BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	1,619,375:1	+G	100%	coding (256/435 nt)	marR →	DNA‑binding transcriptional repressor MarR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,619,375	1	.	G	98.0%	400.1 / 1.3	98	coding (256/435 nt)	marR	DNA‑binding transcriptional repressor MarR
Reads supporting (aligned to +/- strand): ref base . (1/1); new base G (44/52); total (45/53)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.58e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 102 total aligned reads displayed.

GTGTATTACTCCGGTTGAACTGAAAAAGGTATTGTCGGTCGACCTGGGAGCACTGACCCGTATGCTGGATCGCCTGGTCTGTAAAGGCTGGGTGGAAA‑GGTT‑GCCGAACCCGAATGACAAGCGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCAATATGTGAACAATGCCATCAATTAGTTGGC  >  NC_000913/1619278‑1619467

gtgtATTACTCCGGTTGAACTGAAAAAGGTATTGTCGGTCGACCTGGGAGCACTGACCCGTATGCTGGATCGCCTGGTCTGTAAAGGCTGGGTGGAAA‑g                                                                                              <  2:2212323/99‑1 (MQ=255)

  gtATTACTCCGGTTGAACTGAAAAAGGTATTGTCGGTCGACCTGGGAGCACTGACCCGTATGCTGGATCGCCTGGTCTGTAAAGGCTGGGTGGAAA‑ggg                                                                                            <  2:78690/99‑2 (MQ=255)

   tATTACTCCGGTTGAACTGAAAAAGGTATTGTCGGTCGACCTGGGAGCACTGACCCGTATGCTGGATCGCCTGGTCTGTAAAGGCTGGGTGGAAA‑gggt                                                                                           <  1:559519/99‑3 (MQ=255)

    aTTACTCCGGTTGAACTGAAAAAGGTATTGTCGGTCGACCTGGGAGCACTGACCCGTATGCTGGATCGCCTGGTCTGTAAAGGCTGGGTGGAAA‑gggtt                                                                                          >  2:1392035/1‑96 (MQ=255)

     ttACTCCGGTTGAACTGAAAAAGGTATTGTCGGTCGACCTGGGAGCACTGACCCGTATGCTGGATCGCCTGGTCTGTAAAGGCTGGGTGGAAA‑GGTT‑Gc                                                                                        <  1:674905/99‑1 (MQ=255)

        cTCCGGTTGAACTGAAAAAGGTATTGTCGGTCGACCTGGGAGCACTGACCCGTATGCTGGATCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGGTT‑GCCg                                                                                      >  2:1832944/1‑99 (MQ=255)

        cTCCGGTTGAACTGAAAAAGGTATTGTCGGTCGACCTGGGAGCACTGACCCGTATGCTGGATCGCCTAGTCTGTAAAGGCTGGGTGGAAAGGGTT‑GCCg                                                                                      <  1:1348899/99‑1 (MQ=255)

          ccGGTTGAACTGAAAAAGGTATTGTCGGTCGACCTGGGAGCACTGACCCGTATGCTGGATCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGGTT‑GCCGaa                                                                                    <  2:2136969/99‑1 (MQ=255)

          ccGGTTGAACTGAAAAAGGTATTGTCGGTCGACCTGGGAGCACTGACCCGTATGCTGGATCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGGTT‑GCCGaa                                                                                    <  2:1540812/99‑1 (MQ=255)

           cGGTTGAACTGAAAAAGGTATTGTCGGTCGACCTGGGAGCACTGACCCGTATGCTGGATCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGGTT‑GCCGAAc                                                                                   >  1:1709381/1‑99 (MQ=255)

            ggTTGAACTGAAAAAGGTATTGTCGGTCGACCTGGGAGCACTGACCCGTATGCTGGATCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGGTT‑GCCGAAcc                                                                                  <  1:1402375/99‑1 (MQ=255)

             gTTGAACTGAAAAAGGTATTGTCGGTCGACCTGGGAGCACTGACCCGTATGCTGGATCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGGTT‑GCCGAAccc                                                                                 >  2:174155/1‑99 (MQ=255)

