCGTCGTTGCTATTCTGGTTCACTGCGT  >  NC_000913/486276‑486472

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