             gTTGAACTGAAAAAGGTATTGTCGGTCGACCTGGGAGCACTGACCCGTATGCTGGATCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGGTT‑GCCGAAccc                                                                                 >  2:2272227/1‑99 (MQ=255)

             gTTGAACTGAAAAAGGTATTGTCGGTCGACCTGGGAGCACTGACCCGTATGCTGGATCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGGTT‑GCCGAAccc                                                                                 >  1:2453398/1‑99 (MQ=255)

              ttGAACTGAAAAAGGTATTGTCGGTCGACCTGGGAGCACTGACCCGTATGCTGGATCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGGTT‑GCCGAACCCg                                                                                >  1:2567217/1‑99 (MQ=255)

              ttGAACTGAAAAAGGTATTGTCGGTCGACCTGGGAGCACTGACCCGTATGCTGGATCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGGTT‑GCCGAACCCg                                                                                >  1:1887971/1‑99 (MQ=255)

                gAACTGAAAAAGGTATTGTCGGTCGACCTGGGAGCACTGACCCGTATGCTGGATCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGGTT‑GCCGAACCCGaa                                                                              <  1:1959102/99‑1 (MQ=255)

                gAACTGAAAAAGGTATTGTCGGTCGACCTGGGAGCACTGACCCGTATGCTGGATCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGGTT‑GCCGAACCCGaa                                                                              <  1:770697/99‑1 (MQ=255)

                 aaCTGAAAAAGGTATTGTCGGTCGACCTGGGAGCACTGACCCGTATGCTGGATCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGGTT‑GCCGAACCCGAAt                                                                             >  2:210623/1‑99 (MQ=255)

                 aaCTGAAAAAGGTATTGTCGGTCGACCTGGGAGCACTGACCCGTATGCTGGATCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGGTT‑GCCGAACCCGAAt                                                                             >  2:68434/1‑99 (MQ=255)

                  aCTGAAAAAGGTATTGTCGGTCGACCTGGGAGCACTGACCCGTATGCTGGATCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGGTT‑GCCGAACCCGAATg                                                                            >  1:171866/1‑99 (MQ=255)

                  aCTGAAAAAGGTATTGTCGGTCGACCTGGGAGCACTGACCCGTATGCTGGATCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGGTT‑GCCGAACCCGAATg                                                                            <  1:570522/99‑1 (MQ=255)

                  aCTGAAAAAGGTATTGTCGGTCGACCTGGGAGCACTGACCCGTATGCTGGATCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGGTT‑GCCGAACCCGAATg                                                                            >  2:1584903/1‑99 (MQ=255)

                   cTGAAAAAGGTATTGTCGGTCGACCTGGGAGCACTGACCCGTATGCTGGATCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGGTT‑GCCGAACCCGAATGa                                                                           <  1:566710/99‑1 (MQ=255)

                      aaaaaGGTATTGTCGGTCGACCTGGGAGCACTGACCCGTATGCTGGATCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGGTT‑GCCGAACCCGAATGACaa                                                                        <  2:242392/99‑1 (MQ=255)

                       aaaaGGTATTGTCGGTCGACCTGGGAGCACTGACCCGTATGCTGGATCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGGTT‑GCCGAACCCGAATGACAAg                                                                       <  2:1172885/99‑1 (MQ=255)

                       aaaaGGTATTGTCGGTCGACCTGGGAGCACTGACCCGTATGCTGGATCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGGTT‑GCCGAACCCGAATGACAAg                                                                       >  1:2379952/1‑99 (MQ=255)

                       aaaaGGTATTGTCGGTCGACCTGGGAGCACTGACCCGTATGCTGGATCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGGTT‑GCCGAACCCGAATGACAAg                                                                       <  1:23986/99‑1 (MQ=255)

                        aaaGGTATTGTCGGTCGACCTGGGAGCACTGACCCGTATGCTGGATCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGGTT‑GCCGAACCCGAATGACAAgc                                                                      <  1:484655/99‑1 (MQ=255)

                         aaGGTATTGTCGGTCGACCTGGGAGCACTGACCCGTATGCTGGATCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGGTT‑GCCGAACCCGAATGACAAgcg                                                                     <  2:2593407/99‑1 (MQ=255)

                         aaGGTATTGTCGGTCGACCTGGGAGCACTGACCCGTATGCTGGATCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGGTT‑GCCGAACCCGAATGACAAgcg                                                                     <  2:1648056/99‑1 (MQ=255)

                         aaGGTATTGTCGGTCGACCTGGGAGCACTGACCCGTATGCTGGATCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGGTT‑GCCGAACCCGAATGACAAgcg                                                                     <  1:184481/99‑1 (MQ=255)

                          aGGTATTGTCGGTCGACCTGGGAGCACTGACCCGTATGCTGGATCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGGTT‑GCCGAACCCGAATGACAAgcgc                                                                    <  1:396582/99‑1 (MQ=255)

                          aGGTATTGTCGGTCGACCTGGGAGCACTGACCCGTATGCTGGATCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGGTT‑GCCGAACCCGAATGACAAgcgc                                                                    >  1:194328/1‑99 (MQ=255)

                           ggTATTGTCGGTCGACCTGGGAGCACTGACCCGTATGCTGGATCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGGTT‑GCCGAACCCGAATGACAAGcgcg                                                                   >  2:792702/1‑99 (MQ=255)

                           ggTATTGTCGGTCGACCTGGGAGCACTGACCCGTATGCTGGATCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGGTT‑GCCGAACCCGAATGACAAGcgcg                                                                   >  1:1776396/1‑99 (MQ=255)

                            gTATTGTCGGTCGACCTGGGAGCACTGACCCGTATGCTGGATCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGGTT‑GCCGAACCCGAATGACAAGCgcgg                                                                  >  1:901764/1‑99 (MQ=255)

                             tATTGTCGGTCGACCTGGGAGCACTGACCCGTATGCTGGATCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGGTT‑GCCGAACCCGAATGACAAGCgcggc                                                                 >  2:142741/1‑99 (MQ=255)

                             tATTGTCGGTCGACCTGGGAGCACTGACCCGTATGCTGGATCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGGTT‑GCCGAACCCGAATGACAAGCgcggc                                                                 >  2:2549818/1‑99 (MQ=255)

                              aTTGTCGGTCGACCTGGGAGCACTGACCCGTATGCTGGATCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGGTT‑GCCGAACCCGAATGACAAGCgcggcg                                                                >  1:2070042/1‑99 (MQ=255)

                               ttGTCGGTCGACCTGGGAGCACTGACCCGTATGCTGGATCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGGTT‑GCCGAACCCGAATGACAAGCGCGGCGt                                                               >  2:823267/1‑99 (MQ=255)

                               ttGTCGGTCGACCTGGGAGCACTGACCCGTATGCTGGATCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGGTT‑GCCGAACCCGAATGACAAGCGCGGCGt                                                               >  2:2455283/1‑99 (MQ=255)

                                 gtcggtcgACCTGGGAGCACTGACCCGTATGCTGGATCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGGTT‑GCCGAACCCGAATGACAAGCGCGGCGTAc                                                             <  1:1932108/99‑1 (MQ=255)

                                 gtcggtcgACCTGGGAGCACTGACCCGTATGCTGGATCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGGTT‑GCCGAACCCGAATGACAAGCGCGGCGTAc                                                             >  1:2254010/1‑99 (MQ=255)

                                 gtcggtcgACCTGGGAGCACTGACCCGTATGCTGGATCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGGTT‑GCCGAACCCGAATGACAAGCGCGGCGTAc                                                             <  2:1834265/99‑1 (MQ=255)

                                  tcggtcgACCTGGGAGCACTGACCCGTATGCTGGATCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGGTT‑GCCGAACCCGAATGACAAGCGCGGCGTACt                                                            <  1:2570015/99‑1 (MQ=255)

                                  tcggtcgACCTGGGAGCACTGACCCGTATGCTGGATCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGGTT‑GCCGAACCCGAATGACAAGCGCGGCGTACt                                                            <  2:2055777/99‑1 (MQ=255)

                                   cggtcgACCTGGGAGCACTGACCCGTATGCTGGATCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGGTT‑GCCGAACCCGAATGACAAGCGCGGCGTACTg                                                           <  1:1647721/99‑1 (MQ=255)

                                       cgACCTGGGAGCACTGACCCGTATGCTGGATCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGGTT‑GCCGAACCCGAATGACAAGCGCGGCGTACTGGTaa                                                       <  2:393002/99‑1 (MQ=255)

                                        gACCTGGGAGCACTGACCCGTATGCTGGATCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGGTT‑GCCGAACCCGAATGACAAGCGCGGCGTACTGGTaaa                                                      >  1:2228176/1‑99 (MQ=255)

                                         aCCTGGGAGCACTGACCCGTATGCTGGATCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGGTT‑GCCGAACCCGAATGACAAGCGCGGCGTACTGGTaaaa                                                     >  1:1897631/1‑99 (MQ=255)

                                          ccTGGGAGCACTGACCCGTATGCTGGATCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGGTT‑GCCGAACCCGAATGACAAGCGCGGCGTACTGGTAAAAc                                                    <  2:2157439/99‑1 (MQ=255)

                                            tGGGAGCACTGACCCGTATGCTGGATCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGGTT‑GCCGAACCCGAATGACAAGCGCGGCGTACTGGTAAAACtt                                                  <  2:1401027/99‑1 (MQ=255)

                                             gggAGCACTGACCCGTATGCTGGATCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGGTT‑GCCGAACCCGAATGACAAGCGCGGCGTACTGGTAAAACTTa                                                 >  1:1846887/1‑99 (MQ=255)

                                              ggAGCACTGACCCGTATGCTGGATCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGGTT‑GCCGAACCCGAATGACAAGCGCGGCGTACTGGTAAAACTTac                                                >  1:965102/1‑99 (MQ=255)

                                              ggAGCACTGACCCGTATGCTGGATCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGGTT‑GCCGAACCCGAATGACAAGCGCGGCGTACTGGTAAAACTTac                                                >  2:1189617/1‑99 (MQ=255)

                                               gAGCACTGACCCGTATGCTGGATCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGGTT‑GCCGAACCCGAATGACAAGCGCGGCGTACTGGTAAAACTTacc                                               <  1:444713/99‑1 (MQ=255)

                                                aGCACTGACCCGTATGCTGGATCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGGTT‑GCCGAACCCGAATGACAAGCGCGGCGTACTGGTAAAACTTacca                                              <  2:2180198/99‑1 (MQ=255)

                                                  cACTGACCCGTATGCTGGATCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGGTT‑GCCGAACCCGAATGACAAGCGCGGCGTACTGGTAAAACTTaccacc                                            <  1:394362/99‑1 (MQ=255)

                                                  cACTGACCCGTATGCTGGATCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGGTT‑GCCGAACCCGAATGACAAGCGCGGCGTACTGGTAAAACTTaccacc                                            <  2:1681860/99‑1 (MQ=255)

                                                   aCTGACCCGTATGCTGGATCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGGTT‑GCCGAACCCGAATGACAAGCGCGGCGTACTGGTAAAACTTACCACcg                                           >  1:1094843/1‑99 (MQ=255)

                                                       aCCCGTATGCTGGATCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGGTT‑GCCGAACCCGAATGACAAGCGCGGCGTACTGGTAAAACTTACCACcggcgg                                       >  1:1182826/1‑99 (MQ=255)

                                                       aCCCGTATGCTGGATCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGGTT‑GCCGAACCCGAATGACAAGCGCGGCGTACTGGTAAAACTTACCACcggcgg                                       <  2:632223/99‑1 (MQ=255)

                                                       aCCCGTATGCTGGATCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGGTT‑GCCGAACCCGAATGACAAGCGCGGCGTACTGGTAAAACTTACCACcggcgg                                       >  1:270885/1‑99 (MQ=255)

                                                       aCCCGTATGCTGGATCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGGTT‑GCCGAACCCGAATGACAAGCGCGGCGTACTGGTAAAACTTACCACcggcgg                                       <  2:1181162/99‑1 (MQ=255)

                                                          cGTATGCTGGATCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGGTT‑GCCGAACCCGAATGACAAGCGCGGCGTACTGGTAAAACTTACCACCGgcggcgc                                    >  1:2218068/1‑99 (MQ=255)

                                                          cGTATGCTGGATCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGGTT‑GCCGAACCCGAATGACAAGCGCGGCGTACTGGTAAAACTTACCACCGgcggcgc                                    <  1:1872295/99‑1 (MQ=255)

                                                            tATGCTGGATCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGGTT‑GCCGAACCCGAATGACAAGCGCGGCGTACTGGTAAAACTTACCACCGgcggcgcgg                                  >  2:538569/1‑99 (MQ=255)

                                                             aTGCTGGATCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGGTT‑GCCGAACCCGAATGACAAGCGCGGCGTACTGGTAAAACTTACCACCGgcggcgcggc                                 <  1:1617379/99‑1 (MQ=255)

                                                              tGCTGGATCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGGTT‑GCCGAACCCGAATGACAAGCGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCa                                >  1:2620496/1‑99 (MQ=255)

                                                               gCTGGATCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGGTT‑GCCGAACCCGAATGACAAGCGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCaa                               >  2:83069/1‑99 (MQ=255)

                                                               gCTGGATCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGGTT‑GCCGAACCCGAATGACAAGCGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCaa                               <  2:2572457/99‑1 (MQ=255)

                                                               gCTGGATCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGGTT‑GCCGAACCCGAATGACAAGCGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCaa                               <  1:277923/99‑1 (MQ=255)

                                                                 tGGATCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGGTT‑GCCGAACCCGAATGACAAGCGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCAata                             <  1:52973/99‑1 (MQ=255)

                                                                 tGGATCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGGTT‑GCCGAACCCGAATGACAAGCGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCAata                             <  2:800725/99‑1 (MQ=255)

                                                                 tGGATCGCCTGGTCTGTAAAGGCTGGGTGGAAA‑GGGTCGCCGAACCCGAATGACAAGCGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCAata                             >  1:74321/1‑99 (MQ=255)

                                                                  ggATCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGGTT‑GCCGAACCCGAATGACAAGCGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCAatat                            <  2:2510801/99‑1 (MQ=255)

                                                                   gATCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGGTT‑GCCGAACCCGAATGACAAGCGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCAATAtg                           <  1:1343115/99‑1 (MQ=255)

                                                                   gATCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGGTT‑GCCGAACCCGAATGACAAGCGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCAATAtg                           <  1:1124109/99‑1 (MQ=255)

                                                                    aTCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGGTT‑GCCGAACCCGAATGACAAGCGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCAATAtgt                          >  1:1397020/1‑99 (MQ=255)

                                                                     tCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGGTT‑GCCGAACCCGAATGACAAGCGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCAATAtgtg                         >  2:2476174/1‑99 (MQ=255)

                                                                          tGGTCTGTAAAGGCTGGGTGGAAAGGGTT‑GCCGAACCCGAATGACAAGCGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCAATATGTGAACaa                    <  2:514740/99‑1 (MQ=255)

                                                                          tGGTCTGTAAAGGCTGGGTGGAAAGGGTT‑GCCGAACCCGAATGACAAGCGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCAATATGTGAACaa                    <  2:2396002/99‑1 (MQ=255)

                                                                             tCTGTAAAGGCTGGGTGGAAAGGGTT‑GCCGAACCCGAATGACAAGCGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCAATATGTGAACAATGc                 <  2:224068/99‑1 (MQ=255)

                                                                              cTGTAAAGGCTGGGTGGAAAGGGTT‑GCCGAACCCGAATGACAAGCGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCAATATGTGAACAATGcc                <  1:1364850/99‑1 (MQ=255)

                                                                              cTGTAAAGGCTGGGTGGAAAGGGTT‑GCCGAACCCGAATGACAAGCGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCAATATGTGAACAATGcc                >  2:1410732/1‑99 (MQ=255)

                                                                                gTAAAGGCTGGGTGGAAAGGGTT‑GCCGAACCCGAATGACAAGCGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCAATATGTGAACAATGCCAt              <  2:960967/99‑1 (MQ=255)

                                                                                 tAAAGGCTGGGTGGAAAGGGTT‑GCCGAACCCGAATGACAAGCGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCAATATGTGAACAATGCCATc             >  1:2094152/1‑99 (MQ=255)

                                                                                  aaaGGCTGGGTGGAAAGGGTT‑GCCGAACCCGAATGACAAGCGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCAATATGTGAACAATGCCATCa            >  1:2564179/1‑99 (MQ=255)

                                                                                    aGGCTGGGTGGAAAGGGTT‑GCCGAACCCGAATGACAAGCGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCAATATGTGAACAATGCCATCAAt          <  2:1519218/99‑1 (MQ=255)

                                                                                    aGGCTGGGTGGAAAGGGTT‑GCCGAACCCGAATGACAAGCGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCAATATGTGAACAATGCCATCAAt          <  1:838246/99‑1 (MQ=255)

                                                                                      gCTGGGTGGAAAGGGTT‑GCCGAACCCGAATGACAAGCGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCAATATGTGAACAATGCCATCAATTa        >  1:2633962/1‑99 (MQ=255)

                                                                                       cTGGGTGGAAAGGGTT‑GCCGAACCCGAATGACAAGCGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCAATGTGTGAACAATGCCATCAATTAg       >  2:360765/1‑99 (MQ=255)

                                                                                        tGGGTGGAAAGGGTT‑GCCGAACCCGAATGACAAGCGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCAATATGTGAACAATGCCATCAATTAGt      >  2:2350469/1‑99 (MQ=255)

                                                                                        tGGGTGGAAAGGGTT‑GCCGAACCCGAATGACAAGCGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCAATATGTGAACAATGCCATCAATTAGt      >  2:1466083/1‑99 (MQ=255)

                                                                                        tGGGTGGAAAGGGTT‑GCCGAACCCGAATGACAAGCGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCAATATGTGAACAATGCCATCAATTAGt      <  1:887463/99‑1 (MQ=255)

                                                                                        tGGGTGGAAAGGGTT‑GCCGAACCCGAATGACAAGCGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCAATATGTGAACAATGCCATCAATTAGt      <  1:1984230/99‑1 (MQ=255)

                                                                                         gggTGGAAAGGGTT‑GCCGAACCCGAATGACAAGCGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCAATATGTGAACAATGCCATCAATTAGtt     <  2:680176/99‑1 (MQ=255)

                                                                                         gggTGGAAAGGGTT‑GCCGAACCCGAATGACAAGCGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCAATATGTGAACAATGCCATCAATTAGtt     <  1:1051483/99‑1 (MQ=255)

                                                                                            tGGAAAGGGTT‑GCCGAACCCGAATGACAAGCGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCAATATGTGAACAATGCCATCAATTAGTTGGc  >  2:2611918/1‑99 (MQ=255)

GTGTATTACTCCGGTTGAACTGAAAAAGGTATTGTCGGTCGACCTGGGAGCACTGACCCGTATGCTGGATCGCCTGGTCTGTAAAGGCTGGGTGGAAA‑GGTT‑GCCGAACCCGAATGACAAGCGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCAATATGTGAACAATGCCATCAATTAGTTGGC  >  NC_000913/1619278‑1619467

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`